FastQCFastQC Report
Thu 26 May 2016
SRR1043238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043238_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1087923
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT544105.001273068038822No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT74340.6833204188164052TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT67540.6208159952496638TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT49770.4574772295465763TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC30090.27658207428283066TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT27790.2554408721940799TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG24910.2289684104481659No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC20330.18686984281056654TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC18030.16572864072181578TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC15980.14688539538184228TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT14720.1353036933680049TruSeq Adapter, Index 22 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC13700.1259280298329937TruSeq Adapter, Index 22 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT13490.1239977461640208No Hit
GGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC11120.10221311618561241TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA206.311306E-446.021
TACCCGC253.417035E-546.030
ACTGCGG1050.046.01
ACGCCCG206.311306E-446.014
GATCGTC206.311306E-446.09
GCGCGTT206.311306E-446.08
CCGTGCT206.311306E-446.043
GCGCCCT206.311306E-446.08
CGATTCG206.311306E-446.09
TGCGGAA206.311306E-446.03
GTAATAG206.311306E-446.012
ATATCGG405.6115823E-946.01
CTAACGG1300.046.01
CACTCCG301.86131E-646.017
ACGGCCA301.86131E-646.06
TGCGATT206.311306E-446.018
TTGCGCT253.417035E-546.02
GAACGAT206.311306E-446.08
CGTAAAC206.311306E-446.023
TTACGGC501.6370905E-1146.02
Produced by FastQC (version 0.11.5)