FastQCFastQC Report
Thu 26 May 2016
SRR1043247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043247_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1372248
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524013.8186246217884814No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC80820.5889605960438637No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT74880.5456739598090141No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC52200.3803977123668608No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG41080.299362797395223No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC28680.20900012242684993No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC27180.1980691536806758No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG20210.1472765855734532No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG20180.1470579661985297No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC19410.14144673557549364No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG16690.12162524558243115No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT14560.10610326996286386Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG14410.10501017308824645No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC14390.10486442683829746No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG253.8912018E-545.00000417
GTACGCC253.8912018E-545.00000421
AATCCGG406.8175723E-945.01
GGCGTAC207.033766E-445.08
GCACCGT207.033766E-445.030
GCGATCG207.033766E-445.08
TTACCGG950.045.01
TGTCGTG207.033766E-445.033
AGGTCGC207.033766E-445.033
CGTAGTG302.1658107E-644.99999620
CGTAGCG302.1658107E-644.99999627
CGATAGT551.8189894E-1244.9999969
ACGAAGG1200.044.9999961
CGTTTTT152000.044.4227031
GGCGATT3450.043.6956527
TACGCGG1500.043.51
GCGATTG1450.043.4482738
TACGGGT1200.043.1249963
ACGTAGG1000.042.7500041
TACGGGA6450.042.558143