##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043245_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2497200
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36739788563191	33.0	31.0	34.0	31.0	34.0
2	32.6498790645523	34.0	31.0	34.0	31.0	34.0
3	32.745354396924554	34.0	31.0	34.0	31.0	34.0
4	36.22151129264777	37.0	37.0	37.0	35.0	37.0
5	36.20256206951786	37.0	37.0	37.0	35.0	37.0
6	36.36385351593785	37.0	37.0	37.0	35.0	37.0
7	36.31747276950184	37.0	37.0	37.0	35.0	37.0
8	36.22369854236745	37.0	37.0	37.0	35.0	37.0
9	37.83836056383149	39.0	38.0	39.0	35.0	39.0
10	37.6265517379465	39.0	37.0	39.0	35.0	39.0
11	37.294713679320836	39.0	37.0	39.0	35.0	39.0
12	37.3041718724972	39.0	37.0	39.0	35.0	39.0
13	37.305624699663625	39.0	37.0	39.0	35.0	39.0
14	38.61327767099151	40.0	38.0	41.0	35.0	41.0
15	38.66192095146564	40.0	38.0	41.0	35.0	41.0
16	38.54247677398686	40.0	38.0	41.0	34.0	41.0
17	38.488004565112924	40.0	37.0	41.0	34.0	41.0
18	38.33282276149287	40.0	37.0	41.0	34.0	41.0
19	38.15996195739228	40.0	37.0	41.0	34.0	41.0
20	37.93472809546692	40.0	35.0	41.0	34.0	41.0
21	37.866882908857924	40.0	35.0	41.0	34.0	41.0
22	37.83627222489188	40.0	35.0	41.0	34.0	41.0
23	37.8224135031235	40.0	35.0	41.0	34.0	41.0
24	37.751536520903414	40.0	35.0	41.0	34.0	41.0
25	37.72021584174275	39.0	35.0	41.0	34.0	41.0
26	37.70589380105719	39.0	35.0	41.0	34.0	41.0
27	37.68200504565113	39.0	35.0	41.0	34.0	41.0
28	37.5962586096428	39.0	35.0	41.0	34.0	41.0
29	37.526638234823004	39.0	35.0	41.0	34.0	41.0
30	37.43852034278392	39.0	35.0	41.0	34.0	41.0
31	37.3368444658017	39.0	35.0	41.0	34.0	41.0
32	37.11920871375941	39.0	35.0	41.0	33.0	41.0
33	36.77446179721288	39.0	35.0	41.0	32.0	41.0
34	36.495564231939774	39.0	35.0	41.0	31.0	41.0
35	36.347383869934326	39.0	35.0	41.0	31.0	41.0
36	36.294179881467244	39.0	35.0	41.0	31.0	41.0
37	36.17612926477655	39.0	35.0	41.0	31.0	41.0
38	36.099421752362645	39.0	35.0	41.0	31.0	41.0
39	36.03852074323242	38.0	35.0	41.0	30.0	41.0
40	35.95061068396604	38.0	35.0	41.0	30.0	41.0
41	35.86412862405895	38.0	35.0	41.0	30.0	41.0
42	35.777131987826365	38.0	35.0	41.0	30.0	41.0
43	35.663584814992795	38.0	35.0	40.0	30.0	41.0
44	35.56446099631587	38.0	35.0	40.0	30.0	41.0
45	35.5112934486625	38.0	35.0	40.0	29.0	41.0
46	35.44352394682044	37.0	35.0	40.0	29.0	41.0
47	35.31743272465161	37.0	35.0	40.0	29.0	41.0
48	35.25818596828448	37.0	35.0	40.0	28.0	41.0
49	35.20925076085216	37.0	35.0	40.0	28.0	41.0
50	35.14705430081691	37.0	35.0	40.0	28.0	41.0
51	34.746703908377384	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	11.0
15	32.0
16	79.0
17	186.0
18	364.0
19	687.0
20	1345.0
21	2361.0
22	3708.0
23	5824.0
24	9973.0
25	17578.0
26	28199.0
27	36775.0
28	39640.0
29	40126.0
30	42968.0
31	48568.0
32	56987.0
33	75047.0
34	181456.0
35	248746.0
36	218459.0
37	213201.0
38	352482.0
39	872003.0
40	389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.390237065513375	17.071520102514818	23.96492071119654	29.57332212077527
2	34.8881547333013	19.26381547333013	24.249519461797213	21.59851033157136
3	25.779673234022106	20.622096748358164	33.84586737145603	19.752362646163704
4	23.077566874899887	23.243632868813073	31.946139676437614	21.73266057984943
5	21.533837898446258	31.199583533557586	28.70943456671472	18.557144001281436
6	77.61689091782796	1.8889155854557103	17.420551017139196	3.0736424795771264
7	77.36629024507448	1.7843184366490468	15.742791926958194	5.106599391318277
8	67.44545891398366	3.3517940092904057	21.514456190933846	7.688290885792087
9	40.46740349191094	16.63094666025949	25.51189332051898	17.38975652731059
10	29.29340861765177	15.735263495114529	36.665024827807144	18.306303059426558
11	26.715841742751884	13.009050136152492	38.91574563511133	21.359362485984303
12	21.355077687009448	14.257568476693896	42.044569918308504	22.342783917988147
13	16.649647605317956	17.212357840781674	43.95651129264777	22.181483261252602
14	17.692495595066475	17.74167067115169	43.09490629505046	21.47092743873138
15	21.573081851673876	14.490189011693097	42.87626141278232	21.060467723850714
16	19.650008008970048	18.75668748998879	40.02787121576165	21.565433285279514
17	20.675836937369855	19.831931763575202	39.17139195899407	20.320839340060868
18	20.179280794489827	26.712598109883068	35.4327246516098	17.6753964440173
19	21.05001601794009	23.095747236905336	33.56659458593625	22.287642159218322
20	20.678319718084254	27.169349671632226	33.67847989748518	18.473850712798335
21	18.03932404292808	21.03151529713279	36.48902771103636	24.44013294890277
22	19.616530514175878	22.466442415505366	33.86488867531636	24.052138395002405
23	21.228816274227132	20.013415024827808	36.93977254525068	21.817996155694377
24	23.25192215281115	18.452506807624538	33.438451065193014	24.857119974371294
25	17.266778792247315	19.41017940092904	37.695699183085054	25.627342623738585
26	17.005926637834374	18.146083613647285	37.657776709915105	27.190213038603233
27	20.66566554541086	21.04877462758289	37.15533397405093	21.13022585295531
28	22.617691814832614	21.84146243793048	32.84414544289604	22.69670030434086
29	21.84338459074163	19.20627102354637	37.68765016818837	21.262694217523624
30	25.364968765016823	18.9528271664264	34.35579849431363	21.32640557424315
31	27.233621656255007	18.25864968765017	32.569037321800415	21.93869133429441
32	23.87774307224091	17.770703187570078	36.17571680281916	22.175836937369855
33	21.719125420470927	20.50941053980458	38.00748838699343	19.76397565273106
34	21.53103475893	20.503283677719043	36.548454268781036	21.41722729456992
35	21.667988146724333	27.02747076725933	33.58970046452026	17.714840621496077
36	24.08641678680122	24.102835175396446	30.972729456991832	20.83801858081051
37	21.093224411340703	28.019621976613806	30.714480217843988	20.172673394201507
38	22.431323081851673	24.016017940092905	28.35884190293128	25.193817075124137
39	24.537642159218326	21.268500720807303	32.37113567195259	21.822721448021785
40	20.297573282075927	20.42419509851033	35.73526349511453	23.542968124299215
41	21.067755886592984	22.729216722729458	30.896283837898448	25.306743552779114
42	20.58361364728496	17.361164504244755	34.705750440493354	27.34947140797693
43	22.97949703668108	18.818676918148324	34.27578888354957	23.926037161621018
44	21.54885471728336	19.762493993272464	35.135992311388755	23.552658978055423
45	21.91866890917828	21.40701585776069	33.656014736504886	23.018300496556144
46	22.83049014896684	19.446500080089702	33.149127022264935	24.573882748678518
47	19.560707992952107	20.228455870575043	36.19069357680602	24.020142559666827
48	21.774827807144	18.437089540285122	32.699703668108285	27.088378984462597
49	21.264576325484544	16.56114848630466	36.3034999199103	25.870775268300495
50	19.93588819477815	16.932924875860962	38.30950664744514	24.821680281915746
51	18.351073201986225	15.902290565433287	36.72232900848951	29.024307224090983
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	723.0
1	3622.0
2	6521.0
3	80670.0
4	154819.0
5	87740.0
6	20661.0
7	17967.5
8	15274.0
9	14867.5
10	14461.0
11	14222.5
12	13984.0
13	13266.5
14	12549.0
15	11667.5
16	10786.0
17	10055.0
18	9324.0
19	8708.5
20	8093.0
21	7518.0
22	6943.0
23	7060.0
24	7177.0
25	8070.0
26	9485.0
27	10007.0
28	11111.0
29	12215.0
30	13440.5
31	14666.0
32	17328.0
33	19990.0
34	23769.5
35	27549.0
36	30598.0
37	33647.0
38	37623.5
39	41600.0
40	50295.0
41	58990.0
42	73752.5
43	88515.0
44	124255.5
45	159996.0
46	206714.5
47	253433.0
48	275936.0
49	298439.0
50	262175.5
51	225912.0
52	190867.5
53	155823.0
54	139293.0
55	122763.0
56	116750.0
57	110737.0
58	108161.5
59	105586.0
60	105700.0
61	105814.0
62	105512.5
63	105211.0
64	101070.5
65	96930.0
66	77090.0
67	57250.0
68	45174.5
69	33099.0
70	27728.5
71	22358.0
72	20259.0
73	18160.0
74	15742.0
75	10114.5
76	6905.0
77	5429.0
78	3953.0
79	3051.5
80	2150.0
81	1535.0
82	920.0
83	757.0
84	594.0
85	393.5
86	193.0
87	153.0
88	113.0
89	83.5
90	54.0
91	36.5
92	19.0
93	11.0
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2497200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.25622898621811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.05434115462356	19.639296219614994
2	11.38002220652278	6.203529822584639
3	4.360373135012408	3.5654198589975588
4	2.2113724295848507	2.410946932582967
5	1.283417420948752	1.7490559555140335
6	0.9350035757564108	1.5290802938249684
7	0.6970505628411278	1.3299278829038998
8	0.5217499917757992	1.137674979951888
9	0.44635029822552036	1.0949243342851376
>10	5.475033471791779	32.65923789862914
>50	0.4321178664928757	7.835706922466352
>100	0.18819074859941876	9.443403883235836
>500	0.0083041556255697	1.515629005816464
>1k	0.005634962745922297	3.1109620143922037
>5k	5.931539732549787E-4	1.1997230800728047
>10k+	4.44865479941234E-4	5.575480915127282
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115371	4.620014416146083	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12275	0.4915505366009931	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	10300	0.4124619573922793	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	9002	0.3604837417908057	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	8523	0.3413022585295531	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	6118	0.24499439372096748	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG	6040	0.2418708954028512	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	4223	0.16910940253083453	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4118	0.16490469325644722	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3936	0.15761653051417587	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCC	3574	0.1431202947300977	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT	3551	0.14219926317475573	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	3386	0.1355918628864328	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT	2916	0.11677078327727054	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2889	0.11568957232099952	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	2672	0.10699983982059907	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	2598	0.10403652090341182	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.004485023226013E-5	0.0	0.0	0.3692535639916707	0.0
2	4.004485023226013E-5	0.0	0.0	1.2079529072561268	0.0
3	4.004485023226013E-5	0.0	0.0	2.02250520583053	0.0
4	4.004485023226013E-5	0.0	0.0	2.5874979977574886	0.0
5	4.004485023226013E-5	0.0	0.0	5.321560147365049	0.0
6	4.004485023226013E-5	0.0	0.0	6.3551978215601475	0.0
7	4.004485023226013E-5	0.0	0.0	9.224771744353676	0.0
8	4.004485023226013E-5	0.0	0.0	12.69541886913343	0.0
9	4.004485023226013E-5	0.0	0.0	15.980017619734102	0.0
10	4.004485023226013E-5	0.0	0.0	17.797933685728015	0.0
11	4.004485023226013E-5	0.0	0.0	19.21207752683005	0.0
12	4.004485023226013E-5	0.0	0.0	20.22893640877783	0.0
13	4.004485023226013E-5	0.0	0.0	20.756166906935768	0.0
14	4.004485023226013E-5	0.0	0.0	21.09798974851834	0.0
15	4.004485023226013E-5	0.0	0.0	21.916626621816434	0.0
16	4.004485023226013E-5	0.0	0.0	22.810748037802338	0.0
17	4.004485023226013E-5	0.0	0.0	23.92379465000801	0.0
18	4.004485023226013E-5	0.0	0.0	24.564872657376263	0.0
19	4.004485023226013E-5	0.0	0.0	25.281555341983022	0.0
20	4.004485023226013E-5	0.0	0.0	26.046371936568956	0.0
21	4.004485023226013E-5	0.0	0.0	26.802178439852636	0.0
22	8.008970046452026E-5	0.0	0.0	27.549495434887074	0.0
23	8.008970046452026E-5	0.0	0.0	28.208473490309146	0.0
24	2.0022425116130065E-4	0.0	0.0	28.76769982380266	0.0
25	2.0022425116130065E-4	0.0	0.0	29.28756206951786	0.0
26	2.0022425116130065E-4	0.0	0.0	29.7935687970527	0.0
27	2.0022425116130065E-4	0.0	0.0	30.34867051097229	0.0
28	2.402691013935608E-4	0.0	0.0	30.867571680281916	0.0
29	2.402691013935608E-4	0.0	0.0	31.425276309466604	0.0
30	2.803139516258209E-4	0.0	0.0	31.932604517059108	0.0
31	2.803139516258209E-4	0.0	0.0	32.4476213358962	0.0
32	2.803139516258209E-4	0.0	0.0	33.04396924555502	0.0
33	2.803139516258209E-4	0.0	0.0	33.59562710235464	0.0
34	2.803139516258209E-4	0.0	0.0	34.1952586897325	0.0
35	2.803139516258209E-4	0.0	0.0	34.720366810828125	0.0
36	3.2035880185808104E-4	0.0	0.0	35.23782636552939	0.0
37	3.2035880185808104E-4	0.0	0.0	35.97208873938811	0.0
38	3.2035880185808104E-4	0.0	0.0	36.68440653531956	0.0
39	3.2035880185808104E-4	0.0	0.0	37.54613166746756	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTATCG	20	7.034877E-4	45.000004	10
CGTTTTT	27750	0.0	44.286484	1
ATAGCGG	340	0.0	42.35294	1
ACTACGG	170	0.0	42.35294	1
CGACGGT	410	0.0	42.256096	27
CACGACG	400	0.0	42.1875	25
ACGTAGG	140	0.0	41.785717	1
GCGATAG	205	0.0	41.707317	8
TACGCGG	310	0.0	41.370968	1
ATACCGG	60	3.6379788E-12	41.250004	1
TTTACGG	150	0.0	40.5	1
CGCACTT	3545	0.0	40.239777	34
AGGGCGA	1215	0.0	40.185184	5
TAACGGG	555	0.0	40.135136	2
GTTTTTT	31175	0.0	40.0417	2
CACTTAC	3565	0.0	40.014027	36
TCGATCC	45	1.9299478E-8	40.0	16
TAACCCG	90	0.0	40.0	37
GGGCGAT	2565	0.0	39.912277	6
TACGGGA	440	0.0	39.886364	3
>>END_MODULE