SRR1043235_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043235_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	395741
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27628	6.981333751114997	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3480	0.8793630177312939	Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	3191	0.8063354567760228	Illumina PCR Primer Index 2 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2808	0.7095549867211131	Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	1346	0.34012144306503495	Illumina PCR Primer Index 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1302	0.3290030600822255	Illumina PCR Primer Index 2 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	1076	0.2718950020341587	Illumina PCR Primer Index 2 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC	1076	0.2718950020341587	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	930	0.23500218577301818	Illumina PCR Primer Index 2 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	801	0.20240510839159956	Illumina PCR Primer Index 2 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	786	0.1986147505565509	Illumina PCR Primer Index 2 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	663	0.1675338163091517	Illumina PCR Primer Index 2 (95% over 23bp)
CGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	622	0.15717350489335197	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	616	0.15565736175933248	Illumina PCR Primer Index 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	607	0.15338314705830328	Illumina PCR Primer Index 2 (96% over 26bp)
TGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	552	0.13948516832979144	Illumina PCR Primer Index 2 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	517	0.1306410000480112	Illumina PCR Primer Index 2 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	466	0.1177537834088457	Illumina PCR Primer Index 2 (95% over 23bp)
ATAGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	447	0.11295266348445068	Illumina PCR Primer Index 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	437	0.1104257582610849	Illumina PCR Primer Index 2 (95% over 22bp)
ATTGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	413	0.10436118572500701	Illumina PCR Primer Index 2 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTC	406	0.10259235206865096	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	400	0.1010762089346315	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	397	0.10031813736762175	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTAGG	65	0.0	45.000004	1
TGGAGCG	35	1.2092278E-7	45.000004	35
TCGCAGG	30	2.1618798E-6	45.000004	1
CAAACGG	35	1.2092278E-7	45.000004	1
GGAGCGT	35	1.2092278E-7	45.000004	36
GTTCTGG	35	1.2092278E-7	45.000004	1
AATCAGG	30	2.1618798E-6	45.000004	1
CGACGGT	30	2.1618798E-6	45.000004	27
CATGCGG	35	1.2092278E-7	45.000004	1
TGGGTAC	30	2.1618798E-6	45.000004	5
GCTATGG	65	0.0	45.000004	1
ATTACGG	35	1.2092278E-7	45.000004	1
TAGCGCT	30	2.1618798E-6	45.000004	2
ACCGAGG	30	2.1618798E-6	45.000004	1
CCGTGGG	30	2.1618798E-6	45.000004	2
GGTCACA	30	2.1618798E-6	45.000004	8
CACGAGG	30	2.1618798E-6	45.000004	1
TGTGAGG	70	0.0	45.000004	1
ATCAAAG	30	2.1618798E-6	45.000004	10
ACGATGG	30	2.1618798E-6	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content