SRR1043253_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043253_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	886760
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57921	6.5317560557535295	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	7249	0.8174703414678155	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	6824	0.7695430556182056	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	4871	0.5493030808787045	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	3195	0.3603004195047138	No Hit
TCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2752	0.31034327213676755	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCC	2381	0.268505570842167	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	2207	0.24888357616491497	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	1977	0.22294645676394967	No Hit
AGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	1610	0.18155983580675716	No Hit
TCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	1517	0.17107221796201902	No Hit
ACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1442	0.1626144616356173	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	1428	0.161035680454689	No Hit
TGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT	1309	0.14761604041679824	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	1287	0.14513509856105372	No Hit
GGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	1083	0.12213000135324101	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTC	1014	0.11434886553295141	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	1010	0.11389778519554333	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	944	0.10645495962830981	No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	928	0.10465063827867743	No Hit
ATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	915	0.10318462718210114	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATAGT	35	1.2115015E-7	45.000004	31
ACGTTGA	25	3.8900816E-5	45.000004	16
GCAACCG	25	3.8900816E-5	45.000004	35
AAGACGG	35	1.2115015E-7	45.000004	1
TAAACGG	50	2.1827873E-11	45.000004	1
TACGGGT	50	2.1827873E-11	45.000004	3
CCGTCAC	25	3.8900816E-5	45.000004	11
ATCGTCA	25	3.8900816E-5	45.000004	10
ATATCGG	25	3.8900816E-5	45.000004	1
CTAACGG	50	2.1827873E-11	45.000004	1
AAGTTAG	25	3.8900816E-5	45.000004	18
GGGCGTT	35	1.2115015E-7	45.000004	6
TCGTCAG	25	3.8900816E-5	45.000004	11
GTCAAAC	25	3.8900816E-5	45.000004	8
AATTCGG	35	1.2115015E-7	45.000004	1
ATGACCG	35	1.2115015E-7	45.000004	17
ATAACGG	35	1.2115015E-7	45.000004	1
CACGTTG	25	3.8900816E-5	45.000004	15
AGAGGCG	35	1.2115015E-7	45.000004	33
AATACGG	35	1.2115015E-7	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content