FastQCFastQC Report
Thu 26 May 2016
SRR1043244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043244_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences484705
Sequences flagged as poor quality0
Sequence length52
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9251919.08769251400336No Hit
CGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG16600.34247635159530027No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT14010.2890417883042263TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCTT12760.2632529064069898TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT10920.22529167225425775TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG8890.18341052805314573No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7070.14586191601076945No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7050.14544929390041367No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC6670.13760947380365376TruSeq Adapter, Index 16 (95% over 21bp)
CGGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT6140.12667498787922551No Hit
TCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT5350.11037641452017205TruSeq Adapter, Index 13 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC5270.10872592607874894No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCT206.3078234E-446.00000424
CAAACGG206.3078234E-446.0000041
CTGTAGG700.046.0000041
CTCAAGA206.3078234E-446.00000430
TGCTTAG206.3078234E-446.00000434
CATGCTT351.01792466E-746.00000411
TAGACGG750.046.0000041
TAGAAGG405.6006684E-946.0000041
ATTACGG405.6006684E-946.0000041
GCAGTAT206.3078234E-446.00000441
AGGGTCA800.046.0000045
GCACCTT206.3078234E-446.00000413
TCGGCAG206.3078234E-446.00000418
TATGAGG351.01792466E-746.0000041
CATTAAG206.3078234E-446.00000420
CTGCGCA206.3078234E-446.0000049
AACGGCC206.3078234E-446.0000045
GGTGGTA206.3078234E-446.0000048
GCTTAGG206.3078234E-446.0000041
ACTACGG206.3078234E-446.0000041
Produced by FastQC (version 0.11.5)