##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043237_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1068091
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.252981253469976	33.0	31.0	34.0	31.0	34.0
2	32.539690906486435	34.0	31.0	34.0	31.0	34.0
3	32.614388661640255	34.0	31.0	34.0	31.0	34.0
4	36.16619838571807	37.0	35.0	37.0	35.0	37.0
5	36.1221328519761	37.0	35.0	37.0	35.0	37.0
6	36.3465715936189	37.0	37.0	37.0	35.0	37.0
7	36.29448708022069	37.0	37.0	37.0	35.0	37.0
8	36.19720136205623	37.0	37.0	37.0	35.0	37.0
9	37.751014660735834	39.0	38.0	39.0	35.0	39.0
10	37.538880114147574	39.0	37.0	39.0	35.0	39.0
11	37.35282855112533	39.0	37.0	39.0	35.0	39.0
12	37.37380054695714	39.0	37.0	39.0	34.0	39.0
13	37.39113333976225	39.0	37.0	39.0	35.0	39.0
14	38.79748542024977	40.0	38.0	41.0	35.0	41.0
15	38.898892510095116	40.0	38.0	41.0	35.0	41.0
16	38.93844719223362	41.0	38.0	41.0	35.0	41.0
17	38.905094228862524	41.0	38.0	41.0	35.0	41.0
18	38.689286774254256	40.0	38.0	41.0	35.0	41.0
19	38.45772410777733	40.0	37.0	41.0	35.0	41.0
20	38.20138452622483	40.0	35.0	41.0	35.0	41.0
21	38.13593598298272	40.0	35.0	41.0	35.0	41.0
22	38.10408663681278	40.0	35.0	41.0	34.0	41.0
23	38.073493737893116	40.0	35.0	41.0	34.0	41.0
24	38.00301566065064	40.0	35.0	41.0	34.0	41.0
25	37.98457528431566	40.0	35.0	41.0	34.0	41.0
26	37.96362669472919	40.0	35.0	41.0	34.0	41.0
27	37.96690169657829	40.0	35.0	41.0	34.0	41.0
28	37.91454286198461	40.0	35.0	41.0	34.0	41.0
29	37.86177394997243	40.0	35.0	41.0	34.0	41.0
30	37.712999173291415	40.0	35.0	41.0	34.0	41.0
31	37.530729123267584	40.0	35.0	41.0	33.0	41.0
32	37.3141876488052	40.0	35.0	41.0	33.0	41.0
33	37.018340197604886	40.0	35.0	41.0	33.0	41.0
34	36.68017238231574	39.0	35.0	41.0	31.0	41.0
35	36.58266383669556	40.0	35.0	41.0	31.0	41.0
36	36.45596770312642	39.0	35.0	41.0	31.0	41.0
37	36.32190515602135	39.0	35.0	41.0	30.0	41.0
38	36.30001189037264	39.0	35.0	41.0	30.0	41.0
39	36.27200865843828	39.0	35.0	41.0	30.0	41.0
40	36.213146632637105	39.0	35.0	41.0	30.0	41.0
41	36.168281541554045	39.0	35.0	41.0	30.0	41.0
42	36.09890355784292	39.0	35.0	41.0	30.0	41.0
43	36.00039041617241	39.0	35.0	41.0	30.0	41.0
44	35.901722793282595	39.0	35.0	41.0	29.0	41.0
45	35.84262764127776	39.0	35.0	41.0	29.0	41.0
46	35.75559011357647	39.0	35.0	40.0	29.0	41.0
47	35.6204649229326	38.0	35.0	40.0	28.0	41.0
48	35.62209212510919	38.0	35.0	40.0	28.0	41.0
49	35.57082027654947	38.0	35.0	40.0	27.0	41.0
50	35.47486309687096	38.0	35.0	40.0	27.0	41.0
51	35.04596986586349	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	5.0
16	29.0
17	52.0
18	99.0
19	235.0
20	486.0
21	914.0
22	1476.0
23	2471.0
24	4502.0
25	8980.0
26	14317.0
27	17120.0
28	16059.0
29	15249.0
30	16138.0
31	18605.0
32	22508.0
33	29825.0
34	54273.0
35	95553.0
36	91643.0
37	84301.0
38	152606.0
39	420593.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.81972697082927	19.8062711885036	26.36161151062971	26.012390330037423
2	32.98941756835326	20.143695621440493	27.24702295965419	19.61986385055206
3	21.910399020308194	22.124238477807605	36.1813740589519	19.783988442932294
4	23.480396333271226	24.67580009568473	33.27141601230606	18.572387558737972
5	20.684660763923674	30.369509714059944	29.91702017899224	19.028809343024143
6	75.4849539973654	1.4906969537239805	19.318204160506923	3.7061448884037036
7	76.6333580191201	1.2468975021791215	15.801088109533737	6.318656369167047
8	65.29059789849366	3.3167585907942305	23.234443507154353	8.158200003557749
9	32.66079388366722	25.759509255297537	25.729081136345123	15.850615724690126
10	24.082311338640622	19.758709697956448	43.46633386106615	12.692645102336787
11	22.00814350088148	17.62761787151095	39.89613244564368	20.468106181963897
12	17.37829454606396	21.772021297810767	43.103349808209224	17.746334347916047
13	13.693402528436247	24.891886552737546	41.90401379657725	19.510697122248946
14	12.397351911026307	23.975297984909524	43.599936709512576	20.027413394551587
15	13.451007451612268	20.393112571868876	46.37161065864238	19.784269317876475
16	13.307386730156887	24.81389694323798	38.923743388906004	22.95497293769913
17	14.728239447762409	22.148487347988137	40.68642091357385	22.436852290675606
18	16.299360260502148	27.398227304602322	39.51891739561517	16.78349503928036
19	16.614314697904952	23.551270444185	36.58826822808169	23.246146629828356
20	14.475826497929484	29.13356633470369	35.88477011790194	20.505837049464887
21	15.260403841994735	23.681409168319927	36.86043604898834	24.197750940697
22	12.958633674471557	26.11715668421511	33.23209352012142	27.692116121191923
23	12.496032641413512	22.83616283631264	43.11467843095766	21.553126091316187
24	16.764208293113604	22.265518574728183	38.84060440542987	22.12966872672834
25	13.084184774518276	27.085426241771533	37.44353243309793	22.38685655061226
26	14.537244485722658	24.463458637887594	36.85547392497456	24.14382295141519
27	18.680149912320203	25.723463637461602	37.2004819814042	18.39590446881399
28	14.338946775134328	25.579562041062044	34.57542475313433	25.506066430669293
29	17.627524246529553	28.323991120606767	31.896065035657074	22.152419597206606
30	20.999802451289263	27.548589024717927	30.122901513073323	21.32870701091948
31	19.04182321543764	29.138434833736078	30.602823167688896	21.21691878313739
32	19.610407727431465	28.879842635131276	29.41135165449386	22.098397982943403
33	17.513395394212665	29.383638660001814	30.352376342465202	22.750589603320318
34	17.223532451822926	26.926170148423683	34.439949405060055	21.410347994693335
35	23.010024426757646	25.460190189787202	28.668905552055023	22.860879831400133
36	21.67502581708862	24.69742746638629	30.990056090726352	22.637490625798737
37	19.22607717881716	23.59649131019735	36.0943964512387	21.083035059746784
38	17.78312896560312	21.966948509068985	31.256700037730866	28.99322248759703
39	22.27722169740219	20.364088827637346	32.38169781413756	24.976991660822907
40	15.747160120251927	20.271774595984798	36.99563052211843	26.98543476164484
41	17.667127613658387	25.65942415018945	29.62434848716074	27.049099748991424
42	19.234128927216876	21.684950065116173	33.7871024098134	25.29381859785355
43	23.543967695636418	23.591435561202182	31.560232227403844	21.304364515757552
44	18.513497445442383	25.07333176667531	32.89672883677514	23.51644195110716
45	20.464735682633783	22.446402038777595	33.13041679032966	23.95844548825896
46	22.96012231167569	20.739150503093835	32.41989680654551	23.880830378684962
47	17.168949087671372	19.75421569884963	36.17238606073827	26.904449152740728
48	18.852607128044333	17.7551350961669	31.852997544216738	31.53926023157203
49	21.092865682792947	17.791929713853968	36.447362631086676	24.66784197226641
50	19.314084661325673	16.956701254855624	37.04375376255394	26.68546032126476
51	17.230741575390113	16.9593227543346	35.101690773538955	30.708244896736325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	306.0
1	1413.5
2	2521.0
3	30099.5
4	57678.0
5	32558.5
6	7439.0
7	7078.5
8	6718.0
9	6983.0
10	7248.0
11	7290.5
12	7333.0
13	7083.5
14	6834.0
15	6442.5
16	6051.0
17	5529.0
18	5007.0
19	4665.0
20	4323.0
21	4213.5
22	4104.0
23	3910.5
24	3717.0
25	4045.5
26	5295.0
27	6216.0
28	6665.0
29	7114.0
30	8436.5
31	9759.0
32	11750.0
33	13741.0
34	16511.0
35	19281.0
36	21465.0
37	23649.0
38	26971.0
39	30293.0
40	35436.5
41	40580.0
42	49921.5
43	59263.0
44	69413.0
45	79563.0
46	100236.5
47	120910.0
48	143607.0
49	166304.0
50	160630.0
51	154956.0
52	121520.5
53	88085.0
54	67192.5
55	46300.0
56	35757.0
57	25214.0
58	21022.5
59	16831.0
60	13889.5
61	10948.0
62	9291.5
63	7635.0
64	6324.5
65	5014.0
66	4246.5
67	3479.0
68	2927.5
69	2376.0
70	2255.5
71	2135.0
72	2071.0
73	2007.0
74	1658.5
75	989.0
76	668.0
77	551.0
78	434.0
79	327.0
80	220.0
81	137.5
82	55.0
83	64.5
84	74.0
85	46.0
86	18.0
87	11.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1068091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.04291177290992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.10527018044228	22.073146288003926
2	12.47078060697916	7.742586842435402
3	5.07607291913567	4.727282513447577
4	2.6913718568987557	3.3419207640720323
5	1.5606233600444508	2.422314663830106
6	1.0337846287713868	1.9255011013884358
7	0.7341314047041254	1.5952703498166816
8	0.5630439728538464	1.3982819498856516
9	0.40690516319291137	1.1368368972845155
>10	3.791848456240685	25.74818240106596
>50	0.38870772438872697	8.012912214220913
>100	0.1628489770090214	9.304693066037862
>500	0.009740499559318598	2.076266319412471
>1k	0.003957077945973181	2.445256442623034
>5k	6.087812224574124E-4	1.306725842222503
>10k+	3.043906112287062E-4	4.742822344252954
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50193	4.6993186910104106	No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	7225	0.6764404905574525	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	6604	0.6182993771129989	TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	4987	0.4669077822020783	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	2950	0.2761936951065031	TruSeq Adapter, Index 22 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2710	0.2537236995724147	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC	2256	0.21121795802043083	No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	2031	0.190152337207223	TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	1851	0.17329984055665668	No Hit
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	1541	0.14427609632512584	TruSeq Adapter, Index 22 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	1474	0.1380032225718595	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	1342	0.1256447250281109	No Hit
TGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT	1333	0.12480210019558259	TruSeq Adapter, Index 20 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	1314	0.12302322554913392	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6354327487077412	0.0
2	0.0	0.0	0.0	2.2168523094006036	0.0
3	0.0	0.0	0.0	3.6923820161390744	0.0
4	0.0	0.0	0.0	4.617771332217948	0.0
5	0.0	0.0	0.0	9.238913163766009	0.0
6	0.0	0.0	0.0	10.264668459897143	0.0
7	0.0	0.0	0.0	13.908365485712359	0.0
8	0.0	0.0	0.0	18.457509706569947	0.0
9	0.0	0.0	0.0	21.794584918326247	0.0
10	0.0	0.0	0.0	24.004228104159665	0.0
11	0.0	0.0	0.0	26.13054505655417	0.0
12	0.0	0.0	0.0	27.632102508119626	0.0
13	0.0	0.0	0.0	28.268752381585465	0.0
14	0.0	0.0	0.0	28.727795665350612	0.0
15	0.0	0.0	0.0	30.213436870079423	0.0
16	0.0	0.0	0.0	32.07367162535776	0.0
17	0.0	0.0	0.0	34.081740226254134	0.0
18	0.0	0.0	0.0	35.21001487700954	0.0
19	0.0	0.0	0.0	36.36272564790828	0.0
20	0.0	0.0	0.0	37.58368903024181	0.0
21	0.0	0.0	0.0	38.83667215621141	0.0
22	1.872499627840699E-4	0.0	0.0	40.09237040664138	0.0
23	1.872499627840699E-4	0.0	0.0	41.07580721118332	0.0
24	4.6812490696017476E-4	0.0	0.0	41.862444304839194	0.0
25	4.6812490696017476E-4	0.0	0.0	42.621462028984425	0.0
26	4.6812490696017476E-4	0.0	0.0	43.34031463611247	0.0
27	4.6812490696017476E-4	0.0	0.0	44.03978687209236	0.0
28	8.426248325283146E-4	0.0	0.0	44.77502385096401	0.0
29	8.426248325283146E-4	0.0	0.0	45.65893730028621	0.0
30	8.426248325283146E-4	0.0	0.0	46.43873977030047	0.0
31	8.426248325283146E-4	0.0	0.0	47.07361076911986	0.0
32	8.426248325283146E-4	0.0	0.0	47.706890143255585	0.0
33	8.426248325283146E-4	0.0	0.0	48.38342425879443	0.0
34	8.426248325283146E-4	0.0	0.0	49.10227686592247	0.0
35	8.426248325283146E-4	0.0	0.0	49.7684186085268	0.0
36	8.426248325283146E-4	0.0	0.0	50.364903364975454	0.0
37	8.426248325283146E-4	0.0	0.0	50.95492799770806	0.0
38	8.426248325283146E-4	0.0	0.0	51.52997263341794	0.0
39	8.426248325283146E-4	0.0	0.0	52.10267664459302	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCC	30	2.165356E-6	45.000004	8
ACCACGG	30	2.165356E-6	45.000004	1
CCAACGG	30	2.165356E-6	45.000004	1
TTACCGG	30	2.165356E-6	45.000004	1
GTACGAT	30	2.165356E-6	45.000004	32
AGCGTAA	25	3.8906197E-5	45.0	21
AATCCGG	20	7.033064E-4	45.0	1
CGTATCG	25	3.8906197E-5	45.0	7
TTTACGG	85	0.0	45.0	1
CGCGGGT	35	1.211829E-7	45.0	3
CATGCGG	85	0.0	45.0	1
CCGGCAC	20	7.033064E-4	45.0	26
TTGTCGA	20	7.033064E-4	45.0	13
TCGGGTT	75	0.0	45.0	3
CGACATA	20	7.033064E-4	45.0	11
ATCACGT	45	3.8562575E-10	45.0	15
CCCACGT	35	1.211829E-7	45.0	36
CCGTCAC	20	7.033064E-4	45.0	17
CCACTAG	20	7.033064E-4	45.0	9
ACGGCCA	45	3.8562575E-10	45.0	6
>>END_MODULE