FastQCFastQC Report
Thu 26 May 2016
SRR1043243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043243_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences469054
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8756318.66799984649955No Hit
CGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT16910.36051286205852634No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC14040.2993258771911123TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT11870.25306254716940907TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC10900.2323826254546385TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC7570.1613886674029003No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7180.1530740597031472No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG6860.14625181748796515No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6580.14028235554968083No Hit
CGGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC5760.12280035987327685No Hit
TCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC5070.10808990009679056TruSeq Adapter, Index 13 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC5030.10723711981989281No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTATA302.1627438E-645.00000434
CATGCGG406.7993824E-945.0000041
CGCGGAT302.1627438E-645.00000422
ACGTTCA207.0290104E-445.00000416
CCTAGGC207.0290104E-445.00000424
TAGGTTA302.1627438E-645.0000045
GTGATTA302.1627438E-645.00000443
CGTCTGC207.0290104E-445.00000437
CAACAGG207.0290104E-445.0000041
ACTTAGG207.0290104E-445.0000041
ATCCGTC207.0290104E-445.00000434
AACGGCC302.1627438E-645.0000045
ACGGCCA302.1627438E-645.0000046
AACGCGG302.1627438E-645.0000041
GGAACGA207.0290104E-445.0000047
CACAGTA207.0290104E-445.00000425
CATACGA406.7993824E-945.00000417
TATAGGT302.1627438E-645.00000422
TGGATGG302.1627438E-645.0000041
ACGTAGG302.1627438E-645.0000041