##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043230_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1800490
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29582002677049	33.0	31.0	34.0	31.0	34.0
2	32.63426067348333	34.0	31.0	34.0	31.0	34.0
3	32.7612755416581	34.0	31.0	34.0	31.0	34.0
4	36.19924520547185	37.0	37.0	37.0	35.0	37.0
5	36.11706757604874	37.0	37.0	37.0	35.0	37.0
6	36.291437330948796	37.0	37.0	37.0	35.0	37.0
7	36.22028170109248	37.0	37.0	37.0	35.0	37.0
8	36.19583280107082	37.0	37.0	37.0	35.0	37.0
9	37.76369988169887	39.0	38.0	39.0	35.0	39.0
10	37.59835433687496	39.0	37.0	39.0	35.0	39.0
11	37.329499191886654	39.0	37.0	39.0	35.0	39.0
12	37.31787124616077	39.0	37.0	39.0	35.0	39.0
13	37.24060172508595	39.0	37.0	39.0	34.0	39.0
14	38.66233747479853	40.0	38.0	41.0	34.0	41.0
15	38.74465395531217	41.0	38.0	41.0	35.0	41.0
16	38.66979266755161	40.0	38.0	41.0	34.0	41.0
17	38.664665174480284	40.0	38.0	41.0	34.0	41.0
18	38.44395081338969	40.0	38.0	41.0	34.0	41.0
19	38.21137301512366	40.0	37.0	41.0	34.0	41.0
20	37.954755649850874	40.0	35.0	41.0	34.0	41.0
21	37.9068959005604	40.0	35.0	41.0	34.0	41.0
22	37.838004654288554	40.0	35.0	41.0	34.0	41.0
23	37.84752983909936	40.0	35.0	41.0	34.0	41.0
24	37.753682053218846	40.0	35.0	41.0	34.0	41.0
25	37.65444184638626	40.0	35.0	41.0	34.0	41.0
26	37.56776821865159	40.0	35.0	41.0	34.0	41.0
27	37.59228876583597	40.0	35.0	41.0	34.0	41.0
28	37.54270559680976	39.0	35.0	41.0	33.0	41.0
29	37.500777566107004	40.0	35.0	41.0	33.0	41.0
30	37.41097534560036	40.0	35.0	41.0	33.0	41.0
31	37.306723169803774	40.0	35.0	41.0	33.0	41.0
32	37.10131630833828	40.0	35.0	41.0	33.0	41.0
33	36.79978672472494	39.0	35.0	41.0	32.0	41.0
34	36.573446117445805	39.0	35.0	41.0	31.0	41.0
35	36.39710745408195	39.0	35.0	41.0	31.0	41.0
36	36.31528917128115	39.0	35.0	41.0	31.0	41.0
37	36.180828552227446	39.0	35.0	41.0	30.0	41.0
38	36.093002460441326	39.0	35.0	41.0	30.0	41.0
39	36.00907253025565	39.0	35.0	41.0	30.0	41.0
40	35.89553843675888	38.0	35.0	41.0	30.0	41.0
41	35.84721048159112	38.0	35.0	41.0	30.0	41.0
42	35.802785352876164	38.0	35.0	41.0	30.0	41.0
43	35.714692111591845	38.0	35.0	40.0	30.0	41.0
44	35.55680897977773	38.0	35.0	40.0	29.0	41.0
45	35.498613710712085	38.0	35.0	40.0	29.0	41.0
46	35.49196496509283	37.0	35.0	40.0	29.0	41.0
47	35.39877588878583	37.0	35.0	40.0	29.0	41.0
48	35.29337347055524	37.0	35.0	40.0	29.0	41.0
49	35.25192419841265	37.0	35.0	40.0	29.0	41.0
50	35.150512915928445	37.0	35.0	40.0	28.0	41.0
51	35.04356147493183	36.0	35.0	40.0	28.0	41.0
52	34.67602541530361	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	2.0
13	4.0
14	8.0
15	35.0
16	75.0
17	157.0
18	334.0
19	684.0
20	1253.0
21	2025.0
22	3266.0
23	5182.0
24	8774.0
25	15109.0
26	22277.0
27	27153.0
28	28593.0
29	28788.0
30	30373.0
31	34318.0
32	41580.0
33	54995.0
34	116555.0
35	162713.0
36	165376.0
37	173524.0
38	261779.0
39	614515.0
40	1039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.467761553799242	16.971046770601337	23.34036845525385	29.220823220345572
2	34.39213769584946	19.938016873184523	24.088164888447032	21.58168054251898
3	27.214758204710936	20.80850212997573	32.281823281440055	19.694916383873277
4	23.937539225433078	23.362362467994824	30.656154713439122	22.04394359313298
5	22.025448627873523	31.27970719082028	27.830923804075557	18.86392037723064
6	77.47157718176719	2.28954340207388	17.040694477614426	3.1981849385445074
7	77.11761798177163	2.2563302212175578	15.412137806930334	5.213913990080478
8	67.4133708046143	3.983859949236041	21.194785863848175	7.4079833823014845
9	40.00766458019761	15.633244283500602	25.517331393120763	18.841759743181022
10	26.45796422085099	16.474293109098078	36.06518225594144	21.002560414109492
11	22.949419324739377	13.898660920082866	40.0057206649301	23.146199090247656
12	19.672089264589086	15.544546206865908	43.329538070192	21.453826458353003
13	16.087120728246198	19.05792312092819	44.14437180989619	20.710584340929415
14	15.525107054190803	17.8733566973435	43.464667951502086	23.136868296963602
15	18.82476436969936	14.911718476636915	42.957805930607776	23.305711223055948
16	18.71468322512205	19.26803259112797	37.438086298729786	24.57919788502019
17	19.579836600036657	19.182555859793723	37.58571277818816	23.65189476198146
18	21.1674599692306	23.56736221806286	35.271065098945286	19.994112713761254
19	20.529855761487152	20.534854400746465	33.570305861182234	25.364983976584156
20	19.57478242034113	24.678448644535656	35.92349860315803	19.823270331965187
21	19.15600753128315	18.88197101900038	35.80597504012796	26.1560464095885
22	18.827708012818732	21.760020883204017	33.32209565173925	26.090175452238
23	19.4002743697549	20.39128237313176	37.10806502674272	23.100378230370623
24	23.956978378108182	18.501685652239114	33.34458952840615	24.19674644124655
25	18.11790123799632	20.43876944609523	34.885447850307415	26.557881465601028
26	18.465973151753133	18.86786374820188	35.42552305205805	27.240640047986936
27	21.815950102472105	19.35339824158979	36.031469211159184	22.79918244477892
28	20.263872612455497	21.253381024054562	33.24933768029814	25.2334086831918
29	21.70325855739271	19.350399058034203	33.769696027192595	25.176646357380488
30	23.47666468572444	20.33085437853029	32.83322873217846	23.359252203566808
31	24.094774200356568	20.051152741753633	31.419613549644822	24.434459508244977
32	25.189976062071988	18.619042593960533	30.471038439535903	25.71994290443157
33	20.338907741781405	21.16179484473671	35.05051402673717	23.448783386744722
34	22.67554943376525	21.20806002810346	33.79202328255086	22.324367255580423
35	26.481846608423265	22.66094229904082	30.86648634538376	19.990724747152164
36	25.209859538236813	24.182139306522114	29.4349316019528	21.173069553288272
37	24.725047070519693	25.918000099972787	30.214719326405586	19.14223350310193
38	24.994584807469078	22.239445928608323	27.700126076790205	25.06584318713239
39	27.689684474781867	20.151014446067457	31.52519591888875	20.634105160261928
40	22.268715738493412	19.079528350615664	33.998911407450194	24.65284450344073
41	22.690267649362116	22.64039233764142	30.177396153269388	24.491943859727076
42	22.9826325055957	17.30817721842387	33.73803797855028	25.971152297430145
43	24.865897616759884	19.466811812339973	32.93286827474743	22.734422296152715
44	21.57368271970408	20.813334147926398	33.52387405650684	24.089109075862684
45	23.28144005243017	20.406167210037268	31.971463323872946	24.340929413659616
46	24.381529472532478	19.98317124782698	32.098039978005986	23.537259301634556
47	21.806174985698338	21.415059233875223	33.29054868396936	23.488217096457078
48	22.76585818305017	19.28741620336686	30.999894473171192	26.946831140411774
49	22.137362606845915	17.874967369993726	35.09850096362656	24.889169059533796
50	21.987792212120034	18.551616504396026	34.917716843748096	24.542874439735847
51	20.41744191858883	17.22542196846414	34.6043577026254	27.752778410321632
52	19.34623352531811	17.641364295275174	38.397325172591906	24.61507700681481
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	317.0
1	2747.0
2	5177.0
3	48984.5
4	92792.0
5	52848.5
6	12905.0
7	12073.0
8	11241.0
9	11203.0
10	11165.0
11	10831.0
12	10497.0
13	9869.5
14	8755.0
15	8268.0
16	7757.5
17	7247.0
18	6722.5
19	6198.0
20	6144.0
21	6090.0
22	5912.5
23	5735.0
24	5772.5
25	5810.0
26	6234.5
27	6659.0
28	7462.5
29	8266.0
30	8917.0
31	9568.0
32	11044.0
33	12520.0
34	14099.5
35	15679.0
36	18077.5
37	20476.0
38	23308.5
39	28985.0
40	31829.0
41	37457.5
42	43086.0
43	56951.0
44	70816.0
45	88680.5
46	106545.0
47	137743.0
48	168941.0
49	187959.5
50	206978.0
51	192453.0
52	177928.0
53	152350.0
54	126772.0
55	114098.5
56	101425.0
57	93659.0
58	85893.0
59	82061.0
60	78229.0
61	75185.0
62	72141.0
63	68494.0
64	57564.0
65	50281.0
66	42243.5
67	34206.0
68	29312.5
69	24419.0
70	21563.0
71	18707.0
72	18958.5
73	19210.0
74	15369.5
75	11529.0
76	9046.5
77	6564.0
78	5330.0
79	4096.0
80	2965.0
81	1834.0
82	1519.0
83	1204.0
84	922.5
85	641.0
86	424.5
87	208.0
88	153.5
89	73.0
90	47.0
91	27.0
92	7.0
93	8.5
94	10.0
95	7.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1800490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.82197448787487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.40572432920361	20.86875939392407
2	11.463284540472115	6.607889891454753
3	4.417844671238136	3.8199301921745836
4	2.2389277616289047	2.5812127530345226
5	1.3336809032572192	1.9219658484322741
6	0.886629477127323	1.5332647301976923
7	0.6063421102987264	1.223318378376787
8	0.4972227997430177	1.1464754279186375
9	0.4191206541593901	1.087189632137103
>10	5.134728866951588	33.710622039967305
>50	0.4478745752252756	8.44400109856505
>100	0.13542596556960138	6.836041808192811
>500	0.006596672562562306	1.3137363562421174
>1k	0.005432553875051311	2.687139706572275
>5k	9.700989062591625E-4	2.077552169033633
>10k+	1.9401978125183251E-4	4.140900573776393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74050	4.112769301690095	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9187	0.5102499875034018	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	9000	0.4998639259312742	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCTT	7656	0.4252175796588706	Illumina Single End Adapter 1 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	5933	0.3295214080611389	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	5376	0.29858538508961446	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	3749	0.208221095368483	Illumina Single End Adapter 1 (95% over 21bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	3409	0.1893373470555238	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG	2937	0.16312226116223918	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2498	0.13874000966403588	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2425	0.13468555782037112	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	2344	0.13018678248698964	Illumina Single End Adapter 1 (95% over 22bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2110	0.11719032041277652	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	2016	0.11196951940860543	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	1899	0.10547128837149887	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44060228049031097	0.0
2	0.0	0.0	0.0	1.4644346816699898	0.0
3	0.0	0.0	0.0	2.3354753428233423	0.0
4	0.0	0.0	0.0	2.9397552888380387	0.0
5	0.0	0.0	0.0	5.8514071169514965	0.0
6	0.0	0.0	0.0	6.644302384350927	0.0
7	0.0	0.0	0.0	9.198051641497592	0.0
8	0.0	0.0	0.0	11.908202767024532	0.0
9	0.0	0.0	0.0	14.442401790623663	0.0
10	0.0	0.0	0.0	15.940382895767263	0.0
11	0.0	0.0	0.0	17.392209898416542	0.0
12	0.0	0.0	0.0	18.485356763992023	0.0
13	0.0	0.0	0.0	18.993162972301985	0.0
14	0.0	0.0	0.0	19.310687646140774	0.0
15	0.0	0.0	0.0	20.295586201534025	0.0
16	0.0	0.0	0.0	21.376958494632017	5.554043621458603E-5
17	0.0	0.0	0.0	22.695488450366287	5.554043621458603E-5
18	0.0	0.0	0.0	23.433954090275424	5.554043621458603E-5
19	0.0	0.0	0.0	24.260617942893322	5.554043621458603E-5
20	0.0	0.0	0.0	25.153097212425507	5.554043621458603E-5
21	0.0	0.0	0.0	25.98575943215458	5.554043621458603E-5
22	1.6662130864375808E-4	0.0	0.0	26.780376453076663	5.554043621458603E-5
23	1.6662130864375808E-4	0.0	0.0	27.4716327222034	5.554043621458603E-5
24	6.664852345750323E-4	0.0	0.0	28.04131097645641	5.554043621458603E-5
25	8.331065432187904E-4	0.0	0.0	28.615543546479014	5.554043621458603E-5
26	8.331065432187904E-4	0.0	0.0	29.194663674888503	5.554043621458603E-5
27	8.331065432187904E-4	0.0	0.0	29.786946886680848	5.554043621458603E-5
28	9.441874156479625E-4	0.0	0.0	30.408944231847997	5.554043621458603E-5
29	9.997278518625486E-4	0.0	0.0	30.989175168981777	5.554043621458603E-5
30	9.997278518625486E-4	0.0	0.0	31.60772900710362	5.554043621458603E-5
31	9.997278518625486E-4	0.0	0.0	32.148415153652614	5.554043621458603E-5
32	9.997278518625486E-4	0.0	0.0	32.76563602130531	5.554043621458603E-5
33	0.0010552682880771345	0.0	0.0	33.333814683780524	5.554043621458603E-5
34	0.0010552682880771345	0.0	0.0	33.96642025226466	5.554043621458603E-5
35	0.0010552682880771345	0.0	0.0	34.50405167482185	1.1108087242917206E-4
36	0.0010552682880771345	0.0	0.0	35.07961721531361	1.1108087242917206E-4
37	0.0010552682880771345	0.0	0.0	35.68411932307316	1.1108087242917206E-4
38	0.0010552682880771345	0.0	0.0	36.46907230809391	1.1108087242917206E-4
39	0.0010552682880771345	0.0	0.0	37.183933262611845	1.1108087242917206E-4
40	0.0010552682880771345	0.0	0.0	37.8615265844298	1.1108087242917206E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTC	25	3.4179327E-5	46.0	13
AACCTCG	25	3.4179327E-5	46.0	27
GCTAACG	20	6.312413E-4	46.0	30
TACGTCA	25	3.4179327E-5	46.0	14
CGTTTTT	17570	0.0	45.20148	1
TACCGGT	95	0.0	43.578945	40
CGGGCAA	160	0.0	43.125	5
ATAACGG	80	0.0	43.125	1
ATTACGG	145	0.0	42.827587	1
TAGTCAG	430	0.0	42.790695	33
CGACGGT	365	0.0	42.219177	27
TCGGGTA	60	1.8189894E-12	42.166668	4
TTACGGG	535	0.0	42.13084	2
TACGGGA	295	0.0	42.101692	3
TAGTAGG	425	0.0	41.670586	1
AGGGTAA	635	0.0	41.653545	5
GCATAGG	370	0.0	41.64865	1
TCCGTAC	105	0.0	41.61905	46
CCAACGG	105	0.0	41.61905	1
GTACGGG	420	0.0	41.61905	2
>>END_MODULE