##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043233_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	485320
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53181612132202	33.0	31.0	34.0	31.0	34.0
2	32.883141020357705	34.0	31.0	34.0	31.0	34.0
3	32.790340393966865	34.0	31.0	34.0	31.0	34.0
4	36.377072859144484	37.0	37.0	37.0	35.0	37.0
5	36.35199249979395	37.0	37.0	37.0	35.0	37.0
6	36.50134138300503	37.0	37.0	37.0	35.0	37.0
7	36.49179922525344	37.0	37.0	37.0	35.0	37.0
8	36.444411934393806	37.0	37.0	37.0	35.0	37.0
9	38.133579906041376	39.0	39.0	39.0	37.0	39.0
10	37.97380697271903	39.0	39.0	39.0	35.0	39.0
11	37.5558353251463	39.0	37.0	39.0	35.0	39.0
12	37.44433363553944	39.0	37.0	39.0	35.0	39.0
13	37.484688452979476	39.0	37.0	39.0	35.0	39.0
14	39.004553696530124	41.0	38.0	41.0	35.0	41.0
15	39.07815668012857	41.0	38.0	41.0	35.0	41.0
16	39.09226077639496	41.0	38.0	41.0	35.0	41.0
17	39.086196736174074	41.0	38.0	41.0	35.0	41.0
18	38.4090785461139	39.0	38.0	41.0	35.0	41.0
19	37.67344020440122	37.0	37.0	41.0	35.0	41.0
20	36.92076568037584	35.0	35.0	41.0	35.0	41.0
21	36.86394749855765	35.0	35.0	41.0	35.0	41.0
22	36.826903898458745	35.0	35.0	41.0	35.0	41.0
23	36.79409049699168	35.0	35.0	40.0	35.0	41.0
24	36.70159688452979	35.0	35.0	40.0	35.0	41.0
25	36.67315379543394	35.0	35.0	40.0	35.0	41.0
26	36.58906288634303	35.0	35.0	40.0	34.0	41.0
27	36.58586705678727	35.0	35.0	40.0	34.0	41.0
28	36.48245487513393	35.0	35.0	40.0	34.0	41.0
29	36.377044012198134	35.0	35.0	40.0	34.0	41.0
30	36.089233907524935	35.0	35.0	40.0	33.0	41.0
31	35.64656103189648	35.0	35.0	40.0	33.0	41.0
32	35.065480507706255	35.0	35.0	40.0	30.0	41.0
33	34.276413500370886	35.0	35.0	40.0	21.0	41.0
34	33.57980095607022	35.0	35.0	40.0	16.0	41.0
35	33.11685073765763	35.0	35.0	40.0	15.0	41.0
36	32.93207368334295	35.0	34.0	40.0	12.0	41.0
37	32.847298689524436	35.0	34.0	40.0	12.0	41.0
38	32.80963075908679	35.0	34.0	40.0	11.0	41.0
39	32.690952361328605	35.0	33.0	40.0	10.0	41.0
40	32.63707244704525	35.0	33.0	40.0	10.0	41.0
41	32.46776147696365	35.0	33.0	40.0	10.0	41.0
42	32.29243797906536	35.0	33.0	40.0	10.0	41.0
43	32.06864749031567	35.0	33.0	40.0	10.0	41.0
44	31.90686969422237	35.0	33.0	40.0	8.0	41.0
45	31.790031319541747	35.0	33.0	40.0	8.0	41.0
46	31.66152229456853	35.0	33.0	40.0	8.0	41.0
47	31.525504821561032	35.0	32.0	40.0	8.0	41.0
48	31.467215445479273	35.0	32.0	40.0	7.0	41.0
49	31.37388733206956	35.0	31.0	39.0	7.0	41.0
50	31.270201104425947	35.0	31.0	39.0	7.0	41.0
51	30.899905217176297	35.0	30.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	2.0
16	9.0
17	33.0
18	114.0
19	254.0
20	481.0
21	788.0
22	1342.0
23	2398.0
24	5019.0
25	10603.0
26	16795.0
27	19613.0
28	16232.0
29	12363.0
30	10401.0
31	9992.0
32	10813.0
33	13600.0
34	28558.0
35	69030.0
36	71803.0
37	23567.0
38	42641.0
39	118858.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.895120745075413	13.739182395120745	17.450960191214044	48.914736668589796
2	51.73452567378225	14.861946756779032	19.023118766999094	14.380408802439629
3	16.082584686392483	15.25488337591692	54.55287233165747	14.109659606033132
4	16.8513558064782	17.420258798318635	52.27231517349378	13.456070221709387
5	14.525673782246765	21.347976592763537	50.280021429160136	13.846328195829555
6	54.29345586417209	1.364666611720102	41.83054479518668	2.5113327289211242
7	55.73436083408885	1.0102612709140362	39.6670238193357	3.588354075661419
8	49.570592598697765	2.0596719690101373	43.42825352344845	4.94148190884365
9	28.873732794857	15.119714827330421	45.75517184538037	10.25138053243221
10	24.574713591032722	13.021511579988463	54.233289376081764	8.170485452897058
11	22.552542652270667	12.566141926976016	52.62136322426441	12.259952196488914
12	12.344844638588972	23.35407566141927	53.36375999340641	10.937319706585345
13	8.64316327371631	25.28105167724388	54.03816038902167	12.037624660018134
14	7.273963570427759	16.784801780268687	63.241366521058275	12.699868128245281
15	7.762507211736586	12.904269348059014	64.15560867056787	15.177614769636527
16	8.204895738893926	15.780515948240337	53.00214291601417	23.01244539685156
17	11.074960850572818	15.288881562680293	57.14435836149345	16.49179922525344
18	13.51994560290118	18.87002390175554	53.59185691914613	14.01817357619715
19	15.028640896727932	16.320571993736092	52.439833511909676	16.21095359762631
20	10.767946921618726	21.079493942141266	53.441440698920296	14.711118437319707
21	12.10253028929366	17.035152064617158	52.167023819335704	18.69529382675348
22	9.678150498640074	20.0811835489986	50.2210912387703	20.01957471359103
23	9.208151322838539	16.149962911068986	61.083820984092974	13.558064781999505
24	11.191172834418527	16.361987966702383	56.88494189400808	15.561897304871014
25	8.80450012362977	21.815503173164096	53.00688205719938	16.37311464600676
26	9.688452979477457	20.225624330338746	53.26217753234979	16.823745157834004
27	11.58740624742438	17.68029341465425	57.19772521223111	13.534575125690266
28	8.488420011538778	16.03766586994148	55.56643039643946	19.907483722080276
29	11.110401384653425	18.58546938102695	55.08530454133356	15.218824692986072
30	16.98570015659771	17.38007912305283	49.57038654908102	16.06383417126844
31	16.86866397428501	17.85770213467403	50.76897716970247	14.504656721338499
32	17.02340723646254	16.86577927965054	50.505027610648646	15.605785873238275
33	12.067089755213054	16.71536305942471	51.86866397428501	19.34888321107723
34	15.783400642874804	17.05678727437567	49.29819500535729	17.861617077392236
35	19.52794032803099	16.346122146212807	48.78348306272151	15.342454463034699
36	17.733866315008655	19.95054809198055	47.46105662243468	14.854528970576114
37	16.015000412099234	19.82959696694964	49.55287233165746	14.60253028929366
38	15.607846369405753	20.6667765597956	47.49855765268276	16.22681941811588
39	20.1800873650375	16.9955905382016	47.73510261270914	15.08921948405176
40	13.806560619797247	15.556952114069066	50.5138877441688	20.12259952196489
41	15.941646748537048	18.143699002719853	44.952402538531274	20.962251710211817
42	16.23691584933652	16.959119756037254	46.89153548174401	19.91242891288222
43	17.91539602736339	19.12428912882222	45.76217753234979	17.1981373114646
44	13.739182395120745	23.146583697354323	43.61101953350367	19.503214374021262
45	16.26493859721421	19.874103684167146	45.41601417621363	18.444943542405014
46	18.61967361740707	17.629193109700818	45.20872826176544	18.54240501112668
47	14.932415725706749	16.45409214538861	49.52031649221132	19.093175636693317
48	16.095977911481086	14.8333058600511	46.96756779032391	22.103148438143904
49	16.52950630511827	18.71713508612874	47.01537130140938	17.73798730734361
50	14.81785213879502	17.85873238275777	46.445644111101956	20.877771367345257
51	14.341053325640814	15.488543641308826	46.61584109453557	23.554561938514794
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	103.0
1	551.5
2	1000.0
3	46090.0
4	91180.0
5	50502.5
6	9825.0
7	9567.5
8	9310.0
9	9954.5
10	10599.0
11	10690.5
12	10782.0
13	10149.0
14	9516.0
15	8610.0
16	7704.0
17	7042.5
18	6381.0
19	5677.0
20	4973.0
21	4595.5
22	4218.0
23	4001.0
24	3784.0
25	3560.0
26	3321.5
27	3307.0
28	3145.5
29	2984.0
30	3795.5
31	4607.0
32	4650.5
33	4694.0
34	5823.5
35	6953.0
36	7901.0
37	8849.0
38	8806.0
39	8763.0
40	11708.0
41	14653.0
42	20043.5
43	25434.0
44	27913.5
45	30393.0
46	33748.5
47	37104.0
48	42094.0
49	47084.0
50	45066.0
51	43048.0
52	34373.5
53	25699.0
54	20525.0
55	15351.0
56	12859.0
57	10367.0
58	8884.5
59	7402.0
60	5930.5
61	4459.0
62	3859.5
63	3260.0
64	2884.0
65	2508.0
66	1980.0
67	1452.0
68	1264.0
69	1076.0
70	985.5
71	895.0
72	837.5
73	780.0
74	770.0
75	559.5
76	359.0
77	280.5
78	202.0
79	140.5
80	79.0
81	69.0
82	59.0
83	37.5
84	16.0
85	12.5
86	9.0
87	4.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	485320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.729471057630235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4414330400077	27.4697645861487
2	8.543573736036118	5.763404461167201
3	2.8829444727358067	2.917205764616923
4	1.3682474803101143	1.8460105514718197
5	0.830623086988601	1.4008238686190746
6	0.5775060672319495	1.1687384508183538
7	0.4506569205745705	1.064029369160852
8	0.3380607516822889	0.9122088271670787
9	0.2831919743784734	0.8596723953196692
>10	2.717197314207919	20.65182240793497
>50	0.47468972516863767	10.605295300717914
>100	0.0833003904812061	4.928505954999154
>500	0.0049000229694827115	1.1480427394336978
>1k	0.003062514355926695	1.7618334500432924
>5k	0.0	0.0
>10k+	6.125028711853389E-4	17.5026418723813
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84720	17.45652353086623	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	2646	0.5452072859144482	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	1976	0.40715404269348066	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1514	0.3119591197560373	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC	1319	0.2717794444902332	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG	1073	0.2210912387702959	No Hit
CGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT	990	0.20398912058023572	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	875	0.18029341465424875	No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	799	0.16463364378142256	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	633	0.13042940740130224	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	608	0.1252781669826094	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	593	0.12218742273139371	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC	548	0.11291518997774665	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG	511	0.10529135415808127	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4166323250638754	0.0
2	0.0	0.0	0.0	1.5593834995466909	0.0
3	0.0	0.0	0.0	2.5309074425121567	0.0
4	0.0	0.0	0.0	3.2681529712354735	0.0
5	0.0	0.0	0.0	6.0582708316162535	0.0
6	0.0	0.0	0.0	6.572776724635292	0.0
7	0.0	0.0	0.0	8.282370394791066	0.0
8	0.0	0.0	0.0	10.42528640896728	0.0
9	0.0	0.0	0.0	11.807467238110936	0.0
10	0.0	0.0	0.0	12.992252534410285	0.0
11	0.0	0.0	0.0	14.226283689112337	0.0
12	0.0	0.0	0.0	15.105703453391577	0.0
13	0.0	0.0	0.0	15.470411275035028	0.0
14	0.0	0.0	0.0	15.74589961262672	0.0
15	0.0	0.0	0.0	16.559177449929944	0.0
16	0.0	0.0	0.0	17.496291106898543	0.0
17	0.0	0.0	0.0	18.611225583120415	0.0
18	0.0	0.0	0.0	19.126555674606447	0.0
19	0.0	0.0	0.0	19.72677820819253	0.0
20	0.0	0.0	0.0	20.32720679139537	0.0
21	4.120992334954257E-4	0.0	0.0	20.868911233825106	0.0
22	0.0010302480837385641	0.0	0.0	21.464394626225996	0.0
23	0.0012362977004862772	0.0	0.0	21.899571416797166	0.0
24	0.002678645017720267	0.0	0.0	22.270460726943046	0.0
25	0.002678645017720267	0.0	0.0	22.615799884612215	0.0
26	0.002678645017720267	0.0	0.0	22.978653259704938	0.0
27	0.002678645017720267	0.0	0.0	23.318429077721916	0.0
28	0.002678645017720267	0.0	0.0	23.67736751009643	0.0
29	0.002678645017720267	0.0	0.0	24.12346493035523	0.0
30	0.002678645017720267	0.0	0.0	24.517225747960108	0.0
31	0.002678645017720267	0.0	0.0	24.81805818841177	0.0
32	0.002678645017720267	0.0	0.0	25.11621198384571	0.0
33	0.002678645017720267	0.0	0.0	25.41065688617819	0.0
34	0.002678645017720267	0.0	0.0	25.820283524272647	0.0
35	0.002678645017720267	0.0	0.0	26.121115964724307	0.0
36	0.002678645017720267	0.0	0.0	26.4233907524932	0.0
37	0.0030907442512156926	0.0	0.0	26.713096513640483	0.0
38	0.0030907442512156926	0.0	0.0	27.01248660677491	0.0
39	0.003914942718206544	0.0	0.0	27.312288799142834	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAA	20	7.0292526E-4	45.000004	26
TCACGAC	45	3.8380676E-10	45.000004	24
CTTAAGG	35	1.2099918E-7	45.000004	1
GTCATGG	35	1.2099918E-7	45.000004	1
GTCATAA	20	7.0292526E-4	45.000004	44
AGGTATG	20	7.0292526E-4	45.000004	14
TGTCAGG	30	2.1629003E-6	45.000004	1
CCTTAGC	20	7.0292526E-4	45.000004	42
CTCACGA	45	3.8380676E-10	45.000004	23
TCGCAGG	35	1.2099918E-7	45.000004	1
GCCCACA	40	6.8012014E-9	45.000004	12
ACCGGGT	20	7.0292526E-4	45.000004	3
AGATCGT	20	7.0292526E-4	45.000004	42
ACCGGCC	30	2.1629003E-6	45.000004	20
TGCCCAC	40	6.8012014E-9	45.000004	11
GATAATC	20	7.0292526E-4	45.000004	8
TTAATCG	30	2.1629003E-6	45.000004	19
ATAGGTG	90	0.0	45.000004	23
TTTACGG	60	0.0	45.000004	1
CGACGGT	45	3.8380676E-10	45.000004	27
>>END_MODULE