SRR1043225_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043225_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1385358
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69234	4.99755297908555	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	8128	0.5867075514054851	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	7890	0.5695278765488776	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	5085	0.36705313716743254	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	4706	0.3396955877109022	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCC	2953	0.2131578985359741	No Hit
TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2937	0.21200296241116018	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	2547	0.1838513943688202	No Hit
ACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	2105	0.1519462839208349	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT	1979	0.14285116193792508	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT	1826	0.13180708524439172	No Hit
GCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	1795	0.12956939650256466	No Hit
AGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	1670	0.12054645802745571	No Hit
CGTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT	1668	0.12040209101185397	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC	1618	0.11679291562181038	No Hit
TCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	1583	0.11426649284877989	No Hit
TGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT	1515	0.1093580143183206	TruSeq Adapter, Index 14 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	1488	0.10740905960769707	TruSeq Adapter, Index 15 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGC	20	7.033788E-4	45.000004	9
AGCGTAG	20	7.033788E-4	45.000004	35
CCGCTTA	20	7.033788E-4	45.000004	34
GCCGATT	30	2.1658252E-6	45.000004	8
CCCGTAG	20	7.033788E-4	45.000004	23
ACGTGTC	20	7.033788E-4	45.000004	9
TCGTCGT	20	7.033788E-4	45.000004	44
ACGTCCG	20	7.033788E-4	45.000004	39
ATAACGG	115	0.0	45.000004	1
CCACGTC	20	7.033788E-4	45.000004	37
GCGTCAT	40	6.8175723E-9	45.000004	36
GCCCGTA	20	7.033788E-4	45.000004	22
GTCGGCG	20	7.033788E-4	45.000004	3
ACGTTGG	70	0.0	45.0	1
GTCCTCG	25	3.891223E-5	45.0	22
GGCGTAG	25	3.891223E-5	45.0	7
CCTACGG	35	1.2121745E-7	45.0	1
GTGATAC	35	1.2121745E-7	45.0	8
ACCACGG	25	3.891223E-5	45.0	1
CATCCGG	45	3.8562575E-10	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content