FastQCFastQC Report
Thu 26 May 2016
SRR1043234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043234_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences497532
Sequences flagged as poor quality0
Sequence length52
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8915817.92005338350096No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT26280.5282072308916813TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT19850.3989693125266315TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT14990.30128715338912876TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG13540.27214329932547054No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC11090.2229002355627377TruSeq Adapter, Index 15 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG9470.19033951585023676No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTG9030.1814958635826439No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT7850.15777879613773585TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6670.1340617286928278No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6570.13205180772292033No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT6110.122806171261346No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC5530.1111486296358827No Hit
ACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC5420.1089377165689845TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAC750.046.00000413
GCCTATG750.046.0000048
TGCAAGG750.046.0000041
GTTGAGG1700.046.01
GTTTGGC206.307987E-446.021
ATGGGTG206.307987E-446.04
CAGGTAT206.307987E-446.026
AACGTCG206.307987E-446.042
TGGAGTT206.307987E-446.022
AGCCGTC206.307987E-446.034
ATCTCGG253.4143428E-546.01
GCCTTGC206.307987E-446.016
CCGGGTC206.307987E-446.012
CAAACTA206.307987E-446.042
GCCTTAG206.307987E-446.016
ACACGGT206.307987E-446.030
ATAGGTG1350.046.023
CAACGCA501.6370905E-1146.016
CGACGGT301.85926E-646.027
GAGTCTA253.4143428E-546.027
Produced by FastQC (version 0.11.5)