##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043240_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	656347
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23009322812476	33.0	31.0	34.0	30.0	34.0
2	32.54861071963458	34.0	31.0	34.0	31.0	34.0
3	32.60933469643344	34.0	31.0	34.0	31.0	34.0
4	36.13876653660335	37.0	35.0	37.0	35.0	37.0
5	36.02230984524954	37.0	35.0	37.0	35.0	37.0
6	36.280170397670744	37.0	37.0	37.0	35.0	37.0
7	36.21265885271053	37.0	37.0	37.0	35.0	37.0
8	36.190926445919615	37.0	37.0	37.0	35.0	37.0
9	37.766612782567755	39.0	38.0	39.0	35.0	39.0
10	37.599024601316074	39.0	37.0	39.0	35.0	39.0
11	37.25034928170617	39.0	37.0	39.0	35.0	39.0
12	37.206756487041154	39.0	37.0	39.0	34.0	39.0
13	37.228001346848544	39.0	37.0	39.0	34.0	39.0
14	38.61634166073738	40.0	38.0	41.0	35.0	41.0
15	38.725137770112454	40.0	38.0	41.0	35.0	41.0
16	38.73708114762466	40.0	38.0	41.0	35.0	41.0
17	38.73275416814581	40.0	38.0	41.0	35.0	41.0
18	38.45505654783217	40.0	37.0	41.0	35.0	41.0
19	38.18746181516789	40.0	37.0	41.0	35.0	41.0
20	37.872012822485665	40.0	35.0	41.0	34.0	41.0
21	37.82047910632638	40.0	35.0	41.0	34.0	41.0
22	37.768648291224004	39.0	35.0	41.0	34.0	41.0
23	37.775870080917564	39.0	35.0	41.0	34.0	41.0
24	37.68910652444515	39.0	35.0	41.0	34.0	41.0
25	37.595611772431354	39.0	35.0	41.0	34.0	41.0
26	37.5551758444847	39.0	35.0	41.0	34.0	41.0
27	37.57105311672027	39.0	35.0	41.0	34.0	41.0
28	37.495452862586404	39.0	35.0	41.0	34.0	41.0
29	37.493712929288925	39.0	35.0	41.0	34.0	41.0
30	37.287802031547336	39.0	35.0	41.0	33.0	41.0
31	37.20985698113955	39.0	35.0	41.0	33.0	41.0
32	36.979014149527615	39.0	35.0	41.0	33.0	41.0
33	36.76626235817334	39.0	35.0	41.0	32.0	41.0
34	36.618116636474305	39.0	35.0	41.0	31.0	41.0
35	36.49982402601063	39.0	35.0	41.0	31.0	41.0
36	36.47511605903585	39.0	35.0	41.0	31.0	41.0
37	36.4279611242224	39.0	35.0	41.0	31.0	41.0
38	36.33962218155945	39.0	35.0	41.0	31.0	41.0
39	36.30718811848001	39.0	35.0	41.0	31.0	41.0
40	36.20434922380996	39.0	35.0	41.0	31.0	41.0
41	36.20602211939721	39.0	35.0	41.0	31.0	41.0
42	36.09103263974696	39.0	35.0	41.0	31.0	41.0
43	35.96898439392577	38.0	35.0	41.0	30.0	41.0
44	35.81112429857987	38.0	35.0	40.0	30.0	41.0
45	35.6963664037468	38.0	35.0	40.0	29.0	41.0
46	35.63804969017913	38.0	35.0	40.0	29.0	41.0
47	35.48924882722097	38.0	35.0	40.0	29.0	41.0
48	35.42463818681277	37.0	35.0	40.0	29.0	41.0
49	35.270967948356585	37.0	35.0	40.0	28.0	41.0
50	34.868536003059354	37.0	35.0	39.0	28.0	41.0
51	34.70061263325649	36.0	35.0	39.0	27.0	40.0
52	34.32546960677812	36.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	8.0
16	18.0
17	37.0
18	123.0
19	219.0
20	429.0
21	680.0
22	1095.0
23	1899.0
24	3436.0
25	5987.0
26	8324.0
27	9040.0
28	8821.0
29	8836.0
30	9988.0
31	12439.0
32	15378.0
33	19924.0
34	36333.0
35	72919.0
36	71618.0
37	51467.0
38	89544.0
39	227570.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.425584332677683	20.15412579016892	23.94434651182987	28.475943365323523
2	33.98217711058327	20.02157395402127	26.654955381833084	19.34129355356237
3	21.88324163895013	21.72707424578767	37.845377521341604	18.54430659392059
4	22.551485723253094	24.13388040167777	35.1055158323265	18.209118042742634
5	18.724851336259633	30.188299786545837	31.600205379166812	19.48664349802772
6	71.17332752339844	1.7007771803634357	22.7032347218773	4.422660574360818
7	72.57380623359289	1.320338174776452	18.39636655610523	7.709489035525416
8	59.850658264606984	2.88140267267162	27.379419727674538	9.888519335046858
9	32.78783935936326	19.430727953353944	29.604766990631482	18.176665696651316
10	25.788797693902765	18.211251060795586	43.228658011691984	12.77129323360966
11	22.8674771119545	15.939586834403144	39.89688381298307	21.296052240659286
12	15.548178021686699	22.8464516482897	43.466489524595985	18.13888080542762
13	11.503366359562852	26.14120274793669	43.03805761281761	19.31737327968285
14	9.474561474342078	23.423737748477556	46.55022419543321	20.551476581747156
15	9.671256210510599	18.662536737427	50.81808860252275	20.848118449539648
16	10.202682422560018	25.29744174956235	39.241742553862515	25.258133274015115
17	12.968140328210534	22.40506926976127	41.900244840000795	22.726545562027404
18	14.688571746347586	28.908336596343094	39.56169526180511	16.84139639550421
19	15.381345538259488	23.72784517945538	35.6783835379761	25.21242574430903
20	11.631956876469307	31.158975359070734	35.41388929941022	21.795178465049737
21	13.687729204216673	24.347943999134607	36.24439511416979	25.71993168247893
22	10.7936807816597	27.390998968533413	32.3156805774994	29.49963967230748
23	10.466567227396483	23.04268930916116	44.94238565880548	21.54835780463688
24	16.252531054457474	21.218501798591294	39.63467495090249	22.894292196048738
25	12.138243947180378	27.118582091485145	37.44040880814569	23.302765153188787
26	15.124316862878933	23.861920599926563	36.050747546648346	24.96301499054616
27	20.716176047121415	24.60634390040634	37.05707499234399	17.620405060128256
28	13.683615526543125	25.084901736429053	33.539271147731306	27.692211589296516
29	21.748709143181884	24.154601148477862	30.310795966158143	23.785893742182108
30	23.25781941564447	26.914117075266592	27.65320783061399	22.17485567847495
31	24.37049304712294	26.739361953356987	27.097404269387994	21.792740730132078
32	21.834029865299907	27.154386323088243	26.403563968449618	24.60801984316223
33	21.649371445287326	29.91024564750048	28.019629860424438	20.420753046787752
34	21.197323976494143	27.322285315541933	28.85760123836934	22.62278946959459
35	22.61395268051808	29.013311556234733	26.943369894278486	21.4293658689687
36	24.097771453209962	29.420565645915957	26.998371288358143	19.48329161251594
37	22.887893141889883	27.78210306438515	31.936460439371245	17.393543354353717
38	18.96070218954303	30.78508776607496	27.687488477893552	22.566721566488457
39	25.01405506538462	26.537182313623735	30.548018045332725	17.900744575658912
40	17.729341339261094	22.92567803311358	37.23365841544183	22.111322212183495
41	20.693322282268372	27.366469260924482	28.28656183390798	23.653646622899167
42	19.515743958607263	22.5810432591297	35.25193228581833	22.651280496444716
43	23.739119703449546	23.89254464482964	32.452803166617656	19.915532485103153
44	17.854122895358703	25.849588708411865	32.337315474893614	23.958972921335818
45	21.792740730132078	22.821769582248415	32.50582390107672	22.879665786542787
46	25.402264351021643	21.652723330799102	31.119057449794084	21.82595486838517
47	17.36870892988008	20.97731840017552	37.06895895006757	24.585013719876834
48	19.336418083727054	18.181845883351336	31.566534165616662	30.915201867304948
49	21.28432064136806	18.038476598506584	37.77803509424131	22.89916766588405
50	19.156635133549784	17.265866988041388	38.060659986257264	25.51683789215156
51	16.93189730432226	16.19006409719249	36.56754734919181	30.310491249293435
52	15.741825589208148	16.422563064964113	45.89813010495972	21.937481240868017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245.0
1	1258.0
2	2271.0
3	26518.5
4	50766.0
5	27140.0
6	3514.0
7	3374.5
8	3235.0
9	3490.0
10	3745.0
11	4045.0
12	4345.0
13	4425.0
14	4138.5
15	3772.0
16	3449.5
17	3127.0
18	2794.0
19	2461.0
20	2347.0
21	2233.0
22	2114.5
23	1996.0
24	1996.5
25	1997.0
26	2569.5
27	3142.0
28	3215.5
29	3289.0
30	3435.5
31	3582.0
32	4705.0
33	5828.0
34	6442.0
35	7056.0
36	7930.5
37	8805.0
38	10088.5
39	12936.0
40	14500.0
41	19451.5
42	24403.0
43	33047.5
44	41692.0
45	55651.5
46	69611.0
47	84236.5
48	98862.0
49	101214.5
50	103567.0
51	86651.5
52	69736.0
53	53833.5
54	37931.0
55	29114.5
56	20298.0
57	16459.5
58	12621.0
59	10714.5
60	8808.0
61	7487.5
62	6167.0
63	5206.0
64	3710.5
65	3176.0
66	2673.0
67	2170.0
68	2018.0
69	1866.0
70	1718.5
71	1571.0
72	1576.0
73	1581.0
74	1321.0
75	1061.0
76	765.0
77	469.0
78	432.5
79	396.0
80	297.0
81	198.0
82	138.0
83	78.0
84	59.5
85	41.0
86	22.5
87	4.0
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	656347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.642124928832885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58346288979742	21.811701996755133
2	11.517784577698837	6.828232187110672
3	4.538645482510438	4.0360528920077225
4	2.3000467827373616	2.7271309630424403
5	1.3669749939992375	2.026002177335799
6	0.88366492866995	1.5716223726517715
7	0.6253631316175807	1.2975964451306146
8	0.49615944546849067	1.1765776213757957
9	0.39442407007151375	1.0522410803998692
>10	3.570376413047412	23.608430919230738
>50	0.5099358436611608	10.04176456547138
>100	0.19612917063887603	10.410933586989984
>500	0.011354846721198086	2.2465304235383456
>1k	0.004645164567762853	2.918164076448543
>5k	5.161293964180948E-4	0.9066289355685261
>10k+	5.161293964180948E-4	7.340389756942679
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47979	7.31000522589423	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	5926	0.9028760701275392	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT	4736	0.7215695356267341	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	4013	0.6114143890350684	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	2185	0.33290317469265496	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG	1697	0.25855225970408946	No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	1543	0.23508906112163233	No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1339	0.20400794092149424	No Hit
GCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1257	0.1915145494684976	No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1153	0.17566927250372136	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	1151	0.17536455563901412	No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	998	0.15205371548891058	No Hit
TGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	847	0.1290475922035143	No Hit
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	825	0.12569570669173472	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	749	0.11411646583285974	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	748	0.11396410740050612	No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTTG	726	0.11061222188872655	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT	706	0.10756505324165419	No Hit
ATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	704	0.10726033637694696	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	688	0.10482260145928907	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCA	681	0.10375609243281374	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC	663	0.10101364065044861	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	659	0.10040420692103416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7467086769650809	0.0
2	0.0	0.0	0.0	2.7157890567032377	0.0
3	0.0	0.0	0.0	4.417175670796088	0.0
4	0.0	0.0	0.0	5.513394591580369	0.0
5	0.0	0.0	0.0	11.441661194459638	0.0
6	0.0	0.0	0.0	12.856156880430627	0.0
7	0.0	0.0	0.0	17.45936219713048	0.0
8	0.0	0.0	0.0	23.206017548644237	0.0
9	0.0	0.0	0.0	27.136712744935224	0.0
10	0.0	0.0	0.0	29.687192902534786	0.0
11	0.0	0.0	0.0	31.964189674059607	0.0
12	0.0	0.0	0.0	33.55496406626373	0.0
13	0.0	0.0	0.0	34.19806900922835	0.0
14	0.0	0.0	0.0	34.63015752338321	0.0
15	0.0	0.0	0.0	36.09995932029856	0.0
16	0.0	0.0	0.0	37.814905834870885	0.0
17	0.0	0.0	0.0	39.777282443585484	0.0
18	0.0	0.0	0.0	40.72876085363382	0.0
19	1.5235843235361784E-4	0.0	0.0	41.731888772250045	0.0
20	1.5235843235361784E-4	0.0	0.0	42.79108459397239	0.0
21	4.570752970608535E-4	0.0	0.0	43.75231394369137	0.0
22	6.094337294144714E-4	0.0	0.0	44.77159185613707	0.0
23	6.094337294144714E-4	0.0	0.0	45.51723402407568	0.0
24	0.0010665090264753247	0.0	0.0	46.115088512631274	0.0
25	0.0010665090264753247	0.0	0.0	46.66312179380724	0.0
26	0.0010665090264753247	0.0	0.0	47.20109941844786	0.0
27	0.0012188674588289427	0.0	0.0	47.72993553714727	0.0
28	0.0013712258911825605	0.0	0.0	48.25587684563196	0.0
29	0.0013712258911825605	0.0	0.0	48.95504969170271	0.0
30	0.0013712258911825605	0.0	0.0	49.53370701778175	0.0
31	0.0013712258911825605	0.0	0.0	50.00114268824265	0.0
32	0.0013712258911825605	0.0	0.0	50.462941096706466	0.0
33	0.0013712258911825605	0.0	0.0	50.91255083058199	0.0
34	0.0013712258911825605	0.0	0.0	51.4919699488228	0.0
35	0.0013712258911825605	0.0	0.0	51.94005609837479	0.0
36	0.0013712258911825605	0.0	0.0	52.352338016323685	0.0
37	0.0013712258911825605	0.0	0.0	52.79356803641976	0.0
38	0.0013712258911825605	0.0	0.0	53.23129381257171	0.0
39	0.0013712258911825605	0.0	0.0	53.6563738388383	0.0
40	0.0015235843235361782	0.0	0.0	54.075207169378395	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGAGG	105	0.0	46.000004	1
CTAGCGG	105	0.0	46.000004	1
TATCAAC	50	1.6370905E-11	46.0	9
TGTCAGG	85	0.0	46.0	1
TGTCACC	25	3.4155433E-5	46.0	30
AAGTAGG	120	0.0	46.0	1
AACCGCT	20	6.3094654E-4	46.0	9
GGTACCA	30	1.8601731E-6	46.0	7
ACCGGCC	20	6.3094654E-4	46.0	20
AGATCAT	25	3.4155433E-5	46.0	11
TCCGCAA	25	3.4155433E-5	46.0	31
CTCGTAG	20	6.3094654E-4	46.0	42
TACCAGG	25	3.4155433E-5	46.0	1
CAACGGC	20	6.3094654E-4	46.0	2
CTATCTA	20	6.3094654E-4	46.0	37
CGCGGCT	20	6.3094654E-4	46.0	3
TTAGCAC	65	0.0	46.0	29
GCAACTA	30	1.8601731E-6	46.0	8
GCAACAC	20	6.3094654E-4	46.0	28
CGTTGAA	20	6.3094654E-4	46.0	31
>>END_MODULE