FastQCFastQC Report
Thu 26 May 2016
SRR1043233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043233_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences485320
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8472017.45652353086623No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC26460.5452072859144482TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT19760.40715404269348066TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC15140.3119591197560373TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC13190.2717794444902332No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG10730.2210912387702959No Hit
CGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT9900.20398912058023572No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT8750.18029341465424875No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC7990.16463364378142256TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC6330.13042940740130224No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6080.1252781669826094No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5930.12218742273139371No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC5480.11291518997774665No Hit
GCCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG5110.10529135415808127No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAA207.0292526E-445.00000426
TCACGAC453.8380676E-1045.00000424
CTTAAGG351.2099918E-745.0000041
GTCATGG351.2099918E-745.0000041
GTCATAA207.0292526E-445.00000444
AGGTATG207.0292526E-445.00000414
TGTCAGG302.1629003E-645.0000041
CCTTAGC207.0292526E-445.00000442
CTCACGA453.8380676E-1045.00000423
TCGCAGG351.2099918E-745.0000041
GCCCACA406.8012014E-945.00000412
ACCGGGT207.0292526E-445.0000043
AGATCGT207.0292526E-445.00000442
ACCGGCC302.1629003E-645.00000420
TGCCCAC406.8012014E-945.00000411
GATAATC207.0292526E-445.0000048
TTAATCG302.1629003E-645.00000419
ATAGGTG900.045.00000423
TTTACGG600.045.0000041
CGACGGT453.8380676E-1045.00000427