##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043247_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1372248
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2152839719934	33.0	31.0	34.0	31.0	34.0
2	32.50917399770304	34.0	31.0	34.0	31.0	34.0
3	32.595655450035274	34.0	31.0	34.0	31.0	34.0
4	36.147157802379745	37.0	35.0	37.0	35.0	37.0
5	36.09917813689654	37.0	35.0	37.0	35.0	37.0
6	36.329706437903354	37.0	37.0	37.0	35.0	37.0
7	36.2694221452682	37.0	37.0	37.0	35.0	37.0
8	36.16460727215489	37.0	37.0	37.0	35.0	37.0
9	37.713297450606596	39.0	38.0	39.0	35.0	39.0
10	37.46265762456932	39.0	37.0	39.0	35.0	39.0
11	37.31164774880342	39.0	37.0	39.0	34.0	39.0
12	37.36890343436463	39.0	37.0	39.0	34.0	39.0
13	37.39312354618116	39.0	37.0	39.0	35.0	39.0
14	38.79745570771464	40.0	38.0	41.0	35.0	41.0
15	38.87162233065743	40.0	38.0	41.0	35.0	41.0
16	38.91143364756225	41.0	38.0	41.0	35.0	41.0
17	38.86352831266652	40.0	38.0	41.0	35.0	41.0
18	38.65874608671319	40.0	38.0	41.0	35.0	41.0
19	38.430960001399164	40.0	37.0	41.0	35.0	41.0
20	38.17660218852569	40.0	35.0	41.0	34.0	41.0
21	38.10507503016947	40.0	35.0	41.0	34.0	41.0
22	38.06565577067702	40.0	35.0	41.0	34.0	41.0
23	38.018382245774816	40.0	35.0	41.0	34.0	41.0
24	37.951014685392146	40.0	35.0	41.0	34.0	41.0
25	37.9268616168506	40.0	35.0	41.0	34.0	41.0
26	37.88701677830829	40.0	35.0	41.0	34.0	41.0
27	37.88176918457888	40.0	35.0	41.0	34.0	41.0
28	37.84206644863028	40.0	35.0	41.0	34.0	41.0
29	37.81641656610176	40.0	35.0	41.0	34.0	41.0
30	37.75525269484816	40.0	35.0	41.0	34.0	41.0
31	37.57263045746833	40.0	35.0	41.0	33.0	41.0
32	37.33445922311419	40.0	35.0	41.0	33.0	41.0
33	37.05712451393626	40.0	35.0	41.0	32.0	41.0
34	36.75057788388105	40.0	35.0	41.0	31.0	41.0
35	36.53860162303024	40.0	35.0	41.0	30.0	41.0
36	36.493421743008554	40.0	35.0	41.0	30.0	41.0
37	36.33701269741329	40.0	35.0	41.0	30.0	41.0
38	36.27087596411145	40.0	35.0	41.0	30.0	41.0
39	36.17095233514642	40.0	35.0	41.0	29.0	41.0
40	36.10490523578828	39.0	35.0	41.0	29.0	41.0
41	36.023444741766795	39.0	35.0	41.0	29.0	41.0
42	35.96260296972559	39.0	35.0	41.0	28.0	41.0
43	35.85194148579557	39.0	35.0	41.0	27.0	41.0
44	35.76106942768362	39.0	35.0	41.0	27.0	41.0
45	35.716666375174164	39.0	35.0	41.0	27.0	41.0
46	35.6208469606077	39.0	35.0	41.0	26.0	41.0
47	35.466645241967925	39.0	35.0	40.0	26.0	41.0
48	35.43096655998041	39.0	35.0	40.0	25.0	41.0
49	35.36240096542316	39.0	35.0	40.0	26.0	41.0
50	35.245867365082695	38.0	35.0	40.0	25.0	41.0
51	34.8044325806997	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	4.0
15	13.0
16	36.0
17	92.0
18	194.0
19	451.0
20	759.0
21	1327.0
22	2171.0
23	3842.0
24	6868.0
25	13190.0
26	21298.0
27	24611.0
28	23142.0
29	21506.0
30	21958.0
31	24678.0
32	29706.0
33	38158.0
34	67606.0
35	107313.0
36	106110.0
37	107759.0
38	194276.0
39	555093.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.62649317033073	18.91706163900403	26.250284205187402	27.206160985477844
2	32.383650768665724	20.25851012353452	26.142213360850224	21.21562574694953
3	21.657236884294967	22.56042639522885	35.74404918061458	20.0382875398616
4	23.247474217488385	24.597157365140994	32.852443581626645	19.302924835743976
5	21.431330196874036	30.32782704001026	29.374865184718796	18.865977578396908
6	76.76790201188123	1.577848901947753	18.32008499921297	3.334164086958043
7	77.31335735231532	1.2543651001859721	15.561545726428458	5.870731821070244
8	66.60436014481348	3.5159096606444318	22.20196349347931	7.677766701062781
9	34.235065381767726	25.509820382321564	24.926179524400837	15.328934711509874
10	23.102748191289038	20.08288589234599	44.37076971509523	12.443596201269742
11	21.816901901114083	17.258615060834483	41.03842745626155	19.88605558178988
12	19.10405407768858	19.557179168779985	43.077053127423035	18.2617136261084
13	14.90423013915852	23.022369134442172	41.99721916155097	20.076181564848337
14	14.355932746850423	23.028563350065003	42.802977304393956	19.812526598690617
15	15.349995044627502	20.86991564207053	44.30554826824306	19.47454104505891
16	15.715016527624744	24.397703622085803	38.633978697728104	21.253301152561342
17	16.443674904244716	22.21129125347605	40.47118305146009	20.87385079081915
18	17.148576642122997	27.443581626644747	39.430700573074255	15.977141158158
19	17.308606024566988	23.585678390495012	36.891436533337995	22.214279051600002
20	15.882624715066083	28.346844010703602	36.0439949630096	19.726536311220713
21	15.681349143886528	23.28004850435198	37.34601908692889	23.692583264832596
22	14.023631296966728	25.43213763109875	34.781103707201616	25.763127364732906
23	13.861561467023453	23.08270808192105	41.65697454104506	21.398755910010436
24	17.634276020078005	22.603567285213753	38.18150946476147	21.580647229946774
25	14.781657542951422	25.45137613609202	37.965732141711996	21.80123417924457
26	16.020646413767775	22.784802747025317	37.13796631512671	24.056584524080197
27	18.927336749625432	23.483218776780873	37.98562650483003	19.603817968763664
28	15.394374777736969	22.890978890113157	36.406757379132635	25.307888953017237
29	16.89024141408842	25.357296931749946	35.807740291842286	21.944721362319346
30	19.06659729145169	21.698774565530428	34.17203012866479	25.06259801435309
31	18.57951332412217	24.0022940459742	35.643484268149784	21.774708361753852
32	16.699823938530063	23.91236860975567	34.87161212841994	24.516195323294333
33	15.451580180841948	21.816901901114083	38.310859261591204	24.42065865645277
34	19.05348012895628	20.299246200395263	37.98322169170587	22.66405197894258
35	16.837408398481905	20.432531145973616	39.527330336790435	23.202730118754044
36	21.640476065550835	20.967419883286404	37.330642857559276	20.06146119360349
37	19.80108551806962	20.508100576572165	38.345911234703934	21.344902670654285
38	21.060697483253758	20.583815753420666	33.669861424465545	24.68562533886003
39	24.610784639511227	20.175580507313548	34.28170418175141	20.931930671423824
40	19.872136814919752	20.01351067737027	36.449606776617635	23.66474573109234
41	20.760751700858737	22.655817315820464	31.500355620849874	25.083075362470925
42	20.202179197929237	18.823346800286828	36.330532090409314	24.64394191137462
43	22.03326220916336	21.295567565046554	35.541024654435645	21.13014557135445
44	17.437372836396918	23.0254298056911	36.63113373092911	22.906063626982878
45	19.879278381167254	20.729926368994526	35.388574076989	24.00222117284922
46	23.054724801930846	19.06462971707738	34.253065043636425	23.62758043735535
47	18.62360156473174	18.12325468865686	37.49766806000082	25.755475686610584
48	19.711378701226018	16.933528050323265	34.0448665255843	29.31022672286642
49	20.701870215879346	16.788291912249097	38.11264436166057	24.397193510210982
50	19.890063603663478	16.252382951186668	38.24971870973759	25.60783473541226
51	18.381006931691648	16.246407354938757	36.62180597093237	28.750779742437228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	1242.0
2	2343.0
3	33536.0
4	64729.0
5	38083.5
6	11438.0
7	10913.5
8	10389.0
9	10454.0
10	10519.0
11	10517.0
12	10515.0
13	10327.5
14	10140.0
15	9489.5
16	8839.0
17	8141.5
18	7444.0
19	6976.0
20	6508.0
21	6215.5
22	5923.0
23	6165.5
24	6408.0
25	6580.0
26	7939.0
27	9126.0
28	10064.0
29	11002.0
30	13200.0
31	15398.0
32	16735.5
33	18073.0
34	21857.0
35	25641.0
36	28401.5
37	31162.0
38	35636.5
39	40111.0
40	46190.0
41	52269.0
42	61348.0
43	70427.0
44	81897.0
45	93367.0
46	118688.0
47	144009.0
48	174023.5
49	204038.0
50	197570.5
51	191103.0
52	154762.0
53	118421.0
54	92742.0
55	67063.0
56	53802.5
57	40542.0
58	33394.0
59	26246.0
60	22061.5
61	17877.0
62	14586.0
63	11295.0
64	9371.0
65	7447.0
66	6175.5
67	4904.0
68	4043.0
69	3182.0
70	2646.5
71	2111.0
72	2141.0
73	2171.0
74	1721.0
75	1072.5
76	874.0
77	702.5
78	531.0
79	427.5
80	324.0
81	212.5
82	101.0
83	70.0
84	39.0
85	31.5
86	24.0
87	13.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1372248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.56086702469209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.98428819119216	23.438708356605208
2	12.304466461569573	8.012881925299009
3	4.91285517274904	4.799004719743565
4	2.571024733657817	3.348591778793063
5	1.4427100885846988	2.3487945674794055
6	0.9248961942847205	1.806925319624914
7	0.6763104755722469	1.5414878823759939
8	0.4834409736332056	1.2593005805406783
9	0.40377804718487714	1.1832626971688969
>10	3.775545043150604	26.45682345825876
>50	0.35932612915994366	7.751129215256787
>100	0.149171974241186	8.833580737511749
>500	0.007221638530365939	1.5357030080124217
>1k	0.004062171673330841	2.305575270558292
>5k	6.770286122218069E-4	1.527693455982499
>10k+	2.256762040739356E-4	3.8505370267887895
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52401	3.8186246217884814	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	8082	0.5889605960438637	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	7488	0.5456739598090141	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	5220	0.3803977123668608	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	4108	0.299362797395223	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2868	0.20900012242684993	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	2718	0.1980691536806758	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	2021	0.1472765855734532	No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	2018	0.1470579661985297	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1941	0.14144673557549364	No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1669	0.12162524558243115	No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	1456	0.10610326996286386	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1441	0.10501017308824645	No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1439	0.10486442683829746	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5589368685543721	0.0
2	0.0	0.0	0.0	1.8715275955949653	0.0
3	0.0	0.0	0.0	3.1655356757670625	0.0
4	0.0	0.0	0.0	3.996507919851222	0.0
5	0.0	0.0	0.0	7.848727052252945	0.0
6	0.0	0.0	0.0	8.848619200027983	0.0
7	0.0	0.0	0.0	12.258279844459603	0.0
8	0.0	0.0	0.0	16.353749468026187	0.0
9	0.0	0.0	0.0	19.664594154992393	0.0
10	0.0	0.0	0.0	21.636468043677237	0.0
11	0.0	0.0	0.0	23.48059534428179	0.0
12	0.0	0.0	0.0	24.819128903813304	0.0
13	0.0	0.0	0.0	25.436582891722196	0.0
14	0.0	0.0	0.0	25.867408806571408	0.0
15	0.0	0.0	0.0	27.15099602987215	0.0
16	0.0	0.0	0.0	28.752820189936514	0.0
17	0.0	0.0	0.0	30.472553066209606	0.0
18	0.0	0.0	0.0	31.529869236464545	0.0
19	0.0	0.0	0.0	32.57632731109828	0.0
20	1.4574624994898882E-4	0.0	0.0	33.776547679428205	0.0
21	1.4574624994898882E-4	0.0	0.0	34.95308428214142	0.0
22	2.186193749234832E-4	0.0	0.0	36.157239799219965	0.0
23	3.6436562487247203E-4	0.0	0.0	37.0965743801412	0.0
24	0.0010202237496429217	0.0	0.0	37.86764491549632	0.0
25	0.0010202237496429217	0.0	0.0	38.622391870857165	0.0
26	0.0010202237496429217	0.0	0.0	39.363292932472845	0.0
27	0.0010202237496429217	0.0	0.0	40.09646944284123	0.0
28	0.0010202237496429217	0.0	0.0	40.87118363444509	0.0
29	0.0010202237496429217	0.0	0.0	41.72110289102261	0.0
30	0.0010202237496429217	0.0	0.0	42.50157405949945	0.0
31	0.0010202237496429217	0.0	0.0	43.19219266488273	0.0
32	0.0010202237496429217	0.0	0.0	43.8957098133865	0.0
33	0.0010202237496429217	0.0	0.0	44.59602054439139	0.0
34	0.0010202237496429217	0.0	0.0	45.30901119914185	0.0
35	0.0010202237496429217	0.0	0.0	45.97142790516	0.0
36	0.0010202237496429217	0.0	0.0	46.6062985699378	0.0
37	0.0010202237496429217	0.0	0.0	47.248893785962885	0.0
38	0.0010202237496429217	0.0	0.0	47.882744226991036	0.0
39	0.0010202237496429217	0.0	0.0	48.52176865989238	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	25	3.8912018E-5	45.000004	17
GTACGCC	25	3.8912018E-5	45.000004	21
AATCCGG	40	6.8175723E-9	45.0	1
GGCGTAC	20	7.033766E-4	45.0	8
GCACCGT	20	7.033766E-4	45.0	30
GCGATCG	20	7.033766E-4	45.0	8
TTACCGG	95	0.0	45.0	1
TGTCGTG	20	7.033766E-4	45.0	33
AGGTCGC	20	7.033766E-4	45.0	33
CGTAGTG	30	2.1658107E-6	44.999996	20
CGTAGCG	30	2.1658107E-6	44.999996	27
CGATAGT	55	1.8189894E-12	44.999996	9
ACGAAGG	120	0.0	44.999996	1
CGTTTTT	15200	0.0	44.422703	1
GGCGATT	345	0.0	43.695652	7
TACGCGG	150	0.0	43.5	1
GCGATTG	145	0.0	43.448273	8
TACGGGT	120	0.0	43.124996	3
ACGTAGG	100	0.0	42.750004	1
TACGGGA	645	0.0	42.55814	3
>>END_MODULE