SRR1043251_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043251_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	343510
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14649	4.264504672353061	No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2555	0.7437920293441239	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	2427	0.7065296497918547	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2235	0.6506360804634509	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	1004	0.2922767896131117	TruSeq Adapter, Index 15 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	909	0.2646211172891619	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	863	0.2512299496375652	No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	737	0.21454979476580013	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCC	734	0.2136764577450438	No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	717	0.20872754796075807	No Hit
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	612	0.1781607522342872	No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	537	0.15632732671537947	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	513	0.14934063054932897	No Hit
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	451	0.13129166545369858	TruSeq Adapter, Index 15 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	437	0.12721609269016912	No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	432	0.1257605309889086	No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	422	0.12284940758638759	No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA	418	0.12168495822537917	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	410	0.11935605950336235	No Hit
CGGTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	395	0.1149893743995808	No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	370	0.10771156589327821	No Hit
GTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	361	0.10509155483100928	No Hit
ATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	344	0.10014264504672353	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	20	7.0263725E-4	45.000004	21
TATTTGA	20	7.0263725E-4	45.000004	11
GTTGAGG	95	0.0	45.000004	1
TGTCATG	20	7.0263725E-4	45.000004	22
GTGTTAT	35	1.2086093E-7	45.000004	10
TTGATAT	20	7.0263725E-4	45.000004	23
AGCAAGG	40	6.7902874E-9	45.000004	1
GTCGCTC	20	7.0263725E-4	45.000004	15
ATCTCGG	35	1.2086093E-7	45.000004	1
CTCACGG	20	7.0263725E-4	45.000004	1
CATATTA	20	7.0263725E-4	45.000004	29
CATATGG	20	7.0263725E-4	45.000004	1
CTGTAGG	75	0.0	45.000004	1
AGTCAGG	35	1.2086093E-7	45.000004	1
CTTCCGT	40	6.7902874E-9	45.000004	44
TTTACGG	40	6.7902874E-9	45.000004	1
TCACAGG	35	1.2086093E-7	45.000004	1
CTGAGGT	20	7.0263725E-4	45.000004	2
GGCGTGT	20	7.0263725E-4	45.000004	7
TGGCGTT	20	7.0263725E-4	45.000004	35

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content