##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043238_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1087923
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28634471373434	33.0	31.0	34.0	31.0	34.0
2	32.61847575609671	34.0	31.0	34.0	31.0	34.0
3	32.70460685177168	34.0	31.0	34.0	31.0	34.0
4	36.2076599171081	37.0	37.0	37.0	35.0	37.0
5	36.11030468148941	37.0	35.0	37.0	35.0	37.0
6	36.34405651870583	37.0	37.0	37.0	35.0	37.0
7	36.282116473316584	37.0	37.0	37.0	35.0	37.0
8	36.25607970417024	37.0	37.0	37.0	35.0	37.0
9	37.80626570078949	39.0	38.0	39.0	35.0	39.0
10	37.586363189306596	39.0	37.0	39.0	35.0	39.0
11	37.35336232435568	39.0	37.0	39.0	35.0	39.0
12	37.37139852728548	39.0	37.0	39.0	34.0	39.0
13	37.379993804708604	39.0	37.0	39.0	35.0	39.0
14	38.78947590960022	40.0	38.0	41.0	35.0	41.0
15	38.87900246616718	41.0	38.0	41.0	35.0	41.0
16	38.89732177736843	41.0	38.0	41.0	35.0	41.0
17	38.88462786428819	41.0	38.0	41.0	35.0	41.0
18	38.63863894779318	40.0	38.0	41.0	35.0	41.0
19	38.385256125663304	40.0	37.0	41.0	35.0	41.0
20	38.10612607693743	40.0	35.0	41.0	34.0	41.0
21	38.0415268359985	40.0	35.0	41.0	34.0	41.0
22	37.99734631954651	40.0	35.0	41.0	34.0	41.0
23	37.99001675670061	40.0	35.0	41.0	34.0	41.0
24	37.919838076775655	40.0	35.0	41.0	34.0	41.0
25	37.839122805566205	40.0	35.0	41.0	34.0	41.0
26	37.79737260817172	40.0	35.0	41.0	34.0	41.0
27	37.82337628674088	40.0	35.0	41.0	34.0	41.0
28	37.82251501255144	40.0	35.0	41.0	34.0	41.0
29	37.74051104719727	40.0	35.0	41.0	34.0	41.0
30	37.635570715942215	40.0	35.0	41.0	34.0	41.0
31	37.44870914577594	40.0	35.0	41.0	33.0	41.0
32	37.2240149348805	40.0	35.0	41.0	33.0	41.0
33	36.9699381298125	40.0	35.0	41.0	32.0	41.0
34	36.658383911361376	40.0	35.0	41.0	31.0	41.0
35	36.575220856623126	40.0	35.0	41.0	31.0	41.0
36	36.45694778031166	39.0	35.0	41.0	30.0	41.0
37	36.349833581972256	39.0	35.0	41.0	30.0	41.0
38	36.33166685509912	39.0	35.0	41.0	30.0	41.0
39	36.35789113751617	39.0	35.0	41.0	30.0	41.0
40	36.326372362749936	39.0	35.0	41.0	30.0	41.0
41	36.31975332813076	39.0	35.0	41.0	30.0	41.0
42	36.271751769196904	39.0	35.0	41.0	30.0	41.0
43	36.197506625009304	39.0	35.0	41.0	30.0	41.0
44	36.01270218572454	39.0	35.0	41.0	30.0	41.0
45	35.92659774634786	39.0	35.0	41.0	29.0	41.0
46	35.89524902038104	39.0	35.0	41.0	29.0	41.0
47	35.827579709225745	39.0	35.0	41.0	29.0	41.0
48	35.8071775300274	39.0	35.0	41.0	29.0	41.0
49	35.7718469046063	39.0	35.0	41.0	28.0	41.0
50	35.641435101565094	39.0	35.0	40.0	28.0	41.0
51	35.567819597526665	39.0	35.0	40.0	27.0	41.0
52	35.21208945853705	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	2.0
14	4.0
15	5.0
16	26.0
17	54.0
18	164.0
19	317.0
20	589.0
21	1042.0
22	1638.0
23	3105.0
24	5684.0
25	10745.0
26	15567.0
27	16704.0
28	15243.0
29	14446.0
30	15800.0
31	18600.0
32	23112.0
33	30122.0
34	55108.0
35	97648.0
36	97120.0
37	83706.0
38	142471.0
39	438314.0
40	585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.486871773094236	19.659939168488947	26.308203797511403	26.544985260905413
2	33.326255626547095	20.04985646962147	27.20587762185375	19.418010281977676
3	21.743266756930407	21.909546907271928	36.68743100384862	19.659755331949043
4	23.2304124464691	24.4743423937172	33.829232399719466	18.466012760094237
5	20.553568588953446	30.222175650298784	30.375127651497397	18.84912810925038
6	75.04253517941987	1.5193170840215713	19.809122520619564	3.6290252159389955
7	76.21734258766475	1.2690236349447526	16.246186540775405	6.267447236615091
8	64.99880965840413	3.4029062718593135	23.610494492716853	7.987789577019696
9	32.470220778492596	25.719834951554475	26.171889003173938	15.638055266778991
10	23.917961105703252	19.705990221734442	43.791794088368384	12.584254584193918
11	21.79906114679072	17.709709234936664	40.2475175173243	20.243712100948322
12	17.210776865642146	21.87645633008954	43.310234272094625	17.602532532173694
13	13.588737438219434	24.83925792542303	42.14140155139656	19.430603084960975
14	12.361720452642329	24.07008584247231	43.715961515658734	19.852232189226626
15	13.315556339924791	20.475346141225067	46.65762190890348	19.55147560994666
16	13.273182017477339	24.868212180457625	39.12786107105006	22.73074473101497
17	14.597264696122794	22.187232000794175	40.97109813837928	22.24440516470375
18	16.120442347482314	27.39679186854217	39.71209359485919	16.770672189116326
19	16.57120954332246	23.562421237532437	36.96566760699057	22.900701612154535
20	14.372800280902235	29.017127131239988	36.23703148108828	20.373041106769506
21	15.207693926867986	23.685499800996944	37.28140686427256	23.825399407862506
22	12.89374339911924	26.08695652173913	33.66938652827452	27.34991355086711
23	12.445182241757918	22.894359251527913	43.40095760453635	21.25950090217782
24	16.687026563460833	22.199916722047426	39.086957440921836	22.026099273569912
25	13.122619891297454	27.074986005443403	37.616173203434435	22.18622089982471
26	14.436683478518242	24.493920985216786	37.22359027247333	23.845805263791647
27	18.528425265391025	25.809179509946933	37.46689793303386	18.195497291628175
28	14.219572524893763	25.455018415825386	35.08171074607302	25.24369831320783
29	17.471640915763338	28.37581336179123	32.26698948363074	21.885556238814697
30	20.843754567189038	27.44918528241429	30.662280326824597	21.044779823572075
31	18.908507311638783	29.064556958534748	31.115345479413527	20.911590250412942
32	19.451928123589628	28.8581085242246	29.897979912181285	21.791983440004486
33	17.327788823289882	29.279645710220297	30.87139439096333	22.521171075526485
34	16.992379056238356	26.909073528181683	34.76624724360088	21.33230017197908
35	22.76778779380526	25.474413170785066	29.067590261443133	22.69020877396654
36	21.392414720527096	24.86453544965958	31.39008918829733	22.35296064151599
37	18.9312111243167	23.77971602769681	36.32086094328367	20.968211904702812
38	17.50693753142456	22.267476650461475	31.53596348270971	28.689622335404252
39	22.06884126909717	20.59346111811222	32.63208885187647	24.705608760914146
40	15.627576584004565	20.550259531235206	37.16439490662483	26.657768978135397
41	17.495355829410723	25.64767910964287	29.936217912480938	26.92074714846547
42	19.119275904636634	21.788582464016297	34.050847348571544	25.041294282775528
43	23.257804090914522	23.749750671692755	31.812545556992543	21.179899680400176
44	18.187132729062625	25.379921189275343	33.05895729752933	23.373988784132703
45	20.181667268731335	22.743521370538172	33.26172900104144	23.81308235968906
46	22.737730519531254	20.948817149743135	32.60313459684187	23.71031773388374
47	16.88989018524289	20.056014993708192	36.34080720786306	26.71328761318586
48	18.707206300445893	18.04107459811034	32.05557746274323	31.19614163870053
49	20.90717817345529	18.183455998264584	36.64220721503268	24.267158613247446
50	19.061367394567448	17.386065006438876	37.1749655076692	26.377602091324476
51	17.09652245609294	17.134668538122643	35.28668848806396	30.482120517720464
52	16.122464549421238	17.054699643265195	42.947524778867624	23.875311028445946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	434.0
1	1551.5
2	2669.0
3	32350.5
4	62032.0
5	34691.5
6	7351.0
7	7051.0
8	6751.0
9	7016.5
10	7282.0
11	7525.5
12	7769.0
13	7692.5
14	7076.5
15	6537.0
16	6170.0
17	5803.0
18	5322.5
19	4842.0
20	4692.0
21	4542.0
22	4259.0
23	3976.0
24	4225.0
25	4474.0
26	5305.0
27	6136.0
28	6644.5
29	7153.0
30	8216.5
31	9280.0
32	11347.5
33	13415.0
34	15190.5
35	16966.0
36	19757.5
37	22549.0
38	25071.0
39	30927.5
40	34262.0
41	41856.5
42	49451.0
43	61878.0
44	74305.0
45	92464.0
46	110623.0
47	131935.5
48	153248.0
49	155410.0
50	157572.0
51	133771.0
52	109970.0
53	86801.5
54	63633.0
55	48473.5
56	33314.0
57	26930.0
58	20546.0
59	17167.5
60	13789.0
61	11801.0
62	9813.0
63	8284.0
64	5394.5
65	4034.0
66	3498.5
67	2963.0
68	2654.5
69	2346.0
70	2132.5
71	1919.0
72	1963.0
73	2007.0
74	1519.0
75	1031.0
76	757.0
77	483.0
78	455.0
79	427.0
80	277.0
81	127.0
82	93.5
83	60.0
84	53.0
85	46.0
86	32.0
87	18.0
88	13.0
89	4.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1087923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.57572786142969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78598781617137	22.666948155473346
2	12.231491428787342	7.724364893895978
3	5.013222150157767	4.748884149668191
4	2.596965772633828	3.28004338008533
5	1.5464359380198018	2.4414920167024006
6	0.9876417370877199	1.8711304028922922
7	0.7047584805320484	1.5577283392520256
8	0.5146940041399588	1.3001470245306326
9	0.4085937946051595	1.1611481817889273
>10	3.6426035121848206	25.07198274798003
>50	0.3971783668593117	8.302062281463325
>100	0.15671112734084225	9.157797200580331
>500	0.009046638635820276	1.995156364609103
>1k	0.0037937516859891476	2.400048155949044
>5k	5.836541055367919E-4	1.3073747691240512
>10k+	2.9182705276839597E-4	5.013691936005048
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54410	5.001273068038822	No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	7434	0.6833204188164052	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT	6754	0.6208159952496638	TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	4977	0.4574772295465763	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	3009	0.27658207428283066	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	2779	0.2554408721940799	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	2491	0.2289684104481659	No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2033	0.18686984281056654	TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	1803	0.16572864072181578	TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	1598	0.14688539538184228	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	1472	0.1353036933680049	TruSeq Adapter, Index 22 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	1370	0.1259280298329937	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	1349	0.1239977461640208	No Hit
GGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	1112	0.10221311618561241	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6396592405896373	0.0
2	0.0	0.0	0.0	2.215138387551325	0.0
3	0.0	0.0	0.0	3.682613567320481	0.0
4	0.0	0.0	0.0	4.578357107993856	0.0
5	0.0	0.0	0.0	9.133366975420136	0.0
6	0.0	0.0	0.0	10.125716617812106	0.0
7	0.0	0.0	0.0	13.67964460720106	0.0
8	0.0	0.0	0.0	18.14025441138757	0.0
9	0.0	0.0	0.0	21.34627174901165	0.0
10	0.0	0.0	0.0	23.47105447720105	0.0
11	0.0	0.0	0.0	25.537193349161658	0.0
12	0.0	0.0	0.0	27.021581490601818	0.0
13	0.0	0.0	0.0	27.633389495396273	0.0
14	0.0	0.0	0.0	28.088109176844316	0.0
15	0.0	0.0	0.0	29.50962522163793	0.0
16	0.0	0.0	0.0	31.310028375169935	0.0
17	0.0	0.0	0.0	33.25373211155569	0.0
18	0.0	0.0	0.0	34.350408990342146	0.0
19	0.0	0.0	0.0	35.44524750372958	0.0
20	0.0	0.0	0.0	36.6564545468751	0.0
21	0.0	0.0	0.0	37.8868725084404	0.0
22	1.8383653990218057E-4	0.0	0.0	39.094218984247966	0.0
23	2.757548098532709E-4	0.0	0.0	40.0661627707108	0.0
24	4.5959134975545146E-4	0.0	0.0	40.844802435466484	0.0
25	4.5959134975545146E-4	0.0	0.0	41.608919013569896	0.0
26	4.5959134975545146E-4	0.0	0.0	42.3302016778761	0.0
27	7.353461596087223E-4	0.0	0.0	43.04955405851333	0.0
28	0.0010111009694619931	0.0	0.0	43.77175590551905	0.0
29	0.0010111009694619931	0.0	0.0	44.64323302292534	0.0
30	0.0010111009694619931	0.0	0.0	45.42564133674902	0.0
31	0.0010111009694619931	0.0	0.0	46.064932904258846	0.0
32	0.0010111009694619931	0.0	0.0	46.689241793766655	0.0
33	0.0010111009694619931	0.0	0.0	47.337541351731694	0.0
34	0.0010111009694619931	0.0	0.0	48.07729958829807	0.0
35	0.0010111009694619931	0.0	0.0	48.733320280939	0.0
36	0.0010111009694619931	0.0	0.0	49.31130236239146	0.0
37	0.0010111009694619931	0.0	0.0	49.894615703501074	0.0
38	0.0011030192394130835	0.0	0.0	50.47241394841363	0.0
39	0.0011030192394130835	0.0	0.0	51.04938492889662	0.0
40	0.001286855779315264	0.0	0.0	51.650622332646705	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	20	6.311306E-4	46.0	21
TACCCGC	25	3.417035E-5	46.0	30
ACTGCGG	105	0.0	46.0	1
ACGCCCG	20	6.311306E-4	46.0	14
GATCGTC	20	6.311306E-4	46.0	9
GCGCGTT	20	6.311306E-4	46.0	8
CCGTGCT	20	6.311306E-4	46.0	43
GCGCCCT	20	6.311306E-4	46.0	8
CGATTCG	20	6.311306E-4	46.0	9
TGCGGAA	20	6.311306E-4	46.0	3
GTAATAG	20	6.311306E-4	46.0	12
ATATCGG	40	5.6115823E-9	46.0	1
CTAACGG	130	0.0	46.0	1
CACTCCG	30	1.86131E-6	46.0	17
ACGGCCA	30	1.86131E-6	46.0	6
TGCGATT	20	6.311306E-4	46.0	18
TTGCGCT	25	3.417035E-5	46.0	2
GAACGAT	20	6.311306E-4	46.0	8
CGTAAAC	20	6.311306E-4	46.0	23
TTACGGC	50	1.6370905E-11	46.0	2
>>END_MODULE