SRR1043227_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043227_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	645089
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41882	6.492437477619367	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	5745	0.8905747889050969	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	5474	0.8485650817174064	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3813	0.5910812306518946	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	2490	0.3859932505437234	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1809	0.2804264217805605	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	1682	0.2607392158291337	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1563	0.242292148835277	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1533	0.23764162774438874	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	1114	0.17268934984164974	No Hit
CGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	1076	0.16679868979319132	No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1072	0.16617862031440622	No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1042	0.16152809922351802	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1005	0.15579245654475585	No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT	916	0.14199591064178743	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	867	0.13440005952666995	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	825	0.12788932999942643	No Hit
ATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	743	0.11517790568433192	No Hit
GGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	738	0.11440281883585054	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	728	0.1128526451388878	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	703	0.10897721089648095	No Hit
ATAGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	657	0.10184641189045233	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTTA	20	7.0309814E-4	45.000004	32
CTTCGCT	20	7.0309814E-4	45.000004	2
TAGAGTA	30	2.1640153E-6	45.000004	33
AACCGTA	25	3.888895E-5	45.000004	37
TTGGCTA	20	7.0309814E-4	45.000004	19
CGGAACA	30	2.1640153E-6	45.000004	27
AACCGCG	25	3.888895E-5	45.000004	19
CTGTCGG	25	3.888895E-5	45.000004	1
TCGTTGA	20	7.0309814E-4	45.000004	23
CTCACGG	50	2.1827873E-11	45.000004	1
GGTACGA	20	7.0309814E-4	45.000004	7
GTACCGG	25	3.888895E-5	45.000004	1
TCGCAGG	65	0.0	45.000004	1
ACACGAC	30	2.1640153E-6	45.000004	25
ACTGCAC	20	7.0309814E-4	45.000004	21
GGTAATC	25	3.888895E-5	45.000004	7
TTAATCG	20	7.0309814E-4	45.000004	19
CGCGGGT	30	2.1640153E-6	45.000004	3
GACACGA	30	2.1640153E-6	45.000004	24
TAGACAG	25	3.888895E-5	45.000004	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content