FastQCFastQC Report
Thu 26 May 2016
SRR1043231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043231_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences782653
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529066.759828429712784No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC55220.7055489469790571TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT49020.6263312093609812TruSeq Adapter, Index 8 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC39070.49919951753842384TruSeq Adapter, Index 13 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG30160.38535596234857594TruSeq Adapter, Index 8 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC19480.24889702077421283TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC18280.2335645554287788No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC17270.22065973042970513No Hit
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG13930.17798436855158034TruSeq Adapter, Index 8 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG12850.16418514974068968TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT12660.16175750939432929No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT12620.16124642721614815No Hit
CGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT11050.14118645172253858TruSeq Adapter, Index 8 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT10800.1379921881089065TruSeq Adapter, Index 8 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG10760.13748110593072538TruSeq Adapter, Index 8 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC10610.13556454776254612No Hit
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC9760.12470405147619698TruSeq Adapter, Index 8 (95% over 23bp)
TGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT8980.11473794900166485TruSeq Adapter, Index 13 (96% over 25bp)
TGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG8450.1079661101407648TruSeq Adapter, Index 8 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG7860.10042764801259307TruSeq Adapter, Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGT253.8896604E-545.00000431
TCGCAGT253.8896604E-545.00000440
CAACGGC253.8896604E-545.0000042
CCTACGG253.8896604E-545.0000041
CAATTGG253.8896604E-545.0000041
GCGCATT253.8896604E-545.0000047
CTAAACT253.8896604E-545.00000434
CTACGGC253.8896604E-545.0000042
TTACCGG502.1827873E-1145.0000041
TTCGATA253.8896604E-545.00000410
CCGCACT650.045.00000433
CCCGCAC650.045.00000432
TGATCGG351.211265E-745.01
GCATAAC207.031905E-445.08
CAACGCA750.045.016
ACACCCG207.031905E-445.028
TAGCGTA351.211265E-745.032
ATTACGG700.045.01
GAATCTA207.031905E-445.08
CGACCCC207.031905E-445.019
Produced by FastQC (version 0.11.5)