FastQCFastQC Report
Thu 26 May 2016
SRR1043226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043226_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1417012
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT734045.180196074556885No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT85420.60281776018834TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT81570.5756479126499988TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT50720.3579362771804332TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC47440.3347889784984178TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG30450.21488879416688075No Hit
TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT29900.2110073873756891TruSeq Adapter, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT27530.19428205265728166No Hit
ACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC21930.1547622744196944TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC20740.1463643215442071No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT18910.13344982258442412No Hit
CGTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG17640.12448730144839987No Hit
GCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC17590.12413444628556426TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC17430.12300530976449035TruSeq Adapter, Index 15 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC16640.11743019819168785TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT16330.11524249618210713No Hit
ACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT15220.10740911156715681TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAA253.4175617E-546.031
CACGGCA206.311955E-446.03
TAACGGT301.861712E-646.019
CTAACGG650.046.01
TACGCCC206.311955E-446.037
CCGACTC206.311955E-446.034
ACGTCCC206.311955E-446.014
TCATCGG551.8189894E-1246.01
TATAGCG206.311955E-446.01
CGTCCCA206.311955E-446.015
ACGGTTT206.311955E-446.04
CCTACGG351.0197982E-745.9999961
GTCACGG950.045.9999961
TGTCGAT351.0197982E-745.99999646
TATCCGG700.045.9999961
CGTTTTT177050.045.662241
AATGCGG2300.045.01
TTACCGG900.043.4444471
TACGCGG1650.043.212121
ACGATGG750.042.9333341
Produced by FastQC (version 0.11.5)