##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043241_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	747653
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21367265295532	33.0	31.0	34.0	30.0	34.0
2	32.51284218748537	34.0	31.0	34.0	31.0	34.0
3	32.575317694170955	34.0	31.0	34.0	31.0	34.0
4	36.1334108202602	37.0	35.0	37.0	35.0	37.0
5	36.0881451689487	37.0	35.0	37.0	35.0	37.0
6	36.3214084608769	37.0	37.0	37.0	35.0	37.0
7	36.26740479875022	37.0	37.0	37.0	35.0	37.0
8	36.171102102178416	37.0	37.0	37.0	35.0	37.0
9	37.70795810355874	39.0	38.0	39.0	35.0	39.0
10	37.53670218670961	39.0	37.0	39.0	35.0	39.0
11	37.23676491634488	39.0	37.0	39.0	35.0	39.0
12	37.20762038004261	39.0	37.0	39.0	34.0	39.0
13	37.23730259893293	39.0	37.0	39.0	34.0	39.0
14	38.64643223527492	40.0	38.0	41.0	35.0	41.0
15	38.76458464020074	40.0	38.0	41.0	35.0	41.0
16	38.80516496289054	40.0	38.0	41.0	35.0	41.0
17	38.767781310313744	40.0	38.0	41.0	35.0	41.0
18	38.53186170589832	40.0	38.0	41.0	35.0	41.0
19	38.26699016789875	40.0	37.0	41.0	35.0	41.0
20	37.98584637525697	40.0	35.0	41.0	34.0	41.0
21	37.913180312257154	40.0	35.0	41.0	34.0	41.0
22	37.872182683678126	40.0	35.0	41.0	34.0	41.0
23	37.82918947693649	40.0	35.0	41.0	34.0	41.0
24	37.74381832213607	39.0	35.0	41.0	34.0	41.0
25	37.701988756816334	39.0	35.0	41.0	34.0	41.0
26	37.69368142707914	39.0	35.0	41.0	34.0	41.0
27	37.681860435255395	40.0	35.0	41.0	34.0	41.0
28	37.597749223235915	39.0	35.0	41.0	34.0	41.0
29	37.59244328585587	39.0	35.0	41.0	34.0	41.0
30	37.472105375087104	39.0	35.0	41.0	34.0	41.0
31	37.31293394128025	39.0	35.0	41.0	33.0	41.0
32	37.14896215222837	39.0	35.0	41.0	33.0	41.0
33	36.90519398705014	39.0	35.0	41.0	33.0	41.0
34	36.64695253011758	39.0	35.0	41.0	32.0	41.0
35	36.539726316887645	39.0	35.0	41.0	31.0	41.0
36	36.38676230818307	39.0	35.0	41.0	31.0	41.0
37	36.36920068534467	39.0	35.0	41.0	31.0	41.0
38	36.26418539081633	39.0	35.0	41.0	31.0	41.0
39	36.129241773924534	39.0	35.0	41.0	30.0	41.0
40	36.07500672103235	38.0	35.0	40.0	30.0	41.0
41	36.05884681797572	39.0	35.0	41.0	30.0	41.0
42	35.9742581117176	38.0	35.0	40.0	30.0	41.0
43	35.864197695989986	38.0	35.0	40.0	30.0	41.0
44	35.816551261079674	38.0	35.0	40.0	30.0	41.0
45	35.79348039799212	38.0	35.0	40.0	30.0	41.0
46	35.68914055049602	38.0	35.0	40.0	30.0	41.0
47	35.55210104152595	38.0	35.0	40.0	29.0	41.0
48	35.51560817652039	38.0	35.0	40.0	29.0	41.0
49	35.46287649484454	38.0	35.0	40.0	28.0	41.0
50	35.36642265863977	38.0	35.0	40.0	28.0	41.0
51	34.99331775569683	37.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	0.0
14	5.0
15	6.0
16	22.0
17	42.0
18	96.0
19	220.0
20	404.0
21	708.0
22	1117.0
23	1866.0
24	3384.0
25	6211.0
26	9488.0
27	11115.0
28	10871.0
29	10185.0
30	11156.0
31	13090.0
32	16306.0
33	21707.0
34	41621.0
35	80494.0
36	74006.0
37	57675.0
38	102717.0
39	273105.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.096661151630503	19.31029501653842	25.408444826677616	28.18459900515346
2	32.528726561653606	20.140760486482364	26.566334917401523	20.76417803446251
3	21.844759534168926	21.570166908980504	37.35021460490361	19.234858951946958
4	22.726318225165954	24.60834103521286	34.487522955167705	18.177817784453485
5	19.459963378733182	30.353519614045553	30.73872505025727	19.447791956963993
6	73.63121662054456	1.7070753411007513	20.644737598859365	4.016970439495327
7	74.3308727444416	1.3194623709127096	17.298532875545206	7.0511320091004785
8	62.671319448995725	3.1009037615043344	25.101350492808827	9.126426296691111
9	34.04600797428754	19.507311546934204	28.963703750269175	17.48297672850908
10	26.580245113709168	19.044262512154702	41.69086461232684	12.684627761809288
11	24.144890744770635	16.25901320532386	39.44677544261843	20.14932060728707
12	17.266432422527565	22.734477090307937	41.74650539755742	18.252585089607077
13	12.504330217360193	26.015812148148942	40.74403500019394	20.735822634296927
14	10.996010181193682	22.45560440471716	45.5097485063258	21.03863690776336
15	11.310995876429306	17.94829954537733	48.62589998301351	22.114804595179848
16	11.874091323113797	24.226746899965626	39.17231656931758	24.72684520760299
17	14.64864047893876	21.877930002287158	41.38296776713261	22.09046175164147
18	16.66334516145859	28.085756360236637	38.01616525313214	17.23473322517264
19	17.18203498146868	23.61509951809195	35.62000018725264	23.582865313186733
20	13.443669723788975	30.29560504672622	34.05590561396798	22.204819615516826
21	14.437178744685033	24.214842981971582	35.2798691371532	26.06810913619018
22	11.653266956729928	27.47838903876531	32.153151261347176	28.715192743157587
23	11.815374244469025	23.18267966556678	43.93762881978672	21.064317270177476
24	16.49776032464258	22.261262912072848	39.14676995879105	22.09420680449353
25	12.66416372301054	26.411048975928676	37.218736499418846	23.706050801641936
26	13.975199725006119	23.672077822198265	36.27351190993683	26.07921054285879
27	18.542291678091306	24.848425673407316	37.08418210051989	19.525100547981484
28	13.757986659586733	25.200728145275953	33.683941614626036	27.35734358051128
29	15.568585961669385	26.284385938396554	31.332315927308525	26.814712172625537
30	19.33169531855018	28.970792600310574	27.88312225056276	23.814389830576484
31	17.883028624241458	27.904522552574523	27.816513810551154	26.395935012632865
32	15.204647075581853	31.12887930630921	28.04335701187583	25.623116606233104
33	12.763541375477661	29.24177392453451	30.24237179547196	27.752312904515865
34	12.931935001932715	34.19380381005627	30.795302098700866	22.07895908931015
35	19.943342700423862	29.37726458664648	28.43217374905203	22.24721896387763
36	14.345959957359899	30.828873822481818	32.45556427915089	22.369601941007392
37	17.221090532640144	29.563982221699103	33.142513973728455	20.072413271932298
38	17.5576102817751	27.595154436616987	32.54129923908551	22.3059360425224
39	22.226621173191308	21.965804992422953	35.840155794198644	19.967418040187095
40	15.186590570759432	20.24481945501456	40.09868214265174	24.46990783157427
41	18.4731419522158	25.00692166018193	31.026960367978194	25.492976019624074
42	18.799630309782746	21.793666313115846	36.42117399381799	22.98552938328342
43	20.603274513711575	24.203206567752687	33.85501027883256	21.33850863970318
44	14.12179179378669	28.162396191816256	33.18411081076382	24.531701203633236
45	18.536941602588367	24.54360512162728	32.75088844691321	24.168564828871148
46	21.738426783547983	22.73915840637301	32.60964645363558	22.91276835644343
47	16.501371625607067	20.397831614398658	37.51887573513381	25.581921024860467
48	17.121579128285447	18.479562042819328	33.817961005974695	30.580897822920527
49	19.223222537728063	19.220279996201448	37.52369080308646	24.032806662984033
50	17.646154031348768	18.561150694239174	37.58668794213359	26.206007332278475
51	15.577279834361663	17.245968383728815	37.48182646227595	29.694925319633576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	1233.5
2	2257.0
3	27172.0
4	52087.0
5	28552.0
6	5017.0
7	4662.5
8	4308.0
9	4567.5
10	4827.0
11	4960.5
12	5094.0
13	4886.5
14	4679.0
15	4418.5
16	4158.0
17	3754.0
18	3350.0
19	3091.0
20	2832.0
21	2649.5
22	2467.0
23	2325.0
24	2183.0
25	2230.5
26	3026.0
27	3774.0
28	3847.0
29	3920.0
30	4971.0
31	6022.0
32	7077.5
33	8133.0
34	10123.5
35	12114.0
36	12952.5
37	13791.0
38	15732.5
39	17674.0
40	23128.5
41	28583.0
42	39143.5
43	49704.0
44	66161.5
45	82619.0
46	100876.0
47	119133.0
48	115725.5
49	112318.0
50	90101.5
51	67885.0
52	54234.0
53	40583.0
54	32208.0
55	23833.0
56	20761.5
57	17690.0
58	15804.5
59	13919.0
60	11609.5
61	9300.0
62	7632.0
63	5964.0
64	4946.5
65	3929.0
66	3429.5
67	2930.0
68	2484.5
69	2039.0
70	1806.0
71	1573.0
72	1724.5
73	1876.0
74	1617.5
75	969.5
76	580.0
77	491.0
78	402.0
79	280.5
80	159.0
81	99.0
82	39.0
83	35.0
84	31.0
85	20.0
86	9.0
87	9.5
88	10.0
89	8.5
90	7.0
91	4.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	747653.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.89801352622211
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82227143137024	22.809615412229174
2	11.587887729689324	7.160854236245679
3	4.429287495026721	4.105685547985862
4	2.2128460034917543	2.7349018298933903
5	1.297402692980845	2.004358297833957
6	0.8709487531452139	1.614635181319626
7	0.6137754331700604	1.327510914530595
8	0.4516041713788145	1.1162937436608749
9	0.3744035999663953	1.0411494746425138
>10	3.68205634911368	25.61201585579328
>50	0.47383062109456753	9.768641546538582
>100	0.1697238596523994	9.148917480741046
>500	0.008726162449994825	1.792306165275948
>1k	0.004799389347497155	3.289646863111221
>5k	0.0	0.0
>10k+	4.363081224997413E-4	6.473467450198251
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48019	6.42263188939254	No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	4960	0.6634093623646263	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	4378	0.5855657637968417	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	3622	0.48444933679126545	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	2252	0.3012092508155521	No Hit
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1758	0.23513581835423653	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCC	1515	0.20263410967387277	No Hit
ACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	1439	0.19246896621828577	No Hit
GCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	1296	0.17334244629527334	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	1096	0.14659206878057066	No Hit
CGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	1061	0.1419107527154977	No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	1025	0.13709568476285122	No Hit
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	936	0.12519176676880853	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	903	0.12077795447888258	No Hit
TGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT	848	0.11342160066233935	Illumina Single End Adapter 1 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	819	0.10954279592270746	No Hit
TGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	771	0.10312270531917882	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6108448705482357	0.0
2	0.0	0.0	0.0	2.1821620457618707	0.0
3	0.0	0.0	0.0	3.6571778619225763	0.0
4	0.0	0.0	0.0	4.661119530049368	0.0
5	0.0	0.0	0.0	9.454653428796513	0.0
6	0.0	0.0	0.0	10.821062712247526	0.0
7	0.0	0.0	0.0	15.06514385684268	0.0
8	0.0	0.0	0.0	20.120831455233912	0.0
9	0.0	0.0	0.0	23.966599478635143	0.0
10	0.0	0.0	0.0	26.197580963361347	0.0
11	0.0	0.0	0.0	28.191286599532138	0.0
12	0.0	0.0	0.0	29.59434390017829	0.0
13	0.0	0.0	0.0	30.196762401809394	0.0
14	0.0	0.0	0.0	30.61874960710383	0.0
15	0.0	0.0	0.0	31.976197514087417	0.0
16	0.0	0.0	0.0	33.59834040657899	0.0
17	0.0	0.0	0.0	35.3515601488926	0.0
18	0.0	0.0	0.0	36.25130909659963	0.0
19	0.0	0.0	0.0	37.159618165111354	0.0
20	1.3375188757351337E-4	0.0	0.0	38.19044396264042	0.0
21	0.0012037669881616204	0.0	0.0	39.22086850450677	0.0
22	0.0018725264260291874	0.0	0.0	40.25156055014826	0.0
23	0.0018725264260291874	0.0	0.0	41.03681788209236	0.0
24	0.002808789639043781	0.0	0.0	41.662509212161254	0.0
25	0.002808789639043781	0.0	0.0	42.218515808804355	0.0
26	0.002808789639043781	0.0	0.0	42.811839182080455	0.0
27	0.002808789639043781	0.0	0.0	43.39713744210215	0.0
28	0.002808789639043781	0.0	0.0	43.99514213144333	0.0
29	0.002808789639043781	0.0	0.0	44.7264974526953	0.0
30	0.002808789639043781	0.0	0.0	45.36342394132037	0.0
31	0.002808789639043781	0.0	0.0	45.881980009442884	0.0
32	0.002808789639043781	0.0	0.0	46.418860086162965	0.0
33	0.002808789639043781	0.0	0.0	46.935008620309155	0.0
34	0.002808789639043781	0.0	0.0	47.54959854370945	0.0
35	0.002808789639043781	0.0	0.0	48.03953170789123	0.0
36	0.002808789639043781	0.0	0.0	48.51582217954051	0.0
37	0.002808789639043781	0.0	0.0	48.99893399745604	0.0
38	0.002808789639043781	0.0	0.0	49.473619446454435	0.0
39	0.002808789639043781	0.0	0.0	49.932120917056444	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	120	0.0	45.000004	24
TACCCGG	30	2.1644792E-6	45.000004	1
ACCGGCG	30	2.1644792E-6	45.000004	19
GTCGATT	30	2.1644792E-6	45.000004	19
CCCTCCG	60	0.0	45.000004	22
GGTCGAT	30	2.1644792E-6	45.000004	18
GACGTGT	60	0.0	45.000004	43
CGTGACA	30	2.1644792E-6	45.000004	26
ATTGTCG	35	1.2111741E-7	45.000004	4
TCGATGG	75	0.0	45.000004	1
ACGGGTC	60	0.0	45.000004	4
ATCCGTG	30	2.1644792E-6	45.000004	23
ACGGCCA	35	1.2111741E-7	45.000004	6
TATTCGG	30	2.1644792E-6	45.000004	1
TCCACGG	30	2.1644792E-6	45.000004	1
ATGACGG	60	0.0	45.000004	1
GCGATAG	55	1.8189894E-12	45.000004	8
TCTCCCG	65	0.0	45.000004	29
TTAGTCT	120	0.0	45.000004	44
CGTTTTG	60	0.0	45.000004	29
>>END_MODULE