##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043228_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	651794
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27354501575651	33.0	31.0	34.0	31.0	34.0
2	32.58235730921119	34.0	31.0	34.0	31.0	34.0
3	32.64960248176571	34.0	31.0	34.0	31.0	34.0
4	36.17080089721599	37.0	35.0	37.0	35.0	37.0
5	36.05588882376947	37.0	35.0	37.0	35.0	37.0
6	36.29295452244114	37.0	37.0	37.0	35.0	37.0
7	36.20322218369608	37.0	37.0	37.0	35.0	37.0
8	36.206839891131246	37.0	37.0	37.0	35.0	37.0
9	37.81215537424401	39.0	38.0	39.0	35.0	39.0
10	37.65935096057957	39.0	37.0	39.0	35.0	39.0
11	37.31352237056493	39.0	37.0	39.0	35.0	39.0
12	37.26130955485936	39.0	37.0	39.0	34.0	39.0
13	37.27067294267821	39.0	37.0	39.0	34.0	39.0
14	38.694466349797636	40.0	38.0	41.0	35.0	41.0
15	38.78176847286106	41.0	38.0	41.0	35.0	41.0
16	38.787070761621	41.0	38.0	41.0	35.0	41.0
17	38.77630509025858	40.0	38.0	41.0	35.0	41.0
18	38.51423762722578	40.0	38.0	41.0	35.0	41.0
19	38.241688324838826	40.0	37.0	41.0	35.0	41.0
20	37.94857117432809	40.0	35.0	41.0	34.0	41.0
21	37.908457273310276	40.0	35.0	41.0	34.0	41.0
22	37.86008462796528	40.0	35.0	41.0	34.0	41.0
23	37.85994961598296	40.0	35.0	41.0	34.0	41.0
24	37.77073124330693	40.0	35.0	41.0	34.0	41.0
25	37.687168031617354	39.0	35.0	41.0	34.0	41.0
26	37.648402716195605	39.0	35.0	41.0	34.0	41.0
27	37.68141314587124	39.0	35.0	41.0	34.0	41.0
28	37.69626292969865	40.0	35.0	41.0	34.0	41.0
29	37.62674249839673	40.0	35.0	41.0	34.0	41.0
30	37.56727432286888	40.0	35.0	41.0	34.0	41.0
31	37.390680184229986	40.0	35.0	41.0	34.0	41.0
32	37.23722065560591	40.0	35.0	41.0	33.0	41.0
33	37.015184245329046	40.0	35.0	41.0	33.0	41.0
34	36.758690629247894	39.0	35.0	41.0	32.0	41.0
35	36.616762351295044	39.0	35.0	41.0	32.0	41.0
36	36.5575488574611	39.0	35.0	41.0	32.0	41.0
37	36.39689533809762	39.0	35.0	41.0	31.0	41.0
38	36.399880943979234	39.0	35.0	41.0	31.0	41.0
39	36.309129878458535	39.0	35.0	41.0	31.0	41.0
40	36.24522778669334	39.0	35.0	41.0	31.0	41.0
41	36.23524763959165	39.0	35.0	41.0	31.0	41.0
42	36.21570618937885	39.0	35.0	41.0	31.0	41.0
43	36.13161520357659	39.0	35.0	41.0	31.0	41.0
44	36.003804883137924	38.0	35.0	41.0	30.0	41.0
45	35.97231026980917	38.0	35.0	41.0	30.0	41.0
46	35.98348404557268	39.0	35.0	41.0	30.0	41.0
47	35.90756128470038	38.0	35.0	41.0	30.0	41.0
48	35.88102989594872	38.0	35.0	41.0	30.0	41.0
49	35.82386459525556	38.0	35.0	40.0	30.0	41.0
50	35.71688447576995	38.0	35.0	40.0	30.0	41.0
51	35.63416202051568	38.0	35.0	40.0	29.0	41.0
52	35.337264534500164	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	2.0
15	4.0
16	15.0
17	51.0
18	92.0
19	191.0
20	351.0
21	605.0
22	1031.0
23	1771.0
24	3385.0
25	5875.0
26	8158.0
27	8562.0
28	7851.0
29	7955.0
30	9047.0
31	10831.0
32	14232.0
33	18907.0
34	34642.0
35	73958.0
36	68328.0
37	47455.0
38	76939.0
39	250983.0
40	570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.82964249440774	19.206221597621333	24.19153904454475	29.772596863426177
2	33.38846322611132	19.60466036815313	26.759835162643412	20.24704124309214
3	21.142876430283188	21.844478470191504	38.11787159746791	18.8947735020574
4	23.17496018680749	24.30399788890355	35.2585326038595	17.262509320429462
5	19.673240318260063	29.126533843514974	31.347941220692427	19.852284617532533
6	71.13704636741056	1.5329996900861316	22.77099819881742	4.558955743685889
7	70.97104299824791	1.531925731135911	18.635335704225568	8.861695566390608
8	57.82194987987002	2.80011782863911	28.61318146530959	10.764750826181277
9	32.6043197697432	18.937271591944693	30.306968152514447	18.15144048579766
10	26.154429160133418	17.22154545761391	44.55272678177461	12.071298600478066
11	23.468457825632026	14.401329254334957	40.76518040976136	21.36503251027165
12	15.680107518633188	22.022909078635276	44.69264829071762	17.60433511201392
13	10.815073474134467	25.85249327241429	43.13678861726251	20.195644636188735
14	8.681730730875092	22.21652853508931	48.91253985154819	20.189200882487413
15	8.660098129163508	18.202837092701067	51.85395999349488	21.283104784640546
16	9.509446236080725	24.471535485137945	40.70227709981988	25.31674117896145
17	12.0040994547357	22.33650509209965	43.12881063648945	22.530584816675205
18	13.951187031485407	29.51561382890913	40.15931413912985	16.37388500047561
19	14.418512597538486	23.549925283141608	36.767751774333604	25.263810344986297
20	11.062390878099523	31.7871904313326	35.49050773710712	21.659910953460756
21	12.638195503487298	24.67973009877354	35.6844340389755	26.997640358763658
22	10.144923089196894	28.343157500682732	31.676112391338368	29.835807018782006
23	9.738506337892034	23.496227335630582	44.33977606421661	22.425490262260777
24	15.51272334510597	21.685992813680397	39.971064477426914	22.830219363786718
25	11.080034489424573	25.700604792311683	39.77652448472983	23.44283623353391
26	14.094483839986252	22.529817703139337	38.2333988959702	25.142299560904213
27	20.71958318118915	22.17034830022983	40.359377349285204	16.75069116929582
28	14.961935826350043	21.563714916062438	35.699316041571414	27.775033216016105
29	16.4541250763278	21.98992319659279	37.50893687269291	24.047014854386507
30	24.42029230094171	22.23954194116546	29.54062173017855	23.79954402771428
31	18.60511143091222	21.930241763501964	37.51430666744401	21.950340138141804
32	19.359644304795687	22.60959751087	33.35302258075404	24.677735603580274
33	18.428215049540192	24.35232604166347	37.735849056603776	19.48360985219256
34	17.09435803336637	22.972595636044517	37.033019635038066	22.90002669555105
35	17.73152253626146	27.705992997787643	33.36544982003517	21.197034645915735
36	20.461833033136237	29.970358732973917	30.191440854012157	19.376367379877692
37	15.295016523625563	34.386017668158956	33.57947449654339	16.73949131167209
38	17.6379346848851	31.271229867105248	29.37492520643025	21.715910241579394
39	25.0975001304093	25.13907768405355	32.58422139510336	17.17920079043379
40	18.380807433023318	20.367324645516835	38.64150329705397	22.61036462440587
41	21.101452299346114	23.049767257753217	32.37648704958929	23.472293393311382
42	21.97764938001884	16.97806362132821	38.01277704305348	23.031509955599468
43	23.850480366496164	21.015075315206953	35.63533877267972	19.499105545617173
44	14.807592582932644	25.937182606774535	35.62337180152011	23.631853008772712
45	18.934203137801205	24.0548394124524	34.5885356416291	22.42242180811729
46	24.18218025940711	20.0325562984624	33.61077272880696	22.174490713323536
47	17.963651092216253	18.042050095582347	37.94619158813981	26.04810722406159
48	19.67707588593942	15.322172342795422	32.924666382323245	32.076085388941905
49	20.324673132922367	16.561367548642668	39.33328628370313	23.780673034731834
50	18.81698818952	16.149887848001054	39.09502081946136	25.938103143017578
51	15.763876316750384	14.957486567841988	39.03196408681271	30.246673028594923
52	14.672887446033563	14.357143514668744	47.92373050380949	23.046238535488207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	220.0
1	987.5
2	1755.0
3	24726.5
4	47698.0
5	25688.5
6	3679.0
7	3620.5
8	3562.0
9	3877.0
10	4192.0
11	4347.5
12	4503.0
13	4445.0
14	4130.0
15	3873.0
16	3523.0
17	3173.0
18	2913.5
19	2654.0
20	2491.0
21	2328.0
22	2202.5
23	2077.0
24	2149.5
25	2222.0
26	2366.0
27	2510.0
28	2509.0
29	2508.0
30	3065.5
31	3623.0
32	4435.0
33	5247.0
34	5640.0
35	6033.0
36	6997.0
37	7961.0
38	9618.5
39	14316.5
40	17357.0
41	25289.5
42	33222.0
43	50301.5
44	67381.0
45	89219.5
46	111058.0
47	112209.5
48	113361.0
49	94669.0
50	75977.0
51	58316.0
52	40655.0
53	31259.5
54	21864.0
55	17405.0
56	12946.0
57	10785.0
58	8624.0
59	7592.5
60	6561.0
61	5767.5
62	4974.0
63	4110.0
64	2783.0
65	2320.0
66	1968.0
67	1616.0
68	1431.0
69	1246.0
70	1125.5
71	1005.0
72	1112.5
73	1220.0
74	998.0
75	776.0
76	652.5
77	529.0
78	371.0
79	213.0
80	162.0
81	111.0
82	72.5
83	34.0
84	21.0
85	8.0
86	6.5
87	5.0
88	2.5
89	1.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	651794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.52143705010653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99969858315825	20.82056307816299
2	11.529056769945596	6.576505338222158
3	4.702963405375499	4.024058241461158
4	2.391552002526922	2.7284199956851127
5	1.4479201750056854	2.0648382062501947
6	0.921894827885667	1.5776259180215908
7	0.6672017257920101	1.332068641532948
8	0.5101085766276386	1.1639223725603711
9	0.38986294909752284	1.0007506404768496
>10	3.641713677036157	23.11097104786191
>50	0.5471569344442497	10.580110854674805
>100	0.23465030587826083	11.865268176681045
>500	0.00865070252085754	1.6495473810298027
>1k	0.00594735798308956	2.786358009519258
>5k	0.0010813378151071926	1.796197802583448
>10k+	5.406689075535963E-4	6.922794295276333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44893	6.887605593178213	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	6001	0.9206896657532903	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT	5647	0.8663780274135693	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	3825	0.5868418549418988	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2378	0.3648391976606105	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	1838	0.2819909357864602	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	1729	0.2652678607044557	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1488	0.22829298827543673	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1468	0.22522453413194965	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	1161	0.1781237630294234	No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1067	0.16370202855503427	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1054	0.16170753336176769	No Hit
CGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1052	0.16140068794741896	No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	1009	0.1548035115389218	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	822	0.12611346529731787	No Hit
ATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	795	0.12197105220361035	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	788	0.12089709325338988	No Hit
GGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	771	0.11828890723142589	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	756	0.11598756662381057	No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTTG	746	0.11445333955206706	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	721	0.11061777187270824	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	711	0.10908354480096473	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	684	0.1049411317072572	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8849421749816659	0.0
2	0.0	0.0	0.0	2.8772894503478095	0.0
3	0.0	0.0	0.0	4.738000042958358	0.0
4	0.0	0.0	0.0	6.011408512505485	0.0
5	0.0	0.0	0.0	11.951935734296418	0.0
6	0.0	0.0	0.0	13.595399773548085	0.0
7	0.0	0.0	0.0	19.116315891217162	0.0
8	0.0	0.0	0.0	25.599499228283783	0.0
9	0.0	0.0	0.0	30.72335737978564	0.0
10	0.0	0.0	0.0	33.375115450587145	0.0
11	0.0	0.0	0.0	35.73966621355827	0.0
12	0.0	0.0	0.0	37.45124993479535	0.0
13	0.0	0.0	0.0	38.123548237633365	0.0
14	0.0	0.0	0.0	38.58228213208468	0.0
15	0.0	0.0	0.0	40.058822265930644	0.0
16	0.0	0.0	0.0	41.812443808933494	0.0
17	0.0	0.0	0.0	44.031856690917685	0.0
18	0.0	0.0	0.0	44.94809709816292	0.0
19	0.0	0.0	0.0	46.09738659760599	0.0
20	1.5342270717435264E-4	0.0	0.0	47.13084195313243	0.0
21	4.602681215230579E-4	0.0	0.0	48.09019414109366	0.0
22	0.0010739589502204684	0.0	0.0	49.08514039711933	0.0
23	0.0010739589502204684	0.0	0.0	49.790117736585486	0.0
24	0.004295835800881873	0.0	0.0	50.400586688432234	0.0
25	0.004295835800881873	0.0	0.0	50.91946228409589	0.0
26	0.004295835800881873	0.0	0.0	51.50139461240821	0.0
27	0.004449258508056227	0.0	0.0	52.01229222729881	0.0
28	0.005216372043927989	0.0	0.0	52.52840621423333	0.0
29	0.005216372043927989	0.0	0.0	53.18704989613283	0.0
30	0.005216372043927989	0.0	0.0	53.74520170483312	0.0
31	0.005216372043927989	0.0	0.0	54.2011739905553	0.0
32	0.005216372043927989	0.0	0.0	54.64901487279723	0.0
33	0.005216372043927989	0.0	0.0	55.10176528166875	0.0
34	0.005369794751102342	0.0	0.0	55.653319913960544	0.0
35	0.005369794751102342	0.0	0.0	56.08750617526396	0.0
36	0.005369794751102342	0.0	0.0	56.47566562441508	0.0
37	0.005369794751102342	0.0	0.0	56.890520624614524	0.0
38	0.005369794751102342	0.0	0.0	57.308444078957464	0.0
39	0.005369794751102342	0.0	0.0	57.70887734468252	0.0
40	0.005369794751102342	0.0	0.0	58.102560011291914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCC	35	1.01865226E-7	46.000004	16
ACCGGCC	35	1.01865226E-7	46.000004	20
GGTCGAG	35	1.01865226E-7	46.000004	7
ATTCGCC	35	1.01865226E-7	46.000004	13
GTTACGG	70	0.0	46.000004	1
ACGGCCA	35	1.01865226E-7	46.000004	6
GTCAAGG	70	0.0	46.000004	1
GATGCGT	35	1.01865226E-7	46.000004	25
TCACTGG	70	0.0	46.000004	1
AGTTCCG	35	1.01865226E-7	46.000004	23
GGCCTTA	35	1.01865226E-7	46.000004	23
AGTACGG	70	0.0	46.000004	1
TTTAGAG	30	1.860155E-6	46.0	31
TAGAGTA	30	1.860155E-6	46.0	33
GTGCAAC	45	3.092282E-10	46.0	33
ACTATTC	20	6.309433E-4	46.0	46
AACCGTA	45	3.092282E-10	46.0	37
TCCATTG	25	3.4155153E-5	46.0	11
ACAGTAC	65	0.0	46.0	22
ACTGCGG	90	0.0	46.0	1
>>END_MODULE