FastQCFastQC Report
Thu 26 May 2016
SRR1043241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043241_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences747653
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480196.42263188939254No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC49600.6634093623646263Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT43780.5855657637968417Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC36220.48444933679126545Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG22520.3012092508155521No Hit
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC17580.23513581835423653Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCC15150.20263410967387277No Hit
ACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG14390.19246896621828577No Hit
GCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG12960.17334244629527334No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC10960.14659206878057066No Hit
CGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT10610.1419107527154977No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG10250.13709568476285122No Hit
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC9360.12519176676880853Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG9030.12077795447888258No Hit
TGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT8480.11342160066233935Illumina Single End Adapter 1 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT8190.10954279592270746No Hit
TGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG7710.10312270531917882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC1200.045.00000424
TACCCGG302.1644792E-645.0000041
ACCGGCG302.1644792E-645.00000419
GTCGATT302.1644792E-645.00000419
CCCTCCG600.045.00000422
GGTCGAT302.1644792E-645.00000418
GACGTGT600.045.00000443
CGTGACA302.1644792E-645.00000426
ATTGTCG351.2111741E-745.0000044
TCGATGG750.045.0000041
ACGGGTC600.045.0000044
ATCCGTG302.1644792E-645.00000423
ACGGCCA351.2111741E-745.0000046
TATTCGG302.1644792E-645.0000041
TCCACGG302.1644792E-645.0000041
ATGACGG600.045.0000041
GCGATAG551.8189894E-1245.0000048
TCTCCCG650.045.00000429
TTAGTCT1200.045.00000444
CGTTTTG600.045.00000429
Produced by FastQC (version 0.11.5)