FastQCFastQC Report
Thu 26 May 2016
SRR1043246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043246_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2535949
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1215154.79169730937018No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC130090.5129835024284795No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT106710.42078921934155616No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA101770.4013093323249009No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTT91850.36219182641291287Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC60700.2393581258929103No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT60380.23809627086349133No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC43310.17078419163792333No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT42580.1679055848520613No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC40420.15938806340348327No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG37970.1497269858344943No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC35310.13923781590244913No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT32510.12819658439503318No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC29930.11802287822034277No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT29880.11782571337199604No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT27620.10891386222672458No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG25920.10221025738293633No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA253.4183307E-546.044
TCTATCG301.8622995E-646.020
ACTACGG1900.045.9999961
CGTTTTT285050.045.2576751
CGACGGT4000.043.69999727
CTAGCGG5450.042.623851
TAATACG554.7293724E-1141.8181816
CTTGCGG8100.041.740741
ATTGCGG4800.041.68751
CGGTCTA4200.041.61904530
TACGGGA4200.041.0714263
ACGGGAT5850.040.888894
ACGATGG1350.040.888891
GTTTTTT322800.040.8271372
GCGTAGG3500.040.742861
TAGCGGA850.040.58823445
GCGATAG2500.040.488
AGGGTAC8200.040.3902445
AGGGCGA11300.040.3008845
TACACGG1600.040.251
Produced by FastQC (version 0.11.5)