FastQCFastQC Report
Thu 26 May 2016
SRR1043232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043232_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences796308
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT572647.191187329525761No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT58330.7325055129422284TruSeq Adapter, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT50830.638320850726101TruSeq Adapter, Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT38930.48888118667651215TruSeq Adapter, Index 13 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC31190.39168261526946857TruSeq Adapter, Index 8 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG20320.2551776448308946No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT19120.2401080988763142TruSeq Adapter, Index 13 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT17660.22177348463157473TruSeq Adapter, Index 8 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC13660.17154166478297342No Hit
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC13610.1709137670348659TruSeq Adapter, Index 8 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT13000.16325341450795422No Hit
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC12800.16074182351552413TruSeq Adapter, Index 13 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC10590.1329887430491719TruSeq Adapter, Index 8 (95% over 23bp)
CGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG10550.13248642485068593TruSeq Adapter, Index 8 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT10530.13223526575144293No Hit
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT10460.1313562089040924TruSeq Adapter, Index 8 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG10080.12658418601847526TruSeq Adapter, Index 8 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC8060.10121711699493162TruSeq Adapter, Index 8 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC8040.10096595789568862TruSeq Adapter, Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG351.01903424E-746.0000041
ATTACGG700.046.0000041
ATACTGG351.01903424E-746.0000041
TCATCGG351.01903424E-746.0000041
TCGCCCA206.310282E-446.045
CGGGTAA501.6370905E-1146.05
CGGAACG206.310282E-446.034
ACTGCGG800.046.01
TCCGCGG800.046.01
TTTACGG301.860677E-646.01
CGACGGT253.416204E-546.027
GACGTCC253.416204E-546.034
GGCGTGC301.860677E-646.07
CGGCACT206.310282E-446.039
GATCCGG206.310282E-446.01
TCGGGTA253.416204E-546.04
ATAGCGG950.046.01
CACGCGG405.6079443E-946.01
GAATCCG253.416204E-546.08
AAGCGTA301.860677E-646.017
Produced by FastQC (version 0.11.5)