##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043243_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	469054
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55020957075305	33.0	31.0	34.0	31.0	34.0
2	32.92610019315473	34.0	31.0	34.0	31.0	34.0
3	32.80148341129166	34.0	31.0	34.0	31.0	34.0
4	36.401783163558996	37.0	37.0	37.0	35.0	37.0
5	36.39045184562971	37.0	37.0	37.0	35.0	37.0
6	36.52635943835891	37.0	37.0	37.0	35.0	37.0
7	36.52187168215173	37.0	37.0	37.0	35.0	37.0
8	36.474292938552914	37.0	37.0	37.0	35.0	37.0
9	38.156235742579746	39.0	39.0	39.0	37.0	39.0
10	37.99614969704981	39.0	39.0	39.0	35.0	39.0
11	37.628281605103034	39.0	37.0	39.0	35.0	39.0
12	37.527902970660094	39.0	37.0	39.0	35.0	39.0
13	37.53290239503341	39.0	37.0	39.0	35.0	39.0
14	39.085559445181154	41.0	38.0	41.0	35.0	41.0
15	39.14717921603909	41.0	38.0	41.0	35.0	41.0
16	39.15298451777407	41.0	38.0	41.0	35.0	41.0
17	39.14877391515689	41.0	38.0	41.0	35.0	41.0
18	38.34458079453538	39.0	38.0	40.0	35.0	41.0
19	37.469532292657135	37.0	37.0	40.0	35.0	41.0
20	36.585921876798835	35.0	35.0	40.0	35.0	41.0
21	36.52357724270553	35.0	35.0	40.0	35.0	41.0
22	36.48766026939329	35.0	35.0	40.0	35.0	41.0
23	36.44575251463584	35.0	35.0	40.0	35.0	41.0
24	36.34353613869619	35.0	35.0	40.0	35.0	41.0
25	36.3128829516431	35.0	35.0	40.0	34.0	41.0
26	36.19982560643337	35.0	35.0	40.0	34.0	41.0
27	36.1822817841869	35.0	35.0	40.0	34.0	41.0
28	36.05965837622107	35.0	35.0	40.0	34.0	41.0
29	35.90129921075185	35.0	35.0	40.0	33.0	41.0
30	35.55931939606101	35.0	35.0	40.0	33.0	41.0
31	35.02602259014954	35.0	35.0	40.0	31.0	41.0
32	34.24031774593117	35.0	35.0	40.0	23.0	41.0
33	33.26968323476615	35.0	35.0	40.0	17.0	41.0
34	32.371377282786206	35.0	33.0	40.0	10.0	41.0
35	31.88075146998	35.0	33.0	40.0	10.0	41.0
36	31.641712894464177	35.0	33.0	40.0	8.0	41.0
37	31.586237405501286	35.0	32.0	40.0	8.0	41.0
38	31.53559078485633	35.0	31.0	40.0	8.0	41.0
39	31.429206445313334	35.0	31.0	40.0	8.0	41.0
40	31.30472824024526	35.0	31.0	40.0	8.0	41.0
41	31.065864484686198	35.0	31.0	40.0	7.0	41.0
42	30.797694934911544	35.0	30.0	39.0	7.0	41.0
43	30.543120834701334	35.0	27.0	39.0	7.0	41.0
44	30.373722428547673	35.0	26.0	39.0	7.0	41.0
45	30.261426189735083	35.0	24.0	39.0	7.0	41.0
46	30.13333646019435	35.0	24.0	39.0	7.0	41.0
47	30.026001270642613	35.0	23.0	39.0	7.0	41.0
48	29.959699736064504	35.0	23.0	38.0	7.0	41.0
49	29.86202654705002	35.0	24.0	38.0	7.0	41.0
50	29.72372903759482	35.0	24.0	38.0	7.0	41.0
51	29.356263884328882	35.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	4.0
16	16.0
17	50.0
18	142.0
19	282.0
20	570.0
21	980.0
22	1656.0
23	2952.0
24	5917.0
25	12554.0
26	20824.0
27	24089.0
28	20749.0
29	15091.0
30	12083.0
31	10589.0
32	10788.0
33	12969.0
34	26962.0
35	63067.0
36	72562.0
37	21606.0
38	36683.0
39	95823.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.237772196804634	11.737241341082264	15.290137169707538	54.73484929240556
2	57.956653178525286	13.122369705833444	16.006259407232427	12.91471770840884
3	15.205711922294663	13.96704857010067	58.17112741816507	12.656112089439594
4	14.95435493567905	15.57837690329898	56.786425443552346	12.68084271746963
5	12.991894323468086	19.75294955378272	55.36441433182533	11.890741790923858
6	50.07163354325941	1.274906513962145	46.87733182106964	1.7761281217088012
7	50.82165379679099	0.9018151428193769	45.82500095937781	2.451530101011824
8	46.20576735301266	1.897009725959058	48.04286926451965	3.854353656508632
9	27.406439343870854	13.029416655651588	51.18110068350339	8.383043316974165
10	22.4684151504944	11.554319971687695	57.45905588695545	8.51820899086246
11	20.583557543481135	11.244973926243034	57.60232297347427	10.569145556801562
12	12.430338511130914	19.503298127720903	58.22762411150955	9.838739249638634
13	8.839707155252913	21.62458906650407	59.17932690052744	10.356376877715572
14	8.04086523086894	13.910125486617744	67.1008028926307	10.948206389882614
15	8.538036132300332	11.16843689639146	67.49585335590359	12.797673615404623
16	9.228361766449066	12.381303645209293	58.279004123192635	20.111330465149003
17	11.080174137732543	13.075893180742515	61.2699177493423	14.57401493218265
18	14.253582743138315	14.896365876850002	58.01080472610829	12.839246653903388
19	15.044536449960985	13.884542078310814	57.01582333803784	14.055098133690363
20	11.232821807297242	17.606927986969517	58.34317583902919	12.817074366704048
21	11.959390603214128	13.573703667381581	57.40639670485701	17.06050902454728
22	9.802922478008929	16.301960968246725	56.97425029953907	16.920866254205272
23	9.627249740967992	13.51123751209882	64.10413299961199	12.757379747321204
24	11.147970169745914	13.61442392560345	61.7594136282816	13.478192276369032
25	9.149905980974472	18.27550772405736	57.70401702149432	14.870569273473844
26	9.660934561905453	18.13053507698474	57.94066354833345	14.267866812776353
27	11.236232928404831	15.240889108716694	61.13709722121546	12.385780741663007
28	9.109612112891053	14.57742605329024	59.74109590793384	16.571865925884865
29	10.882968698699937	17.753606194595932	57.25843932681525	14.104985779888882
30	16.630068179783137	15.371577686151275	54.09462449952458	13.903729634541012
31	15.170108345734178	15.09122617012114	53.99122489095072	15.747440593193959
32	14.664622836603037	16.297910261931463	53.340127149539285	15.697339751926217
33	12.025054684535256	17.050702051362958	52.27607055904011	18.64817270506168
34	12.141032802193351	15.653847957804432	51.89956806678975	20.305551173212464
35	17.329987592046972	16.65714395357464	48.994572053537546	17.01829640084084
36	13.71249365744669	20.23178567926081	49.196680979162316	16.859039684130188
37	14.070021788536074	20.196608492838777	48.59291254311871	17.140457175506445
38	13.35176760031894	20.378250691818	48.556882576419774	17.71309913144329
39	16.321148524476925	17.53017776204872	49.13165648304886	17.017017230425495
40	12.37725293889403	16.957322611042652	50.454531887586505	20.210892562476815
41	14.200923561039879	18.64199004805417	46.511702277349734	20.645384113556222
42	14.741159866454609	17.060722219616505	47.963773893837384	20.234344020091502
43	14.905746459895875	19.04215719298844	46.89673257236907	19.15536377474662
44	12.57275281737284	22.747913886247638	44.70721921143408	19.972114084945446
45	14.949451449086887	20.431762654193335	45.66553104759793	18.95325484912185
46	15.632528450881988	18.426236637999036	46.76178009355	19.17945481756898
47	13.722940215838689	17.126599496006854	49.94435608693242	19.206104201222036
48	14.365296959411921	15.936757814665262	48.67328708421631	21.0246581417065
49	14.437996478017457	18.770973064934953	47.850780507148436	18.94024994989916
50	13.17311013230886	18.825337807587186	47.458075189637015	20.54347687046694
51	13.447918576539161	16.675905119666393	47.80878107851122	22.067395225283228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	431.5
2	787.0
3	48238.5
4	95690.0
5	53449.0
6	11208.0
7	11385.5
8	11563.0
9	12674.5
10	13786.0
11	13970.5
12	14155.0
13	13333.5
14	12512.0
15	11198.5
16	9885.0
17	8904.0
18	7923.0
19	7175.5
20	6428.0
21	5861.5
22	5295.0
23	4808.0
24	4321.0
25	4111.5
26	3744.5
27	3587.0
28	3412.5
29	3238.0
30	3297.0
31	3356.0
32	3506.0
33	3656.0
34	4463.0
35	5270.0
36	5633.0
37	5996.0
38	6786.5
39	7577.0
40	9758.0
41	11939.0
42	16073.0
43	20207.0
44	22134.0
45	24061.0
46	26155.0
47	28249.0
48	31012.5
49	33776.0
50	34279.0
51	34782.0
52	28507.5
53	22233.0
54	18944.0
55	15655.0
56	13097.5
57	10540.0
58	10104.5
59	9669.0
60	8182.0
61	6695.0
62	5994.5
63	5294.0
64	4709.0
65	4124.0
66	3464.0
67	2804.0
68	2491.5
69	2179.0
70	1970.5
71	1762.0
72	1791.0
73	1820.0
74	1504.0
75	1041.0
76	894.0
77	713.5
78	533.0
79	382.0
80	231.0
81	169.0
82	107.0
83	85.5
84	64.0
85	40.5
86	17.0
87	14.0
88	11.0
89	7.0
90	3.0
91	3.5
92	4.0
93	2.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	469054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.711729844585705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.95765388157996	33.73815399175121
2	6.865238178067737	5.452609676923234
3	2.205321296196814	2.627313706052383
4	1.1350146581952387	1.8029358190357643
5	0.6964514497207754	1.3828645910590747
6	0.5111945853856609	1.218025276371022
7	0.3713040066892943	1.0321587082700248
8	0.32018039381245683	1.0171933840490677
9	0.25498625484414306	0.9113350739807964
>10	2.421299470631467	20.820267751131507
>50	0.20141678239446603	5.228902899855814
>100	0.053459145997458796	3.894657745537289
>500	0.0037799396159819356	0.944607827832384
>1k	0.0021599654948468204	1.1519712261329322
>5k	0.0	0.0
>10k+	5.399913737117051E-4	18.777002322017488
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87563	18.66799984649955	No Hit
CGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	1691	0.36051286205852634	No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	1404	0.2993258771911123	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	1187	0.25306254716940907	TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	1090	0.2323826254546385	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC	757	0.1613886674029003	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	718	0.1530740597031472	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG	686	0.14625181748796515	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	658	0.14028235554968083	No Hit
CGGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	576	0.12280035987327685	No Hit
TCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	507	0.10808990009679056	TruSeq Adapter, Index 13 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	503	0.10723711981989281	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26159034993838665	0.0
2	0.0	0.0	0.0	0.9862403902322547	0.0
3	0.0	0.0	0.0	1.6096227726445143	0.0
4	0.0	0.0	0.0	2.3152984517774073	0.0
5	0.0	0.0	0.0	4.024056931611286	0.0
6	0.0	0.0	0.0	4.365595432508837	0.0
7	0.0	0.0	0.0	5.417499904062219	0.0
8	0.0	0.0	0.0	6.789196979452259	0.0
9	0.0	0.0	0.0	7.703803826425102	0.0
10	0.0	0.0	0.0	8.523325672523846	0.0
11	0.0	0.0	0.0	9.386978897952048	0.0
12	0.0	0.0	0.0	10.035731493602016	0.0
13	0.0	0.0	0.0	10.348062270015818	0.0
14	0.0	0.0	0.0	10.564455265278625	0.0
15	0.0	0.0	0.0	11.160548678830157	0.0
16	0.0	0.0	0.0	11.853219458740359	0.0
17	0.0	0.0	0.0	12.692994836415423	0.0
18	0.0	0.0	0.0	13.126420412148708	0.0
19	0.0	0.0	0.0	13.569439765997092	0.0
20	2.13195069224439E-4	0.0	0.0	14.04720991612906	0.0
21	4.26390138448878E-4	0.0	0.0	14.51069599662299	0.0
22	0.0012791704153466339	0.0	0.0	15.012983579715769	0.0
23	0.0014923654845710728	0.0	0.0	15.40696806764253	0.0
24	0.00213195069224439	0.0	0.0	15.714608552533397	0.0
25	0.00213195069224439	0.0	0.0	16.010523308616918	0.0
26	0.0029847309691421455	0.0	0.0	16.3121943315695	0.0
27	0.0031979260383665845	0.0	0.0	16.6157841101451	0.0
28	0.003411121107591024	0.0	0.0	16.91980027885915	0.0
29	0.003411121107591024	0.0	0.0	17.30269862318624	0.0
30	0.003411121107591024	0.0	0.0	17.644663514222245	0.0
31	0.003411121107591024	0.0	0.0	17.945481756897927	0.0
32	0.003411121107591024	0.0	0.0	18.235000660904713	0.0
33	0.003411121107591024	0.0	0.0	18.525585540257627	0.0
34	0.003411121107591024	0.0	0.0	18.88354006148546	0.0
35	0.003411121107591024	0.0	0.0	19.176896476738285	0.0
36	0.003411121107591024	0.0	0.0	19.454476456868505	0.0
37	0.004050706315264341	0.0	0.0	19.751883578436598	0.0
38	0.00426390138448878	0.0	0.0	20.046519164104772	0.0
39	0.004903486592162097	0.0	0.0	20.344352675811315	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTATA	30	2.1627438E-6	45.000004	34
CATGCGG	40	6.7993824E-9	45.000004	1
CGCGGAT	30	2.1627438E-6	45.000004	22
ACGTTCA	20	7.0290104E-4	45.000004	16
CCTAGGC	20	7.0290104E-4	45.000004	24
TAGGTTA	30	2.1627438E-6	45.000004	5
GTGATTA	30	2.1627438E-6	45.000004	43
CGTCTGC	20	7.0290104E-4	45.000004	37
CAACAGG	20	7.0290104E-4	45.000004	1
ACTTAGG	20	7.0290104E-4	45.000004	1
ATCCGTC	20	7.0290104E-4	45.000004	34
AACGGCC	30	2.1627438E-6	45.000004	5
ACGGCCA	30	2.1627438E-6	45.000004	6
AACGCGG	30	2.1627438E-6	45.000004	1
GGAACGA	20	7.0290104E-4	45.000004	7
CACAGTA	20	7.0290104E-4	45.000004	25
CATACGA	40	6.7993824E-9	45.000004	17
TATAGGT	30	2.1627438E-6	45.000004	22
TGGATGG	30	2.1627438E-6	45.000004	1
ACGTAGG	30	2.1627438E-6	45.000004	1
>>END_MODULE