##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043251_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	343510
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.116459491717855	33.0	31.0	34.0	30.0	34.0
2	32.39464935518617	34.0	31.0	34.0	30.0	34.0
3	32.47671974615004	34.0	31.0	34.0	31.0	34.0
4	36.0340659660563	37.0	35.0	37.0	35.0	37.0
5	35.977080725451955	37.0	35.0	37.0	35.0	37.0
6	36.26090361270414	37.0	37.0	37.0	35.0	37.0
7	36.17139821257023	37.0	37.0	37.0	35.0	37.0
8	36.07398620127507	37.0	37.0	37.0	35.0	37.0
9	37.6321009577596	39.0	37.0	39.0	35.0	39.0
10	37.46860353410381	39.0	37.0	39.0	35.0	39.0
11	37.145524147768626	39.0	35.0	39.0	34.0	39.0
12	37.030205816424555	39.0	37.0	39.0	33.0	39.0
13	37.06256877529039	39.0	37.0	39.0	33.0	39.0
14	38.47289744112253	40.0	37.0	41.0	34.0	41.0
15	38.59013129166545	40.0	37.0	41.0	35.0	41.0
16	38.62653488981398	40.0	37.0	41.0	35.0	41.0
17	38.63679077756106	40.0	37.0	41.0	35.0	41.0
18	38.410698378504264	40.0	37.0	41.0	35.0	41.0
19	38.20195045267969	40.0	37.0	41.0	35.0	41.0
20	37.94017350295479	40.0	35.0	41.0	34.0	41.0
21	37.886763121888734	40.0	35.0	41.0	34.0	41.0
22	37.85078163663358	40.0	35.0	41.0	34.0	41.0
23	37.83714593461617	40.0	35.0	41.0	34.0	41.0
24	37.743826962824954	40.0	35.0	41.0	34.0	41.0
25	37.7195161712905	39.0	35.0	41.0	34.0	41.0
26	37.7248289715001	40.0	35.0	41.0	34.0	41.0
27	37.7375331140287	40.0	35.0	41.0	34.0	41.0
28	37.70834619079503	40.0	35.0	41.0	34.0	41.0
29	37.705251666618146	40.0	35.0	41.0	34.0	41.0
30	37.590367092661054	40.0	35.0	41.0	34.0	41.0
31	37.4483857820733	40.0	35.0	41.0	33.0	41.0
32	37.257683910220955	40.0	35.0	41.0	33.0	41.0
33	37.09432330936509	40.0	35.0	41.0	33.0	41.0
34	36.83349829699281	40.0	35.0	41.0	32.0	41.0
35	36.68444295653693	40.0	35.0	41.0	32.0	41.0
36	36.620651509417485	40.0	35.0	41.0	31.0	41.0
37	36.506113359145296	39.0	35.0	41.0	31.0	41.0
38	36.449579342668336	39.0	35.0	41.0	31.0	41.0
39	36.327833250851505	39.0	35.0	41.0	30.0	41.0
40	36.28016069401182	39.0	35.0	41.0	31.0	41.0
41	36.22524235102326	39.0	35.0	41.0	30.0	41.0
42	36.08241099240197	39.0	35.0	41.0	30.0	41.0
43	35.91494570754854	39.0	35.0	41.0	30.0	41.0
44	35.79121422957119	38.0	35.0	40.0	30.0	41.0
45	35.78158132223225	38.0	35.0	41.0	29.0	41.0
46	35.686469098425086	38.0	35.0	41.0	29.0	41.0
47	35.5577333993188	38.0	35.0	40.0	29.0	41.0
48	35.63111699804955	38.0	35.0	41.0	29.0	41.0
49	35.52714913685191	38.0	35.0	40.0	28.0	41.0
50	35.4319845128235	38.0	35.0	40.0	27.0	41.0
51	34.93390003202236	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	3.0
17	14.0
18	51.0
19	122.0
20	194.0
21	344.0
22	550.0
23	896.0
24	1613.0
25	3021.0
26	4499.0
27	5259.0
28	4919.0
29	4646.0
30	5105.0
31	6189.0
32	7758.0
33	10409.0
34	19377.0
35	39695.0
36	28416.0
37	25785.0
38	44014.0
39	130542.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.57503420569998	19.982533259584873	25.183546330528948	27.258886204186194
2	31.118744723588833	20.739134231900092	27.7991907076941	20.342930336816977
3	22.445052545777415	23.023492765858343	35.865331431399376	18.666123256964863
4	23.934383278507177	24.816453669471052	33.035719484148935	18.21344356787284
5	19.426508689703358	31.57928444586766	28.84865069430293	20.145556170126053
6	74.67060638700474	1.744927367471107	19.47133999010218	4.113126255421967
7	73.74254024628104	1.8858257401531253	15.67465284853425	8.696981165031586
8	60.53186224564059	3.5905796046694416	25.523565543943405	10.353992605746559
9	33.57340397659457	19.966230968530756	27.638205583534685	18.82215947133999
10	26.889173532066025	19.053593781840412	42.756251637506914	11.30098104858665
11	25.031876801257607	15.335215859800297	38.68096998631772	20.951937352624377
12	16.991936188175018	23.681697767168348	42.192949259119096	17.133416785537538
13	11.259643096270851	29.694040930395037	38.96800675380629	20.078309219527814
14	9.196238828563944	22.366161101569094	50.16476958458269	18.27283048528427
15	9.190125469418648	18.95228668743268	50.14759395650782	21.709993886640856
16	9.101045093301504	23.918663212133563	38.38927542138511	28.59101627317982
17	13.11839538878053	20.990364181537654	43.404558819248344	22.486681610433465
18	15.471747547378534	29.7301388605863	38.58315623999301	16.214957352042152
19	16.277546505196355	21.743471805769847	35.169281825856594	26.8096998631772
20	10.723996390206981	32.51113504701465	35.38732496870543	21.377543594072954
21	13.32741404908154	22.48144158830893	33.90206980873919	30.28907455387034
22	9.593316060667812	29.000029111234028	31.176093854618497	30.230560973479665
23	10.295479025355885	22.589444266542458	43.33236295886583	23.782713749235832
24	15.736659777007947	22.646793397572125	38.86640854705831	22.75013827836162
25	10.246572152193531	25.948298448371226	39.47075776542167	24.334371634013564
26	13.787953771360367	24.083723909056502	34.74454892142878	27.38377339815435
27	18.447789001775785	24.214142237489447	39.75750342057	17.58056534016477
28	13.760006986696165	25.484556490349625	32.20517597740968	28.550260545544525
29	15.827777939506857	25.769555471456435	30.02328898722017	28.37937760181654
30	20.6576227766295	23.285202759744987	31.585106692672703	24.472067770952812
31	20.8861459637274	24.785886873744577	27.188145905504935	27.139821257023083
32	14.683997554656342	27.559314139326368	30.514104392885216	27.24258391313208
33	12.014206282204302	23.41183662775465	37.86498209659108	26.708974993449974
34	16.91217140694594	27.29585747139821	32.59875986143052	23.193211260225322
35	15.82166458036156	32.5545107857122	33.03950394457221	18.584320689354023
36	18.883293062792934	25.97187854793165	32.11988006171582	23.024948327559606
37	18.933073272976042	28.596838519984864	35.64728828855055	16.822799918488546
38	18.562196151494863	27.0795610025909	27.304008616925273	27.054234228988967
39	21.686122674740183	22.643882274169602	31.018019853861606	24.65197519722861
40	13.399027684783556	17.062094262175773	36.89499577887106	32.6438822741696
41	19.64542516957294	22.17548251870397	29.52112020028529	28.6579721114378
42	18.32639515589066	18.98430904486041	35.9028849232919	26.786410875957035
43	22.374021134755903	24.725044394631887	31.84798113592035	21.052953334691857
44	14.414427527582893	26.464440627638208	35.86212919565661	23.259002649122294
45	20.27422782451748	23.03688393350994	34.08110389799424	22.60778434397834
46	25.35530261127769	19.70102762656109	31.326016709848332	23.617653052312885
47	17.63907892055544	18.495822537917384	36.58437891182207	27.280719629705104
48	19.4463043288405	14.254024628103984	30.257634421123115	36.042036621932404
49	20.533608919682106	16.654828098163083	37.87546214084015	24.936100841314662
50	19.86405053710227	15.343367005327355	36.500247445489215	28.29233501208116
51	17.123518965968966	14.310500422112893	37.926406800384264	30.63957381153387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	454.0
2	800.0
3	8248.0
4	15696.0
5	8673.0
6	1650.0
7	1709.5
8	1769.0
9	2033.5
10	2298.0
11	2415.5
12	2533.0
13	2402.0
14	2271.0
15	2066.0
16	1861.0
17	1731.0
18	1601.0
19	1480.0
20	1359.0
21	1272.0
22	1185.0
23	1055.5
24	926.0
25	1018.0
26	1147.0
27	1184.0
28	1249.5
29	1315.0
30	1724.0
31	2133.0
32	2252.0
33	2371.0
34	2756.5
35	3142.0
36	3658.0
37	4174.0
38	5225.0
39	6276.0
40	10057.5
41	13839.0
42	20038.5
43	26238.0
44	37009.0
45	47780.0
46	56688.0
47	65596.0
48	62203.0
49	58810.0
50	44921.0
51	31032.0
52	23307.5
53	15583.0
54	11848.5
55	8114.0
56	7072.5
57	6031.0
58	5422.0
59	4813.0
60	3887.5
61	2962.0
62	2594.5
63	2227.0
64	1876.5
65	1526.0
66	1218.0
67	910.0
68	760.5
69	611.0
70	514.5
71	418.0
72	530.0
73	642.0
74	517.0
75	234.5
76	77.0
77	70.5
78	64.0
79	51.5
80	39.0
81	27.0
82	15.0
83	18.5
84	22.0
85	14.5
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	343510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.148152336531563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.4280922652743	21.2327685438479
2	12.278656477604176	7.403576119495016
3	4.8621088186582915	4.397507920251111
4	2.6139465639259822	3.15222636835175
5	1.6024916130371012	2.4156080633928356
6	1.0576489918915108	1.9131697755674582
7	0.7772688259903447	1.6403253280675771
8	0.6146307088497726	1.4823984192890658
9	0.47693742981740866	1.294090406021616
>10	4.730460940407831	30.470985656545267
>50	0.3961174842999773	8.275320006251409
>100	0.14866877874230885	7.751306649257954
>500	0.007982216308311885	1.6911613622372017
>1k	0.0039911081541559425	2.4729700389455775
>5k	0.0	0.0
>10k+	9.977770385389856E-4	4.406585342478258
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14649	4.264504672353061	No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2555	0.7437920293441239	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	2427	0.7065296497918547	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2235	0.6506360804634509	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	1004	0.2922767896131117	TruSeq Adapter, Index 15 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	909	0.2646211172891619	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	863	0.2512299496375652	No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	737	0.21454979476580013	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCC	734	0.2136764577450438	No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	717	0.20872754796075807	No Hit
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	612	0.1781607522342872	No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	537	0.15632732671537947	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	513	0.14934063054932897	No Hit
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	451	0.13129166545369858	TruSeq Adapter, Index 15 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	437	0.12721609269016912	No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	432	0.1257605309889086	No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	422	0.12284940758638759	No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA	418	0.12168495822537917	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	410	0.11935605950336235	No Hit
CGGTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	395	0.1149893743995808	No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	370	0.10771156589327821	No Hit
GTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	361	0.10509155483100928	No Hit
ATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	344	0.10014264504672353	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7289452999912667	0.0
2	0.0	0.0	0.0	2.621757736310442	0.0
3	0.0	0.0	0.0	4.341940554860121	0.0
4	0.0	0.0	0.0	5.656312771098367	0.0
5	0.0	0.0	0.0	11.417717097027744	0.0
6	0.0	0.0	0.0	12.679689092020611	0.0
7	0.0	0.0	0.0	18.3389129865215	0.0
8	0.0	0.0	0.0	24.514279060289365	0.0
9	0.0	0.0	0.0	30.44831300398824	0.0
10	0.0	0.0	0.0	32.96381473610666	0.0
11	0.0	0.0	0.0	35.39285610317021	0.0
12	0.0	0.0	0.0	37.251899508020145	0.0
13	0.0	0.0	0.0	37.92960903612704	0.0
14	0.0	0.0	0.0	38.41867776775057	0.0
15	0.0	0.0	0.0	40.26578556665017	0.0
16	0.0	0.0	0.0	42.27329626502868	0.0
17	0.0	0.0	0.0	45.41527175336962	0.0
18	0.0	0.0	0.0	46.38554918342989	0.0
19	0.0	0.0	0.0	47.85683095106402	0.0
20	0.0	0.0	0.0	48.936566621059065	0.0
21	0.0	0.0	0.0	49.786614654595205	0.0
22	5.822246805042066E-4	0.0	0.0	50.86780588629152	0.0
23	5.822246805042066E-4	0.0	0.0	51.5437687403569	0.0
24	0.005822246805042066	0.0	0.0	52.09600884981514	0.0
25	0.005822246805042066	0.0	0.0	52.59672207504876	0.0
26	0.005822246805042066	0.0	0.0	53.12945765771011	0.0
27	0.005822246805042066	0.0	0.0	53.66481325143373	0.0
28	0.005822246805042066	0.0	0.0	54.22374894471777	0.0
29	0.005822246805042066	0.0	0.0	54.94221420045996	0.0
30	0.005822246805042066	0.0	0.0	55.570434630723994	0.0
31	0.005822246805042066	0.0	0.0	56.0347588134261	0.0
32	0.005822246805042066	0.0	0.0	56.54100317312451	0.0
33	0.005822246805042066	0.0	0.0	56.97796279584291	0.0
34	0.005822246805042066	0.0	0.0	57.55290966784082	0.0
35	0.005822246805042066	0.0	0.0	57.943582428459145	0.0
36	0.005822246805042066	0.0	0.0	58.381415388198306	0.0
37	0.005822246805042066	0.0	0.0	58.83846176239411	0.0
38	0.005822246805042066	0.0	0.0	59.25941020639865	0.0
39	0.005822246805042066	0.0	0.0	59.68123198742395	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	20	7.0263725E-4	45.000004	21
TATTTGA	20	7.0263725E-4	45.000004	11
GTTGAGG	95	0.0	45.000004	1
TGTCATG	20	7.0263725E-4	45.000004	22
GTGTTAT	35	1.2086093E-7	45.000004	10
TTGATAT	20	7.0263725E-4	45.000004	23
AGCAAGG	40	6.7902874E-9	45.000004	1
GTCGCTC	20	7.0263725E-4	45.000004	15
ATCTCGG	35	1.2086093E-7	45.000004	1
CTCACGG	20	7.0263725E-4	45.000004	1
CATATTA	20	7.0263725E-4	45.000004	29
CATATGG	20	7.0263725E-4	45.000004	1
CTGTAGG	75	0.0	45.000004	1
AGTCAGG	35	1.2086093E-7	45.000004	1
CTTCCGT	40	6.7902874E-9	45.000004	44
TTTACGG	40	6.7902874E-9	45.000004	1
TCACAGG	35	1.2086093E-7	45.000004	1
CTGAGGT	20	7.0263725E-4	45.000004	2
GGCGTGT	20	7.0263725E-4	45.000004	7
TGGCGTT	20	7.0263725E-4	45.000004	35
>>END_MODULE