FastQCFastQC Report
Thu 26 May 2016
SRR1043240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043240_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences656347
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479797.31000522589423No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT59260.9028760701275392No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT47360.7215695356267341Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT40130.6114143890350684No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC21850.33290317469265496No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG16970.25855225970408946No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT15430.23508906112163233No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC13390.20400794092149424No Hit
GCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC12570.1915145494684976No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC11530.17566927250372136No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT11510.17536455563901412No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT9980.15205371548891058No Hit
TGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC8470.1290475922035143No Hit
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC8250.12569570669173472No Hit
TCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC7490.11411646583285974No Hit
ATAGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT7480.11396410740050612No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTTG7260.11061222188872655Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT7060.10756505324165419No Hit
ATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC7040.10726033637694696No Hit
CGTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG6880.10482260145928907No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCA6810.10375609243281374No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC6630.10101364065044861No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.10040420692103416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAGG1050.046.0000041
CTAGCGG1050.046.0000041
TATCAAC501.6370905E-1146.09
TGTCAGG850.046.01
TGTCACC253.4155433E-546.030
AAGTAGG1200.046.01
AACCGCT206.3094654E-446.09
GGTACCA301.8601731E-646.07
ACCGGCC206.3094654E-446.020
AGATCAT253.4155433E-546.011
TCCGCAA253.4155433E-546.031
CTCGTAG206.3094654E-446.042
TACCAGG253.4155433E-546.01
CAACGGC206.3094654E-446.02
CTATCTA206.3094654E-446.037
CGCGGCT206.3094654E-446.03
TTAGCAC650.046.029
GCAACTA301.8601731E-646.08
GCAACAC206.3094654E-446.028
CGTTGAA206.3094654E-446.031
Produced by FastQC (version 0.11.5)