##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043252_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	346378
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18612036561213	33.0	31.0	34.0	30.0	34.0
2	32.49971995911981	34.0	31.0	34.0	31.0	34.0
3	32.592632903937314	34.0	31.0	34.0	31.0	34.0
4	36.087029199314046	37.0	35.0	37.0	35.0	37.0
5	35.969888387830636	37.0	35.0	37.0	35.0	37.0
6	36.26322399228589	37.0	37.0	37.0	35.0	37.0
7	36.17019556669303	37.0	37.0	37.0	35.0	37.0
8	36.146366108702054	37.0	37.0	37.0	35.0	37.0
9	37.69061834181155	39.0	38.0	39.0	35.0	39.0
10	37.52897412653228	39.0	37.0	39.0	35.0	39.0
11	37.14766238040522	39.0	35.0	39.0	34.0	39.0
12	37.028725842865306	39.0	37.0	39.0	33.0	39.0
13	37.05906552956596	39.0	37.0	39.0	33.0	39.0
14	38.460854904179826	40.0	37.0	41.0	34.0	41.0
15	38.569813902730544	40.0	37.0	41.0	35.0	41.0
16	38.577452956019144	40.0	37.0	41.0	35.0	41.0
17	38.60281253428335	40.0	37.0	41.0	35.0	41.0
18	38.34900888624566	40.0	37.0	41.0	35.0	41.0
19	38.12504835757467	40.0	37.0	41.0	34.0	41.0
20	37.82577126722829	40.0	35.0	41.0	34.0	41.0
21	37.76823008389678	40.0	35.0	41.0	34.0	41.0
22	37.72185589154046	39.0	35.0	41.0	34.0	41.0
23	37.73654793318283	39.0	35.0	41.0	34.0	41.0
24	37.64289302438377	39.0	35.0	41.0	34.0	41.0
25	37.55643834192703	39.0	35.0	41.0	34.0	41.0
26	37.503054466507685	39.0	35.0	41.0	34.0	41.0
27	37.56317953218738	39.0	35.0	41.0	34.0	41.0
28	37.61514010705068	39.0	35.0	41.0	34.0	41.0
29	37.57118523693768	40.0	35.0	41.0	34.0	41.0
30	37.50749181530005	40.0	35.0	41.0	34.0	41.0
31	37.37519992609231	40.0	35.0	41.0	33.0	41.0
32	37.17811466086183	40.0	35.0	41.0	33.0	41.0
33	37.03885639388183	40.0	35.0	41.0	33.0	41.0
34	36.82052266598918	40.0	35.0	41.0	32.0	41.0
35	36.67176610523763	40.0	35.0	41.0	32.0	41.0
36	36.611805599662794	40.0	35.0	41.0	31.0	41.0
37	36.5349358215591	40.0	35.0	41.0	31.0	41.0
38	36.4898492398478	39.0	35.0	41.0	31.0	41.0
39	36.4001437735653	39.0	35.0	41.0	31.0	41.0
40	36.354644348082154	39.0	35.0	41.0	31.0	41.0
41	36.34619115532742	39.0	35.0	41.0	31.0	41.0
42	36.194963305983634	39.0	35.0	41.0	30.0	41.0
43	36.02777601348816	39.0	35.0	41.0	30.0	41.0
44	35.841190837755285	38.0	35.0	41.0	30.0	41.0
45	35.791230967324715	38.0	35.0	41.0	30.0	41.0
46	35.74760521742143	38.0	35.0	41.0	29.0	41.0
47	35.67404685055055	38.0	35.0	41.0	29.0	41.0
48	35.73131088002125	38.0	35.0	41.0	29.0	41.0
49	35.61642194365693	38.0	35.0	41.0	29.0	41.0
50	35.48048952300666	38.0	35.0	40.0	28.0	41.0
51	35.31170859581151	38.0	35.0	40.0	27.0	41.0
52	35.03101524923638	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	3.0
16	7.0
17	24.0
18	62.0
19	128.0
20	219.0
21	367.0
22	612.0
23	1072.0
24	1966.0
25	3420.0
26	4763.0
27	5052.0
28	4605.0
29	4521.0
30	5215.0
31	6299.0
32	7951.0
33	10534.0
34	19690.0
35	40785.0
36	29133.0
37	25936.0
38	41537.0
39	131952.0
40	523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.16627499437031	20.02003591452113	25.134102050361168	27.679587040747393
2	31.05422399805992	20.82782393800992	27.76302190092904	20.354930163001114
3	22.461010803226532	22.973745445726923	36.15731946024285	18.407924290803688
4	23.850533232480124	24.673333756762844	33.39299840059126	18.08313461016577
5	19.422422902147364	31.27479227895536	29.151389522429255	20.15139529646802
6	74.34363614317306	1.8609726945706715	19.709681330800453	4.085709831455809
7	73.49254282893256	1.864725819769154	15.94212103540063	8.700610315897661
8	60.4911397375122	3.619456201028934	25.671953761497555	10.217450299961314
9	33.6721731749707	19.995207547823476	27.912280803053314	18.420338474152516
10	26.96447233946729	19.175871446800894	42.60085802216076	11.258798191571058
11	24.890148912459797	15.557858755463686	38.760544838298046	20.791447493778474
12	16.88848598929493	24.084959206416112	42.06040799357927	16.966146810709688
13	11.214049391127611	29.859575377189085	38.994104706418995	19.932270525264308
14	9.265600009238462	22.434161522960466	50.11663558309131	18.183602884709767
15	9.201219476987568	19.036139708642004	50.22576491578564	21.536875898584785
16	9.095265865615023	24.00816449081639	38.347123662588274	28.549445980980316
17	13.271050701834412	20.986610004099568	43.348307340535484	22.394031953530536
18	15.634076067186713	29.57924579505627	38.52352054691695	16.263157590840066
19	16.359872740185576	21.817782884594287	35.133871088810494	26.688473286409646
20	10.834117640265836	32.35655844193338	35.503698271830196	21.30562564597059
21	13.385087967480613	22.542713451778116	34.12659002592544	29.945608554815838
22	9.68768224309858	28.9622897528134	31.399511516320324	29.950516487767697
23	10.346211364463102	22.64924446702735	43.56945302530762	23.435091143201937
24	15.537649619779547	22.61200191698087	39.234016017183535	22.61633244605604
25	10.255270253884484	26.275629514576558	39.15577779189209	24.31332243964686
26	13.781764430766389	24.32833494044079	34.94506002113298	26.94484060765984
27	18.219401925064524	24.41581162775927	39.91997182269082	17.44481462448539
28	13.485267540086266	25.585054478055763	32.627360860100815	28.302317121757152
29	15.824908048432636	25.643949673478105	30.35527660532713	28.17586567276213
30	20.812522735277643	23.391785852450216	31.73007523572513	24.065616176547007
31	20.741213356506474	25.054420315377996	27.450068999763268	26.754297328352262
32	14.640363995403865	27.6790096368707	30.821818937692345	26.85880743003308
33	12.05648164721778	23.530362782855725	37.99028806679408	26.422867503132412
34	16.83016819774928	27.420332700113747	32.71108442222081	23.038414679916162
35	16.029309020780765	32.1937305487069	33.140384204539544	18.63657622597278
36	18.795650994000773	25.920237428474096	32.2638851197247	23.020226457800437
37	18.80142503276767	28.793399118881684	35.44422567253117	16.96095017581948
38	18.321313709300245	27.30225360733072	27.492508184699954	26.88392449866908
39	21.530524455941197	22.637985091431904	31.23697232503219	24.594518127594707
40	13.269029788265998	17.180074947023193	37.02140436170889	32.52949090300192
41	19.539924591053705	22.383061279873434	29.539693629503027	28.537320499569834
42	18.38453943379776	19.228703901517996	35.883052618815285	26.503704045868965
43	22.109083140384207	24.998412139339106	32.03205746323381	20.860447257042882
44	14.404783213714497	26.720519201565917	35.630438422763575	23.244259161956013
45	20.25099746519698	23.176702908383326	34.140159017027635	22.43214060939205
46	25.1852022934482	19.899069802354653	31.335997089884458	23.57973081431269
47	17.47310741444318	18.728672144304777	36.55428462546698	27.24393581578507
48	19.354866648574678	14.586376732933385	30.593167002523252	35.46558961596868
49	20.487444352701385	16.91908839475948	37.88895368643505	24.704513566104083
50	19.62076113379025	15.79026381583126	36.45439375479967	28.13458129557882
51	16.997037918112582	14.379954847016844	38.259935677208134	30.363071557662437
52	15.15165512821253	14.838413525108407	45.905051706517156	24.104879640161904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	131.0
1	455.0
2	779.0
3	8644.0
4	16509.0
5	9040.0
6	1571.0
7	1644.0
8	1717.0
9	1969.0
10	2221.0
11	2327.0
12	2433.0
13	2406.0
14	2201.5
15	2024.0
16	1908.0
17	1792.0
18	1629.0
19	1466.0
20	1387.0
21	1308.0
22	1235.5
23	1163.0
24	1191.0
25	1219.0
26	1154.0
27	1089.0
28	1291.0
29	1493.0
30	1583.0
31	1673.0
32	2113.0
33	2553.0
34	2733.5
35	2914.0
36	3285.5
37	3657.0
38	4465.5
39	7519.5
40	9765.0
41	15482.5
42	21200.0
43	30815.5
44	40431.0
45	51056.5
46	61682.0
47	60707.0
48	59732.0
49	50381.0
50	41030.0
51	31169.5
52	21309.0
53	16240.0
54	11171.0
55	8872.5
56	6574.0
57	5974.5
58	5375.0
59	4757.0
60	4139.0
61	3429.0
62	2719.0
63	2372.5
64	1653.5
65	1281.0
66	929.0
67	577.0
68	527.0
69	477.0
70	441.5
71	406.0
72	547.5
73	689.0
74	472.0
75	255.0
76	160.5
77	66.0
78	52.0
79	38.0
80	34.5
81	31.0
82	22.5
83	14.0
84	16.0
85	18.0
86	12.5
87	7.0
88	4.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	346378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.01548626744573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.34209799135354	21.11352276282794
2	12.30155471992664	7.384742935283805
3	4.986904075049273	4.4905305234513175
4	2.5773950004784396	3.0944705697057553
5	1.5491209817805718	2.3248809777623403
6	1.0443368898065994	1.880776774474618
7	0.8089319782708542	1.6996340679560573
8	0.6453495935373011	1.5496385490016438
9	0.44286736259030446	1.1963591316116275
>10	4.725913337369941	30.355666320544934
>50	0.40596174076856706	8.264039148384045
>100	0.15659949213922614	7.849983931841817
>500	0.007979591956138912	1.6678970436419056
>1k	0.003989795978069456	2.4948099200624663
>5k	0.0	0.0
>10k+	9.97448994517364E-4	4.6330473434497375
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15475	4.467662495885998	No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	2729	0.7878675897429975	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	2561	0.7393656641010687	TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	2019	0.5828892135181796	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	1024	0.2956307848650896	TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	826	0.2384678010728164	TruSeq Adapter, Index 14 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	801	0.23125025261419604	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	763	0.22027957895709313	No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	753	0.217392559573645	TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	734	0.21190722274509352	TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	575	0.16600361454826806	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	570	0.16456010485654401	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	549	0.15849736415130292	TruSeq Adapter, Index 14 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	428	0.12356442961158041	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	412	0.11894519859806339	No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	403	0.11634688115296006	No Hit
CGGTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	393	0.11345986176951191	No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	393	0.11345986176951191	TruSeq Adapter, Index 15 (95% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	375	0.10826322687930527	No Hit
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTG	356	0.10277789005075379	TruSeq Adapter, Index 15 (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	353	0.10191178423571935	TruSeq Adapter, Index 14 (95% over 21bp)
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTAT	351	0.10133438035902972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7613070114152747	0.0
2	0.0	0.0	0.0	2.6390244184099454	0.0
3	0.0	0.0	0.0	4.336014412000762	0.0
4	0.0	0.0	0.0	5.638060153935873	0.0
5	0.0	0.0	0.0	11.285647471837127	0.0
6	0.0	0.0	0.0	12.545254028835549	0.0
7	0.0	0.0	0.0	18.12730600673253	0.0
8	0.0	0.0	0.0	24.183695269330038	0.0
9	0.0	0.0	0.0	29.83243739498467	0.0
10	0.0	0.0	0.0	32.24223247434883	0.0
11	0.0	0.0	0.0	34.62315735987851	0.0
12	0.0	0.0	0.0	36.46998365947029	0.0
13	0.0	0.0	0.0	37.129956290526536	0.0
14	0.0	0.0	0.0	37.60140655584362	0.0
15	0.0	0.0	0.0	39.39799871816339	0.0
16	0.0	0.0	0.0	41.39350651600275	0.0
17	0.0	0.0	0.0	44.485792977614054	0.0
18	0.0	0.0	0.0	45.41945504622118	0.0
19	0.0	0.0	0.0	46.86700656508208	0.0
20	2.8870193834481405E-4	0.0	0.0	47.9069109470001	0.0
21	2.8870193834481405E-4	0.0	0.0	48.75656075154889	0.0
22	0.0011548077533792562	0.0	0.0	49.786649267563185	0.0
23	0.0011548077533792562	0.0	0.0	50.47751300602232	0.0
24	0.005196634890206653	0.0	0.0	51.02864500632257	0.0
25	0.005196634890206653	0.0	0.0	51.52174791701552	0.0
26	0.005196634890206653	0.0	0.0	52.044298425419626	0.0
27	0.005196634890206653	0.0	0.0	52.58734677144622	0.0
28	0.005196634890206653	0.0	0.0	53.14425281051337	0.0
29	0.005196634890206653	0.0	0.0	53.86283193505361	0.0
30	0.005196634890206653	0.0	0.0	54.47747836178972	0.0
31	0.005196634890206653	0.0	0.0	54.93276131855949	0.0
32	0.005196634890206653	0.0	0.0	55.44693947075161	0.0
33	0.005196634890206653	0.0	0.0	55.888076032542486	0.0
34	0.005196634890206653	0.0	0.0	56.442961158041214	0.0
35	0.005196634890206653	0.0	0.0	56.85667103568933	0.0
36	0.005196634890206653	0.0	0.0	57.28625951994642	0.0
37	0.005196634890206653	0.0	0.0	57.74818262129812	0.0
38	0.005196634890206653	0.0	0.0	58.15034442141245	0.0
39	0.005196634890206653	0.0	0.0	58.570117039765805	0.0
40	0.005196634890206653	0.0	0.0	58.99364278331765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	45	3.092282E-10	46.0	15
CTTGTAG	20	6.305319E-4	46.0	35
ATTTAGG	45	3.092282E-10	46.0	1
CTTAAGT	20	6.305319E-4	46.0	43
TGTTTGG	40	5.5915734E-9	46.0	1
AGACTTG	25	3.4121775E-5	46.0	25
ATGGGCG	40	5.5915734E-9	46.0	4
CGGGTAG	25	3.4121775E-5	46.0	5
CGTGGCG	60	0.0	46.0	26
AGCAAGG	60	0.0	46.0	1
AGTCCTC	30	1.857612E-6	46.0	39
GGGTGCC	45	3.092282E-10	46.0	6
GCAAGTT	30	1.857612E-6	46.0	29
CGAACAG	20	6.305319E-4	46.0	45
ACTGCGG	40	5.5915734E-9	46.0	1
CAAGTTT	30	1.857612E-6	46.0	30
GTATTGG	40	5.5915734E-9	46.0	1
CATATAC	25	3.4121775E-5	46.0	27
ATAGGTG	30	1.857612E-6	46.0	23
CTGTAGC	25	3.4121775E-5	46.0	1
>>END_MODULE