SRR1043236_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043236_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	401958
Sequences flagged as poor quality	0
Sequence length	52
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30251	7.525910667283646	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	3639	0.9053184661083994	Illumina PCR Primer Index 2 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	3228	0.8030689773558431	Illumina PCR Primer Index 2 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2828	0.7035560929251314	Illumina PCR Primer Index 2 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1371	0.3410804113862642	Illumina PCR Primer Index 2 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	1333	0.3316266873653466	Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	1131	0.2813726807278373	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1088	0.2706750456515357	Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	985	0.24505047791062748	Illumina PCR Primer Index 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	789	0.19628916453957876	Illumina PCR Primer Index 2 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	758	0.1885769159961986	Illumina PCR Primer Index 2 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	687	0.1709133790097473	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	644	0.1602157439334458	Illumina PCR Primer Index 2 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	593	0.14752785116853004	Illumina PCR Primer Index 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	563	0.14006438483622669	Illumina PCR Primer Index 2 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	553	0.13757656272545887	Illumina PCR Primer Index 2 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTTG	494	0.12289841227192892	Illumina PCR Primer Index 2 (96% over 27bp)
ATTGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	462	0.11493738151747199	Illumina PCR Primer Index 2 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	458	0.11394225267316485	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	454	0.11294712382885774	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	439	0.10921539066270605	Illumina PCR Primer Index 2 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	421	0.10473731086332402	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	419	0.10423974644117047	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC	417	0.10374218201901692	No Hit
ATAGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	414	0.10299583538578658	Illumina PCR Primer Index 2 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTCAT	25	3.4131634E-5	46.0	20
TCACGAC	90	0.0	46.0	24
CTTGTGG	20	6.3065335E-4	46.0	1
CGGGTAG	20	6.3065335E-4	46.0	5
CGGGTAA	50	1.6370905E-11	46.0	5
GTCGCGG	25	3.4131634E-5	46.0	1
GTACCGG	20	6.3065335E-4	46.0	1
TCGCAGG	20	6.3065335E-4	46.0	1
ACAAAGG	30	1.8583614E-6	46.0	1
GAAGCAC	25	3.4131634E-5	46.0	8
GGAGCAT	20	6.3065335E-4	46.0	8
ACTGCGG	45	3.092282E-10	46.0	1
GTACATG	30	1.8583614E-6	46.0	40
GTATTGA	40	5.5952114E-9	46.0	25
CTGTAGG	40	5.5952114E-9	46.0	1
TACCATT	20	6.3065335E-4	46.0	4
GGTAAGT	20	6.3065335E-4	46.0	7
TGCTTGG	45	3.092282E-10	46.0	1
GGTAACA	25	3.4131634E-5	46.0	7
CGACGTT	30	1.8583614E-6	46.0	26

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content