FastQCFastQC Report
Thu 26 May 2016
SRR1043248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043248_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1397265
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT564564.040464765094668No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT85420.6113371479282741No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT75120.5376217109853894Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT52560.3761634335648571No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC40810.2920705807416632No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG29230.2091943904699538No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT28980.20740518083541779No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC21260.15215438732094486No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT19970.14292206560673887No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC19280.1379838470154194No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC16920.1210937080653992No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT14180.10148397047088419No Hit
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC14000.10019573953401824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGACG700.046.00000436
CCGTAGG351.01978E-746.0000041
AGTACGG551.8189894E-1246.0000041
GCGGTTT206.311925E-446.018
TCTTCGA206.311925E-446.031
GGCAACG206.311925E-446.08
TCGGGTA301.8616938E-646.04
ACCGAAC253.4175362E-546.010
TCGTCCA206.311925E-446.020
CGTACAA206.311925E-446.016
CCAACGT206.311925E-446.046
CATCCGG453.110472E-1046.01
CGTTTTT161900.045.4885751
TCTACGG1500.044.466671
TTACCGG1250.044.161
CGAGGGT1200.044.0833323
CCCGCAC1100.043.90909232
TACGGGA6300.043.0793653
ATTACGG1550.043.032261
ACGGGAC3100.043.032264
Produced by FastQC (version 0.11.5)