##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043248_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1397265
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24440317334221	33.0	31.0	34.0	31.0	34.0
2	32.5846235324008	34.0	31.0	34.0	31.0	34.0
3	32.689152021985805	34.0	31.0	34.0	31.0	34.0
4	36.19382722676085	37.0	35.0	37.0	35.0	37.0
5	36.0887848761688	37.0	35.0	37.0	35.0	37.0
6	36.32811098825205	37.0	37.0	37.0	35.0	37.0
7	36.257587501297174	37.0	37.0	37.0	35.0	37.0
8	36.229426057333434	37.0	37.0	37.0	35.0	37.0
9	37.78029722350449	39.0	38.0	39.0	35.0	39.0
10	37.516333694753676	39.0	37.0	39.0	35.0	39.0
11	37.321403599174104	39.0	37.0	39.0	34.0	39.0
12	37.370704197127964	39.0	37.0	39.0	34.0	39.0
13	37.38007249877439	39.0	37.0	39.0	34.0	39.0
14	38.79379931509055	40.0	38.0	41.0	35.0	41.0
15	38.860046591018886	40.0	38.0	41.0	35.0	41.0
16	38.86905633505455	41.0	38.0	41.0	35.0	41.0
17	38.842893796094515	41.0	38.0	41.0	35.0	41.0
18	38.60372012467213	40.0	38.0	41.0	35.0	41.0
19	38.352914443573695	40.0	37.0	41.0	35.0	41.0
20	38.07216455003167	40.0	35.0	41.0	34.0	41.0
21	37.99692112806089	40.0	35.0	41.0	34.0	41.0
22	37.94878494773719	40.0	35.0	41.0	34.0	41.0
23	37.93419931079645	40.0	35.0	41.0	34.0	41.0
24	37.85825094022966	40.0	35.0	41.0	34.0	41.0
25	37.76810626473861	40.0	35.0	41.0	34.0	41.0
26	37.6899843623078	40.0	35.0	41.0	34.0	41.0
27	37.72213144965343	40.0	35.0	41.0	34.0	41.0
28	37.74735286434571	40.0	35.0	41.0	34.0	41.0
29	37.67763953151335	40.0	35.0	41.0	34.0	41.0
30	37.668823737802064	40.0	35.0	41.0	34.0	41.0
31	37.48389174566027	40.0	35.0	41.0	33.0	41.0
32	37.241130351078716	40.0	35.0	41.0	33.0	41.0
33	36.97999162649891	40.0	35.0	41.0	32.0	41.0
34	36.70860216208092	40.0	35.0	41.0	31.0	41.0
35	36.488281034735714	40.0	35.0	41.0	30.0	41.0
36	36.45568091951061	40.0	35.0	41.0	30.0	41.0
37	36.34161093278655	40.0	35.0	41.0	30.0	41.0
38	36.27907662469181	40.0	35.0	41.0	30.0	41.0
39	36.23083738589315	40.0	35.0	41.0	30.0	41.0
40	36.19760603750899	40.0	35.0	41.0	30.0	41.0
41	36.158669973126074	40.0	35.0	41.0	29.0	41.0
42	36.10210446837214	40.0	35.0	41.0	29.0	41.0
43	36.00504557116939	39.0	35.0	41.0	28.0	41.0
44	35.83211917567534	39.0	35.0	41.0	27.0	41.0
45	35.76678940644759	39.0	35.0	41.0	26.0	41.0
46	35.74817661646144	39.0	35.0	41.0	26.0	41.0
47	35.65655620086383	39.0	35.0	41.0	26.0	41.0
48	35.59193996843834	39.0	35.0	41.0	26.0	41.0
49	35.534781877453455	39.0	35.0	41.0	26.0	41.0
50	35.38880026337166	39.0	35.0	41.0	26.0	41.0
51	35.27751070841966	38.0	35.0	40.0	25.0	41.0
52	34.91630578308338	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	5.0
15	14.0
16	37.0
17	108.0
18	245.0
19	500.0
20	1009.0
21	1687.0
22	2645.0
23	4560.0
24	8627.0
25	15960.0
26	22877.0
27	24210.0
28	22237.0
29	20850.0
30	21298.0
31	24822.0
32	30355.0
33	38769.0
34	68573.0
35	109479.0
36	111920.0
37	108424.0
38	183099.0
39	574002.0
40	950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.23134122732624	18.850683299159428	26.14765273588045	27.77032273763388
2	32.63826117450877	20.236175671758758	26.113156774126594	21.01240637960587
3	21.423280480080727	22.524646362715732	36.19878834723549	19.853284809968045
4	23.05468182485069	24.36746071790247	33.315942215685645	19.26191524156119
5	21.18910872311265	30.169438152390565	29.910575302465887	18.730877822030894
6	76.33884767742697	1.611576902019302	18.760507133578812	3.2890682869749117
7	76.92341824922259	1.2752770591119078	16.017076216752013	5.784228474913491
8	66.35920888306799	3.567540874494101	22.551412938848394	7.521837303589512
9	34.08580333723381	25.448214905547623	25.321682000193235	15.144299757025331
10	22.762969086035934	20.06856251319542	44.81118470726741	12.357283693501232
11	21.718070659466886	17.294643464196128	41.24643499980319	19.7408508765338
12	18.85225780363782	19.714585279098813	43.30202216472895	18.131134752534415
13	14.794545057666225	23.075365088225926	42.2209101351569	19.909179718950952
14	14.199310796448778	23.173127502656975	43.03285346730935	19.594708233584896
15	15.283500266592235	20.909276336271216	44.535145444851196	19.272077952285358
16	15.65601371250264	24.379913616958845	38.858412684780625	21.105659985757892
17	16.293544889480522	22.280097189867348	40.74903472140217	20.677323199249965
18	17.026906134484154	27.418206281557183	39.56608087943232	15.988806704526343
19	17.146783179998067	23.529466493471173	37.28748662565798	22.036263700872777
20	15.799222051650904	28.261353429735948	36.389732799433176	19.54969171917997
21	15.64513531792466	23.295437873273862	37.707879321388575	23.351547487412912
22	13.956908675161833	25.427817915713913	35.144657598952236	25.470615810172013
23	13.727746705170457	23.04630832376106	41.940362064461645	21.285582906606834
24	17.47993401394868	22.64445183984427	38.47401888689691	21.401595259310152
25	14.76992553309501	25.452079598358225	38.19819432963683	21.57980053890994
26	15.845383660221934	22.92657441501791	37.40607544023503	23.821966484525127
27	18.758717923944275	23.558165416009132	38.28536462303142	19.39775203701517
28	15.211001492200834	22.89987940727063	36.757952142220695	25.131166958307837
29	16.72603264234057	25.427245368630864	36.19771482145477	21.649007167573796
30	18.95510157343095	21.73002257982559	34.536970438678416	24.777905408065042
31	18.485326691787172	24.102371418449614	35.8944795725936	21.517822317169614
32	16.526070573584825	24.037816734835555	35.18344766382898	24.25266502775064
33	15.35914804994042	21.976718804235418	38.54852157607898	24.11561156974518
34	18.855907791292275	20.481941507158627	38.19597570969	22.4661749918591
35	16.670137733357667	20.71031622491081	39.579893577810935	23.039652463920586
36	21.443033354446005	21.1935459630063	37.421963621789715	19.94145706075798
37	19.61317287701331	20.7169005163659	38.381266259442555	21.288660347178237
38	20.810798237986354	20.881293097587072	33.85005707578734	24.457851588639233
39	24.24114251770423	20.38274772501995	34.54570178169495	20.83040797558087
40	19.73576952117172	20.327425363120096	36.55920673601643	23.377598379691754
41	20.556086354413804	22.84462861375616	31.742439694689267	24.856845337140772
42	20.015315634471627	19.091153074041074	36.413636640150585	24.479894651336718
43	21.8946298662029	21.69889033218466	35.57571398410466	20.830765817507775
44	17.275534705299282	23.362640587147034	36.61460066630167	22.747224041252018
45	19.7231019169592	21.172147015777252	35.28307085627995	23.8216802109836
46	22.772988659989334	19.364759011354323	34.383241546879084	23.47901078177726
47	18.406172057555295	18.528768701713705	37.514286838931774	25.550772401799225
48	19.460803784536218	17.26666022551198	34.127456137525805	29.145079852425987
49	20.53118055630106	17.173335050974583	38.148454301796725	24.147030090927636
50	19.73541167924481	16.56600573262767	38.22395894837414	25.474623639753375
51	18.092344687657675	16.4949383259439	36.87153116982104	28.541185816577386
52	16.82114702651251	17.02382869391275	42.47683868128093	23.678185598293812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	243.0
1	1355.0
2	2467.0
3	35916.0
4	69365.0
5	40231.5
6	11098.0
7	10777.5
8	10457.0
9	10688.5
10	10920.0
11	11108.0
12	11296.0
13	10881.5
14	10111.5
15	9756.0
16	9141.5
17	8527.0
18	7706.0
19	6885.0
20	6866.0
21	6847.0
22	6701.5
23	6556.0
24	7006.5
25	7457.0
26	8356.5
27	9256.0
28	9604.0
29	9952.0
30	12361.0
31	14770.0
32	16160.5
33	17551.0
34	20744.5
35	23938.0
36	25631.5
37	27325.0
38	32649.0
39	41866.5
40	45760.0
41	52887.5
42	60015.0
43	74108.0
44	88201.0
45	108548.5
46	128896.0
47	156777.0
48	184658.0
49	189709.5
50	194761.0
51	169605.0
52	144449.0
53	115674.5
54	86900.0
55	69502.5
56	52105.0
57	42428.0
58	32751.0
59	27328.0
60	21905.0
61	18504.0
62	15103.0
63	12184.5
64	7536.5
65	5807.0
66	5015.0
67	4223.0
68	3452.0
69	2681.0
70	2289.0
71	1897.0
72	1975.0
73	2053.0
74	1649.0
75	1245.0
76	928.5
77	612.0
78	567.0
79	522.0
80	358.0
81	194.0
82	146.0
83	98.0
84	62.5
85	27.0
86	24.0
87	21.0
88	13.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1397265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.10927449291837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57879147167947	24.030311292001183
2	12.214814330936473	8.088472811260175
3	4.814023174643625	4.781664441136383
4	2.448042663671084	3.242116660874438
5	1.435202507605476	2.3759256888617237
6	0.8789889186755331	1.7461611230797032
7	0.6572835703356992	1.5233527504951057
8	0.4750301676967317	1.2582323371750506
9	0.37145329604594435	1.1068694226075997
>10	3.612606990133551	25.970326852121232
>50	0.35984920519942437	7.925145380902536
>100	0.14217452309740788	8.56331857163613
>500	0.006956551276781752	1.5083497876858547
>1k	0.003913060093189736	2.2823903305744633
>5k	6.521766821982893E-4	1.5338296418966197
>10k+	2.1739222739942975E-4	4.063532907691955
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56456	4.040464765094668	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	8542	0.6113371479282741	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	7512	0.5376217109853894	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	5256	0.3761634335648571	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	4081	0.2920705807416632	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	2923	0.2091943904699538	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	2898	0.20740518083541779	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2126	0.15215438732094486	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1997	0.14292206560673887	No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1928	0.1379838470154194	No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1692	0.1210937080653992	No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1418	0.10148397047088419	No Hit
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1400	0.10019573953401824	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5518638196762962	0.0
2	0.0	0.0	0.0	1.878813253033605	0.0
3	0.0	0.0	0.0	3.1455736742851212	0.0
4	0.0	0.0	0.0	3.940770004258319	0.0
5	0.0	0.0	0.0	7.730101305049508	0.0
6	0.0	0.0	0.0	8.707653880974618	0.0
7	0.0	0.0	0.0	12.04188181912522	0.0
8	0.0	0.0	0.0	16.03081734674525	0.0
9	0.0	0.0	0.0	19.250034889587873	0.0
10	0.0	0.0	0.0	21.121261893771045	0.0
11	7.15683853814416E-5	0.0	0.0	22.9231391325196	0.0
12	7.15683853814416E-5	0.0	0.0	24.251663070355303	0.0
13	7.15683853814416E-5	0.0	0.0	24.825713089499843	0.0
14	7.15683853814416E-5	0.0	0.0	25.23651562158932	0.0
15	7.15683853814416E-5	0.0	0.0	26.47128497457533	0.0
16	7.15683853814416E-5	0.0	0.0	28.046290431664715	0.0
17	7.15683853814416E-5	0.0	0.0	29.69658583017538	0.0
18	7.15683853814416E-5	0.0	0.0	30.73225193503022	0.0
19	7.15683853814416E-5	0.0	0.0	31.751672016403475	0.0
20	1.431367707628832E-4	0.0	0.0	32.915803373018	0.0
21	1.431367707628832E-4	0.0	0.0	34.05853578240348	0.0
22	2.862735415257664E-4	0.0	0.0	35.23655140578201	0.0
23	5.725470830515328E-4	0.0	0.0	36.15792279918269	0.0
24	0.0010019573953401824	0.0	0.0	36.920698650578096	0.0
25	0.0010019573953401824	0.0	0.0	37.6726676757809	0.0
26	0.0010019573953401824	0.0	0.0	38.403738732452325	0.0
27	0.0010019573953401824	0.0	0.0	39.14210976443266	0.0
28	0.0010019573953401824	0.0	0.0	39.89622584119691	0.0
29	0.0010019573953401824	0.0	0.0	40.7207652091765	0.0
30	0.0010019573953401824	0.0	0.0	41.49098417265157	0.0
31	0.0010019573953401824	0.0	0.0	42.164800521017845	0.0
32	0.0010019573953401824	0.0	0.0	42.85414720901189	0.0
33	0.0010019573953401824	0.0	0.0	43.55680561668689	0.0
34	0.0010019573953401824	0.0	0.0	44.26991300862757	0.0
35	0.0010019573953401824	0.0	0.0	44.918537285339575	0.0
36	0.0010019573953401824	0.0	0.0	45.54876848700855	0.0
37	0.0010019573953401824	0.0	0.0	46.171985986910144	0.0
38	0.0010019573953401824	0.0	0.0	46.802145620193734	0.0
39	0.0010019573953401824	0.0	0.0	47.43767288238094	0.0
40	0.0010019573953401824	0.0	0.0	48.06432566478084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGACG	70	0.0	46.000004	36
CCGTAGG	35	1.01978E-7	46.000004	1
AGTACGG	55	1.8189894E-12	46.000004	1
GCGGTTT	20	6.311925E-4	46.0	18
TCTTCGA	20	6.311925E-4	46.0	31
GGCAACG	20	6.311925E-4	46.0	8
TCGGGTA	30	1.8616938E-6	46.0	4
ACCGAAC	25	3.4175362E-5	46.0	10
TCGTCCA	20	6.311925E-4	46.0	20
CGTACAA	20	6.311925E-4	46.0	16
CCAACGT	20	6.311925E-4	46.0	46
CATCCGG	45	3.110472E-10	46.0	1
CGTTTTT	16190	0.0	45.488575	1
TCTACGG	150	0.0	44.46667	1
TTACCGG	125	0.0	44.16	1
CGAGGGT	120	0.0	44.083332	3
CCCGCAC	110	0.0	43.909092	32
TACGGGA	630	0.0	43.079365	3
ATTACGG	155	0.0	43.03226	1
ACGGGAC	310	0.0	43.03226	4
>>END_MODULE