##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043231_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	782653
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.104692628789515	33.0	31.0	34.0	30.0	34.0
2	32.401092182614775	34.0	31.0	34.0	30.0	34.0
3	32.454599931259445	34.0	31.0	34.0	31.0	34.0
4	36.05281012147146	37.0	35.0	37.0	35.0	37.0
5	36.00497410729915	37.0	35.0	37.0	35.0	37.0
6	36.27110737453252	37.0	37.0	37.0	35.0	37.0
7	36.20713521828959	37.0	37.0	37.0	35.0	37.0
8	36.104354036846466	37.0	37.0	37.0	35.0	37.0
9	37.642301249723694	39.0	38.0	39.0	35.0	39.0
10	37.46586290476112	39.0	37.0	39.0	35.0	39.0
11	37.17210820120794	39.0	37.0	39.0	34.0	39.0
12	37.17163545019312	39.0	37.0	39.0	33.0	39.0
13	37.1897098714245	39.0	37.0	39.0	34.0	39.0
14	38.5929026017916	40.0	38.0	41.0	34.0	41.0
15	38.72085202509925	40.0	38.0	41.0	35.0	41.0
16	38.78029599324349	40.0	38.0	41.0	35.0	41.0
17	38.73686167433077	40.0	38.0	41.0	35.0	41.0
18	38.48035975074522	40.0	38.0	41.0	35.0	41.0
19	38.20722337996532	40.0	37.0	41.0	34.0	41.0
20	37.90799881940017	40.0	35.0	41.0	34.0	41.0
21	37.826105566579315	40.0	35.0	41.0	34.0	41.0
22	37.79171229139862	40.0	35.0	41.0	34.0	41.0
23	37.74572639471132	39.0	35.0	41.0	34.0	41.0
24	37.62862469063557	39.0	35.0	41.0	34.0	41.0
25	37.57755608168627	39.0	35.0	41.0	34.0	41.0
26	37.56953848001605	39.0	35.0	41.0	34.0	41.0
27	37.494234354177394	39.0	35.0	41.0	34.0	41.0
28	37.455595263801456	39.0	35.0	41.0	33.0	41.0
29	37.406531374696065	39.0	35.0	41.0	33.0	41.0
30	37.221064763055914	39.0	35.0	41.0	33.0	41.0
31	37.09436238026303	39.0	35.0	41.0	33.0	41.0
32	36.94989733636746	39.0	35.0	41.0	33.0	41.0
33	36.67807061366915	39.0	35.0	41.0	31.0	41.0
34	36.47676556532716	39.0	35.0	41.0	31.0	41.0
35	36.30158831563924	39.0	35.0	41.0	30.0	41.0
36	36.241430110150986	39.0	35.0	41.0	30.0	41.0
37	36.162550964475955	39.0	35.0	41.0	30.0	41.0
38	36.16561745754504	39.0	35.0	41.0	30.0	41.0
39	36.11848290366229	39.0	35.0	41.0	30.0	41.0
40	36.08722639535017	39.0	35.0	41.0	30.0	41.0
41	36.05595838768905	39.0	35.0	41.0	30.0	41.0
42	35.94598755770437	39.0	35.0	41.0	30.0	41.0
43	35.787638966438514	38.0	35.0	40.0	29.0	41.0
44	35.664385110642904	38.0	35.0	40.0	29.0	41.0
45	35.631649019424955	38.0	35.0	40.0	29.0	41.0
46	35.577837176884266	38.0	35.0	40.0	28.0	41.0
47	35.43864522336208	38.0	35.0	40.0	27.0	41.0
48	35.42695038541985	38.0	35.0	40.0	27.0	41.0
49	35.34425984440103	38.0	35.0	40.0	27.0	41.0
50	35.246681479531794	38.0	35.0	40.0	26.0	41.0
51	34.84001211264762	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	6.0
15	8.0
16	20.0
17	45.0
18	113.0
19	262.0
20	491.0
21	811.0
22	1329.0
23	2091.0
24	3682.0
25	6898.0
26	10345.0
27	12406.0
28	12160.0
29	11843.0
30	13232.0
31	15340.0
32	18634.0
33	24945.0
34	43529.0
35	76793.0
36	80931.0
37	61466.0
38	107031.0
39	278190.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.51941537309638	18.781758965978536	25.64073733825846	29.05808832266662
2	33.720307722579484	19.125589501349896	26.779045119612395	20.375057656458225
3	20.595972927977023	21.236869979416166	39.001703181358785	19.165453911248022
4	22.04514644420963	24.212262650242188	36.082401779588146	17.660189125960034
5	20.28306286438562	29.146505539491958	31.55523584525965	19.01519575086277
6	72.23661060521074	1.5958541013705947	21.770184232348182	4.397351061070487
7	72.39070188193236	1.219186536051098	18.545638999658852	7.8444725823576995
8	59.90700859767995	2.973731653746935	27.130925199290107	9.988334549283016
9	34.560399052964726	16.667028683209544	29.90495149191276	18.867620771912968
10	27.063462351770195	18.53516181500614	42.30278297023074	12.098592862992923
11	23.687125712161073	14.92756048977005	41.35728094059564	20.028032857473235
12	18.355260888286377	19.11025703600446	43.57946625132721	18.95501582438194
13	12.596514675085894	22.270533684787512	43.01420936225888	22.118742277867714
14	11.01228769326892	21.63985827691199	45.762042693249754	21.585811336569336
15	11.430225144476543	18.394614216006328	48.843868227681995	21.33129241183513
16	12.754694609232956	24.278958874494826	39.962282135250234	23.004064381021987
17	14.19262431754558	22.096510203116836	42.53532536130316	21.175540118034426
18	16.30901561739366	28.802036151397875	39.090631480362305	15.798316750846162
19	16.303777025067305	23.808507729479093	36.181040639977105	23.7066746054765
20	13.164071433956046	30.56935832354824	34.89324132150519	21.373328920990527
21	13.821834197275166	24.38513619701196	35.91681115385746	25.876218451855422
22	11.703015257080725	27.749206864344732	32.15281868209794	28.3949591964766
23	11.41757586056656	23.42787927727869	42.79584950163099	22.358695360523758
24	16.26327376244645	21.612132068745662	39.59992487091981	22.52466929788808
25	12.631140492657666	24.923816812814874	38.821546713549935	23.62349598097752
26	14.998856453626319	22.363550641216477	36.707966365681855	25.929626539475347
27	20.396267566852742	23.32093533149429	37.358957290140076	18.923839811512895
28	16.915670162894667	23.094014844381867	33.30339243572823	26.686922556995246
29	19.469164495632164	22.999720182507446	30.683201878738085	26.8479134431223
30	21.831258552640826	26.23870348673039	27.56930593762498	24.36073202300381
31	23.166332972594496	20.945425367308374	28.435462459097455	27.45277920099968
32	19.867297512435268	24.43381677448371	28.19640377025323	27.502481942827796
33	17.616874911359183	27.11060968270741	29.3255120723999	25.94700333353351
34	22.69153762906422	21.390833485593234	29.443699826104293	26.47392905923826
35	24.48403059849001	23.3274516292661	32.10809899150709	20.080418780736803
36	19.710011972100023	25.198140171953597	28.343595437569398	26.748252418376982
37	17.09608217179261	23.755355182948254	36.092623423151764	23.055939222107373
38	15.512110731064723	21.25974090688977	33.67354370327591	29.554604658769595
39	20.055375754005926	18.642233531335087	35.61693368580968	25.685457028849314
40	13.828606036136065	17.90154768460608	38.68444891925285	29.58539736000501
41	18.831845019440287	20.86096903736394	32.16981216452247	28.137373778673307
42	20.20563391439118	17.91368588633788	36.79945007557628	25.081230123694663
43	24.153488199751358	21.114465797741783	33.174471956282034	21.55757404622483
44	16.768478495578503	24.434072315572802	34.73646686334813	24.060982325500575
45	19.614567375324697	21.754851767002744	34.209668908187915	24.42091194948464
46	22.997037001071995	18.337756323683678	33.08311601693215	25.58209065831218
47	17.583015717054685	16.90148763245014	37.83336932203672	27.68212732845846
48	18.87784241547659	15.297967298406828	32.429569681583025	33.39462060453356
49	20.413261049277263	15.932475822618708	38.045340655437336	25.608922472666684
50	18.691041879351385	15.011377966991757	38.96107214819339	27.336508005463468
51	15.58238453056463	14.737054607853034	38.59743717841751	31.083123683164825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	212.0
1	1081.5
2	1951.0
3	29720.0
4	57489.0
5	31654.0
6	5819.0
7	5432.0
8	5045.0
9	5142.5
10	5240.0
11	5419.5
12	5599.0
13	5494.0
14	5389.0
15	5007.5
16	4626.0
17	4203.0
18	3780.0
19	3404.5
20	3029.0
21	2789.0
22	2549.0
23	2378.0
24	2207.0
25	2182.5
26	2507.0
27	2856.0
28	2916.5
29	2977.0
30	3458.5
31	3940.0
32	4452.0
33	4964.0
34	5932.0
35	6900.0
36	8374.0
37	9848.0
38	11827.0
39	13806.0
40	16295.0
41	18784.0
42	24034.5
43	29285.0
44	33736.5
45	38188.0
46	44267.5
47	50347.0
48	68984.0
49	87621.0
50	108822.0
51	130023.0
52	127843.5
53	125664.0
54	99272.5
55	72881.0
56	54300.0
57	35719.0
58	26745.5
59	17772.0
60	13634.0
61	9496.0
62	7608.5
63	5721.0
64	4701.0
65	3681.0
66	3021.0
67	2361.0
68	1862.0
69	1363.0
70	1197.5
71	1032.0
72	954.5
73	877.0
74	658.0
75	389.0
76	339.0
77	320.5
78	302.0
79	258.5
80	215.0
81	153.0
82	91.0
83	57.5
84	24.0
85	27.5
86	31.0
87	18.0
88	5.0
89	3.0
90	1.0
91	4.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	782653.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.702814746011377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62710955316746	23.34186614448352
2	11.738540829029763	7.442895705824427
3	4.673743406719702	4.445124641808804
4	2.3356164004160322	2.961824562405416
5	1.3283887113060149	2.105683061261369
6	0.8783621059531936	1.6707930674970308
7	0.5805719714787613	1.2884035960962437
8	0.47220812246595645	1.1976261302480058
9	0.3875930354392098	1.1059011179436142
>10	3.396152292018486	23.160129484675284
>50	0.3742492912429133	8.054879639879955
>100	0.19485370683384468	10.916349248315203
>500	0.006101890610663194	1.309894848133846
>1k	0.005695097903285648	3.4634770150125593
>5k	4.067927073775463E-4	0.7121432855562762
>10k+	4.067927073775463E-4	6.823008450858447
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52906	6.759828429712784	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	5522	0.7055489469790571	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	4902	0.6263312093609812	TruSeq Adapter, Index 8 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	3907	0.49919951753842384	TruSeq Adapter, Index 13 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	3016	0.38535596234857594	TruSeq Adapter, Index 8 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1948	0.24889702077421283	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC	1828	0.2335645554287788	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1727	0.22065973042970513	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1393	0.17798436855158034	TruSeq Adapter, Index 8 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1285	0.16418514974068968	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT	1266	0.16175750939432929	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	1262	0.16124642721614815	No Hit
CGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	1105	0.14118645172253858	TruSeq Adapter, Index 8 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	1080	0.1379921881089065	TruSeq Adapter, Index 8 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1076	0.13748110593072538	TruSeq Adapter, Index 8 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC	1061	0.13556454776254612	No Hit
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	976	0.12470405147619698	TruSeq Adapter, Index 8 (95% over 23bp)
TGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT	898	0.11473794900166485	TruSeq Adapter, Index 13 (96% over 25bp)
TGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	845	0.1079661101407648	TruSeq Adapter, Index 8 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	786	0.10042764801259307	TruSeq Adapter, Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6438357739636851	0.0
2	0.0	0.0	0.0	2.273165758005144	0.0
3	0.0	0.0	0.0	3.8044957343803705	0.0
4	0.0	0.0	0.0	4.8671633533634955	0.0
5	0.0	0.0	0.0	9.994467535421188	0.0
6	0.0	0.0	0.0	11.698926599655275	0.0
7	0.0	0.0	0.0	16.72950847949219	0.0
8	0.0	0.0	0.0	22.69179317015331	0.0
9	0.0	0.0	0.0	27.54068533564683	0.0
10	0.0	0.0	0.0	30.179530392140578	0.0
11	0.0	0.0	0.0	32.384594449903084	0.0
12	0.0	0.0	0.0	33.98019301018459	0.0
13	0.0	0.0	0.0	34.64000010221643	0.0
14	0.0	0.0	0.0	35.11428436356853	0.0
15	0.0	0.0	0.0	36.55681381148478	0.0
16	0.0	0.0	0.0	38.21923636656347	0.0
17	0.0	0.0	0.0	40.13042817187183	0.0
18	0.0	0.0	0.0	41.10557296784143	0.0
19	1.277705445452838E-4	0.0	0.0	42.17910108311091	0.0
20	2.555410890905676E-4	0.0	0.0	43.32009204590029	0.0
21	2.555410890905676E-4	0.0	0.0	44.44485614953243	0.0
22	7.666232672717028E-4	0.0	0.0	45.49423563188284	0.0
23	7.666232672717028E-4	0.0	0.0	46.33764899642626	0.0
24	0.0016610170790886895	0.0	0.0	47.04281463177168	0.0
25	0.002427640346360392	0.0	0.0	47.70364388815989	0.0
26	0.002427640346360392	0.0	0.0	48.422097660138014	0.0
27	0.002427640346360392	0.0	0.0	49.04676785241991	0.0
28	0.002427640346360392	0.0	0.0	49.715007800391746	0.0
29	0.002427640346360392	0.0	0.0	50.47830903350527	0.0
30	0.002427640346360392	0.0	0.0	51.11780060895442	0.0
31	0.002427640346360392	0.0	0.0	51.7088671480209	0.0
32	0.002427640346360392	0.0	0.0	52.28064033486104	0.0
33	0.002427640346360392	0.0	0.0	52.84104194323666	0.0
34	0.002427640346360392	0.0	0.0	53.50404329888214	0.0
35	0.002427640346360392	0.0	0.0	54.00643708003419	0.0
36	0.002427640346360392	0.0	0.0	54.532979494105305	0.0
37	0.002427640346360392	0.0	0.0	55.05556102129552	0.0
38	0.002427640346360392	0.0	0.0	55.57277618561483	0.0
39	0.002427640346360392	0.0	0.0	56.0814307234496	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATAGT	25	3.8896604E-5	45.000004	31
TCGCAGT	25	3.8896604E-5	45.000004	40
CAACGGC	25	3.8896604E-5	45.000004	2
CCTACGG	25	3.8896604E-5	45.000004	1
CAATTGG	25	3.8896604E-5	45.000004	1
GCGCATT	25	3.8896604E-5	45.000004	7
CTAAACT	25	3.8896604E-5	45.000004	34
CTACGGC	25	3.8896604E-5	45.000004	2
TTACCGG	50	2.1827873E-11	45.000004	1
TTCGATA	25	3.8896604E-5	45.000004	10
CCGCACT	65	0.0	45.000004	33
CCCGCAC	65	0.0	45.000004	32
TGATCGG	35	1.211265E-7	45.0	1
GCATAAC	20	7.031905E-4	45.0	8
CAACGCA	75	0.0	45.0	16
ACACCCG	20	7.031905E-4	45.0	28
TAGCGTA	35	1.211265E-7	45.0	32
ATTACGG	70	0.0	45.0	1
GAATCTA	20	7.031905E-4	45.0	8
CGACCCC	20	7.031905E-4	45.0	19
>>END_MODULE