FastQCFastQC Report
Thu 26 May 2016
SRR1043242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043242_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences762232
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516606.777464079177993No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT50550.6631839125095771Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT44730.5868292068556555Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT35790.46954208167592015Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC22530.29557929869121213Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT18840.2471688409827979Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG16970.22263562799777495No Hit
ACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC14170.18590140534640373Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC12720.16687832575908648Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT11370.14916718269503249No Hit
CGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG10380.13617901111472622No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC10000.13119365232632582Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC9770.12817619832282034Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT9440.1238468077960516Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT7860.1031182107284921No Hit
TGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC7840.10285582342383946Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG7770.10193746785755518No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT7690.10088791863894458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGG700.046.0000041
TCGGGTA351.0189615E-746.0000044
ACGGGTC551.8189894E-1246.0000044
TCGACGT551.8189894E-1246.00000425
TACACGG700.046.0000041
CGGACAA206.310109E-446.014
CTGTCGG301.8605715E-646.01
ATCTCGG301.8605715E-646.01
GGTACGA206.310109E-446.07
ACCGGGT253.4160657E-546.03
GTCGATT206.310109E-446.019
CTCCGTT253.4160657E-546.021
CTACTCG253.4160657E-546.042
GGTAACG206.310109E-446.027
CAACGGT206.310109E-446.030
CAACGGC253.4160657E-546.02
CCGATCT253.4160657E-546.011
CTATCGG253.4160657E-546.01
CGGCCAC453.092282E-1046.022
CCGGCCA453.092282E-1046.021
Produced by FastQC (version 0.11.5)