FastQCFastQC Report
Thu 26 May 2016
SRR1043239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043239_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences638112
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436816.845350032596159No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC54630.8561193019407252No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT45550.7138245323704929No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC39520.6193270146933454No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG20430.3201632315330224No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC15290.2396131086705782No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC14820.2322476305100045No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG12740.19965147184193371No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC11810.1850772278220751No Hit
GCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG11640.1824131187001655No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG11500.18021914648212226No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC9290.145585727897297No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT8460.13257860689032647Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG8200.1285040870568176No Hit
TGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG7860.12317586881299834No Hit
TCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG7720.12098189659495512No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC6880.10781806328669576No Hit
CGTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT6860.10750463868411815No Hit
ATAGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC6670.1045271049596309No Hit
CGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT6640.1040569680557645No Hit
ATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG6430.10076600972869967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACC302.1639771E-645.00000430
GCCCATC302.1639771E-645.0000048
TCGCAGG650.045.0000041
AAATCCG302.1639771E-645.00000443
GTACAGG1400.045.0000041
TTAGCAC600.045.00000429
GTTTAGA351.2107921E-745.00000430
TAGGTTG302.1639771E-645.00000435
AGGGTGC351.2107921E-745.0000045
TACGGGA1200.045.0000043
CCTGTCA302.1639771E-645.00000428
TCGATGG351.2107921E-745.0000041
CCTCGCG351.2107921E-745.00000426
TAGCCGG302.1639771E-645.0000041
ATGTAGA650.045.00000439
GCATTAC351.2107921E-745.0000048
CTTATGG600.045.0000041
CTACGGC302.1639771E-645.0000042
CTTGCGG1400.045.0000041
GTCACGG302.1639771E-645.0000041