##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043229_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1762260
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27805034444407	33.0	31.0	34.0	31.0	34.0
2	32.56895634015412	34.0	31.0	34.0	31.0	34.0
3	32.67921248850907	34.0	31.0	34.0	31.0	34.0
4	36.169762123636694	37.0	37.0	37.0	35.0	37.0
5	36.13382191050129	37.0	37.0	37.0	35.0	37.0
6	36.30789100359765	37.0	37.0	37.0	35.0	37.0
7	36.250348983691396	37.0	37.0	37.0	35.0	37.0
8	36.15179031470952	37.0	37.0	37.0	35.0	37.0
9	37.73235163937217	39.0	38.0	39.0	35.0	39.0
10	37.54966406773121	39.0	37.0	39.0	35.0	39.0
11	37.32224075902534	39.0	37.0	39.0	35.0	39.0
12	37.323603781507835	39.0	37.0	39.0	35.0	39.0
13	37.25833361705991	39.0	37.0	39.0	34.0	39.0
14	38.67605971877022	40.0	38.0	41.0	35.0	41.0
15	38.768965986857786	40.0	38.0	41.0	35.0	41.0
16	38.73115998774301	40.0	38.0	41.0	35.0	41.0
17	38.703274204714404	40.0	38.0	41.0	35.0	41.0
18	38.507191901308545	40.0	38.0	41.0	35.0	41.0
19	38.29717011110733	40.0	37.0	41.0	34.0	41.0
20	38.06724830615233	40.0	35.0	41.0	34.0	41.0
21	38.01884852405434	40.0	35.0	41.0	34.0	41.0
22	37.96420732468535	40.0	35.0	41.0	34.0	41.0
23	37.93864072270834	40.0	35.0	41.0	34.0	41.0
24	37.85160986460568	40.0	35.0	41.0	34.0	41.0
25	37.82468024014618	40.0	35.0	41.0	34.0	41.0
26	37.79267474719962	40.0	35.0	41.0	34.0	41.0
27	37.77224416374428	40.0	35.0	41.0	34.0	41.0
28	37.68209174582638	40.0	35.0	41.0	34.0	41.0
29	37.65974487306073	40.0	35.0	41.0	34.0	41.0
30	37.52524712584976	40.0	35.0	41.0	34.0	41.0
31	37.409579744192115	40.0	35.0	41.0	33.0	41.0
32	37.215291160214726	40.0	35.0	41.0	33.0	41.0
33	36.899555684178274	39.0	35.0	41.0	32.0	41.0
34	36.63537049016604	39.0	35.0	41.0	31.0	41.0
35	36.45630553947772	39.0	35.0	41.0	31.0	41.0
36	36.358156004221854	39.0	35.0	41.0	31.0	41.0
37	36.18130412084482	39.0	35.0	41.0	30.0	41.0
38	36.089678594532025	39.0	35.0	41.0	30.0	41.0
39	35.95863039506089	38.0	35.0	41.0	30.0	41.0
40	35.815806407681045	38.0	35.0	40.0	30.0	41.0
41	35.72707772973341	38.0	35.0	40.0	30.0	41.0
42	35.67085163369764	38.0	35.0	40.0	30.0	41.0
43	35.570109972421776	38.0	35.0	40.0	29.0	41.0
44	35.47122274806215	38.0	35.0	40.0	29.0	41.0
45	35.438590786830545	37.0	35.0	40.0	29.0	41.0
46	35.372161883036554	37.0	35.0	40.0	29.0	41.0
47	35.215476149943825	37.0	35.0	40.0	28.0	41.0
48	35.142779726033616	37.0	35.0	40.0	28.0	41.0
49	35.08483481438607	37.0	35.0	40.0	28.0	41.0
50	35.01384699193082	36.0	35.0	40.0	27.0	41.0
51	34.61604644036635	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	2.0
13	6.0
14	6.0
15	28.0
16	64.0
17	135.0
18	313.0
19	594.0
20	998.0
21	1819.0
22	2972.0
23	4574.0
24	7333.0
25	13095.0
26	20461.0
27	26286.0
28	28610.0
29	28652.0
30	29554.0
31	33322.0
32	39698.0
33	52330.0
34	114013.0
35	159318.0
36	157685.0
37	171838.0
38	268880.0
39	599561.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.78433375324867	17.034660038813794	23.388035817643253	28.792970390294283
2	34.21339643412436	19.978550270675154	24.189449910909854	21.618603384290626
3	27.421719837027453	20.785752386140523	31.929567714185193	19.862960062646827
4	24.140081486273306	23.416238239533328	30.30636795932496	22.137312314868407
5	22.157513647248418	31.481449956306108	27.418825825928067	18.942210570517403
6	77.92357540885	2.254775118313983	16.63398136483833	3.187668107997685
7	77.51052625605756	2.2571584215722993	14.993133816803422	5.239181505566715
8	67.77966928830025	3.9372737280537495	20.86950847207563	7.41354851157037
9	40.2572265159511	15.654500470986122	25.11127756403709	18.976995449025683
10	26.830036430492665	16.527016444792483	35.602181289934514	21.040765834780338
11	23.146811480712266	13.90169441512603	39.74572424046395	23.20576986369775
12	19.853143123035196	15.46372271968949	43.10850839263219	21.57462576464313
13	16.25026954024945	19.011269619692893	43.923087399135206	20.815373440922453
14	15.66777887485388	17.913134270765948	43.17955352785627	23.239533326523894
15	19.002417350447722	14.864321950223008	42.8250655408396	23.308195158489667
16	18.9125327704198	19.2108996402347	37.197575840114396	24.678991749231102
17	19.811492061330338	19.115737745849078	37.367982023084	23.704788169736588
18	21.34475049084698	23.587211875659662	35.03921101313087	20.028826620362487
19	20.60411063066744	20.603145960300978	33.26177749026818	25.530965918763403
20	19.731764892808098	24.72433125645478	35.63713640438982	19.906767446347303
21	19.216006718645374	18.953616378967915	35.57250348983692	26.25787341254979
22	18.856468398533703	21.806657360434897	33.049833736225075	26.28704050480633
23	19.573048244867387	20.428256897393123	36.82549680523873	23.173198052500766
24	24.037202228956	18.476501764779318	33.10703301442466	24.379262991840022
25	18.053238455165527	20.454473233234598	34.81234324106545	26.679945070534426
26	18.533474061716205	18.76130650414808	35.30279300443748	27.402426429698227
27	21.856990455437902	19.38493752340744	35.840908832975835	22.917163188178815
28	20.301714843439676	21.36285224654705	32.983952424727335	25.351480485285936
29	21.755019123171383	19.373872186850978	33.50390975224995	25.367198937727693
30	23.59368084164652	20.23884103367267	32.61227060706139	23.55520751761942
31	24.28824350549862	19.98740253992033	31.13257975554118	24.59177419903987
32	25.298991068287314	18.501129231781917	30.25319759853824	25.946682101392533
33	20.342060762884024	21.130366688229888	34.88452328260302	23.643049266283068
34	22.81973148116623	21.13677890890107	33.622450716693336	22.421038893239363
35	26.667574591717454	22.60744725522908	30.693371012222944	20.031607140830523
36	25.290479270936185	24.198245434839354	29.340676177181575	21.170599117042887
37	24.86608105500891	25.94021313540567	30.027748459364677	19.16595735022074
38	25.16921453134044	22.266294417395844	27.513136540578575	25.051354510685144
39	27.947635422695853	20.030642470464063	31.373463620578125	20.64825848626196
40	22.442885839773925	18.95526199312247	33.87337850260461	24.728473664498996
41	22.855708011303665	22.56477477784209	29.975542768944425	24.60397444190982
42	23.156912146902272	17.180325264149445	33.6583705015151	26.00439208743318
43	24.985189472609036	19.292953366699578	32.870859010588674	22.850998150102708
44	21.700089657598763	20.618921158058402	33.52882094583092	24.152168238511912
45	23.356769148706775	20.24519650902818	31.998229546150963	24.39980479611408
46	24.53372374110517	19.858420437392894	32.00918139207609	23.598674429425852
47	22.06734534064213	21.20975338485808	33.16218946125997	23.560711813239816
48	22.974589447641097	19.135258134441003	30.83648269835325	27.05366971956465
49	22.283601738676474	17.786762452759525	35.014640291443946	24.91499551712006
50	22.14253288391043	18.344001452680082	34.816485649109666	24.696980014299818
51	20.607174877713845	17.164663557023367	34.37489360253311	27.853267962729678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	258.0
1	2645.0
2	5032.0
3	46313.0
4	87594.0
5	50331.0
6	13068.0
7	11937.0
8	10806.0
9	10680.0
10	10554.0
11	10254.5
12	9955.0
13	9363.5
14	8772.0
15	8264.0
16	7756.0
17	7200.0
18	6644.0
19	6185.0
20	5726.0
21	5589.5
22	5453.0
23	5321.5
24	5190.0
25	5280.0
26	5812.5
27	6255.0
28	7245.0
29	8235.0
30	9440.5
31	10646.0
32	11727.0
33	12808.0
34	14916.0
35	17024.0
36	19381.0
37	21738.0
38	24955.5
39	28173.0
40	31964.5
41	35756.0
42	44433.0
43	53110.0
44	63931.0
45	74752.0
46	99470.0
47	124188.0
48	160980.0
49	197772.0
50	207773.5
51	217775.0
52	183181.5
53	148588.0
54	129877.5
55	111167.0
56	101211.5
57	91256.0
58	86724.5
59	82193.0
60	78322.5
61	74452.0
62	69549.0
63	64646.0
64	62556.5
65	60467.0
66	50499.0
67	40531.0
68	34350.0
69	28169.0
70	24410.5
71	20652.0
72	19864.5
73	19077.0
74	16325.0
75	10621.5
76	7670.0
77	6092.0
78	4514.0
79	3444.5
80	2375.0
81	1886.5
82	1398.0
83	1030.0
84	662.0
85	471.0
86	280.0
87	188.0
88	96.0
89	79.5
90	63.0
91	36.5
92	10.0
93	9.5
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1762260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.60634688672945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78840372373669	20.53603979365792
2	11.676976213276923	6.680712642901762
3	4.575253353295721	3.926438535571486
4	2.2720012323038947	2.5997462151344797
5	1.349854045454716	1.9307196535366338
6	0.8796440136489	1.5098041074765245
7	0.6655300338027628	1.3326868107349028
8	0.5027369569123286	1.1505174225770263
9	0.42025933594207726	1.0819875911711068
>10	5.284227620015864	34.22992956208005
>50	0.43962689929159554	8.306481039083144
>100	0.13206013399998479	6.699757407738784
>500	0.006212232654859441	1.1962127719497258
>1k	0.006011838053089781	2.7888318945024304
>5k	0.0010019730088482968	2.0309877940004233
>10k+	2.003946017696594E-4	3.999146757883585
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69762	3.9586667120629193	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9057	0.5139423240611488	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	8486	0.48154074881118564	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	7543	0.4280299161304234	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	5312	0.3014311168613031	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	5031	0.2854856831568554	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	3658	0.20757436473619104	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3322	0.18850793866966284	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	2644	0.15003461464256126	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2425	0.1376073905099134	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2400	0.13618875761805863	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	2241	0.12716625242586224	Illumina Single End Adapter 1 (95% over 21bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	1927	0.10934822330416624	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	1902	0.10792959041231145	No Hit
CGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	1807	0.10253878542326332	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	1804	0.10236854947624073	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44181902783925187	0.0
2	0.0	0.0	0.0	1.4528503172063145	0.0
3	0.0	0.0	0.0	2.311974396513568	0.0
4	0.0	0.0	0.0	2.923405172902977	0.0
5	0.0	0.0	0.0	5.852768603951744	0.0
6	0.0	0.0	0.0	6.641471746507325	0.0
7	0.0	0.0	0.0	9.245627773427303	0.0
8	0.0	0.0	0.0	12.01360752669867	0.0
9	0.0	0.0	0.0	14.62048732877101	0.0
10	0.0	0.0	0.0	16.16980468262345	0.0
11	0.0	0.0	0.0	17.630031890867407	0.0
12	0.0	0.0	0.0	18.765278676245277	0.0
13	0.0	0.0	0.0	19.303451250099304	0.0
14	0.0	0.0	0.0	19.64165333151748	0.0
15	0.0	0.0	0.0	20.64230022811617	0.0
16	0.0	0.0	0.0	21.739584397308	0.0
17	0.0	0.0	0.0	23.10118824691022	0.0
18	0.0	0.0	0.0	23.881720064008714	0.0
19	5.67453156741911E-5	0.0	0.0	24.732786308490233	0.0
20	1.134906313483822E-4	0.0	0.0	25.65909684155573	0.0
21	1.134906313483822E-4	0.0	0.0	26.508347235935673	0.0
22	2.269812626967644E-4	0.0	0.0	27.33041662410768	0.0
23	2.269812626967644E-4	0.0	0.0	28.03973307003507	0.0
24	5.67453156741911E-4	0.0	0.0	28.616435713231873	0.0
25	6.241984724161021E-4	0.0	0.0	29.19779147231396	0.0
26	6.241984724161021E-4	0.0	0.0	29.78862369911364	0.0
27	6.241984724161021E-4	0.0	0.0	30.38342809801051	0.0
28	8.511797351128664E-4	0.0	0.0	31.023685494762407	0.0
29	8.511797351128664E-4	0.0	0.0	31.6204192343922	0.0
30	8.511797351128664E-4	0.0	0.0	32.23593567351015	0.0
31	8.511797351128664E-4	0.0	0.0	32.79101835143509	0.0
32	8.511797351128664E-4	0.0	0.0	33.42900593556002	0.0
33	8.511797351128664E-4	0.0	0.0	34.01019145869508	0.0
34	8.511797351128664E-4	0.0	0.0	34.648235788135686	0.0
35	8.511797351128664E-4	0.0	0.0	35.19236662013551	0.0
36	8.511797351128664E-4	0.0	0.0	35.7772405887894	0.0
37	8.511797351128664E-4	0.0	0.0	36.38157820071953	0.0
38	8.511797351128664E-4	0.0	0.0	37.187191447346024	0.0
39	8.511797351128664E-4	0.0	0.0	37.905133181255884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	145	0.0	45.000004	1
TATGCGT	20	7.034312E-4	45.0	9
AACGCGT	20	7.034312E-4	45.0	13
GTAAGTC	20	7.034312E-4	45.0	30
TTCGATC	20	7.034312E-4	45.0	23
CGTTTTT	17340	0.0	43.98789	1
ACGGGTA	155	0.0	43.548386	4
ACGTAGG	125	0.0	43.2	1
CTTGCGG	695	0.0	43.057552	1
ACGGGAT	230	0.0	43.04348	4
CACGACG	425	0.0	42.88235	25
ATACGGG	385	0.0	42.66234	2
TACGGGC	375	0.0	42.600002	3
TATACGG	85	0.0	42.35294	1
TTACGGG	535	0.0	42.056072	2
ACTACGG	75	0.0	42.0	1
CTCACGT	350	0.0	41.785713	44
AGGGTAA	720	0.0	41.5625	5
CGGTCTA	435	0.0	41.37931	30
CTAACGG	120	0.0	41.249996	1
>>END_MODULE