##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043246_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2535949
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38547384036509	33.0	31.0	34.0	31.0	34.0
2	32.71354905007948	34.0	31.0	34.0	31.0	34.0
3	32.82426854798736	34.0	31.0	34.0	31.0	34.0
4	36.24715165801836	37.0	37.0	37.0	35.0	37.0
5	36.183051788502056	37.0	37.0	37.0	35.0	37.0
6	36.34349744415207	37.0	37.0	37.0	35.0	37.0
7	36.285835795593684	37.0	37.0	37.0	35.0	37.0
8	36.269880033076376	37.0	37.0	37.0	35.0	37.0
9	37.872469832792376	39.0	38.0	39.0	35.0	39.0
10	37.66910651594334	39.0	37.0	39.0	35.0	39.0
11	37.2937760183663	39.0	37.0	39.0	35.0	39.0
12	37.29413840735756	39.0	37.0	39.0	35.0	39.0
13	37.28260623537776	39.0	37.0	39.0	35.0	39.0
14	38.593771404708846	40.0	37.0	41.0	35.0	41.0
15	38.63376589986628	40.0	38.0	41.0	35.0	41.0
16	38.47403437529698	40.0	37.0	41.0	34.0	41.0
17	38.445419840856424	40.0	37.0	41.0	34.0	41.0
18	38.2710693314416	40.0	37.0	41.0	34.0	41.0
19	38.07846451170745	40.0	37.0	41.0	34.0	41.0
20	37.8310853254541	40.0	35.0	41.0	34.0	41.0
21	37.761332345406	40.0	35.0	41.0	34.0	41.0
22	37.71548087126358	39.0	35.0	41.0	34.0	41.0
23	37.73537401580237	39.0	35.0	41.0	34.0	41.0
24	37.66242341624378	39.0	35.0	41.0	34.0	41.0
25	37.564398574261546	39.0	35.0	41.0	34.0	41.0
26	37.49331078819014	39.0	35.0	41.0	34.0	41.0
27	37.51757152844951	39.0	35.0	41.0	34.0	41.0
28	37.49912754554607	39.0	35.0	41.0	34.0	41.0
29	37.37917126882284	39.0	35.0	41.0	33.0	41.0
30	37.32850069145712	39.0	35.0	41.0	33.0	41.0
31	37.2377618792807	39.0	35.0	41.0	33.0	41.0
32	36.99925708285143	39.0	35.0	41.0	33.0	41.0
33	36.66189856341748	39.0	35.0	41.0	31.0	41.0
34	36.41745910505298	39.0	35.0	41.0	31.0	41.0
35	36.268611080112414	39.0	35.0	41.0	31.0	41.0
36	36.24466343763223	39.0	35.0	41.0	31.0	41.0
37	36.17946338826214	39.0	35.0	41.0	31.0	41.0
38	36.09831704028748	39.0	35.0	41.0	31.0	41.0
39	36.07403342890571	39.0	35.0	41.0	31.0	41.0
40	36.003816322804596	38.0	35.0	41.0	30.0	41.0
41	35.959761414760315	38.0	35.0	41.0	30.0	41.0
42	35.88662508591458	38.0	35.0	41.0	30.0	41.0
43	35.788204731246566	38.0	35.0	41.0	30.0	41.0
44	35.62979066219392	38.0	35.0	40.0	30.0	41.0
45	35.547450678227364	38.0	35.0	40.0	29.0	41.0
46	35.533318690557266	38.0	35.0	40.0	29.0	41.0
47	35.46478221762346	37.0	35.0	40.0	29.0	41.0
48	35.38413548537451	37.0	35.0	40.0	29.0	41.0
49	35.34262794717086	37.0	35.0	40.0	29.0	41.0
50	35.25376062373494	37.0	35.0	40.0	29.0	41.0
51	35.138276045772216	37.0	35.0	40.0	28.0	41.0
52	34.797109484457295	36.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	9.0
14	20.0
15	50.0
16	87.0
17	170.0
18	404.0
19	851.0
20	1523.0
21	2578.0
22	4259.0
23	6757.0
24	11813.0
25	20398.0
26	31094.0
27	37651.0
28	40432.0
29	41068.0
30	43350.0
31	49440.0
32	59100.0
33	76740.0
34	187067.0
35	248132.0
36	229590.0
37	214500.0
38	336889.0
39	888866.0
40	3108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.05803705042964	17.029049085766314	23.86207293600936	30.05084092779468
2	35.17661435620354	19.190449019282326	24.21491914861064	21.418017475903497
3	25.58580633916534	20.545839052756975	34.16649940515366	19.701855202924033
4	22.96757545202999	23.07309807886515	32.30479004112464	21.654536427980215
5	21.3434102972891	31.06174453823795	29.11028573524152	18.484559429231425
6	77.32616073903694	1.8943992958848939	17.732730429515737	3.0467095355624267
7	77.00884363210776	1.8196738183614891	16.108644140714187	5.062838408816582
8	67.22852076283868	3.373924317878632	21.803277589572975	7.594277329709706
9	40.332080810773405	16.65845803681383	25.83387915135517	17.17558200105759
10	29.012886300158247	15.726341499769909	36.951807784778005	18.30896441529384
11	26.53965044249707	13.036303174866687	39.13414662518844	21.289899757447802
12	21.178186154374558	14.342362563284988	42.20861697139809	22.270834310942373
13	16.54828231955769	17.335522126036444	44.066461904399496	22.049733650006367
14	17.62326450571364	17.747557226111407	43.2108453285141	21.418332939660853
15	21.54396638102738	14.595403929653159	42.92613928750145	20.934490401818017
16	19.591166857062188	18.748287130379985	40.144892503753034	21.515653508804792
17	20.55755064474877	19.855801516513147	39.35232924636891	20.234318592369167
18	20.100167629554065	26.558933164665376	35.594840432516584	17.74605877326397
19	21.05779729797405	22.935437581749476	33.85186374016197	22.154901380114506
20	20.6920565042909	27.0381620450569	33.984437384190294	18.285344066461903
21	18.011363793199312	20.870924454711037	36.756851182732774	24.360860569356877
22	19.565732591625462	22.386096881285862	34.22028597578264	23.827884551306038
23	21.14979441621263	19.93253807548969	37.23880093803148	21.6788665702662
24	23.18362869284832	18.46791871603096	33.6293040593482	24.719148531772525
25	17.37617751776554	19.315017770467783	37.84547717639432	25.46332753537236
26	16.945569488976318	18.26921598186714	37.86681041298544	26.918404116171107
27	20.602346498293144	21.08926480777019	37.33328233335923	20.975106360577442
28	22.53673082542275	21.75860003493761	33.13994090575165	22.564728233887983
29	21.77539848001675	19.24774512421188	37.93676450117885	21.040091894592518
30	25.246288470312294	19.038434921207013	34.586933727768184	21.128342880712506
31	27.02156076482611	18.295478339666925	32.81560473022131	21.86735616528566
32	23.775202103827798	17.8992164274597	36.29118724390751	22.03439422480499
33	21.635490303629922	20.626794939488136	38.06894381551049	19.668770941371456
34	21.330555149176895	20.642094931719843	36.69785946010744	21.329490458995824
35	21.584464040877794	26.95704842644706	33.69212078003146	17.766366752643684
36	24.04622490436519	24.09125735572758	31.144869238300927	20.717648501606302
37	20.977630070636277	28.093033416681486	30.84963459438656	20.079701918295676
38	22.314683773214682	24.06381200883772	28.521748662926584	25.09975555502102
39	24.345600010094838	21.255119878199444	32.660593726451125	21.738686385254592
40	20.145831008431163	20.54611508354466	35.77390554778507	23.534148360239104
41	20.8689133732579	22.78744564658043	31.112652502081072	25.23098847808059
42	20.389723925836048	17.49325400471382	34.764697555037586	27.35232451441255
43	22.73728690916103	18.94064115642704	34.47561445439163	23.846457480020298
44	21.57559162270219	19.877726247649303	35.1451468464074	23.401535283241106
45	21.8508337509942	21.552365603566948	33.67339011943852	22.923410526000325
46	22.597773062470893	19.54195451091485	33.22215864751223	24.638113779102024
47	19.34743167153598	20.478250942743724	36.2566045295075	23.917712856212802
48	21.712108563697456	18.56527871814457	32.803972004168855	26.918640713989124
49	21.11126840484568	16.751086082567117	36.42242016696708	25.71522534562012
50	19.7892386637113	17.0519596411442	38.376718143779705	24.782083551364796
51	18.298435812392125	15.913687538668956	36.9973528647461	28.79052378419282
52	16.448122576597555	17.033031815702916	41.1249200989452	25.393925508754318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	781.0
1	3884.0
2	6987.0
3	85232.5
4	163478.0
5	91665.0
6	19852.0
7	17768.0
8	15684.0
9	15371.5
10	15059.0
11	14654.0
12	14249.0
13	13817.0
14	12344.5
15	11304.0
16	10697.0
17	10090.0
18	9318.0
19	8546.0
20	8108.5
21	7671.0
22	7548.0
23	7425.0
24	8385.0
25	9345.0
26	9703.0
27	10061.0
28	10987.5
29	11914.0
30	13012.5
31	14111.0
32	16277.5
33	18444.0
34	21890.5
35	25337.0
36	28352.0
37	31367.0
38	34346.5
39	43637.0
40	49948.0
41	63698.5
42	77449.0
43	106504.0
44	135559.0
45	178557.0
46	221555.0
47	244320.0
48	267085.0
49	256958.0
50	246831.0
51	219913.5
52	192996.0
53	165849.0
54	138702.0
55	127128.5
56	115555.0
57	111977.5
58	108400.0
59	107558.0
60	106716.0
61	107512.5
62	108309.0
63	108325.0
64	91021.5
65	73702.0
66	58977.0
67	44252.0
68	35514.5
69	26777.0
70	23354.5
71	19932.0
72	18841.5
73	17751.0
74	14549.0
75	11347.0
76	8584.5
77	5822.0
78	4567.5
79	3313.0
80	2420.0
81	1527.0
82	1180.5
83	834.0
84	655.0
85	476.0
86	346.0
87	216.0
88	146.0
89	55.0
90	34.0
91	28.0
92	22.0
93	13.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2535949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.64146642581013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75419479497486	20.11032632762148
2	11.110364722166317	6.142135468925311
3	4.29170014854597	3.558866566970334
4	2.1349091796751725	2.360480816485805
5	1.3060912417012702	1.8051138603265162
6	0.8877244985116843	1.4722804152587916
7	0.639935162287903	1.2382122412153502
8	0.5239013790566677	1.1585121903704405
9	0.43937280723304417	1.0930417829591523
>10	5.276518564056749	32.24837737660407
>50	0.4399179418792475	8.0504134176789
>100	0.1810368435328753	9.205351559361448
>500	0.007882994007987764	1.4654598273644024
>1k	0.005446432223700637	3.0923619038910095
>5k	4.2998149134478714E-4	0.8435803218186056
>10k+	5.733086551263829E-4	6.155485923148471
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121515	4.79169730937018	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13009	0.5129835024284795	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	10671	0.42078921934155616	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	10177	0.4013093323249009	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTT	9185	0.36219182641291287	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	6070	0.2393581258929103	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	6038	0.23809627086349133	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4331	0.17078419163792333	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	4258	0.1679055848520613	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4042	0.15938806340348327	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	3797	0.1497269858344943	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	3531	0.13923781590244913	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	3251	0.12819658439503318	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2993	0.11802287822034277	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT	2988	0.11782571337199604	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	2762	0.10891386222672458	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG	2592	0.10221025738293633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3716163061638858	0.0
2	0.0	0.0	0.0	1.2164282483598843	0.0
3	0.0	0.0	0.0	2.0161288732541545	0.0
4	0.0	0.0	0.0	2.5705169938354437	0.0
5	0.0	0.0	0.0	5.2240403888248546	0.0
6	0.0	0.0	0.0	6.232814618905979	0.0
7	0.0	0.0	0.0	9.030899280703201	0.0
8	0.0	0.0	0.0	12.377693715449325	0.0
9	0.0	0.0	0.0	15.545856797593327	0.0
10	0.0	0.0	0.0	17.287413903039848	0.0
11	0.0	0.0	0.0	18.656999805595458	0.0
12	0.0	0.0	0.0	19.632689774124007	0.0
13	0.0	0.0	0.0	20.137352919952253	0.0
14	0.0	0.0	0.0	20.47482027438249	0.0
15	0.0	0.0	0.0	21.257919619045968	0.0
16	0.0	0.0	0.0	22.12213258231928	0.0
17	0.0	0.0	0.0	23.21541166640181	0.0
18	0.0	0.0	0.0	23.85611855758929	0.0
19	3.9432969669342716E-5	0.0	0.0	24.555856604371776	0.0
20	3.9432969669342716E-5	0.0	0.0	25.283118863983464	0.0
21	3.9432969669342716E-5	0.0	0.0	26.016138337166876	0.0
22	3.9432969669342716E-5	0.0	0.0	26.735435136905355	0.0
23	3.9432969669342716E-5	0.0	0.0	27.386749496933888	0.0
24	7.886593933868543E-5	0.0	0.0	27.9331327246723	0.0
25	7.886593933868543E-5	0.0	0.0	28.453805656186304	0.0
26	1.1829890900802816E-4	0.0	0.0	28.95318478407886	0.0
27	1.1829890900802816E-4	0.0	0.0	29.507651770599487	0.0
28	1.5773187867737087E-4	0.0	0.0	30.030611814354312	0.0
29	1.5773187867737087E-4	0.0	0.0	30.5869321504494	0.0
30	1.5773187867737087E-4	0.0	0.0	31.074836284168175	0.0
31	1.5773187867737087E-4	0.0	0.0	31.584744014962446	0.0
32	1.5773187867737087E-4	0.0	0.0	32.17158546958161	0.0
33	1.5773187867737087E-4	0.0	0.0	32.71627307962424	0.0
34	1.5773187867737087E-4	0.0	0.0	33.30532278054488	0.0
35	1.971648483467136E-4	0.0	0.0	33.8204356633355	0.0
36	1.971648483467136E-4	0.0	0.0	34.347930498602295	0.0
37	1.971648483467136E-4	0.0	0.0	35.08272445542083	0.0
38	1.971648483467136E-4	0.0	0.0	35.77646869081358	0.0
39	1.971648483467136E-4	0.0	0.0	36.615168522710825	0.0
40	1.971648483467136E-4	0.0	0.0	37.371926643635184	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTCGA	25	3.4183307E-5	46.0	44
TCTATCG	30	1.8622995E-6	46.0	20
ACTACGG	190	0.0	45.999996	1
CGTTTTT	28505	0.0	45.257675	1
CGACGGT	400	0.0	43.699997	27
CTAGCGG	545	0.0	42.62385	1
TAATACG	55	4.7293724E-11	41.81818	16
CTTGCGG	810	0.0	41.74074	1
ATTGCGG	480	0.0	41.6875	1
CGGTCTA	420	0.0	41.619045	30
TACGGGA	420	0.0	41.071426	3
ACGGGAT	585	0.0	40.88889	4
ACGATGG	135	0.0	40.88889	1
GTTTTTT	32280	0.0	40.827137	2
GCGTAGG	350	0.0	40.74286	1
TAGCGGA	85	0.0	40.588234	45
GCGATAG	250	0.0	40.48	8
AGGGTAC	820	0.0	40.390244	5
AGGGCGA	1130	0.0	40.300884	5
TACACGG	160	0.0	40.25	1
>>END_MODULE