##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043239_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	638112
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.165130886114035	33.0	31.0	34.0	30.0	34.0
2	32.458372511408655	34.0	31.0	34.0	31.0	34.0
3	32.508393510857026	34.0	31.0	34.0	31.0	34.0
4	36.09017382528459	37.0	35.0	37.0	35.0	37.0
5	36.0343387994584	37.0	35.0	37.0	35.0	37.0
6	36.28754513314277	37.0	37.0	37.0	35.0	37.0
7	36.23185584975678	37.0	37.0	37.0	35.0	37.0
8	36.1382782332882	37.0	37.0	37.0	35.0	37.0
9	37.71049909733714	39.0	38.0	39.0	35.0	39.0
10	37.53175461361015	39.0	37.0	39.0	35.0	39.0
11	37.230153954164784	39.0	37.0	39.0	34.0	39.0
12	37.189675166741885	39.0	37.0	39.0	34.0	39.0
13	37.23220218394263	39.0	37.0	39.0	34.0	39.0
14	38.622603868913295	40.0	38.0	41.0	35.0	41.0
15	38.74040920716113	40.0	38.0	41.0	35.0	41.0
16	38.78135656436488	40.0	38.0	41.0	35.0	41.0
17	38.75488942380021	40.0	38.0	41.0	35.0	41.0
18	38.511874091068655	40.0	38.0	41.0	35.0	41.0
19	38.27259164535379	40.0	37.0	41.0	35.0	41.0
20	37.98321924677799	40.0	35.0	41.0	34.0	41.0
21	37.92664767313575	40.0	35.0	41.0	34.0	41.0
22	37.887413181385085	40.0	35.0	41.0	34.0	41.0
23	37.87013721729101	40.0	35.0	41.0	34.0	41.0
24	37.783633594102604	40.0	35.0	41.0	34.0	41.0
25	37.75505083747054	40.0	35.0	41.0	34.0	41.0
26	37.73808202948699	40.0	35.0	41.0	34.0	41.0
27	37.718883205456095	40.0	35.0	41.0	34.0	41.0
28	37.63286382327867	40.0	35.0	41.0	34.0	41.0
29	37.65101424201394	40.0	35.0	41.0	34.0	41.0
30	37.38185616318138	39.0	35.0	41.0	33.0	41.0
31	37.29187039265834	39.0	35.0	41.0	33.0	41.0
32	37.08215955819668	39.0	35.0	41.0	33.0	41.0
33	36.82669970161978	39.0	35.0	41.0	32.0	41.0
34	36.62708113936112	39.0	35.0	41.0	31.0	41.0
35	36.51140865553383	39.0	35.0	41.0	31.0	41.0
36	36.469729451883055	39.0	35.0	41.0	31.0	41.0
37	36.37048981996891	39.0	35.0	41.0	31.0	41.0
38	36.28043666315631	39.0	35.0	41.0	31.0	41.0
39	36.20200529060729	39.0	35.0	41.0	31.0	41.0
40	36.071568940875586	38.0	35.0	41.0	30.0	41.0
41	36.023102527456	38.0	35.0	41.0	30.0	41.0
42	35.89004281380071	38.0	35.0	40.0	30.0	41.0
43	35.76027875984153	38.0	35.0	40.0	29.0	41.0
44	35.63958051251191	38.0	35.0	40.0	29.0	41.0
45	35.571880798355146	38.0	35.0	40.0	29.0	41.0
46	35.45547646808084	38.0	35.0	40.0	28.0	41.0
47	35.24981821373051	37.0	35.0	40.0	28.0	41.0
48	35.21847418634973	37.0	35.0	40.0	28.0	41.0
49	35.0572470036608	37.0	35.0	40.0	28.0	41.0
50	34.70100703324808	36.0	35.0	39.0	27.0	40.0
51	34.234118775387394	36.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	4.0
16	13.0
17	33.0
18	78.0
19	216.0
20	373.0
21	631.0
22	1008.0
23	1577.0
24	2793.0
25	5092.0
26	7652.0
27	9161.0
28	9087.0
29	9114.0
30	10074.0
31	12024.0
32	14602.0
33	19376.0
34	36252.0
35	70810.0
36	66698.0
37	50204.0
38	91710.0
39	219506.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.825522792237102	20.202879745248485	24.05925605536332	27.912341407151096
2	33.51214206910386	20.180469886164186	26.8263251592197	19.481062885512262
3	22.137179680056164	21.857761646858233	37.26555839727195	18.73950027581365
4	22.806184494258062	24.390545860287848	34.425147936412415	18.37812170904167
5	18.952315580963845	30.536175467629505	30.925448824030894	19.58606012737576
6	72.03563010882102	1.6005027330625345	22.004601073165837	4.359266084950604
7	73.31816358256857	1.2499373150794846	17.69187854169801	7.740020560653929
8	60.48279925781054	2.768166089965398	26.778841331929193	9.97019332029487
9	33.19840404192367	19.545941778245826	29.00917080387142	18.24648337595908
10	26.1041948748809	18.31857103455193	42.79374153753573	12.783492553031442
11	23.100646908379723	15.789547916353241	39.6300335991174	21.47977157614964
12	15.776384083044984	22.747417381274758	43.04542149340554	18.43077704227471
13	11.637612206007724	26.09729953362419	42.71052730555137	19.55456095481671
14	9.584524346823128	23.243881951757686	46.332462012938166	20.839131688481018
15	9.792951707537236	18.4882904568477	50.60240208615415	21.11635574946091
16	10.24224587533223	25.195420239707133	39.05239205656687	25.50994182839376
17	13.1605423499323	22.291071159921767	41.68014392457751	22.868242565568426
18	14.93311518980994	28.83663056015245	39.352339401233635	16.87791484880397
19	15.710721628804974	23.681736121558597	35.296781756180735	25.310760493455692
20	11.757340404192368	31.260499724186353	34.95060428263377	22.031555588987516
21	13.765921969810943	24.37095682262675	35.78901509452886	26.074106113033444
22	10.820514267087908	27.350214382428163	31.94580261772228	29.88346873276165
23	10.699845795095532	23.07604307707738	44.44219823479264	21.781912893034452
24	16.288206459054212	21.13986259465423	39.42035253999298	23.151578406298583
25	12.11151020510506	27.059669775838724	37.25004387944436	23.578776139611854
26	15.203914046436989	23.77184569479966	35.83226768968457	25.19197256907878
27	20.906361265733914	24.602107467027732	36.598277418384235	17.893253848854123
28	13.752914848803972	25.015671230128877	33.23993280176521	27.99148111930194
29	21.978430118850607	24.088874680306905	29.937064339802415	23.99563086104007
30	23.45889122912592	26.928658291961288	27.12392181936713	22.488528659545658
31	24.564183090115844	26.586241913645253	26.63623313775638	22.213341858482526
32	22.087345168246326	26.99463417080387	25.99010831954265	24.92791234140715
33	21.896783009879144	29.781762449225212	27.552529963392008	20.768924577503636
34	21.39263953663307	27.32357329120907	28.45958076325159	22.82420640890627
35	22.86150393661301	28.97218670076726	26.613509854069505	21.552799508550223
36	24.467805024823228	29.43339100346021	26.424984955619074	19.67381901609749
37	23.271620029085803	27.69075021312873	31.523462965748962	17.51416679203651
38	19.241136352239106	30.86166691740635	27.180965097036257	22.71623163331829
39	25.233501328920315	26.647829848051753	30.07669500025074	18.041973822777194
40	17.885261521488392	22.828907777944938	37.027042274710396	22.258788425856277
41	20.90307030740685	27.50097161626799	27.883349631412667	23.71260844491249
42	19.69951983350885	22.587727546261473	34.860808133995285	22.85194448623439
43	23.891260468381724	23.920879093325308	32.1399691590191	20.04789127927386
44	17.981482874479717	25.756606990622338	32.1442003911539	24.117709743744044
45	22.179178576801565	22.654957123514368	32.205318188656534	22.96054611102753
46	25.779016849706633	21.58602251642345	30.706521739130434	21.928438894739482
47	17.807532220049143	20.87423524396971	36.595456596961036	24.72277593902011
48	19.518673837821574	18.01454917005165	31.248119452384532	31.21865753974224
49	21.536501429216187	17.760518529662505	37.48166466074921	23.221315380372097
50	19.459436587934405	17.190085752971264	37.70513640238704	25.645341256707287
51	17.20654054460659	16.03731006469084	36.156818865653676	30.599330525048895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	180.0
1	1110.5
2	2041.0
3	24163.0
4	46285.0
5	25043.5
6	3802.0
7	3485.5
8	3169.0
9	3447.0
10	3725.0
11	3870.0
12	4015.0
13	3963.5
14	3912.0
15	3590.5
16	3269.0
17	3013.5
18	2758.0
19	2482.5
20	2207.0
21	2091.0
22	1975.0
23	1876.5
24	1778.0
25	1885.0
26	2576.0
27	3160.0
28	3008.0
29	2856.0
30	3643.0
31	4430.0
32	4833.0
33	5236.0
34	6466.0
35	7696.0
36	9070.5
37	10445.0
38	11076.0
39	11707.0
40	15543.5
41	19380.0
42	24741.0
43	30102.0
44	37507.0
45	44912.0
46	59763.5
47	74615.0
48	90812.0
49	107009.0
50	103883.5
51	100758.0
52	77773.5
53	54789.0
54	41321.5
55	27854.0
56	21839.0
57	15824.0
58	13206.5
59	10589.0
60	8696.0
61	6803.0
62	5769.0
63	4735.0
64	4212.5
65	3690.0
66	3084.5
67	2479.0
68	2244.0
69	2009.0
70	1911.5
71	1814.0
72	1668.5
73	1523.0
74	1362.0
75	889.0
76	577.0
77	482.0
78	387.0
79	322.5
80	258.0
81	182.0
82	106.0
83	76.5
84	47.0
85	25.5
86	4.0
87	4.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	638112.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.430675492458157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.00206606544465	21.48500116651093
2	11.746234787800125	6.913992485959371
3	4.648774450079819	4.104497168337893
4	2.3054588287730953	2.7140484260337447
5	1.3905390297011524	2.046225147136612
6	0.9137069460703044	1.6134607575000057
7	0.6348349548665218	1.3078535083562142
8	0.4927735833370056	1.1602127537957756
9	0.3920946098839938	1.0385648303254016
>10	3.76384900100692	24.695375733018317
>50	0.49730504720356883	9.733984081556041
>100	0.1962338834911328	10.509772438841695
>500	0.010214914483100065	2.0007839644869656
>1k	0.004838643702521083	2.9003060552824103
>5k	5.376270780578982E-4	0.8643956344794222
>10k+	5.376270780578982E-4	6.911525848379213
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43681	6.845350032596159	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	5463	0.8561193019407252	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	4555	0.7138245323704929	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	3952	0.6193270146933454	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	2043	0.3201632315330224	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC	1529	0.2396131086705782	No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1482	0.2322476305100045	No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	1274	0.19965147184193371	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	1181	0.1850772278220751	No Hit
GCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	1164	0.1824131187001655	No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	1150	0.18021914648212226	No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	929	0.145585727897297	No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT	846	0.13257860689032647	Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	820	0.1285040870568176	No Hit
TGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	786	0.12317586881299834	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	772	0.12098189659495512	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	688	0.10781806328669576	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	686	0.10750463868411815	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	667	0.1045271049596309	No Hit
CGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	664	0.1040569680557645	No Hit
ATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	643	0.10076600972869967	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7388985005767013	0.0
2	0.0	0.0	0.0	2.658310766761948	0.0
3	0.0	0.0	0.0	4.347512662353944	0.0
4	0.0	0.0	0.0	5.439640690035605	0.0
5	0.0	0.0	0.0	11.36070156963041	0.0
6	0.0	0.0	0.0	12.78004488240309	0.0
7	0.0	0.0	0.0	17.467466526252444	0.0
8	0.0	0.0	0.0	23.35953563010882	0.0
9	0.0	0.0	0.0	27.39221327917356	0.0
10	0.0	0.0	0.0	30.02059199638935	0.0
11	0.0	0.0	0.0	32.343068301489396	0.0
12	0.0	0.0	0.0	33.972562810290356	0.0
13	0.0	0.0	0.0	34.62573968206208	0.0
14	0.0	0.0	0.0	35.081615766511206	0.0
15	0.0	0.0	0.0	36.586210571185	0.0
16	0.0	0.0	0.0	38.36301464319743	0.0
17	0.0	0.0	0.0	40.37849155007272	0.0
18	0.0	0.0	0.0	41.355279324005814	0.0
19	0.0	0.0	0.0	42.39459530615315	0.0
20	0.0	0.0	0.0	43.435321699012086	0.0
21	1.5671230128880197E-4	0.0	0.0	44.43514618123464	0.0
22	3.1342460257760394E-4	0.0	0.0	45.48010380622837	0.0
23	4.701369038664059E-4	0.0	0.0	46.249247780953816	0.0
24	0.0015671230128880196	0.0	0.0	46.870141918660046	0.0
25	0.0015671230128880196	0.0	0.0	47.43493305250489	0.0
26	0.0015671230128880196	0.0	0.0	47.9763740534577	0.0
27	0.0015671230128880196	0.0	0.0	48.52424025876335	0.0
28	0.0015671230128880196	0.0	0.0	49.0567486585427	0.0
29	0.0015671230128880196	0.0	0.0	49.75411839927787	0.0
30	0.0015671230128880196	0.0	0.0	50.37626623539441	0.0
31	0.0015671230128880196	0.0	0.0	50.84420916704278	0.0
32	0.0015671230128880196	0.0	0.0	51.32562935660198	0.0
33	0.0015671230128880196	0.0	0.0	51.80219146482122	0.0
34	0.0015671230128880196	0.0	0.0	52.38061656887819	0.0
35	0.0015671230128880196	0.0	0.0	52.81768717717266	0.0
36	0.0015671230128880196	0.0	0.0	53.23250463868412	0.0
37	0.0015671230128880196	0.0	0.0	53.677410862043025	0.0
38	0.0015671230128880196	0.0	0.0	54.12686174213931	0.0
39	0.0015671230128880196	0.0	0.0	54.56252193972218	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACC	30	2.1639771E-6	45.000004	30
GCCCATC	30	2.1639771E-6	45.000004	8
TCGCAGG	65	0.0	45.000004	1
AAATCCG	30	2.1639771E-6	45.000004	43
GTACAGG	140	0.0	45.000004	1
TTAGCAC	60	0.0	45.000004	29
GTTTAGA	35	1.2107921E-7	45.000004	30
TAGGTTG	30	2.1639771E-6	45.000004	35
AGGGTGC	35	1.2107921E-7	45.000004	5
TACGGGA	120	0.0	45.000004	3
CCTGTCA	30	2.1639771E-6	45.000004	28
TCGATGG	35	1.2107921E-7	45.000004	1
CCTCGCG	35	1.2107921E-7	45.000004	26
TAGCCGG	30	2.1639771E-6	45.000004	1
ATGTAGA	65	0.0	45.000004	39
GCATTAC	35	1.2107921E-7	45.000004	8
CTTATGG	60	0.0	45.000004	1
CTACGGC	30	2.1639771E-6	45.000004	2
CTTGCGG	140	0.0	45.000004	1
GTCACGG	30	2.1639771E-6	45.000004	1
>>END_MODULE