FastQCFastQC Report
Thu 26 May 2016
SRR1043237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043237_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1068091
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501934.6993186910104106No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC72250.6764404905574525TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT66040.6182993771129989TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC49870.4669077822020783TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG29500.2761936951065031TruSeq Adapter, Index 22 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC27100.2537236995724147TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC22560.21121795802043083No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG20310.190152337207223TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG18510.17329984055665668No Hit
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG15410.14427609632512584TruSeq Adapter, Index 22 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC14740.1380032225718595TruSeq Adapter, Index 22 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC13420.1256447250281109No Hit
TGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT13330.12480210019558259TruSeq Adapter, Index 20 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG13140.12302322554913392TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCC302.165356E-645.0000048
ACCACGG302.165356E-645.0000041
CCAACGG302.165356E-645.0000041
TTACCGG302.165356E-645.0000041
GTACGAT302.165356E-645.00000432
AGCGTAA253.8906197E-545.021
AATCCGG207.033064E-445.01
CGTATCG253.8906197E-545.07
TTTACGG850.045.01
CGCGGGT351.211829E-745.03
CATGCGG850.045.01
CCGGCAC207.033064E-445.026
TTGTCGA207.033064E-445.013
TCGGGTT750.045.03
CGACATA207.033064E-445.011
ATCACGT453.8562575E-1045.015
CCCACGT351.211829E-745.036
CCGTCAC207.033064E-445.017
CCACTAG207.033064E-445.09
ACGGCCA453.8562575E-1045.06
Produced by FastQC (version 0.11.5)