##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043234_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	497532
Sequences flagged as poor quality	0
Sequence length	52
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70840066568582	34.0	31.0	34.0	31.0	34.0
2	32.97947870689725	34.0	31.0	34.0	31.0	34.0
3	32.894089224411694	34.0	31.0	34.0	31.0	34.0
4	36.41741837710941	37.0	37.0	37.0	35.0	37.0
5	36.34584710129198	37.0	37.0	37.0	35.0	37.0
6	36.50120796250292	37.0	37.0	37.0	35.0	37.0
7	36.46961401477694	37.0	37.0	37.0	35.0	37.0
8	36.48487534470144	37.0	37.0	37.0	35.0	37.0
9	38.17209345328542	39.0	39.0	39.0	37.0	39.0
10	38.01971531479382	39.0	39.0	39.0	35.0	39.0
11	37.56782478312953	39.0	37.0	39.0	35.0	39.0
12	37.47354341027311	39.0	37.0	39.0	35.0	39.0
13	37.508407499417125	39.0	37.0	39.0	35.0	39.0
14	39.02144384682794	41.0	38.0	41.0	35.0	41.0
15	39.081845991815605	41.0	38.0	41.0	35.0	41.0
16	39.08700143910342	41.0	38.0	41.0	35.0	41.0
17	39.089887685616205	41.0	38.0	41.0	35.0	41.0
18	38.38221260140051	39.0	38.0	41.0	35.0	41.0
19	37.6109134688824	37.0	37.0	41.0	35.0	41.0
20	36.843350779447356	35.0	35.0	41.0	35.0	41.0
21	36.77973477082881	35.0	35.0	41.0	35.0	41.0
22	36.738354517900355	35.0	35.0	40.0	35.0	41.0
23	36.732288576413175	35.0	35.0	40.0	35.0	41.0
24	36.63229299823931	35.0	35.0	40.0	35.0	41.0
25	36.56061720653144	35.0	35.0	40.0	34.0	41.0
26	36.490499103575246	35.0	35.0	40.0	34.0	41.0
27	36.48194688984829	35.0	35.0	40.0	34.0	41.0
28	36.37266949663539	35.0	35.0	40.0	34.0	41.0
29	36.247451420210155	35.0	35.0	40.0	33.0	41.0
30	36.00825273550244	35.0	35.0	40.0	33.0	41.0
31	35.573545018209884	35.0	35.0	40.0	32.0	41.0
32	34.97995907800905	35.0	35.0	40.0	30.0	41.0
33	34.22894808776119	35.0	35.0	40.0	21.0	41.0
34	33.55421359832131	35.0	35.0	40.0	16.0	41.0
35	33.04646736290329	35.0	35.0	40.0	15.0	41.0
36	32.8744522965357	35.0	34.0	40.0	11.0	41.0
37	32.81752128506307	35.0	34.0	40.0	10.0	41.0
38	32.78226124148799	35.0	34.0	40.0	10.0	41.0
39	32.71877587773249	35.0	33.0	40.0	10.0	41.0
40	32.68989532331589	35.0	34.0	40.0	10.0	41.0
41	32.55755207705233	35.0	34.0	40.0	10.0	41.0
42	32.39765482421231	35.0	33.0	40.0	10.0	41.0
43	32.19143492277884	35.0	33.0	40.0	10.0	41.0
44	31.96981299695296	35.0	33.0	40.0	8.0	41.0
45	31.825790099933272	35.0	33.0	40.0	8.0	41.0
46	31.740340319818625	35.0	33.0	40.0	8.0	41.0
47	31.65067372550911	35.0	33.0	40.0	8.0	41.0
48	31.569824654494585	35.0	32.0	40.0	7.0	41.0
49	31.488668065571662	35.0	32.0	40.0	7.0	41.0
50	31.366511098783597	35.0	32.0	40.0	7.0	41.0
51	31.24218140742706	35.0	31.0	40.0	7.0	41.0
52	30.96247276557086	35.0	31.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	4.0
15	9.0
16	15.0
17	53.0
18	148.0
19	295.0
20	600.0
21	955.0
22	1659.0
23	3136.0
24	6617.0
25	12980.0
26	18657.0
27	19077.0
28	15011.0
29	11475.0
30	10014.0
31	9876.0
32	11100.0
33	13780.0
34	28235.0
35	69540.0
36	77171.0
37	23511.0
38	40383.0
39	123072.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.513719720540585	13.654599101163342	17.361697338060665	49.46998384023541
2	52.239654936767884	14.750207021859902	18.77627971668154	14.233858324690674
3	15.917770113279145	15.159225939236068	54.95023435678509	13.972769590699693
4	16.64556249648264	17.12191376635071	53.023323122934805	13.209200614231847
5	14.267022020694146	21.13230907760707	50.85723129366553	13.743437608033252
6	53.553942258990375	1.3806147142294365	42.58198467636253	2.4834583504176617
7	54.97093654277514	1.0724938295426223	40.403632329176816	3.552937298505423
8	48.98257800503284	2.1017743582322344	44.13846747545887	4.777180161276059
9	28.502086297966766	14.96828344709486	46.41852182372189	10.111108431216485
10	24.14719053246826	12.880377543554989	54.881696051711245	8.090735872265503
11	22.229725927176545	12.546730662550349	53.15416897807578	12.069374432197327
12	11.953401992233665	23.28654237315389	53.95733339765081	10.802722236961642
13	8.464782164765282	25.106927795599077	54.55628984668323	11.872000192952413
14	7.1394402772083	16.561949784134487	63.726755263983016	12.57185467467419
15	7.497809186142801	12.789931099909152	64.71181753133467	15.00044218261338
16	8.10902615309166	15.756976435686548	53.46630970470241	22.66768770651938
17	10.751268260132012	15.289468818086071	57.75246617303008	16.206796748751838
18	13.266483361874212	18.71759002436024	54.09722389715637	13.918702716609182
19	14.929290980278656	16.156950708698133	53.04201538795495	15.871742923068267
20	10.590876566733396	20.961063810970952	53.8554304044765	14.592629217819155
21	11.961441676113296	16.95328139697547	52.77369093847229	18.311585988438935
22	9.453663281959754	20.10343053311144	50.68437809025349	19.758528094675317
23	8.991783443075018	16.099868953152765	61.69753905276445	13.210808551007775
24	10.988438934581092	16.282168785123368	57.528158992788406	15.201233287507135
25	8.824357026281726	21.660717300595742	53.50490018732464	16.0100254857979
26	9.558782148685914	20.183023403519773	53.7097111341582	16.54848331363611
27	11.441274129101243	17.684892630021785	57.54705224990554	13.326780990971434
28	8.336147222691203	15.895258998416182	56.152770073080724	19.615823705811888
29	10.945627617922064	18.570865793556997	55.62476383428604	14.858742754234905
30	16.739224813680327	17.280898515070387	50.1587837566227	15.821092914626597
31	16.507279933753004	17.868197422477348	51.41277344974795	14.211749194021692
32	16.68274603442593	16.98001334587524	51.0403350940241	15.296905525674731
33	11.888481544905655	16.768167675646993	52.33874404058433	19.00460673886303
34	15.435389080501356	17.099201659390754	49.90754363538426	17.557865624723636
35	19.316948457586648	16.37120828409027	49.14457763520738	15.1672656231157
36	17.349637812241223	20.109862280215143	47.917118898884894	14.62338100865874
37	15.620703793926824	19.939822966160968	49.8522708087118	14.587202431200405
38	15.1433475635738	20.866597525385302	47.8996325864467	16.090422324594197
39	19.68436201088573	17.06905284484214	48.12172885362148	15.124856290650651
40	13.498227249704541	15.874556812426135	50.7971346566653	19.830081281204023
41	15.529051397699043	18.166268702314625	45.42421391990867	20.880465980077663
42	15.946913967342805	17.029256409637973	47.290023556273766	19.733806066745455
43	17.502190813857197	19.21524645650933	46.116229709847815	17.16633301978566
44	13.364165521011714	23.436080493315004	43.79858983944751	19.40116414622577
45	15.9621893667141	19.998512658482266	45.78760763126794	18.251690343535692
46	18.254705224990552	17.77172121592179	45.426625825072556	18.5469477340151
47	14.610718506548324	16.666867658763657	49.723434874540736	18.998978960147287
48	15.819685969947662	14.92507014624185	47.30369101886914	21.951552864941352
49	16.285585650772212	19.005611699347984	47.235755690086265	17.473046959793542
50	14.425202801025863	18.083459958354435	46.72101492969297	20.770322310926733
51	13.805946150197373	15.534880168511775	47.50408013956891	23.15509354172194
52	15.755368498910624	15.77365877973678	49.044885555099974	19.42608716625262
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	617.0
2	1079.0
3	48567.0
4	96055.0
5	52708.5
6	9362.0
7	9177.0
8	8992.0
9	9515.5
10	10039.0
11	10389.5
12	10740.0
13	10355.0
14	9220.0
15	8470.0
16	7623.5
17	6777.0
18	6201.0
19	5625.0
20	5195.0
21	4765.0
22	4551.0
23	4337.0
24	4104.5
25	3872.0
26	3902.5
27	3933.0
28	3627.0
29	3321.0
30	3596.5
31	3872.0
32	4668.5
33	5465.0
34	5738.5
35	6012.0
36	7085.5
37	8159.0
38	8632.0
39	9422.5
40	9740.0
41	14130.5
42	18521.0
43	24911.0
44	31301.0
45	34643.5
46	37986.0
47	41714.5
48	45443.0
49	44848.0
50	44253.0
51	37652.0
52	31051.0
53	24404.5
54	17758.0
55	14876.0
56	11994.0
57	10526.0
58	9058.0
59	7619.0
60	6180.0
61	5134.5
62	4089.0
63	3590.5
64	2467.0
65	1842.0
66	1602.5
67	1363.0
68	1117.0
69	871.0
70	834.5
71	798.0
72	843.0
73	888.0
74	744.0
75	600.0
76	447.5
77	295.0
78	229.5
79	164.0
80	122.5
81	81.0
82	60.5
83	40.0
84	26.5
85	13.0
86	7.0
87	1.0
88	2.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	497532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.747209804596885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85907360113276	27.625153312273653
2	8.372908619806386	5.651246077346477
3	2.7948417281238176	2.8295433050890977
4	1.3795974493122194	1.862302582713047
5	0.812690312813878	1.3713015246346707
6	0.5432160528747267	1.099921566335304
7	0.4119225216644872	0.9730865033295039
8	0.3570464179564411	0.9639456301404644
9	0.26224031683894855	0.7964891092429159
>10	2.6329948008157906	20.176830911804675
>50	0.4785863468113636	10.756092224113493
>100	0.08652745671776524	5.071439787306924
>500	0.004773928646497392	1.140837326573233
>1k	0.0029837054040608704	1.7268632083610718
>5k	0.0	0.0
>10k+	5.96741080812174E-4	17.954946930735446
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89158	17.92005338350096	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	2628	0.5282072308916813	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT	1985	0.3989693125266315	TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	1499	0.30128715338912876	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG	1354	0.27214329932547054	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1109	0.2229002355627377	TruSeq Adapter, Index 15 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG	947	0.19033951585023676	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTG	903	0.1814958635826439	No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	785	0.15777879613773585	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	667	0.1340617286928278	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	657	0.13205180772292033	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT	611	0.122806171261346	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC	553	0.1111486296358827	No Hit
ACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	542	0.1089377165689845	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.411229830443067	0.0
2	0.0	0.0	0.0	1.5114605693704124	0.0
3	0.0	0.0	0.0	2.4621531881366425	0.0
4	0.0	0.0	0.0	3.1728612430959213	0.0
5	0.0	0.0	0.0	5.919820232668451	0.0
6	0.0	0.0	0.0	6.437173890322632	0.0
7	0.0	0.0	0.0	8.091740832750457	0.0
8	0.0	0.0	0.0	10.177837807417413	0.0
9	0.0	0.0	0.0	11.500365805616523	0.0
10	0.0	0.0	0.0	12.610445157296414	0.0
11	0.0	0.0	0.0	13.80775507907029	0.0
12	0.0	0.0	0.0	14.648103036588601	0.0
13	0.0	0.0	0.0	14.989186625181897	0.0
14	0.0	0.0	0.0	15.265550758544174	0.0
15	0.0	0.0	0.0	16.035149497921743	0.0
16	0.0	0.0	0.0	16.93680004502223	0.0
17	0.0	0.0	0.0	18.011906771825732	0.0
18	0.0	0.0	0.0	18.517602887854448	0.0
19	0.0	0.0	0.0	19.06992917038502	0.0
20	0.0	0.0	0.0	19.63773184438388	0.0
21	0.0	0.0	0.0	20.17940554577394	0.0
22	0.0	0.0	0.0	20.742786393639	0.0
23	2.0099209699074632E-4	0.0	0.0	21.176326346848043	0.0
24	0.001205952581944478	0.0	0.0	21.551779584026757	0.0
25	0.001205952581944478	0.0	0.0	21.912359406028155	0.0
26	0.001205952581944478	0.0	0.0	22.2502271210696	0.0
27	0.001205952581944478	0.0	0.0	22.588295828208036	0.0
28	0.001205952581944478	0.0	0.0	22.921942709212672	0.0
29	0.001205952581944478	0.0	0.0	23.34663901015412	0.0
30	0.001205952581944478	0.0	0.0	23.700586092954826	0.0
31	0.001205952581944478	0.0	0.0	24.004486143604833	0.0
32	0.001205952581944478	0.0	0.0	24.2963266684354	0.0
33	0.001205952581944478	0.0	0.0	24.580328501483322	0.0
34	0.001205952581944478	0.0	0.0	24.969449201257408	0.0
35	0.001205952581944478	0.0	0.0	25.26108873399098	0.0
36	0.001205952581944478	0.0	0.0	25.54870842478474	0.0
37	0.0014069446789352243	0.0	0.0	25.82406759766206	0.0
38	0.0016079367759259705	0.0	0.0	26.11691308297758	0.0
39	0.002009920969907463	0.0	0.0	26.39548812940675	0.0
40	0.0022109130668982097	0.0	0.0	26.68873559891625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCTAC	75	0.0	46.000004	13
GCCTATG	75	0.0	46.000004	8
TGCAAGG	75	0.0	46.000004	1
GTTGAGG	170	0.0	46.0	1
GTTTGGC	20	6.307987E-4	46.0	21
ATGGGTG	20	6.307987E-4	46.0	4
CAGGTAT	20	6.307987E-4	46.0	26
AACGTCG	20	6.307987E-4	46.0	42
TGGAGTT	20	6.307987E-4	46.0	22
AGCCGTC	20	6.307987E-4	46.0	34
ATCTCGG	25	3.4143428E-5	46.0	1
GCCTTGC	20	6.307987E-4	46.0	16
CCGGGTC	20	6.307987E-4	46.0	12
CAAACTA	20	6.307987E-4	46.0	42
GCCTTAG	20	6.307987E-4	46.0	16
ACACGGT	20	6.307987E-4	46.0	30
ATAGGTG	135	0.0	46.0	23
CAACGCA	50	1.6370905E-11	46.0	16
CGACGGT	30	1.85926E-6	46.0	27
GAGTCTA	25	3.4143428E-5	46.0	27
>>END_MODULE