FastQCFastQC Report
Thu 26 May 2016
SRR1043250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043250_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences827188
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474065.730982557774049No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT58150.7029840858426379TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT53400.6455606222527406TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT43250.5228557474238021TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC23490.28397413888982914TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT21050.2544766123299661TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG18350.22183590671044554No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC16950.20491109638921262TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC14260.17239128227198652TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT11880.1436191047258906TruSeq Adapter, Index 16 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC11700.14144305768458923TruSeq Adapter, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT10670.1289912329482536No Hit
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC10340.12500181337253444TruSeq Adapter, Index 16 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG8840.1068680880283563No Hit
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTTG8540.10324134295952068TruSeq Adapter, Index 13 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGA206.310423E-446.039
CTAGACT253.416319E-546.09
ATCTCGG253.416319E-546.01
ACCGGCC301.8607661E-646.020
ACGCCCG206.310423E-446.020
GATCGTT206.310423E-446.043
GGTCGGA301.8607661E-646.07
CGAGTAG206.310423E-446.017
CAACGCC301.8607661E-646.013
CGCGGAC253.416319E-546.03
ACGTTCC253.416319E-546.046
CTATCGG501.6370905E-1146.01
TAGACGG850.046.01
GCAACGC301.8607661E-646.012
TATGGCA206.310423E-446.011
CGGTTCG206.310423E-446.036
CGTTGCG206.310423E-446.041
ACCGAGG501.6370905E-1146.01
CGACCCC253.416319E-546.019
GCACGAA253.416319E-546.033
Produced by FastQC (version 0.11.5)