##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043242_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	762232
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26087726571438	33.0	31.0	34.0	31.0	34.0
2	32.59030715057882	34.0	31.0	34.0	31.0	34.0
3	32.664329233094385	34.0	31.0	34.0	31.0	34.0
4	36.17133366219209	37.0	35.0	37.0	35.0	37.0
5	36.07219061912908	37.0	35.0	37.0	35.0	37.0
6	36.31312776162638	37.0	37.0	37.0	35.0	37.0
7	36.24918135160949	37.0	37.0	37.0	35.0	37.0
8	36.22505877475624	37.0	37.0	37.0	35.0	37.0
9	37.75812482288857	39.0	38.0	39.0	35.0	39.0
10	37.58797846325003	39.0	37.0	39.0	35.0	39.0
11	37.23791181687465	39.0	37.0	39.0	35.0	39.0
12	37.21190923498357	39.0	37.0	39.0	34.0	39.0
13	37.226368087406456	39.0	37.0	39.0	34.0	39.0
14	38.63336228339928	40.0	38.0	41.0	35.0	41.0
15	38.74130842053338	40.0	38.0	41.0	35.0	41.0
16	38.753664238709476	40.0	38.0	41.0	35.0	41.0
17	38.73847463764313	40.0	38.0	41.0	35.0	41.0
18	38.466918208629394	40.0	38.0	41.0	35.0	41.0
19	38.179413879238865	40.0	37.0	41.0	35.0	41.0
20	37.874416188247146	40.0	35.0	41.0	34.0	41.0
21	37.79658686594108	40.0	35.0	41.0	34.0	41.0
22	37.74748239381186	39.0	35.0	41.0	34.0	41.0
23	37.730034949988976	39.0	35.0	41.0	34.0	41.0
24	37.634806725511396	39.0	35.0	41.0	34.0	41.0
25	37.52487169260802	39.0	35.0	41.0	34.0	41.0
26	37.49375911795884	39.0	35.0	41.0	34.0	41.0
27	37.51398917914756	39.0	35.0	41.0	34.0	41.0
28	37.4554308924317	39.0	35.0	41.0	34.0	41.0
29	37.431838337933854	39.0	35.0	41.0	33.0	41.0
30	37.37452901478815	39.0	35.0	41.0	33.0	41.0
31	37.23001527094113	39.0	35.0	41.0	33.0	41.0
32	37.05304815331815	39.0	35.0	41.0	33.0	41.0
33	36.85009419704237	39.0	35.0	41.0	33.0	41.0
34	36.63946410016898	39.0	35.0	41.0	32.0	41.0
35	36.52554471604446	39.0	35.0	41.0	31.0	41.0
36	36.36791160696481	39.0	35.0	41.0	31.0	41.0
37	36.401671669517945	39.0	35.0	41.0	31.0	41.0
38	36.29291081980289	39.0	35.0	41.0	31.0	41.0
39	36.192915017999766	39.0	35.0	41.0	31.0	41.0
40	36.16749231205198	39.0	35.0	41.0	31.0	41.0
41	36.190512862225674	39.0	35.0	41.0	31.0	41.0
42	36.13109263321403	39.0	35.0	41.0	31.0	41.0
43	36.03816685733477	39.0	35.0	41.0	31.0	41.0
44	35.93533071295879	38.0	35.0	41.0	30.0	41.0
45	35.88531182107285	38.0	35.0	41.0	30.0	41.0
46	35.846599198144396	38.0	35.0	41.0	30.0	41.0
47	35.763242687265816	38.0	35.0	40.0	30.0	41.0
48	35.700999170856115	38.0	35.0	40.0	30.0	41.0
49	35.65993949348755	38.0	35.0	40.0	29.0	41.0
50	35.525191805119704	38.0	35.0	40.0	28.0	41.0
51	35.455664417132844	38.0	35.0	40.0	28.0	41.0
52	35.15934649817903	37.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	0.0
14	1.0
15	4.0
16	11.0
17	50.0
18	150.0
19	240.0
20	444.0
21	777.0
22	1280.0
23	2218.0
24	4074.0
25	7271.0
26	10348.0
27	10902.0
28	10170.0
29	9879.0
30	11256.0
31	13309.0
32	16705.0
33	22469.0
34	41340.0
35	82688.0
36	79452.0
37	57647.0
38	97120.0
39	282130.0
40	294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.75576464908322	19.30317803503395	25.19993912614532	28.74111818973751
2	32.841444599544495	20.09781798717451	26.457955058302456	20.602782354978537
3	21.66072796733803	21.43494369168442	37.83323187690887	19.07109646406868
4	22.52726204095341	24.40661111052803	34.93017873823193	18.13594811028663
5	19.231546300863776	30.153680244335057	31.259905120750638	19.354868334050526
6	73.07079734254138	1.7234910106109427	21.171900418770136	4.033811228077541
7	73.82266816402355	1.3892095844834644	17.840500005247744	6.9476222462452375
8	62.358573242792225	3.1776414529959385	25.461276881579366	9.002508422632479
9	33.873151481438725	19.456674608255753	29.397742419630767	17.272431490674755
10	26.414267572077794	19.01612632374395	41.92988486445072	12.639721239727535
11	23.901909130028653	16.358405314917242	39.832623138362074	19.907062416692032
12	17.08443098689113	22.84829290819593	41.934607835934465	18.132668268978474
13	12.394520303529633	26.164606051700794	40.91497077005426	20.52590287471531
14	10.814686342216017	22.544317215755832	45.77057903630391	20.870417405724243
15	11.190293768826288	18.183964987038067	48.818338773496784	21.807402470638863
16	11.808871839544915	24.270300905760976	39.263767461875126	24.657059792818984
17	14.45754048636111	22.00104430147252	41.5196423136263	22.021772898540075
18	16.487762256111	28.065733267561583	38.20083649072723	17.245667985600186
19	17.108308231614522	23.60606744403279	35.90284847657931	23.38277584777338
20	13.402874715309773	30.164175736521166	34.37509839523925	22.057851152929818
21	14.40637496195384	24.225039095708393	35.62458673999517	25.743999202342593
22	11.56485164621795	27.38890521521007	32.59821156813148	28.448031570440495
23	11.69945633350476	23.233083890469043	44.26145845359418	20.806001322432017
24	16.430168242739743	22.216857859549325	39.501359166238096	21.851614731472832
25	12.764224015785219	26.46792577587926	37.1930068535564	23.574843354779123
26	13.861265336537956	23.933526800239296	36.4166028190892	25.788605044133543
27	18.431133828020865	24.938076596101975	37.31121233430242	19.31957724157474
28	13.63797374027855	25.23418066940249	34.11979554781222	27.008050042506742
29	15.438606618457374	26.30065386916319	31.725642586509096	26.535096925870338
30	19.20150295448105	29.028563481984488	28.364067632951645	23.405865930582813
31	17.736594632605296	28.064290137385996	28.171344157684274	26.027771072324434
32	15.15444116751855	31.181976091268798	28.485159374048845	25.178423367163806
33	12.715551170772155	29.247525687717125	30.622828745053997	27.414094396456722
34	12.766060726917788	34.04226009928736	31.188666967537443	22.003012206257413
35	19.860882251073164	29.25356059572414	28.8686384197987	22.016918733404005
36	14.286332770075253	30.655102383526277	32.93852790226598	22.120036944132497
37	17.05373167224677	29.54664721502115	33.467500708445726	19.932120404286362
38	17.407692146223198	27.712166374542136	32.756037531880054	22.124103947354612
39	21.906191290840585	22.144963738074498	36.012001595314814	19.93684337577011
40	15.027970486675974	20.479197934487136	40.16887246927445	24.323959109562445
41	18.28996945811774	25.09039242645284	31.3080531911544	25.31158492427502
42	18.61349300475446	21.979790929795655	36.59883080217047	22.80788526327942
43	20.34236295511078	24.222939997271173	34.23275328246518	21.201943765152865
44	14.014105941498126	28.37666222357497	33.313610554266944	24.295621280659958
45	18.311222829794605	24.750469673275326	32.91976720998331	24.018540286946756
46	21.46105122849736	23.00533171003054	32.710775721827474	22.822841339644622
47	16.22458778954439	20.747357759842146	37.64864240808573	25.379412042527733
48	16.86205774619801	18.80477334984624	34.117171674765686	30.215997229190062
49	19.06191290840584	19.505478646921148	37.67422517028936	23.75838327438365
50	17.397196654037092	18.849510385289516	37.69692167214181	26.056371288531576
51	15.364351011240673	17.504775448944677	37.77130847301084	29.359565066803807
52	15.304526705779868	17.351016488418225	44.326530505148035	23.01792630065387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	1275.0
2	2335.0
3	29106.5
4	55878.0
5	30336.5
6	4795.0
7	4567.5
8	4340.0
9	4688.5
10	5037.0
11	5180.5
12	5324.0
13	5307.5
14	4965.0
15	4639.0
16	4208.5
17	3778.0
18	3471.0
19	3164.0
20	2947.5
21	2731.0
22	2600.0
23	2469.0
24	2462.5
25	2456.0
26	3200.5
27	3945.0
28	3914.0
29	3883.0
30	4572.5
31	5262.0
32	6336.0
33	7410.0
34	9408.5
35	11407.0
36	12243.0
37	13079.0
38	14566.5
39	19079.5
40	22105.0
41	31273.0
42	40441.0
43	57140.5
44	73840.0
45	92145.0
46	110450.0
47	112327.5
48	114205.0
49	99038.5
50	83872.0
51	66903.5
52	49935.0
53	40053.0
54	30171.0
55	24930.0
56	19689.0
57	17755.0
58	15821.0
59	13773.5
60	11726.0
61	9870.0
62	8014.0
63	6484.0
64	4226.5
65	3499.0
66	3114.0
67	2729.0
68	2231.5
69	1734.0
70	1614.0
71	1494.0
72	1686.5
73	1879.0
74	1498.5
75	1118.0
76	826.0
77	534.0
78	425.0
79	316.0
80	216.5
81	117.0
82	76.5
83	36.0
84	32.0
85	28.0
86	21.0
87	14.0
88	14.0
89	9.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	762232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.185805892844837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34144890981645	23.18397995494377
2	11.421930522131804	7.124042163695246
3	4.365996464544336	4.084713548163794
4	2.1814107730418333	2.721162117625729
5	1.2464511293187577	1.943579148692601
6	0.8206453995449862	1.535549288223962
7	0.5818040629011899	1.2700820001313498
8	0.45936866139722615	1.1460625526071888
9	0.3537884175884934	0.9929859226246338
>10	3.5590875630380063	24.96495690354576
>50	0.4865966617463331	10.10664735563383
>100	0.16712253077328407	8.936613169480653
>500	0.009706611635822053	2.057496556434972
>1k	0.003798239335756456	2.4677323887389324
>5k	4.220265928618284E-4	0.6653006520040161
>10k+	4.220265928618284E-4	6.799096277453506
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51660	6.777464079177993	No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	5055	0.6631839125095771	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT	4473	0.5868292068556555	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	3579	0.46954208167592015	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	2253	0.29557929869121213	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	1884	0.2471688409827979	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG	1697	0.22263562799777495	No Hit
ACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1417	0.18590140534640373	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1272	0.16687832575908648	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	1137	0.14916718269503249	No Hit
CGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	1038	0.13617901111472622	No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1000	0.13119365232632582	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	977	0.12817619832282034	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	944	0.1238468077960516	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT	786	0.1031182107284921	No Hit
TGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	784	0.10285582342383946	Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	777	0.10193746785755518	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT	769	0.10088791863894458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6097880960127625	0.0
2	0.0	0.0	0.0	2.17492836826583	0.0
3	0.0	0.0	0.0	3.6323586519589837	0.0
4	0.0	0.0	0.0	4.633235025556523	0.0
5	0.0	0.0	0.0	9.371424972974108	0.0
6	0.0	0.0	0.0	10.731247179336474	0.0
7	0.0	0.0	0.0	14.856631576737792	0.0
8	0.0	0.0	0.0	19.80210749483097	0.0
9	0.0	0.0	0.0	23.47959676319021	0.0
10	0.0	0.0	0.0	25.6184468770663	0.0
11	0.0	0.0	0.0	27.571395585595987	0.0
12	0.0	0.0	0.0	28.94630506197588	0.0
13	0.0	0.0	0.0	29.53418381805015	0.0
14	0.0	0.0	0.0	29.933537295731483	0.0
15	0.0	0.0	0.0	31.24403068881915	0.0
16	0.0	0.0	0.0	32.79696995140587	0.0
17	0.0	0.0	0.0	34.49330387598526	0.0
18	0.0	0.0	0.0	35.346980971672664	0.0
19	0.0	0.0	0.0	36.24736300758824	0.0
20	0.0	0.0	0.0	37.24653386370554	0.0
21	3.9358095697897754E-4	0.0	0.0	38.225763284669235	0.0
22	7.871619139579551E-4	0.0	0.0	39.219948781998134	0.0
23	7.871619139579551E-4	0.0	0.0	39.98454538775596	0.0
24	0.002492679394200191	0.0	0.0	40.58751141384775	0.0
25	0.002492679394200191	0.0	0.0	41.146265179105576	0.0
26	0.002492679394200191	0.0	0.0	41.721418150904185	0.0
27	0.002492679394200191	0.0	0.0	42.301687675143526	0.0
28	0.0026238730465265167	0.0	0.0	42.88182600573054	0.0
29	0.002755066698852843	0.0	0.0	43.59906170299856	0.0
30	0.002755066698852843	0.0	0.0	44.20163414813338	0.0
31	0.002755066698852843	0.0	0.0	44.70699209689438	0.0
32	0.002755066698852843	0.0	0.0	45.24882188100211	0.0
33	0.002755066698852843	0.0	0.0	45.765593677515504	0.0
34	0.002755066698852843	0.0	0.0	46.39283052928767	0.0
35	0.002755066698852843	0.0	0.0	46.87641033176251	0.0
36	0.002755066698852843	0.0	0.0	47.33729363238489	0.0
37	0.002755066698852843	0.0	0.0	47.81693762528994	0.0
38	0.002755066698852843	0.0	0.0	48.28700448157517	0.0
39	0.002755066698852843	0.0	0.0	48.75287314098595	0.0
40	0.002755066698852843	0.0	0.0	49.20680317803503	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGG	70	0.0	46.000004	1
TCGGGTA	35	1.0189615E-7	46.000004	4
ACGGGTC	55	1.8189894E-12	46.000004	4
TCGACGT	55	1.8189894E-12	46.000004	25
TACACGG	70	0.0	46.000004	1
CGGACAA	20	6.310109E-4	46.0	14
CTGTCGG	30	1.8605715E-6	46.0	1
ATCTCGG	30	1.8605715E-6	46.0	1
GGTACGA	20	6.310109E-4	46.0	7
ACCGGGT	25	3.4160657E-5	46.0	3
GTCGATT	20	6.310109E-4	46.0	19
CTCCGTT	25	3.4160657E-5	46.0	21
CTACTCG	25	3.4160657E-5	46.0	42
GGTAACG	20	6.310109E-4	46.0	27
CAACGGT	20	6.310109E-4	46.0	30
CAACGGC	25	3.4160657E-5	46.0	2
CCGATCT	25	3.4160657E-5	46.0	11
CTATCGG	25	3.4160657E-5	46.0	1
CGGCCAC	45	3.092282E-10	46.0	22
CCGGCCA	45	3.092282E-10	46.0	21
>>END_MODULE