##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043249_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	808412
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20317362928804	33.0	31.0	34.0	30.0	34.0
2	32.48980346654923	34.0	31.0	34.0	31.0	34.0
3	32.5683822110508	34.0	31.0	34.0	31.0	34.0
4	36.1251614275889	37.0	35.0	37.0	35.0	37.0
5	36.08056040731706	37.0	35.0	37.0	35.0	37.0
6	36.322521189690406	37.0	37.0	37.0	35.0	37.0
7	36.25834599189522	37.0	37.0	37.0	35.0	37.0
8	36.15279214064116	37.0	37.0	37.0	35.0	37.0
9	37.677214093803656	39.0	38.0	39.0	35.0	39.0
10	37.51706431868899	39.0	37.0	39.0	35.0	39.0
11	37.31841437286928	39.0	37.0	39.0	35.0	39.0
12	37.32060508750489	39.0	37.0	39.0	34.0	39.0
13	37.33960035229561	39.0	37.0	39.0	34.0	39.0
14	38.74781423333647	40.0	38.0	41.0	35.0	41.0
15	38.84999604162234	40.0	38.0	41.0	35.0	41.0
16	38.88490274760889	40.0	38.0	41.0	35.0	41.0
17	38.85948996303865	40.0	38.0	41.0	35.0	41.0
18	38.64110503060321	40.0	38.0	41.0	35.0	41.0
19	38.40719212480765	40.0	37.0	41.0	35.0	41.0
20	38.13739281455496	40.0	35.0	41.0	34.0	41.0
21	38.07293929333063	40.0	35.0	41.0	34.0	41.0
22	38.02685264444367	40.0	35.0	41.0	34.0	41.0
23	37.99071760438984	40.0	35.0	41.0	34.0	41.0
24	37.92273370509097	40.0	35.0	41.0	34.0	41.0
25	37.91319648891902	40.0	35.0	41.0	34.0	41.0
26	37.892584474253226	40.0	35.0	41.0	34.0	41.0
27	37.8946787034334	40.0	35.0	41.0	34.0	41.0
28	37.83754570689203	40.0	35.0	41.0	34.0	41.0
29	37.85784476232416	40.0	35.0	41.0	34.0	41.0
30	37.782273147850354	40.0	35.0	41.0	34.0	41.0
31	37.65369390855158	40.0	35.0	41.0	34.0	41.0
32	37.487677075550586	40.0	35.0	41.0	33.0	41.0
33	37.30276270020732	40.0	35.0	41.0	33.0	41.0
34	37.04485089286156	40.0	35.0	41.0	33.0	41.0
35	36.90584998738267	40.0	35.0	41.0	32.0	41.0
36	36.83316675160685	40.0	35.0	41.0	32.0	41.0
37	36.697515623221825	40.0	35.0	41.0	32.0	41.0
38	36.649086109558	40.0	35.0	41.0	31.0	41.0
39	36.57926403863377	40.0	35.0	41.0	31.0	41.0
40	36.53058588937324	39.0	35.0	41.0	31.0	41.0
41	36.49356763630426	39.0	35.0	41.0	31.0	41.0
42	36.410894444911754	39.0	35.0	41.0	31.0	41.0
43	36.292311346194765	39.0	35.0	41.0	31.0	41.0
44	36.176330880788505	39.0	35.0	41.0	30.0	41.0
45	36.13436836662494	39.0	35.0	41.0	30.0	41.0
46	36.05026273731711	39.0	35.0	41.0	30.0	41.0
47	35.92457558769539	39.0	35.0	40.0	30.0	41.0
48	35.94103996477044	39.0	35.0	40.0	30.0	41.0
49	35.88488171872758	39.0	35.0	40.0	30.0	41.0
50	35.78961470141463	38.0	35.0	40.0	29.0	41.0
51	35.350427752185766	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	7.0
16	18.0
17	47.0
18	98.0
19	209.0
20	368.0
21	658.0
22	1099.0
23	1769.0
24	3349.0
25	6152.0
26	9549.0
27	11246.0
28	10913.0
29	10368.0
30	11188.0
31	13403.0
32	16864.0
33	22343.0
34	41656.0
35	76332.0
36	73514.0
37	61979.0
38	109276.0
39	325902.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.032859482541078	19.522471215172462	24.8959688871516	27.548700415134856
2	32.00397816954721	20.45838013290253	26.803041023636464	20.734600673913796
3	22.194870932148458	21.923350964607156	36.89418266923302	18.98759543401137
4	23.111977555998674	25.002597685338664	33.15809265572505	18.72733210293761
5	20.25761616601436	30.448088350989348	29.826251960633932	19.46804352236236
6	75.25395466667986	1.380484208547127	19.552653844821698	3.8129072799513124
7	75.74392760127262	1.2601247878556974	16.031429518611798	6.964518092259888
8	63.76785599422077	3.4003701083111086	23.974161689831423	8.8576122076367
9	31.262153456405894	23.98331543816767	27.1537285443561	17.600802561070346
10	23.90785886404457	20.539279476306636	43.255295567111816	12.297566092536973
11	22.05719360919927	17.090171843070117	39.36915830047055	21.48347624726006
12	16.457697312756366	21.544954800275107	43.301435406698566	18.69591248026996
13	12.23546904301272	25.302197394397908	41.79180912702929	20.670524435560086
14	11.098425060488958	23.105668891604775	45.20999193480552	20.585914113100745
15	12.158775475871215	19.8954246102235	47.0894791269798	20.85632078692548
16	12.243509497632395	24.531921841833125	38.932499764971325	24.292068895563155
17	13.855187701320615	22.298654646393175	41.433452249595504	22.412705402690708
18	15.503975695561174	28.237458127786326	39.0941747524777	17.164391424174802
19	15.813223950164026	23.512268496756604	36.1704180541605	24.50408949891887
20	12.750305537275548	30.50449523262891	35.7921455891303	20.95305364096525
21	14.266611579244245	23.59094125272757	36.39567448281322	25.746772685214964
22	12.35471516998758	26.362053012572794	33.31803585300565	27.965195964433974
23	11.200105886602376	23.146737059816033	42.97326115891401	22.67989589466757
24	15.94409781151195	22.37052394076288	38.78220511323434	22.903173134490828
25	12.03903455168899	25.682696446861254	38.58193594355354	23.696333057896222
26	13.915924058524615	22.25016452007145	37.82279332815446	26.01111809324948
27	18.450987862624505	22.778360538933118	39.18274345259595	19.587908145846423
28	13.367070256255472	23.494826895197004	35.06640178522832	28.0717010633192
29	15.74296274671826	23.060023849225395	37.48484683552446	23.712166568531888
30	19.983498513134393	21.666180116079424	31.11569348302598	27.234627887760197
31	17.993671543717806	24.646096297432496	33.11986957145614	24.24036258739356
32	14.00560605235944	22.192149547507952	36.85051681568309	26.951727584449515
33	16.01299832263747	23.207473417020033	33.66587828978293	27.113649970559567
34	12.754882411443669	22.38153317862674	35.36983617264464	29.49374823728495
35	17.886053151116016	26.279792976848437	34.601045011701956	21.23310886033359
36	18.259130245468892	21.513782576211142	32.34105876706432	27.886028411255648
37	16.86268882698426	25.328050548482707	34.170447741992945	23.63881288254009
38	15.211921643914241	24.926893712611886	30.190793803159778	29.6703908403141
39	20.264543326917465	21.376476351167472	33.345868195919905	25.013112125995164
40	14.083041815311995	18.684779543104256	37.528388989772544	29.703789651811206
41	17.10860303904445	23.32770913840962	31.036649629149494	28.527038193396436
42	17.556394511709374	18.797840704987063	36.396664077227946	27.249100706075613
43	21.790992711637138	21.652078395669534	34.15275874182966	22.40417015086367
44	15.876929090612213	23.200175158211405	35.645933014354064	25.276962736822313
45	19.265547765248414	21.87622153060568	33.43047851837924	25.42775218576666
46	21.424224281678153	19.621925453852736	33.04750547987907	25.906344784590036
47	15.981578699969818	19.181061142090915	37.330470106826716	27.506890051112553
48	17.69803021231748	16.597724922440538	32.2219363393913	33.48230852585068
49	19.520615725644845	16.820878462961954	37.68796108914761	25.970544722245588
50	18.671296319203577	16.184692953592968	37.346674715367904	27.79733601183555
51	15.885959139646616	15.726139641667864	36.53434140017714	31.853559818508387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	1148.0
2	2136.0
3	25220.0
4	48304.0
5	26691.5
6	5079.0
7	4792.0
8	4505.0
9	4654.5
10	4804.0
11	4945.5
12	5087.0
13	4922.5
14	4758.0
15	4467.0
16	4176.0
17	3884.5
18	3593.0
19	3371.0
20	3149.0
21	3021.0
22	2893.0
23	2682.0
24	2471.0
25	2863.5
26	4147.5
27	5039.0
28	5254.0
29	5469.0
30	6269.5
31	7070.0
32	7640.0
33	8210.0
34	11047.0
35	13884.0
36	15297.5
37	16711.0
38	18697.5
39	20684.0
40	24983.0
41	29282.0
42	36203.5
43	43125.0
44	51259.0
45	59393.0
46	75179.0
47	90965.0
48	109477.5
49	127990.0
50	124256.0
51	120522.0
52	94122.0
53	67722.0
54	51002.5
55	34283.0
56	26765.0
57	19247.0
58	16251.0
59	13255.0
60	11076.0
61	8897.0
62	7511.0
63	6125.0
64	5357.0
65	4589.0
66	3694.0
67	2799.0
68	2395.0
69	1991.0
70	1949.5
71	1908.0
72	1919.5
73	1931.0
74	1586.0
75	1057.5
76	874.0
77	702.5
78	531.0
79	361.5
80	192.0
81	133.0
82	74.0
83	47.0
84	20.0
85	15.0
86	10.0
87	7.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	808412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.031846169823485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96777928684746	21.312934305565886
2	12.43938779358241	7.47155561327294
3	5.073093609154303	4.570631006257099
4	2.6353739254761153	3.165805773194502
5	1.5254736190734317	2.2906394532068606
6	1.0468747941403225	1.886374966601268
7	0.7160819920581593	1.505368496132998
8	0.5360310681643023	1.2878402065085195
9	0.41628282344354717	1.1251567545116758
>10	4.008757517382051	26.36290627177786
>50	0.4409582160964495	8.81630592929841
>100	0.17937283336014884	9.477073718163398
>500	0.009134727624822394	1.7786736952919397
>1k	0.004152148920373815	2.2056351880485017
>5k	8.304297840747631E-4	1.3138044064495145
>10k+	4.1521489203738153E-4	5.429294215718666
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43540	5.385867602163254	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	5453	0.6745322929397386	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	5083	0.6287635512585167	TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	4238	0.524237641202753	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	2246	0.27782863193520135	No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2033	0.2514806806430384	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC	1730	0.2139997921851729	No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1632	0.20187726060474112	No Hit
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1426	0.17639520442546622	No Hit
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1170	0.1447281831541343	No Hit
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1123	0.13891431596760068	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	1059	0.1309975606497677	No Hit
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1031	0.12753398019821577	No Hit
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT	965	0.1193698262767005	TruSeq Adapter, Index 13 (95% over 23bp)
CGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	917	0.11343225978832576	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6444733625923416	0.0
2	0.0	0.0	0.0	2.271242881105179	0.0
3	0.0	0.0	0.0	3.7430171744110674	0.0
4	0.0	0.0	0.0	4.704284449018569	0.0
5	1.2369930184114042E-4	0.0	0.0	9.527072829200952	0.0
6	1.2369930184114042E-4	0.0	0.0	10.67104397262782	0.0
7	1.2369930184114042E-4	0.0	0.0	14.870016773625329	0.0
8	1.2369930184114042E-4	0.0	0.0	19.905939050879997	0.0
9	1.2369930184114042E-4	0.0	0.0	23.942123570654566	0.0
10	1.2369930184114042E-4	0.0	0.0	26.24837335418079	0.0
11	1.2369930184114042E-4	0.0	0.0	28.40494698247923	0.0
12	1.2369930184114042E-4	0.0	0.0	30.030355808671814	0.0
13	2.4739860368228083E-4	0.0	0.0	30.682498527978307	0.0
14	2.4739860368228083E-4	0.0	0.0	31.170368574439767	0.0
15	2.4739860368228083E-4	0.0	0.0	32.70312662355334	0.0
16	2.4739860368228083E-4	0.0	0.0	34.512228912980014	0.0
17	2.4739860368228083E-4	0.0	0.0	36.7170452689965	0.0
18	2.4739860368228083E-4	0.0	0.0	37.756490502367605	0.0
19	2.4739860368228083E-4	0.0	0.0	38.94709628258858	0.0
20	2.4739860368228083E-4	0.0	0.0	40.164544811309085	0.0
21	2.4739860368228083E-4	0.0	0.0	41.31185583588566	0.0
22	6.184965092057021E-4	0.0	0.0	42.54266388920501	0.0
23	6.184965092057021E-4	0.0	0.0	43.4203104357679	0.0
24	0.0013606923202525445	0.0	0.0	44.16003226077792	0.0
25	0.0013606923202525445	0.0	0.0	44.8632627917448	0.0
26	0.0013606923202525445	0.0	0.0	45.54286675606003	0.0
27	0.0013606923202525445	0.0	0.0	46.19216439142417	0.0
28	0.001484391622093685	0.0	0.0	46.863233103912364	0.0
29	0.001484391622093685	0.0	0.0	47.67148434214237	0.0
30	0.001484391622093685	0.0	0.0	48.37867325076817	0.0
31	0.0028450839423462292	0.0	0.0	48.967234528928316	0.0
32	0.0028450839423462292	0.0	0.0	49.55963048544554	0.0
33	0.0028450839423462292	0.0	0.0	50.13248195227186	0.0
34	0.0028450839423462292	0.0	0.0	50.79402581851828	0.0
35	0.0028450839423462292	0.0	0.0	51.39075125059994	0.0
36	0.0028450839423462292	0.0	0.0	51.9525934795624	0.0
37	0.0028450839423462292	0.0	0.0	52.49278833070266	0.0
38	0.0028450839423462292	0.0	0.0	53.03397277625765	0.0
39	0.0028450839423462292	0.0	0.0	53.589382641524374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	30	2.1646993E-6	45.000004	1
CAGCGAT	20	7.0320425E-4	45.000004	20
TTTACGG	40	6.8102963E-9	45.000004	1
TGCCAAC	20	7.0320425E-4	45.000004	29
TAGACGG	115	0.0	45.000004	1
GACGTTT	60	0.0	45.000004	27
CGTCTTT	20	7.0320425E-4	45.000004	1
ATACTAC	20	7.0320425E-4	45.000004	9
TGGCACG	20	7.0320425E-4	45.000004	31
TCCAGCG	20	7.0320425E-4	45.000004	17
CGTAGTA	20	7.0320425E-4	45.000004	28
CTTGCGT	20	7.0320425E-4	45.000004	12
AACGCCA	40	6.8102963E-9	45.000004	14
ACAGCCC	20	7.0320425E-4	45.000004	38
CTCGAGC	20	7.0320425E-4	45.000004	1
TAAGGTA	20	7.0320425E-4	45.000004	21
CCGGTTA	20	7.0320425E-4	45.000004	29
TGCTACG	20	7.0320425E-4	45.000004	10
TTACCGG	30	2.1646993E-6	45.000004	1
TCGACGT	60	0.0	45.000004	25
>>END_MODULE