##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043250_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	827188
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25521405049396	33.0	31.0	34.0	31.0	34.0
2	32.583043999671176	34.0	31.0	34.0	31.0	34.0
3	32.66835350609535	34.0	31.0	34.0	31.0	34.0
4	36.17259800674091	37.0	35.0	37.0	35.0	37.0
5	36.06587015285522	37.0	35.0	37.0	35.0	37.0
6	36.31726886753676	37.0	37.0	37.0	35.0	37.0
7	36.24530940971097	37.0	37.0	37.0	35.0	37.0
8	36.21981339187706	37.0	37.0	37.0	35.0	37.0
9	37.74058617871633	39.0	38.0	39.0	35.0	39.0
10	37.57282987664231	39.0	37.0	39.0	35.0	39.0
11	37.329371315831466	39.0	37.0	39.0	35.0	39.0
12	37.3254266261116	39.0	37.0	39.0	34.0	39.0
13	37.336771084686916	39.0	37.0	39.0	34.0	39.0
14	38.7442782051964	40.0	38.0	41.0	35.0	41.0
15	38.837572595347126	40.0	38.0	41.0	35.0	41.0
16	38.84446945555303	41.0	38.0	41.0	35.0	41.0
17	38.8431190974724	40.0	38.0	41.0	35.0	41.0
18	38.59142298969521	40.0	38.0	41.0	35.0	41.0
19	38.3290727138208	40.0	37.0	41.0	35.0	41.0
20	38.037009724512444	40.0	35.0	41.0	34.0	41.0
21	37.96345933451646	40.0	35.0	41.0	34.0	41.0
22	37.91150258466032	40.0	35.0	41.0	34.0	41.0
23	37.9101002432337	40.0	35.0	41.0	34.0	41.0
24	37.841225936546465	40.0	35.0	41.0	34.0	41.0
25	37.762483256526934	40.0	35.0	41.0	34.0	41.0
26	37.703742075562026	40.0	35.0	41.0	34.0	41.0
27	37.74151341653892	40.0	35.0	41.0	34.0	41.0
28	37.76642311058671	40.0	35.0	41.0	34.0	41.0
29	37.72941580390431	40.0	35.0	41.0	34.0	41.0
30	37.693061311334304	40.0	35.0	41.0	34.0	41.0
31	37.56998892633839	40.0	35.0	41.0	34.0	41.0
32	37.390928059884814	40.0	35.0	41.0	33.0	41.0
33	37.234957470369494	40.0	35.0	41.0	33.0	41.0
34	37.028929336499075	40.0	35.0	41.0	33.0	41.0
35	36.87132187604269	40.0	35.0	41.0	32.0	41.0
36	36.81381862406128	40.0	35.0	41.0	32.0	41.0
37	36.72895037162048	40.0	35.0	41.0	32.0	41.0
38	36.68946116239597	40.0	35.0	41.0	32.0	41.0
39	36.665802695396934	40.0	35.0	41.0	31.0	41.0
40	36.640005657722305	40.0	35.0	41.0	32.0	41.0
41	36.63658442820737	40.0	35.0	41.0	32.0	41.0
42	36.565795200123794	40.0	35.0	41.0	31.0	41.0
43	36.45406606478817	39.0	35.0	41.0	31.0	41.0
44	36.2853426306958	39.0	35.0	41.0	31.0	41.0
45	36.21141264138261	39.0	35.0	41.0	31.0	41.0
46	36.19400305613718	39.0	35.0	41.0	31.0	41.0
47	36.13384260893533	39.0	35.0	41.0	30.0	41.0
48	36.116977035450226	39.0	35.0	41.0	30.0	41.0
49	36.076263195307476	39.0	35.0	41.0	30.0	41.0
50	35.95263712723105	39.0	35.0	41.0	30.0	41.0
51	35.85512362365024	39.0	35.0	41.0	29.0	41.0
52	35.548773676600725	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	6.0
16	6.0
17	53.0
18	94.0
19	241.0
20	476.0
21	812.0
22	1260.0
23	2134.0
24	4166.0
25	7410.0
26	10496.0
27	11273.0
28	10240.0
29	9714.0
30	11153.0
31	13417.0
32	17159.0
33	23199.0
34	41894.0
35	78437.0
36	77737.0
37	62504.0
38	102871.0
39	339370.0
40	1061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.688989685537024	19.36077409246749	24.921783198015447	28.02845302398004
2	32.203199272716724	20.551918064575407	26.674951764290583	20.569930898417287
3	22.09666968089479	21.65855887657945	37.4282508933882	18.81652054913756
4	22.94037147540825	24.7925501820626	33.71676088144412	18.550317461085026
5	20.0313592556952	30.209819291382374	30.340623896865043	19.41819755605739
6	74.54242566381524	1.453115857580139	20.17546192643027	3.8289965521743548
7	75.14857565631998	1.3371809068796936	16.601667335599647	6.9125761012006945
8	63.396833609771896	3.48203794058908	24.471220568963766	8.649907880675253
9	31.08217237193963	23.890336900438594	27.586352800088974	17.4411379275328
10	23.640333273693525	20.461853895365987	43.653921478551425	12.243891352389058
11	21.73061021194698	17.258349976063485	39.59909960009091	21.411940211898624
12	16.255917639037317	21.659284225593215	43.59577266594776	18.4890254694217
13	12.154915206700291	25.3310009332824	42.10034478256454	20.413739077452767
14	10.969211352195632	23.26073395648873	45.432356368806126	20.337698322509514
15	12.051673863740769	19.996179828527495	47.25225704434784	20.6998892633839
16	12.278345430542997	24.570956058356746	39.04045996798793	24.110238543112327
17	13.723724231976286	22.2774024768251	41.70019390996968	22.298679381228933
18	15.52645831419218	28.103768429909525	39.263021223712144	17.106752032186154
19	15.727621774010261	23.43203721524007	36.47852725136245	24.361813759387225
20	12.786694197691457	30.306774276222576	36.17690295313762	20.72962857294835
21	14.2838145621068	23.48776819779784	36.79417496385344	25.43424227624192
22	12.238209451781215	26.26089836893185	33.804649003612234	27.696243175674695
23	11.102312896221898	23.138875322175853	43.354110552861016	22.40470122874123
24	15.957678302876735	22.246937818246877	39.011808681944125	22.783575196932258
25	12.057113981344024	25.722689400716646	38.783323742607486	23.436872875331847
26	13.85005585187406	22.301338994279416	38.08215302929926	25.76645212454726
27	18.2485722713579	22.851153546714894	39.468415886110535	19.43185829581667
28	13.187449527797792	23.416200428439485	35.506196898407616	27.890153145355107
29	15.619182096452075	23.15314051944661	37.97673563929844	23.250941744802876
30	19.810974047012287	21.75333781437835	31.519436935738916	26.916251202870452
31	17.8404425596116	24.77647161225743	33.578219219814606	23.80486660831637
32	13.926942847333375	22.325638186240614	37.1503213296131	26.59709763681291
33	15.942204190583034	23.38851627441404	33.95830210302858	26.710977431974353
34	12.638481215878372	22.344739043603145	35.75982727022152	29.25695247029696
35	17.84334395566667	26.29619868760185	34.75969187173895	21.10076548499253
36	18.066509668902352	21.656382829538146	32.683380319830555	27.593727181728944
37	16.708535423628	25.39737036804209	34.47136563876652	23.42272856956339
38	15.122438913523892	25.011605584220277	30.63390668143155	29.232048820824286
39	20.17207696436602	21.49148682040842	33.62609225472323	24.71034396050233
40	14.107433860259095	18.853876023346565	37.82247808237064	29.2162120340237
41	16.94391117859543	23.500945371547942	31.210196472869526	28.344946976987096
42	17.345391857715537	18.984559737326943	36.755489683119194	26.914558721838322
43	21.729884862933215	21.76155843653438	34.229703525679774	22.278853174852635
44	15.720730958379473	23.357688941328934	35.774455141999155	25.147124958292434
45	19.092999414885128	22.01567237435746	33.518136143174225	25.373192067583183
46	21.17728980594496	19.971759745064	33.29871806650967	25.55223238248137
47	15.699333162473344	19.41215298094266	37.61115973635981	27.277354120224178
48	17.53132298824451	16.906313921381837	32.53480466351059	33.02755842686306
49	19.266236937673177	16.976310101210366	37.918465935192486	25.838987025923977
50	18.5309748207179	16.482951880346427	37.47962978186337	27.5064435170723
51	15.67092365943413	15.890825302107864	36.88375556705368	31.554495471404326
52	14.689647335309505	16.011233238393206	44.32632001431355	24.972799411983733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	208.0
1	1212.5
2	2217.0
3	27320.5
4	52424.0
5	28748.5
6	5073.0
7	4735.0
8	4397.0
9	4654.5
10	4912.0
11	5166.5
12	5421.0
13	5252.5
14	4957.5
15	4831.0
16	4421.5
17	4012.0
18	3745.5
19	3479.0
20	3297.5
21	3116.0
22	2941.0
23	2766.0
24	2916.5
25	3067.0
26	4141.0
27	5215.0
28	5409.5
29	5604.0
30	6034.5
31	6465.0
32	7107.0
33	7749.0
34	10041.5
35	12334.0
36	14224.0
37	16114.0
38	17707.0
39	21573.0
40	23846.0
41	30373.5
42	36901.0
43	45913.0
44	54925.0
45	69510.5
46	84096.0
47	101318.0
48	118540.0
49	120359.5
50	122179.0
51	104148.0
52	86117.0
53	66656.0
54	47195.0
55	35815.5
56	24436.0
57	20298.0
58	16160.0
59	13615.5
60	11071.0
61	9590.0
62	8109.0
63	6733.0
64	4642.5
65	3928.0
66	3138.0
67	2348.0
68	2181.0
69	2014.0
70	1786.5
71	1559.0
72	1762.0
73	1965.0
74	1613.5
75	1262.0
76	1006.0
77	750.0
78	572.5
79	395.0
80	272.5
81	150.0
82	109.5
83	69.0
84	40.0
85	11.0
86	8.5
87	6.0
88	6.5
89	4.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	827188.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.41689213770169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77903659193385	21.832952137649944
2	12.072150319032819	7.343945882482846
3	4.980261642572765	4.544522435990066
4	2.579110666473715	3.137941238133076
5	1.5093430682897169	2.295476265347802
6	0.97781406366804	1.7845238943191126
7	0.7237603783817421	1.5410178953945726
8	0.4991848781689509	1.2146922076829392
9	0.3901466317426868	1.0680343214044434
>10	3.8518580686839394	25.68267419012132
>50	0.4500470368535325	9.091728007793945
>100	0.17331003821332	9.406442101189095
>500	0.008785301476251244	1.733053328939929
>1k	0.003993318852841474	2.209922362400692
>5k	7.986637705682948E-4	1.3549348110684685
>10k+	3.993318852841474E-4	5.758138920081741
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47406	5.730982557774049	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	5815	0.7029840858426379	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT	5340	0.6455606222527406	TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	4325	0.5228557474238021	TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2349	0.28397413888982914	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	2105	0.2544766123299661	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	1835	0.22183590671044554	No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1695	0.20491109638921262	TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1426	0.17239128227198652	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1188	0.1436191047258906	TruSeq Adapter, Index 16 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1170	0.14144305768458923	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	1067	0.1289912329482536	No Hit
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1034	0.12500181337253444	TruSeq Adapter, Index 16 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	884	0.1068680880283563	No Hit
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTTG	854	0.10324134295952068	TruSeq Adapter, Index 13 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6610347345464392	0.0
2	0.0	0.0	0.0	2.326073395648873	0.0
3	0.0	0.0	0.0	3.8055436007292176	0.0
4	0.0	0.0	0.0	4.765784803454595	0.0
5	0.0	0.0	0.0	9.596609235143644	0.0
6	0.0	0.0	0.0	10.714492956861076	0.0
7	0.0	0.0	0.0	14.820089266285294	0.0
8	0.0	0.0	0.0	19.668926531816226	0.0
9	0.0	0.0	0.0	23.544828986880855	0.0
10	0.0	0.0	0.0	25.76850728008627	0.0
11	0.0	0.0	0.0	27.84046673791206	0.0
12	0.0	0.0	0.0	29.42390363496569	0.0
13	0.0	0.0	0.0	30.041175645681513	0.0
14	0.0	0.0	0.0	30.505157231487885	0.0
15	0.0	0.0	0.0	31.986078134595765	0.0
16	0.0	0.0	0.0	33.763062326822926	0.0
17	0.0	0.0	0.0	35.889422960681244	0.0
18	0.0	0.0	0.0	36.91337398511584	0.0
19	0.0	0.0	0.0	38.08009787376026	0.0
20	0.0	0.0	0.0	39.283089213093035	0.0
21	0.0	0.0	0.0	40.428536197333614	0.0
22	9.671320183561658E-4	0.0	0.0	41.61931749493465	0.0
23	9.671320183561658E-4	0.0	0.0	42.47003099658119	0.0
24	0.0018133725344178107	0.0	0.0	43.219679202309514	0.0
25	0.0018133725344178107	0.0	0.0	43.89751785567489	0.0
26	0.0018133725344178107	0.0	0.0	44.5746311600265	0.0
27	0.0018133725344178107	0.0	0.0	45.23324806452705	0.0
28	0.0019342640367123315	0.0	0.0	45.899602025174445	0.0
29	0.0019342640367123315	0.0	0.0	46.687693728632425	0.0
30	0.0019342640367123315	0.0	0.0	47.38427056485345	0.0
31	0.0026596130504794556	0.0	0.0	47.97204504900942	0.0
32	0.0026596130504794556	0.0	0.0	48.553654066548354	0.0
33	0.0026596130504794556	0.0	0.0	49.13598843310106	0.0
34	0.0026596130504794556	0.0	0.0	49.78964878600753	0.0
35	0.0026596130504794556	0.0	0.0	50.38636924133329	0.0
36	0.0026596130504794556	0.0	0.0	50.93038100165863	0.0
37	0.0026596130504794556	0.0	0.0	51.485393888692776	0.0
38	0.0026596130504794556	0.0	0.0	52.010546574660175	0.0
39	0.0026596130504794556	0.0	0.0	52.56640570221038	0.0
40	0.0026596130504794556	0.0	0.0	53.11513223112521	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGA	20	6.310423E-4	46.0	39
CTAGACT	25	3.416319E-5	46.0	9
ATCTCGG	25	3.416319E-5	46.0	1
ACCGGCC	30	1.8607661E-6	46.0	20
ACGCCCG	20	6.310423E-4	46.0	20
GATCGTT	20	6.310423E-4	46.0	43
GGTCGGA	30	1.8607661E-6	46.0	7
CGAGTAG	20	6.310423E-4	46.0	17
CAACGCC	30	1.8607661E-6	46.0	13
CGCGGAC	25	3.416319E-5	46.0	3
ACGTTCC	25	3.416319E-5	46.0	46
CTATCGG	50	1.6370905E-11	46.0	1
TAGACGG	85	0.0	46.0	1
GCAACGC	30	1.8607661E-6	46.0	12
TATGGCA	20	6.310423E-4	46.0	11
CGGTTCG	20	6.310423E-4	46.0	36
CGTTGCG	20	6.310423E-4	46.0	41
ACCGAGG	50	1.6370905E-11	46.0	1
CGACCCC	25	3.416319E-5	46.0	19
GCACGAA	25	3.416319E-5	46.0	33
>>END_MODULE