##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043227_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	645089
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21144834278681	33.0	31.0	34.0	30.0	34.0
2	32.48312558422171	34.0	31.0	34.0	31.0	34.0
3	32.542776268080836	34.0	31.0	34.0	31.0	34.0
4	36.11486322042385	37.0	35.0	37.0	35.0	37.0
5	36.06538632653789	37.0	35.0	37.0	35.0	37.0
6	36.295188725896736	37.0	37.0	37.0	35.0	37.0
7	36.20920369127361	37.0	37.0	37.0	35.0	37.0
8	36.13668811590339	37.0	37.0	37.0	35.0	37.0
9	37.74305870972842	39.0	38.0	39.0	35.0	39.0
10	37.59795935134532	39.0	37.0	39.0	35.0	39.0
11	37.29628624887419	39.0	37.0	39.0	35.0	39.0
12	37.24880442853622	39.0	37.0	39.0	34.0	39.0
13	37.26560831141129	39.0	37.0	39.0	34.0	39.0
14	38.69008927450321	40.0	38.0	41.0	35.0	41.0
15	38.7891577751287	40.0	38.0	41.0	35.0	41.0
16	38.82077821819935	40.0	38.0	41.0	35.0	41.0
17	38.79441131378771	40.0	38.0	41.0	35.0	41.0
18	38.5684657465869	40.0	38.0	41.0	35.0	41.0
19	38.31643075606622	40.0	37.0	41.0	35.0	41.0
20	38.0491994127942	40.0	35.0	41.0	35.0	41.0
21	38.01009318094093	40.0	35.0	41.0	35.0	41.0
22	37.96345000457301	40.0	35.0	41.0	34.0	41.0
23	37.94501378879504	40.0	35.0	41.0	34.0	41.0
24	37.85431002543835	40.0	35.0	41.0	34.0	41.0
25	37.83647992757589	40.0	35.0	41.0	34.0	41.0
26	37.830703980381	40.0	35.0	41.0	34.0	41.0
27	37.82561010961278	40.0	35.0	41.0	34.0	41.0
28	37.755987158361094	40.0	35.0	41.0	34.0	41.0
29	37.755573261984004	40.0	35.0	41.0	34.0	41.0
30	37.65264637902677	40.0	35.0	41.0	34.0	41.0
31	37.489754126949926	40.0	35.0	41.0	34.0	41.0
32	37.354802205587134	40.0	35.0	41.0	33.0	41.0
33	37.10693098161649	40.0	35.0	41.0	33.0	41.0
34	36.814131073386775	39.0	35.0	41.0	33.0	41.0
35	36.66287132473194	39.0	35.0	41.0	32.0	41.0
36	36.57652819998481	39.0	35.0	41.0	32.0	41.0
37	36.38446942979961	39.0	35.0	41.0	31.0	41.0
38	36.364968244691816	39.0	35.0	41.0	31.0	41.0
39	36.24571493235817	39.0	35.0	41.0	31.0	41.0
40	36.167914814855	39.0	35.0	40.0	31.0	41.0
41	36.114455524741544	38.0	35.0	40.0	31.0	41.0
42	36.09140444186771	39.0	35.0	40.0	31.0	41.0
43	35.98699249250878	38.0	35.0	40.0	30.0	41.0
44	35.91162149718876	38.0	35.0	40.0	30.0	41.0
45	35.903142047066375	38.0	35.0	40.0	30.0	41.0
46	35.85368840578587	38.0	35.0	40.0	30.0	41.0
47	35.715952372463335	38.0	35.0	40.0	30.0	41.0
48	35.712104841347475	38.0	35.0	40.0	30.0	41.0
49	35.648417505181456	38.0	35.0	40.0	30.0	41.0
50	35.56786117884509	38.0	35.0	40.0	29.0	41.0
51	35.11934942310286	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	4.0
16	13.0
17	32.0
18	78.0
19	141.0
20	324.0
21	590.0
22	873.0
23	1437.0
24	2733.0
25	4996.0
26	7605.0
27	8826.0
28	8438.0
29	8099.0
30	9182.0
31	10926.0
32	13983.0
33	18669.0
34	35630.0
35	72821.0
36	65041.0
37	48080.0
38	81646.0
39	244840.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.185551141005348	19.277340025949908	24.268589295430555	29.26851953761419
2	33.02086998848221	19.740686943972072	26.788706674582883	20.44973639296283
3	21.424175578873612	21.91821593609564	37.5611737295164	19.096434755514355
4	23.32096811447723	24.66899916135603	34.56251773011166	17.447514994055084
5	19.958021296286248	29.333161780777534	30.746765175037865	19.96205174789835
6	71.6848372860179	1.4999480691811518	22.245147568785082	4.570067076015868
7	71.4893603828309	1.4779356026842807	18.06432910807656	8.968374906408263
8	58.08221811253951	2.7411721483392215	28.241219428636978	10.93539031048429
9	32.82601315477399	18.889331549600133	29.817436043708696	18.46721925191718
10	26.411704431481546	17.181660205025974	44.29419816490437	12.112437198588102
11	23.67533782160291	14.294306677063165	40.40233208130971	21.628023420024213
12	15.842310130850162	21.858999300871663	44.43619407554616	17.86249649273201
13	10.91461798294499	25.660335240563704	42.92167437361356	20.503372402877744
14	8.785144375427265	22.078813931100978	48.75838837741769	20.377653316054065
15	8.594783045440241	18.016738775579803	51.6624837813077	21.72599439767226
16	9.466445715242394	24.493054446750758	40.6190463641451	25.421453473861742
17	12.062831640285294	22.25057317672445	42.90896294929847	22.777632233691786
18	13.981481625016082	29.697142564824386	39.93960523276633	16.381770577393198
19	14.429326806068621	23.6218568290577	36.574023119290516	25.374793245583167
20	11.060024275720094	31.9562106934082	35.033150464509546	21.950614566362162
21	12.586945367228397	24.700777722143766	35.30489591358712	27.40738099704072
22	10.248973397469188	28.462274197823866	31.254292043423465	30.034460361283482
23	9.895068742452592	23.54977375214893	43.77132457691884	22.783832928479637
24	15.590096870354323	21.740566030423707	39.62523000702229	23.04410709219968
25	11.083276881174536	25.561899210806573	39.66258919311909	23.692234714899804
26	14.22795924283316	22.27475588639707	37.96592408179337	25.531360788976404
27	20.9420715591182	22.005800749974032	40.12485098955338	16.927276701354387
28	15.235417128489248	21.67189333564826	35.30319072252046	27.789498813342036
29	16.554459927234845	21.917440849247157	37.11425865268203	24.413840570835962
30	24.690391558374117	22.126094228858342	28.9727463962337	24.210767816533842
31	18.94854818482411	21.799317613538598	37.07674444921554	22.175389752421758
32	19.434372621452233	22.408070824335866	33.06194959145172	25.095606962760176
33	18.632002715904317	24.228129761939826	37.62349071213429	19.516376810021562
34	17.578969723557524	22.750969246104024	36.64455602250232	23.025505007836127
35	17.650122696248115	27.625180401463982	33.25262095617814	21.47207594610976
36	20.643818139822567	29.923467924580947	29.78162703130886	19.651086904287627
37	15.551652562669647	34.57181877229343	33.210921283729846	16.665607381307076
38	17.69321752502368	31.371175140174458	28.975846743627624	21.95976059117424
39	25.358671439134756	25.250779349826146	32.214314613952496	17.176234597086605
40	18.700830428049464	20.193337663485195	38.42694573927009	22.67888616919526
41	21.35426274514059	22.94629113192133	32.209199040752516	23.490247082185558
42	22.336607816905886	16.734745128191612	37.67650665257042	23.25214040233208
43	24.21293805970959	20.798990526888538	35.4723146728591	19.515756740542777
44	15.081639897750543	25.60390891799426	35.51711469270132	23.797336491553878
45	19.15859672076256	23.91918014413515	34.46842218670602	22.453800948396268
46	24.621873880968362	19.75990909781441	33.36981408766852	22.248402933548704
47	18.261511202330222	17.845599599435115	37.793699784060806	26.099189414173857
48	19.940039281401482	15.089390766235356	32.44839084219387	32.5221791101693
49	20.620720551737822	16.252176056327112	39.07057785824902	24.05652553368605
50	19.007144750569303	15.820917733832077	39.08297924782472	26.088958267773904
51	16.06925556008551	14.788192016915493	38.581033004748186	30.561519418250814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	957.0
2	1676.0
3	23088.5
4	44501.0
5	24209.0
6	3917.0
7	3790.0
8	3663.0
9	3877.5
10	4092.0
11	4166.5
12	4241.0
13	4154.5
14	4068.0
15	3736.5
16	3405.0
17	3121.5
18	2838.0
19	2570.0
20	2302.0
21	2202.5
22	2103.0
23	1990.5
24	1878.0
25	1933.5
26	2273.0
27	2557.0
28	2536.0
29	2515.0
30	3214.5
31	3914.0
32	4787.5
33	5661.0
34	6103.5
35	6546.0
36	7508.5
37	8471.0
38	10878.5
39	13286.0
40	18261.5
41	23237.0
42	31928.0
43	40619.0
44	60058.5
45	79498.0
46	100719.0
47	121940.0
48	116831.0
49	111722.0
50	85247.5
51	58773.0
52	44676.5
53	30580.0
54	24039.0
55	17498.0
56	13875.0
57	10252.0
58	9010.5
59	7769.0
60	6617.0
61	5465.0
62	4596.5
63	3728.0
64	3177.0
65	2626.0
66	2246.0
67	1866.0
68	1653.5
69	1441.0
70	1232.5
71	1024.0
72	1080.5
73	1137.0
74	978.5
75	736.0
76	652.0
77	501.5
78	351.0
79	261.5
80	172.0
81	101.5
82	31.0
83	25.0
84	19.0
85	10.0
86	1.0
87	2.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	645089.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.32396363583549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5112548235613	20.53806144811351
2	11.728444465778155	6.643920691072329
3	4.7366618139714864	4.024831109225368
4	2.4447592287529107	2.7698108597428264
5	1.4514449132136482	2.0555336470640886
6	0.9823526121236572	1.669447179801508
7	0.675211520552497	1.3387266588238278
8	0.5094933200582134	1.1544696216023944
9	0.3993451676185331	1.0179934205196564
>10	3.7750124902455573	23.731120754425195
>50	0.5260370230689732	10.10020351803359
>100	0.24507915870159744	12.376239659010457
>500	0.007175741133154881	1.4324112600378192
>1k	0.00607178095882336	2.845279690179903
>5k	0.00110396017433152	1.7540080688020403
>10k+	5.5198008716576E-4	6.547942413545507
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41882	6.492437477619367	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	5745	0.8905747889050969	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	5474	0.8485650817174064	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3813	0.5910812306518946	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	2490	0.3859932505437234	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1809	0.2804264217805605	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	1682	0.2607392158291337	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1563	0.242292148835277	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1533	0.23764162774438874	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	1114	0.17268934984164974	No Hit
CGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	1076	0.16679868979319132	No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1072	0.16617862031440622	No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1042	0.16152809922351802	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1005	0.15579245654475585	No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT	916	0.14199591064178743	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	867	0.13440005952666995	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	825	0.12788932999942643	No Hit
ATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	743	0.11517790568433192	No Hit
GGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	738	0.11440281883585054	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	728	0.1128526451388878	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	703	0.10897721089648095	No Hit
ATAGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	657	0.10184641189045233	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8722827392809365	0.0
2	0.0	0.0	0.0	2.8512344808235763	0.0
3	0.0	0.0	0.0	4.771589656621025	0.0
4	0.0	0.0	0.0	6.051878112942555	0.0
5	0.0	0.0	0.0	12.0501202159702	0.0
6	0.0	0.0	0.0	13.705550706956714	0.0
7	0.0	0.0	0.0	19.329580879537552	0.0
8	0.0	0.0	0.0	25.984011508489527	0.0
9	0.0	0.0	0.0	31.323894842417094	0.0
10	0.0	0.0	0.0	34.04723999324124	0.0
11	0.0	0.0	0.0	36.499149729727215	0.0
12	0.0	0.0	0.0	38.2809193770162	0.0
13	0.0	0.0	0.0	38.95679510889195	0.0
14	0.0	0.0	0.0	39.44292958025947	0.0
15	0.0	0.0	0.0	40.95961952536782	0.0
16	0.0	0.0	0.0	42.71426113295995	0.0
17	0.0	0.0	0.0	45.022469767737476	0.0
18	0.0	0.0	0.0	45.97985704298166	0.0
19	0.0	0.0	0.0	47.130085926128025	0.0
20	0.0	0.0	0.0	48.17226770259607	0.0
21	3.100347393925489E-4	0.0	0.0	49.18747645673698	0.0
22	4.650521090888234E-4	0.0	0.0	50.20377033246575	0.0
23	4.650521090888234E-4	0.0	0.0	50.92723639683827	0.0
24	0.0038754342424068616	0.0	0.0	51.55071625775668	0.0
25	0.0038754342424068616	0.0	0.0	52.08103067948764	0.0
26	0.0038754342424068616	0.0	0.0	52.67242194487893	0.0
27	0.0038754342424068616	0.0	0.0	53.191265081252354	0.0
28	0.00449550372119196	0.0	0.0	53.69135111589254	0.0
29	0.00449550372119196	0.0	0.0	54.344904346532026	0.0
30	0.00449550372119196	0.0	0.0	54.8952160089538	0.0
31	0.00449550372119196	0.0	0.0	55.365383691242606	0.0
32	0.00449550372119196	0.0	0.0	55.83694652985867	0.0
33	0.00449550372119196	0.0	0.0	56.28432665880212	0.0
34	0.00449550372119196	0.0	0.0	56.83169299119967	0.0
35	0.00449550372119196	0.0	0.0	57.27767796381585	0.0
36	0.00449550372119196	0.0	0.0	57.69777503569275	0.0
37	0.00449550372119196	0.0	0.0	58.10407556166668	0.0
38	0.00449550372119196	0.0	0.0	58.52339754669511	0.0
39	0.00449550372119196	0.0	0.0	58.924272464729675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTTA	20	7.0309814E-4	45.000004	32
CTTCGCT	20	7.0309814E-4	45.000004	2
TAGAGTA	30	2.1640153E-6	45.000004	33
AACCGTA	25	3.888895E-5	45.000004	37
TTGGCTA	20	7.0309814E-4	45.000004	19
CGGAACA	30	2.1640153E-6	45.000004	27
AACCGCG	25	3.888895E-5	45.000004	19
CTGTCGG	25	3.888895E-5	45.000004	1
TCGTTGA	20	7.0309814E-4	45.000004	23
CTCACGG	50	2.1827873E-11	45.000004	1
GGTACGA	20	7.0309814E-4	45.000004	7
GTACCGG	25	3.888895E-5	45.000004	1
TCGCAGG	65	0.0	45.000004	1
ACACGAC	30	2.1640153E-6	45.000004	25
ACTGCAC	20	7.0309814E-4	45.000004	21
GGTAATC	25	3.888895E-5	45.000004	7
TTAATCG	20	7.0309814E-4	45.000004	19
CGCGGGT	30	2.1640153E-6	45.000004	3
GACACGA	30	2.1640153E-6	45.000004	24
TAGACAG	25	3.888895E-5	45.000004	42
>>END_MODULE