FastQCFastQC Report
Thu 26 May 2016
SRR1043252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043252_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences346378
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154754.467662495885998No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT27290.7878675897429975TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT25610.7393656641010687TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT20190.5828892135181796TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT10240.2956307848650896TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC8260.2384678010728164TruSeq Adapter, Index 14 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG8010.23125025261419604No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG7630.22027957895709313No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC7530.217392559573645TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC7340.21190722274509352TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC5750.16600361454826806TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC5700.16456010485654401TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT5490.15849736415130292TruSeq Adapter, Index 14 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC4280.12356442961158041TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT4120.11894519859806339No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT4030.11634688115296006No Hit
CGGTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT3930.11345986176951191No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC3930.11345986176951191TruSeq Adapter, Index 15 (95% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT3750.10826322687930527No Hit
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTG3560.10277789005075379TruSeq Adapter, Index 15 (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC3530.10191178423571935TruSeq Adapter, Index 14 (95% over 21bp)
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTAT3510.10133438035902972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC453.092282E-1046.015
CTTGTAG206.305319E-446.035
ATTTAGG453.092282E-1046.01
CTTAAGT206.305319E-446.043
TGTTTGG405.5915734E-946.01
AGACTTG253.4121775E-546.025
ATGGGCG405.5915734E-946.04
CGGGTAG253.4121775E-546.05
CGTGGCG600.046.026
AGCAAGG600.046.01
AGTCCTC301.857612E-646.039
GGGTGCC453.092282E-1046.06
GCAAGTT301.857612E-646.029
CGAACAG206.305319E-446.045
ACTGCGG405.5915734E-946.01
CAAGTTT301.857612E-646.030
GTATTGG405.5915734E-946.01
CATATAC253.4121775E-546.027
ATAGGTG301.857612E-646.023
CTGTAGC253.4121775E-546.01
Produced by FastQC (version 0.11.5)