##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043244_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	484705
Sequences flagged as poor quality	0
Sequence length	52
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.721537842605294	34.0	31.0	34.0	31.0	34.0
2	32.99538688480622	34.0	33.0	34.0	31.0	34.0
3	32.87741616034495	34.0	31.0	34.0	31.0	34.0
4	36.42256836632591	37.0	37.0	37.0	35.0	37.0
5	36.35878111428601	37.0	37.0	37.0	35.0	37.0
6	36.51506380169381	37.0	37.0	37.0	35.0	37.0
7	36.4849527031906	37.0	37.0	37.0	35.0	37.0
8	36.50164533066504	37.0	37.0	37.0	35.0	37.0
9	38.176763185855314	39.0	39.0	39.0	37.0	39.0
10	38.02969435017175	39.0	39.0	39.0	35.0	39.0
11	37.62075076592979	39.0	37.0	39.0	35.0	39.0
12	37.53058045615374	39.0	37.0	39.0	35.0	39.0
13	37.53358021889603	39.0	37.0	39.0	35.0	39.0
14	39.07030668138352	41.0	38.0	41.0	35.0	41.0
15	39.124900712804696	41.0	38.0	41.0	35.0	41.0
16	39.11270566633313	41.0	38.0	41.0	35.0	41.0
17	39.12452110046317	41.0	38.0	41.0	35.0	41.0
18	38.29653500582829	39.0	38.0	40.0	35.0	41.0
19	37.388467212015556	37.0	37.0	40.0	35.0	41.0
20	36.482200513714524	35.0	35.0	40.0	34.0	41.0
21	36.42183802519058	35.0	35.0	40.0	34.0	41.0
22	36.371716817445666	35.0	35.0	40.0	34.0	41.0
23	36.35849434191931	35.0	35.0	40.0	34.0	41.0
24	36.2458691368977	35.0	35.0	40.0	34.0	41.0
25	36.176030781609434	35.0	35.0	40.0	34.0	41.0
26	36.08953693483665	35.0	35.0	40.0	34.0	41.0
27	36.04977666828277	35.0	35.0	40.0	34.0	41.0
28	35.903073003166874	35.0	35.0	40.0	33.0	41.0
29	35.741667612259	35.0	35.0	40.0	33.0	41.0
30	35.43474690791306	35.0	35.0	40.0	33.0	41.0
31	34.923768065111766	35.0	35.0	40.0	31.0	41.0
32	34.14605791151319	35.0	35.0	40.0	22.0	41.0
33	33.2070207652077	35.0	34.0	40.0	16.0	41.0
34	32.32885156951135	35.0	33.0	40.0	10.0	41.0
35	31.81412611794803	35.0	33.0	40.0	9.0	41.0
36	31.582077758636697	35.0	32.0	40.0	8.0	41.0
37	31.550831949330004	35.0	32.0	40.0	8.0	41.0
38	31.516842202989448	35.0	31.0	40.0	8.0	41.0
39	31.44026779174962	35.0	31.0	40.0	8.0	41.0
40	31.346125994161397	35.0	31.0	40.0	8.0	41.0
41	31.13871736416996	35.0	31.0	40.0	7.0	41.0
42	30.907785147667138	35.0	30.0	40.0	7.0	41.0
43	30.673126953507804	35.0	29.0	39.0	7.0	41.0
44	30.421437781743535	35.0	27.0	39.0	7.0	41.0
45	30.281490803684715	35.0	24.0	39.0	7.0	41.0
46	30.18728917589049	35.0	24.0	39.0	7.0	41.0
47	30.131312860399625	35.0	24.0	39.0	7.0	41.0
48	30.04746804757533	35.0	23.0	39.0	7.0	41.0
49	29.977807119794516	35.0	24.0	39.0	7.0	41.0
50	29.831264377301657	35.0	24.0	38.0	7.0	41.0
51	29.693838520337113	35.0	23.0	38.0	7.0	41.0
52	29.38245324475712	35.0	22.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	4.0
15	3.0
16	16.0
17	74.0
18	192.0
19	428.0
20	781.0
21	1342.0
22	2163.0
23	3750.0
24	7894.0
25	15903.0
26	23132.0
27	24040.0
28	18791.0
29	14316.0
30	11649.0
31	10519.0
32	11001.0
33	13478.0
34	26993.0
35	63246.0
36	78391.0
37	21820.0
38	35342.0
39	99073.0
40	363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.81599116988684	11.633880401481314	15.162418378188795	55.38771005044305
2	58.370967908315365	12.980266347572233	15.929482881340196	12.719282862772202
3	14.946617014472722	13.682961801508133	58.87519212717014	12.495229056849011
4	14.740718581405185	15.475598559948835	57.382325331902905	12.40135752674307
5	12.71453770850311	19.5772686479405	56.05987146821262	11.648322175343766
6	49.37250492567644	1.2923324496343136	47.58647012100143	1.7486925036878103
7	50.06385327157755	0.9591400955220186	46.50828854664177	2.468718086258652
8	45.54997369534046	1.9191054352647487	48.80597476815795	3.7249461012368346
9	27.035825914731642	12.784683467263594	51.89115028728815	8.288340330716622
10	21.96078026841068	11.43747227695196	58.17662289433779	8.425124560299563
11	20.320194757636088	11.125736272578166	58.04994790645856	10.504121063327178
12	12.149245417315687	19.294003569181253	58.77121135536048	9.785539658142582
13	8.634117659194768	21.263655212964586	59.75531508855902	10.346912039281625
14	7.866434222877833	13.77126293312427	67.60132451697423	10.760978327023654
15	8.42553718240992	11.067969177128356	67.76121558473712	12.745278055724615
16	9.047358702716085	12.44055662722687	58.765434645815496	19.74665002424155
17	10.930566014379881	12.91816671996369	61.887952465932884	14.263314799723542
18	13.990158962668014	14.72070640905293	58.50568902734653	12.783445600932525
19	14.774759905509537	13.667488472369794	57.63567530766136	13.92207631445931
20	11.049813804272702	17.437823005745763	58.95792286029648	12.554440329685065
21	11.810276353658411	13.391444280541773	57.90986269999278	16.88841666580704
22	9.671449644629208	16.28433789624617	57.51787169515479	16.526340763969838
23	9.510527021590452	13.284781465014802	64.67934104248975	12.525350470904984
24	10.924582993779723	13.440133689563755	62.25518614414953	13.38009717250699
25	9.067577186123518	18.04994790645857	58.17105249584799	14.711422411569924
26	9.469883743720407	18.093066916990747	58.42006993944771	14.016979399841139
27	10.933866991262727	15.224518005797341	61.525463941985336	12.316151060954601
28	8.980926542948804	14.448169505162934	60.24695433304794	16.323949618840324
29	10.732301090353927	17.67714382975212	57.739243457360665	13.851311622533293
30	16.274641276652808	15.343765795690162	54.62600963472628	13.755583292930751
31	14.909274713485521	15.002320999370752	54.59485666539442	15.493547621749313
32	14.490875893584757	16.094944347592865	53.85646939891274	15.557710359909635
33	11.809244798382522	16.944326961760243	52.90104290238392	18.345385337473306
34	12.014111676174167	15.58556235235865	52.21897855396581	20.181347417501367
35	17.051196088342394	16.774326652293663	49.22870612021745	16.94577113914649
36	13.444053599612134	20.192075592370617	49.562517407495285	16.80135340052197
37	13.851517933588472	20.243447045109914	48.903353586201916	17.001681435099698
38	13.10611609123075	20.36785261138218	48.75336544908759	17.772665848299482
39	15.980235400913958	17.69963173476651	49.37188599251091	16.948246871808625
40	12.184937229861461	17.066669417480735	50.56353864721841	20.184854705439392
41	13.925996224507688	18.72025252473154	46.75400501335864	20.59974623740213
42	14.45043892676989	17.20035898123601	48.265233492536694	20.083968599457403
43	14.711009789459567	19.251916113924963	47.05418759864247	18.982886497972995
44	12.339876832300058	22.877420286566057	44.83531220020425	19.94739068092964
45	14.770014751240446	20.559102959532087	45.84314170474825	18.82774058447922
46	15.336957530869292	18.396963101267783	47.01478218710349	19.25129718075943
47	13.41723316243901	17.319400460073652	49.99123178015494	19.2721345973324
48	14.236700673605595	16.00643690492155	48.72530714558339	21.031555275889456
49	14.299006612269318	18.898711587460415	47.924613940438	18.877667859832272
50	13.018021270669788	18.91851744875749	47.4505111356392	20.612950144933517
51	13.159138032411466	16.45495713887829	48.61967588533231	21.76622894337793
52	15.208425743493464	16.370988539420885	48.331871963359156	20.088713753726495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	164.0
1	526.0
2	888.0
3	50938.0
4	100988.0
5	55703.0
6	10418.0
7	10651.5
8	10885.0
9	11866.5
10	12848.0
11	13279.5
12	13711.0
13	13182.5
14	11761.5
15	10869.0
16	9886.5
17	8904.0
18	8171.5
19	7439.0
20	6847.5
21	6256.0
22	5700.0
23	5144.0
24	4909.0
25	4674.0
26	4482.5
27	4291.0
28	3949.0
29	3607.0
30	3493.0
31	3379.0
32	3614.5
33	3850.0
34	4466.0
35	5082.0
36	5505.5
37	5929.0
38	6268.5
39	7232.5
40	7857.0
41	11696.5
42	15536.0
43	20248.0
44	24960.0
45	27150.5
46	29341.0
47	31569.0
48	33797.0
49	33531.0
50	33265.0
51	30252.0
52	27239.0
53	22717.5
54	18196.0
55	15268.0
56	12340.0
57	11071.0
58	9802.0
59	8978.0
60	8154.0
61	7201.5
62	6249.0
63	5706.5
64	4414.0
65	3664.0
66	3138.0
67	2612.0
68	2247.5
69	1883.0
70	1799.0
71	1715.0
72	1702.5
73	1690.0
74	1412.5
75	1135.0
76	928.5
77	722.0
78	592.0
79	462.0
80	318.5
81	175.0
82	131.5
83	88.0
84	66.5
85	45.0
86	31.0
87	17.0
88	10.5
89	2.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	484705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.077522790276234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.36378998595278	34.21169238626376
2	6.739273657942873	5.401867872322275
3	2.118869166788228	2.5475708196470643
4	1.11767590770051	1.7917472665203948
5	0.6759599130018805	1.3545399409323002
6	0.524983413084922	1.2624020821456778
7	0.35891750753784174	1.0069167211723895
8	0.3052537736602164	0.9787052056548116
9	0.2596824559595476	0.9366686592258304
>10	2.273796065617599	20.098865187134365
>50	0.20086682726065583	5.225209052717872
>100	0.054734919510615584	3.952690914765383
>500	0.0036145701563614066	0.9610626496424314
>1k	0.0020654686607779467	1.1235161767676054
>5k	0.0	0.0
>10k+	5.163671651944867E-4	19.146545065087878
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92519	19.08769251400336	No Hit
CGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG	1660	0.34247635159530027	No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	1401	0.2890417883042263	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCTT	1276	0.2632529064069898	TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	1092	0.22529167225425775	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	889	0.18341052805314573	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	707	0.14586191601076945	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	705	0.14544929390041367	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	667	0.13760947380365376	TruSeq Adapter, Index 16 (95% over 21bp)
CGGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	614	0.12667498787922551	No Hit
TCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	535	0.11037641452017205	TruSeq Adapter, Index 13 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	527	0.10872592607874894	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27439370338659597	0.0
2	0.0	0.0	0.0	0.9744071136051825	0.0
3	0.0	0.0	0.0	1.56569459774502	0.0
4	0.0	0.0	0.0	2.2525041004322217	0.0
5	0.0	0.0	0.0	3.9256867579249235	0.0
6	0.0	0.0	0.0	4.255165513044016	0.0
7	0.0	0.0	0.0	5.310240249223755	0.0
8	0.0	0.0	0.0	6.624235359651747	0.0
9	0.0	0.0	0.0	7.515705429075417	0.0
10	0.0	0.0	0.0	8.28937188599251	0.0
11	0.0	0.0	0.0	9.139579744380603	4.126221103557834E-4
12	0.0	0.0	0.0	9.74077015916898	4.126221103557834E-4
13	0.0	0.0	0.0	10.04115905550799	4.126221103557834E-4
14	0.0	0.0	0.0	10.258817218720665	4.126221103557834E-4
15	0.0	0.0	0.0	10.87300522998525	4.126221103557834E-4
16	2.063110551778917E-4	0.0	0.0	11.534232161830392	4.126221103557834E-4
17	2.063110551778917E-4	0.0	0.0	12.31924572678227	4.126221103557834E-4
18	2.063110551778917E-4	0.0	0.0	12.743214945172838	4.126221103557834E-4
19	2.063110551778917E-4	0.0	0.0	13.181007004260323	4.126221103557834E-4
20	2.063110551778917E-4	0.0	0.0	13.6412869683622	4.126221103557834E-4
21	4.126221103557834E-4	0.0	0.0	14.102392176684788	4.126221103557834E-4
22	0.0012378663310673502	0.0	0.0	14.562672140786663	4.126221103557834E-4
23	0.0012378663310673502	0.0	0.0	14.939602438596673	4.126221103557834E-4
24	0.002063110551778917	0.0	0.0	15.231738892728567	4.126221103557834E-4
25	0.002063110551778917	0.0	0.0	15.531508855902043	4.126221103557834E-4
26	0.002888354772490484	0.0	0.0	15.840150194448169	4.126221103557834E-4
27	0.002888354772490484	0.0	0.0	16.132080337524886	4.126221103557834E-4
28	0.003300976882846267	0.0	0.0	16.4398964318503	4.126221103557834E-4
29	0.003300976882846267	0.0	0.0	16.79722717941841	4.126221103557834E-4
30	0.003300976882846267	0.0	0.0	17.119691358661456	4.126221103557834E-4
31	0.003300976882846267	0.0	0.0	17.403781681641412	4.126221103557834E-4
32	0.003300976882846267	0.0	0.0	17.707471554863268	4.126221103557834E-4
33	0.003300976882846267	0.0	0.0	17.991561877843225	4.126221103557834E-4
34	0.003300976882846267	0.0	0.0	18.337545517376547	4.126221103557834E-4
35	0.003300976882846267	0.0	0.0	18.631126148894687	4.126221103557834E-4
36	0.003300976882846267	0.0	0.0	18.8923159447499	4.126221103557834E-4
37	0.003919910048379943	0.0	0.0	19.172486357681475	4.126221103557834E-4
38	0.004332532158735726	0.0	0.0	19.461115523875346	4.126221103557834E-4
39	0.004745154269091509	0.0	0.0	19.748094201627794	4.126221103557834E-4
40	0.004745154269091509	0.0	0.0	20.0373423009872	4.126221103557834E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCT	20	6.3078234E-4	46.000004	24
CAAACGG	20	6.3078234E-4	46.000004	1
CTGTAGG	70	0.0	46.000004	1
CTCAAGA	20	6.3078234E-4	46.000004	30
TGCTTAG	20	6.3078234E-4	46.000004	34
CATGCTT	35	1.01792466E-7	46.000004	11
TAGACGG	75	0.0	46.000004	1
TAGAAGG	40	5.6006684E-9	46.000004	1
ATTACGG	40	5.6006684E-9	46.000004	1
GCAGTAT	20	6.3078234E-4	46.000004	41
AGGGTCA	80	0.0	46.000004	5
GCACCTT	20	6.3078234E-4	46.000004	13
TCGGCAG	20	6.3078234E-4	46.000004	18
TATGAGG	35	1.01792466E-7	46.000004	1
CATTAAG	20	6.3078234E-4	46.000004	20
CTGCGCA	20	6.3078234E-4	46.000004	9
AACGGCC	20	6.3078234E-4	46.000004	5
GGTGGTA	20	6.3078234E-4	46.000004	8
GCTTAGG	20	6.3078234E-4	46.000004	1
ACTACGG	20	6.3078234E-4	46.000004	1
>>END_MODULE