##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043254_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	909118
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19690843212872	33.0	31.0	34.0	30.0	34.0
2	32.53580723294446	34.0	31.0	34.0	31.0	34.0
3	32.602087957778856	34.0	31.0	34.0	31.0	34.0
4	36.13935374725833	37.0	35.0	37.0	35.0	37.0
5	36.02376809171087	37.0	35.0	37.0	35.0	37.0
6	36.28609157447108	37.0	37.0	37.0	35.0	37.0
7	36.20988584540181	37.0	37.0	37.0	35.0	37.0
8	36.1817266845448	37.0	37.0	37.0	35.0	37.0
9	37.74398262931765	39.0	38.0	39.0	35.0	39.0
10	37.61018371652525	39.0	37.0	39.0	35.0	39.0
11	37.30291887301758	39.0	37.0	39.0	35.0	39.0
12	37.29344595531053	39.0	37.0	39.0	34.0	39.0
13	37.30823611456378	39.0	37.0	39.0	34.0	39.0
14	38.71030603288022	40.0	38.0	41.0	35.0	41.0
15	38.82293827643936	40.0	38.0	41.0	35.0	41.0
16	38.83646237342127	41.0	38.0	41.0	35.0	41.0
17	38.80542019847809	41.0	38.0	41.0	35.0	41.0
18	38.54568493858883	40.0	38.0	41.0	35.0	41.0
19	38.275097402097416	40.0	37.0	41.0	35.0	41.0
20	37.97942621309885	40.0	35.0	41.0	34.0	41.0
21	37.910283373555465	40.0	35.0	41.0	34.0	41.0
22	37.87133243429346	40.0	35.0	41.0	34.0	41.0
23	37.87606999311421	40.0	35.0	41.0	34.0	41.0
24	37.78659645942551	40.0	35.0	41.0	34.0	41.0
25	37.698024898858016	40.0	35.0	41.0	34.0	41.0
26	37.65502718019003	40.0	35.0	41.0	34.0	41.0
27	37.68449640200722	40.0	35.0	41.0	34.0	41.0
28	37.690732116182936	40.0	35.0	41.0	34.0	41.0
29	37.61132218259896	40.0	35.0	41.0	34.0	41.0
30	37.56385419714493	40.0	35.0	41.0	34.0	41.0
31	37.45094696178054	40.0	35.0	41.0	33.0	41.0
32	37.24247017438881	40.0	35.0	41.0	33.0	41.0
33	37.008194755796275	40.0	35.0	41.0	33.0	41.0
34	36.88045006258813	40.0	35.0	41.0	32.0	41.0
35	36.69809639672738	40.0	35.0	41.0	31.0	41.0
36	36.626327935427526	40.0	35.0	41.0	31.0	41.0
37	36.61254644611591	40.0	35.0	41.0	31.0	41.0
38	36.58359970872868	40.0	35.0	41.0	31.0	41.0
39	36.5598679159361	40.0	35.0	41.0	31.0	41.0
40	36.53788947089377	39.0	35.0	41.0	31.0	41.0
41	36.5387144463095	39.0	35.0	41.0	31.0	41.0
42	36.466710592024356	39.0	35.0	41.0	31.0	41.0
43	36.33209330361955	39.0	35.0	41.0	31.0	41.0
44	36.15508547845274	39.0	35.0	41.0	31.0	41.0
45	36.094574081692365	39.0	35.0	41.0	30.0	41.0
46	36.095734547110496	39.0	35.0	41.0	30.0	41.0
47	36.01235373185879	39.0	35.0	41.0	30.0	41.0
48	35.9873624765982	39.0	35.0	41.0	30.0	41.0
49	35.924064862867084	39.0	35.0	41.0	30.0	41.0
50	35.78478811331422	38.0	35.0	40.0	29.0	41.0
51	35.713703831625814	38.0	35.0	40.0	29.0	41.0
52	35.34568119870028	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	9.0
16	20.0
17	41.0
18	118.0
19	274.0
20	509.0
21	876.0
22	1352.0
23	2398.0
24	4418.0
25	7940.0
26	11338.0
27	12147.0
28	11480.0
29	11604.0
30	12976.0
31	15828.0
32	19961.0
33	26252.0
34	46910.0
35	87391.0
36	97533.0
37	69303.0
38	111263.0
39	355606.0
40	1566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.384163551926154	20.362703191444893	25.835810092859234	28.41732316376972
2	33.477282376985166	19.805129807131745	26.63185637068015	20.085731445202935
3	20.72965225636276	21.89572750732028	38.36938659227955	19.005233644037407
4	22.773831339826074	24.043633499721707	35.7623542818424	17.420180878609816
5	19.055722139480245	29.299496874993125	32.06932433413484	19.575456651391786
6	71.00365409110807	1.4880356565374353	23.059822817280047	4.448487435074435
7	71.62953543984389	1.4516267415231026	18.542587430894557	8.376250387738446
8	57.90359447288471	3.242483373995455	28.154211004512064	10.699711148607772
9	31.57125917647654	18.12272994264771	30.62385740904921	19.68215347182654
10	25.151520484689556	18.538187562010652	43.89760185146482	12.412690101834965
11	21.779350975340936	15.36918199837645	41.35370765951175	21.497759366770868
12	16.582995826724364	19.967704962392123	44.50808365910696	18.94121555177656
13	11.90285529491221	23.317105150266524	43.987799163584924	20.79224039123634
14	10.580034714965494	22.402812396190594	45.85785343596761	21.159299452876304
15	11.018481649246853	17.98699398757917	49.57508266253666	21.41944170063732
16	11.000992170433321	25.721743492043935	39.923310285353494	23.353954052169247
17	12.848717108230176	22.79110082519541	42.056476716993835	22.303705349580582
18	14.872216807939123	29.92449824995215	39.74093572011554	15.462349221993184
19	14.272514679062564	23.86466883286878	36.72130570508999	25.141510782978667
20	11.71982074934167	30.921948525933928	35.50067207997202	21.857558644752388
21	12.972023433701677	24.766311963903476	36.47117315903986	25.79049144335499
22	11.16565726341355	27.402603402418606	32.30471731942388	29.127022014743957
23	10.731830191460293	24.125471060962383	43.44980519580516	21.69289355177216
24	16.880206969832297	21.975145140674808	38.64778829590878	22.496859593584112
25	12.02715159088259	26.452451716938835	37.79740363737161	23.722993054806967
26	14.379981476551999	24.03307381440033	36.01611671972175	25.570827989325917
27	20.844818824399034	25.84493982079334	36.344896922071726	16.965344432735904
28	16.548786846151987	24.861789118684264	32.22463970573677	26.364784329426982
29	18.984444263560945	28.96763676442442	30.076183729724853	21.971735242289782
30	22.5258987282179	29.169700742917858	27.12266174468001	21.18173878418423
31	25.073312815278104	26.134011206466045	27.30305636892021	21.48961960933564
32	21.816419870687852	30.26339815073511	26.999025429042216	20.921156549534825
33	18.121079991816245	32.67804619422341	29.4476624596587	19.75321135430164
34	28.280927228368597	23.54259843056677	29.791952199824443	18.384522141240193
35	25.479970696873234	25.562138248280203	27.977776262267383	20.98011479257918
36	24.755862275304196	26.899038408655425	31.99914642543652	16.34595289060386
37	23.72277306136277	23.79119102250753	33.20944035867731	19.27659555745239
38	20.606455927613357	21.15665953154596	32.161391590530606	26.075492950310082
39	23.191598890353067	20.04745258591294	36.832182400964456	19.92876612276954
40	15.758460397880143	19.057702080478002	41.81679385954298	23.36704366209887
41	19.743201652590752	23.628725863969255	31.746923941666537	24.881148541773452
42	21.56848725907968	17.906256393559474	37.604359390090174	22.920896957270674
43	25.184739494763058	21.683873820560148	34.15728211299303	18.974104571683764
44	18.236686546740906	25.130181120602607	34.247479425113134	22.385652907543356
45	20.69599325940087	22.403142386356887	33.593218922076126	23.307645432166122
46	25.788291508913037	18.789970058892244	33.14883216480149	22.272906267393232
47	17.436460393480274	17.561636663227436	38.79353395268821	26.20836899060408
48	19.748041508363052	15.200227033234409	33.41634419294305	31.635387265459492
49	21.659784538420755	15.52559733719935	38.82004316271375	23.994574961666142
50	19.234576809611074	15.350152565453548	39.66734791303219	25.747922711903186
51	16.336823162669752	14.589965219036472	38.23508059459828	30.838131023695492
52	14.793349158195085	14.658053190014938	47.751337010157094	22.79726064163288
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310.0
1	1632.5
2	2955.0
3	35614.0
4	68273.0
5	36851.0
6	5429.0
7	5226.5
8	5024.0
9	5407.5
10	5791.0
11	6025.5
12	6260.0
13	6074.0
14	5453.0
15	5018.0
16	4611.0
17	4204.0
18	3677.5
19	3151.0
20	3001.0
21	2851.0
22	2657.5
23	2464.0
24	2706.0
25	2948.0
26	3357.0
27	3766.0
28	4318.0
29	4870.0
30	4716.5
31	4563.0
32	5493.0
33	6423.0
34	7552.5
35	8682.0
36	10618.5
37	12555.0
38	14399.5
39	17820.0
40	19396.0
41	24408.0
42	29420.0
43	42057.5
44	54695.0
45	74732.0
46	94769.0
47	118839.5
48	142910.0
49	146575.5
50	150241.0
51	126618.5
52	102996.0
53	79198.0
54	55400.0
55	42092.5
56	28785.0
57	22830.0
58	16875.0
59	14444.0
60	12013.0
61	10245.0
62	8477.0
63	6820.5
64	4079.0
65	2994.0
66	2549.0
67	2104.0
68	1750.5
69	1397.0
70	1262.0
71	1127.0
72	1152.5
73	1178.0
74	961.0
75	744.0
76	533.5
77	323.0
78	274.5
79	226.0
80	170.0
81	114.0
82	77.5
83	41.0
84	45.0
85	49.0
86	27.5
87	6.0
88	3.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	909118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.81166383373528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94097558981964	21.015508683985747
2	11.795788617570837	6.797125926065039
3	4.833093208895968	4.177483704354587
4	2.402540201597076	2.7688472254179817
5	1.421135514616309	2.047263935465374
6	0.9100965280783289	1.5732837133945459
7	0.66856923850275	1.3483814504522331
8	0.4999436903971366	1.152336763481546
9	0.4075691456751678	1.0568470692776053
>10	3.415573129957064	21.79259669102524
>50	0.4399200529214915	8.6368799762073
>100	0.24448518421956913	13.067880750875618
>500	0.013028991008566377	2.54070692665723
>1k	0.0061312898863841786	3.414695977214271
>5k	7.664112357980223E-4	1.6225547103317681
>10k+	3.8320561789901116E-4	6.987606495793908
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63289	6.961582544840164	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	7695	0.846424776541659	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT	7001	0.7700870514058681	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	4900	0.5389839382786393	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	3232	0.3555094058197066	No Hit
TCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	2792	0.30711084809672673	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	2628	0.28907138567270696	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2340	0.2573923297085747	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1953	0.21482359825677194	No Hit
AGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1643	0.18072461440649068	No Hit
TCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1630	0.17929465701922082	No Hit
ACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	1601	0.17610475207838805	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	1552	0.17071491269560168	No Hit
TGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1301	0.14310573544908362	No Hit
TGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTTG	1145	0.1259462468018453	Illumina Single End Adapter 1 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	1107	0.1217663713621334	No Hit
GGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1049	0.11538656148046787	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT	1029	0.11318662703851425	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCC	1026	0.11285663687222122	No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	993	0.10922674504299773	No Hit
ATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	948	0.10427689254860203	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	922	0.10141697777406233	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT	922	0.10141697777406233	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0999672209768149E-4	0.0	0.0	0.7876865269414971	0.0
2	1.0999672209768149E-4	0.0	0.0	2.6997595471654945	0.0
3	1.0999672209768149E-4	0.0	0.0	4.590823193468835	0.0
4	1.0999672209768149E-4	0.0	0.0	5.754148526373914	0.0
5	1.0999672209768149E-4	0.0	0.0	11.799018389252	0.0
6	1.0999672209768149E-4	0.0	0.0	13.360751849594882	0.0
7	1.0999672209768149E-4	0.0	0.0	18.45151014499768	0.0
8	1.0999672209768149E-4	0.0	0.0	24.79469111820468	0.0
9	1.0999672209768149E-4	0.0	0.0	29.413783469252618	0.0
10	1.0999672209768149E-4	0.0	0.0	32.0951735638278	0.0
11	1.0999672209768149E-4	0.0	0.0	34.527751073018024	0.0
12	1.0999672209768149E-4	0.0	0.0	36.216200757217436	0.0
13	1.0999672209768149E-4	0.0	0.0	36.906760178546676	0.0
14	1.0999672209768149E-4	0.0	0.0	37.35972668014493	0.0
15	1.0999672209768149E-4	0.0	0.0	38.85414214656403	0.0
16	1.0999672209768149E-4	0.0	0.0	40.57625082772533	0.0
17	1.0999672209768149E-4	0.0	0.0	42.56741149113756	0.0
18	1.0999672209768149E-4	0.0	0.0	43.49600381908619	0.0
19	1.0999672209768149E-4	0.0	0.0	44.56066209226965	0.0
20	2.1999344419536298E-4	0.0	0.0	45.6445697918202	0.0
21	3.2999016629304444E-4	0.0	0.0	46.68040892381407	0.0
22	5.499836104884074E-4	0.0	0.0	47.70183848521314	0.0
23	6.599803325860889E-4	0.0	0.0	48.481935238329896	0.0
24	0.0010999672209768148	0.0	0.0	49.08988712136378	0.0
25	0.0010999672209768148	0.0	0.0	49.70080891589431	0.0
26	0.0010999672209768148	0.0	0.0	50.301061028381355	0.0
27	0.0010999672209768148	0.0	0.0	50.89229340965639	0.0
28	0.0010999672209768148	0.0	0.0	51.45525663335233	0.0
29	0.0010999672209768148	0.0	0.0	52.15043591700967	0.0
30	0.0010999672209768148	0.0	0.0	52.75761782298888	0.0
31	0.0010999672209768148	0.0	0.0	53.25843289869962	0.0
32	0.0010999672209768148	0.0	0.0	53.74395843003879	0.0
33	0.0010999672209768148	0.0	0.0	54.24070362703191	0.0
34	0.0010999672209768148	0.0	0.0	54.80883669666644	0.0
35	0.0010999672209768148	0.0	0.0	55.27610277213739	0.0
36	0.0010999672209768148	0.0	0.0	55.69563027021795	0.0
37	0.0010999672209768148	0.0	0.0	56.168176188349584	0.0
38	0.0010999672209768148	0.0	0.0	56.6037632078564	0.0
39	0.0010999672209768148	0.0	0.0	57.05705969962095	0.0
40	0.0010999672209768148	0.0	0.0	57.513436099604235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGG	35	1.0192525E-7	46.000004	1
GTCTAAA	35	1.0192525E-7	46.000004	32
GCCGATA	35	1.0192525E-7	46.000004	8
AATTCGG	35	1.0192525E-7	46.000004	1
ATGACGG	75	0.0	46.000004	1
ATAACGG	35	1.0192525E-7	46.000004	1
CAGCGAT	50	1.6370905E-11	46.0	20
CCTTACC	20	6.310756E-4	46.0	21
GTACCGG	40	5.6097633E-9	46.0	1
AGATCGC	20	6.310756E-4	46.0	21
ACCGGCC	25	3.416588E-5	46.0	20
GCGACAA	30	1.8609717E-6	46.0	8
GGTAACG	20	6.310756E-4	46.0	44
GGCACTA	40	5.6097633E-9	46.0	7
GCAACGC	25	3.416588E-5	46.0	12
GTCCTCG	25	3.416588E-5	46.0	22
CGTGAAT	20	6.310756E-4	46.0	5
GGCCGAT	60	0.0	46.0	7
CCAATCG	20	6.310756E-4	46.0	23
CACGCGG	55	1.8189894E-12	46.0	1
>>END_MODULE