FastQCFastQC Report
Thu 26 May 2016
SRR1043245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043245_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2497200
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1153714.620014416146083No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC122750.4915505366009931No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC103000.4124619573922793No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT90020.3604837417908057No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG85230.3413022585295531No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC61180.24499439372096748No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG60400.2418708954028512No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC42230.16910940253083453No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC41180.16490469325644722No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC39360.15761653051417587No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCC35740.1431202947300977No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT35510.14219926317475573No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC33860.1355918628864328No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT29160.11677078327727054No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC28890.11568957232099952No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC26720.10699983982059907No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT25980.10403652090341182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATCG207.034877E-445.00000410
CGTTTTT277500.044.2864841
ATAGCGG3400.042.352941
ACTACGG1700.042.352941
CGACGGT4100.042.25609627
CACGACG4000.042.187525
ACGTAGG1400.041.7857171
GCGATAG2050.041.7073178
TACGCGG3100.041.3709681
ATACCGG603.6379788E-1241.2500041
TTTACGG1500.040.51
CGCACTT35450.040.23977734
AGGGCGA12150.040.1851845
TAACGGG5550.040.1351362
GTTTTTT311750.040.04172
CACTTAC35650.040.01402736
TCGATCC451.9299478E-840.016
TAACCCG900.040.037
GGGCGAT25650.039.9122776
TACGGGA4400.039.8863643