FastQCFastQC Report
Thu 26 May 2016
SRR1043228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043228_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences651794
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448936.887605593178213No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT60010.9206896657532903No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT56470.8663780274135693Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT38250.5868418549418988No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC23780.3648391976606105No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT18380.2819909357864602No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG17290.2652678607044557No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC14880.22829298827543673No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC14680.22522453413194965No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT11610.1781237630294234No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC10670.16370202855503427No Hit
TCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC10540.16170753336176769No Hit
CGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG10520.16140068794741896No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT10090.1548035115389218No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG8220.12611346529731787No Hit
ATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC7950.12197105220361035No Hit
TGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC7880.12089709325338988No Hit
GGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC7710.11828890723142589No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT7560.11598756662381057No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTTG7460.11445333955206706Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC7210.11061777187270824No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT7110.10908354480096473No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC6840.1049411317072572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCC351.01865226E-746.00000416
ACCGGCC351.01865226E-746.00000420
GGTCGAG351.01865226E-746.0000047
ATTCGCC351.01865226E-746.00000413
GTTACGG700.046.0000041
ACGGCCA351.01865226E-746.0000046
GTCAAGG700.046.0000041
GATGCGT351.01865226E-746.00000425
TCACTGG700.046.0000041
AGTTCCG351.01865226E-746.00000423
GGCCTTA351.01865226E-746.00000423
AGTACGG700.046.0000041
TTTAGAG301.860155E-646.031
TAGAGTA301.860155E-646.033
GTGCAAC453.092282E-1046.033
ACTATTC206.309433E-446.046
AACCGTA453.092282E-1046.037
TCCATTG253.4155153E-546.011
ACAGTAC650.046.022
ACTGCGG900.046.01
Produced by FastQC (version 0.11.5)